重金属对浒苔吸收硝酸盐的动力学特性影响
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摘要
为探讨大型海藻对重金属胁迫的响应,选取浒苔(Ulva prolifera)为实验材料,通过实验室一次性培养实验,从大型藻营养盐吸收动力学角度,研究了Cu(II)、Zn(II)和Pb(II)对浒苔吸收硝酸盐动力学参数的影响.结果表明:较低浓度的Cu(II)(<0.04mg/L)和Zn(II)(<0.12mg/L)对浒苔吸收硝酸盐具有促进作用;当达到一定浓度(Cu(II)>0.10mg/L和Zn(II)>0.20mg/L)时则对浒苔吸收硝酸盐表现为明显的抑制作用,Pb(II)对浒苔吸收硝酸盐均是抑制作用.Cu(II)、Zn(II)和Pb(II)对浒苔吸收硝酸盐的最大吸收速率(Vmax)抑制率IPC50分别为0.23,0.66,0.63mg/L.当添加重金属浓度大于0.50mg/L时,重金属对浒苔吸收硝酸盐的抑制顺序为:Cu(II)最强,Pb(II)次之,Zn(II)最小.
In order to investigate the response of the macroalgae to heavy metal contamination, the influence of three heavy metal ions(Cu(II), Zn(II) and Pb(II)) on the uptake of nitrate by Ulva prolifera was investigated. Kinetic parameters related to nitrate uptake in different trials were determined by the batch culture experiment in the laboratory. The results showed that the uptake of nitrate by Ulva prolifera was promoted at low concentrations of Cu(II)(<0.04mg/L) and Zn(II)(<0.12mg/L) and inhibited at high concentrations(Cu(II)>0.10mg/L and Zn(II)>0.20mg/L). Pb(II) played a negative role in the uptake of nitrate by Ulva prolifera at all tested concentrations. The inhibition rate of the maximum absorption rate of nitrate(Vmax) were calculated to be 0.23mg/L, 0.66mg/L, and 0.63mg/L for Cu(II), Zn(II), and Pb(II), respectively. The inhibition effects of the three heavy metals at concentrations >0.5mg/L on Ulva prolifera nitrate uptake were observed to be in the order of Cu(II)>Pb(II)>Zn(II).
In order to investigate the response of the macroalgae to heavy metal contamination, the influence of three heavy metal ions(Cu(II), Zn(II) and Pb(II)) on the uptake of nitrate by Ulva prolifera was investigated. Kinetic parameters related to nitrate uptake in different trials were determined by the batch culture experiment in the laboratory. The results showed that the uptake of nitrate by Ulva prolifera was promoted at low concentrations of Cu(II)(<0.04mg/L) and Zn(II)(<0.12mg/L) and inhibited at high concentrations(Cu(II)>0.10mg/L and Zn(II)>0.20mg/L). Pb(II) played a negative role in the uptake of nitrate by Ulva prolifera at all tested concentrations. The inhibition rate of the maximum absorption rate of nitrate(Vmax) were calculated to be 0.23mg/L, 0.66mg/L, and 0.63mg/L for Cu(II), Zn(II), and Pb(II), respectively. The inhibition effects of the three heavy metals at concentrations >0.5mg/L on Ulva prolifera nitrate uptake were observed to be in the order of Cu(II)>Pb(II)>Zn(II).
引文
[1]Liu D,Keesing J K,He P,et al.The world's largest macroalgal bloom in the Yellow Sea,China:Formation and implications[J].Estuarine Coastal and Shelf Science,2013,129:2-10.
[2]Li H M,Zhang C S,Han X R,et al.Changes in concentrations of oxygen,dissolved nitrogen,phosphate,and silicate in the southern Yellow Sea,1980-2012:Sources and seaward gradients1[J].Estuarine Coastal and Shelf Science,2015,163:44-55.
[3]Wang Wenxiong,Robert C H Dei,Yan Xu.Response of Zn assimilation by coastal plankton to macronutrients[J].Limnology and Oceanography,2001,46(6):1524-1534.
[4]Luoma S N,Alexer V G,Byeong Gweon L,et al.Metal uptake by phytoplankton during a bloom in South San Francisco Bay:Implications for metal cycling in estuaries[J].Limnology and Oceanography,1998,43(5):1007-1016.
[5]Fowler S W.Critical review of selected heavy metal and chlorinated hydrocarbon concentrations in the marine environment[J].Marine Environmental Research,1990,29(1):1-64.
[6]朱喜锋.重金属汞、铜和镉对三种大型经济海藻毒性效应的研究[D].汕头:汕头大学,2010.
[7]颜海波.江蓠属海藻在N营养盐和重金属Pb、Cd胁迫下的生理生化响应及蛋白质组学研究[D].汕头:汕头大学,2010.
[8]吴婷,赵乐毅,刘浩涤,等.浒苔对营养盐影响的初步研究[J].海洋环境科学,2013,32(3):347-352.
[9]高素兰.营养盐和微量元素与黄骅赤潮的相关性[J].海洋科学进展,1997,15(2):59-63.
[10]华泽爱.赤潮灾害[M].北京:海洋出版社,1994,50-58.
[11]王增焕,林钦,李刘冬,等.大型海藻对重金属镉、铜的富集动力学研究[J].中国环境科学,2013,33(1):154-160.
[12]彭克俭,刘益贵,沈振国,等.镉、铅在沉水植物龙须眼子菜叶片中的分布[J].中国环境科学,2010,30(Suppl.):69-74.
[13]曾阿妍,颜昌宙,金相灿,等.金鱼藻对Cu2+的生物吸附特征[J].中国环境科学,2006,26(6):691-694.
[14]李国新,张丹丹,颜昌宙,等.轮叶黑藻对铅的吸附特征及生物吸附机理研究[J].中国环境科学,2011,31(8):1327-1333.
[15]魏海峰,朱学惠,刘长发,等.Pb(II)、Zn(II)对孔石莼吸收总氨氮的影响[J].大连海洋大学学报,2008,23(4):283-287.
[16]张寒野,吴望星,宋丽珍,等.条浒苔海区试栽培及外界因子对藻体生长的影响[J].中国水产科学,2006,13(5):781-786.
[17]吴婷.营养盐对浒苔生长的影响及浒苔对不同氮源吸收特性的初步研究[D].青岛:中国海洋大学,2013.
[18]Dugdale R C,Goering J J.Uptake of new and regenerated forms of nitrogen in primary productivity[J].Limnology and Oceanography,1967,12(2):196-206.
[19]Ewijk P H V,Hoekstra J A.Calculation of the EC50 and its confidence interval when subtoxic stimulus is present[J].Ecotoxicology and Environmental Safety,1993,25(1):25-32.
[20]Linder M C.Biochemistry of copper[J].Unknown,1991,10(3):687-703.
[21]Halliwell B,Gutteridge J M C.Free radicals in biology and medicine[J].Journal of Free radicals in biology and medicine,1999,1(4):331-332.
[22]Webster E A,Gadd G M.Perturbation of monovalent cation composition in Ulva lactuca by cadmium,copper and zinc[J].Biometals,1996,9(1):51-56.
[23]Lidon F C,Henriques F S.Role of rice shoot vacuoles in copper toxicity regulation[J].Environmental and Experimental Botany,1998,39(3):197-202.
[24]Collén J,Pinto E,Pedersén M,et al.Induction of oxidative stress in the red macroalga Gracilaria tenuistipitata by pollutant metals[J].Archives of Environmental Contam ination and Toxicology,2003,45(3):337-42.
[25]Han T,Kang S H,Park J S,et al.Physiological responses of Ulva pertusa and U.armoricana to copper exposure[J].Aquatic Toxicology,2008,86(2):176-184.
[26]姜彬慧,林碧琴.重金属对藻类的毒性作用研究进展[J].辽宁大学学报(自然科学版),2000,27(3):281-287.
[27]Barbara Pawlik-Skowrońska.Phytochelatin production in freshwater algae Stigeoclonium in response to heavy metals contained in mining water:Effects of some environmental factors[J].Aquatic Toxicology,2001,52(3/4):241-249.
[28]王东军,陈立军.重金属对藻类的毒害作用[J].有色矿冶,2005,21(3):40-41.
[29]邓鹜远,罗言云,吴传芳,等.Ag+、Pb2+对轮藻光合色素及GAP脱氢酶的影响[J].四川大学学报(自然科学版),2005,(4):822-826.
[30]朱明,郭赣林,刘兆普,等.浒苔对重金属pb2+的生物吸附及其生理反应[J].水产科学,2011,30(11):681-684.
[31]王修林,张莹莹,杨茹君,等.不同浓度的营养盐(NO3^-N,PO4^-P)条件下Pb(Ⅱ)对2种海洋赤潮藻生长影响的研究[J].中国海洋大学学报(自然科学版),2005,35(1):133-136.
[32]吴瑜端,郑志宏.海洋重金属生物地球化学与海洋环境保护[J].海洋环境科学,1987,6(3):38-48.
[33]战玉杰,杨茹君,王修林,等.Hg(Ⅱ)和Pb(Ⅱ)对海洋单细胞藻的急性毒性效应[J].生态毒理学报,2011,6(5):523-531.
[34]Liu D,Keesing J K,Dong Z,et al.Recurrence of the world’s largest green-tide in 2009in Yellow Sea,China:Porphyra yezoensis aquaculture rafts confirmed as nursery for macroalgal blooms[J].Marine Pollution Bulletin,2010,60(9):1423-1432.
[35]Liu D,Keesing J K,Xing Q,et al.World's largest macroalgal bloom caused by expansion of seaweed aquaculture in China.[J].,Marine Pollution Bulletin,2009,58(6):888-895.
[36]Keesing J K,Liu D,Fearns P,et al.Inter-and intra-annual patterns of Ulva prolifera green tides in the Yellow Sea during 2007-2009,their origin and relationship to the expansion of coastal seaweed aquaculture in China[J].Marine Pollution Bulletin,2011,62(6):1169-1182.
[37]田琳,陈洪涛,杜俊涛,等.北黄海表层海水溶解态重金属的分布)特征及其影响因素[J].中国海洋大学学报(自然科学版),2009,(4):617-621.
[38]史华明,王丽莎,石晓勇.北黄海溶解无机氮组成、分布及季节变.化特征[J].中国海洋大学学报(自然科学版),2009,(S1):207-211.
[39]Shi X,Qi M,Tang H,et al.Spatial and temporal nutrient variations in the Yellow Sea and their effects on Ulva prolifera blooms[J].s Estuarine Coastal and Shelf Science,2015,163:36-43.
[2]Li H M,Zhang C S,Han X R,et al.Changes in concentrations of oxygen,dissolved nitrogen,phosphate,and silicate in the southern Yellow Sea,1980-2012:Sources and seaward gradients1[J].Estuarine Coastal and Shelf Science,2015,163:44-55.
[3]Wang Wenxiong,Robert C H Dei,Yan Xu.Response of Zn assimilation by coastal plankton to macronutrients[J].Limnology and Oceanography,2001,46(6):1524-1534.
[4]Luoma S N,Alexer V G,Byeong Gweon L,et al.Metal uptake by phytoplankton during a bloom in South San Francisco Bay:Implications for metal cycling in estuaries[J].Limnology and Oceanography,1998,43(5):1007-1016.
[5]Fowler S W.Critical review of selected heavy metal and chlorinated hydrocarbon concentrations in the marine environment[J].Marine Environmental Research,1990,29(1):1-64.
[6]朱喜锋.重金属汞、铜和镉对三种大型经济海藻毒性效应的研究[D].汕头:汕头大学,2010.
[7]颜海波.江蓠属海藻在N营养盐和重金属Pb、Cd胁迫下的生理生化响应及蛋白质组学研究[D].汕头:汕头大学,2010.
[8]吴婷,赵乐毅,刘浩涤,等.浒苔对营养盐影响的初步研究[J].海洋环境科学,2013,32(3):347-352.
[9]高素兰.营养盐和微量元素与黄骅赤潮的相关性[J].海洋科学进展,1997,15(2):59-63.
[10]华泽爱.赤潮灾害[M].北京:海洋出版社,1994,50-58.
[11]王增焕,林钦,李刘冬,等.大型海藻对重金属镉、铜的富集动力学研究[J].中国环境科学,2013,33(1):154-160.
[12]彭克俭,刘益贵,沈振国,等.镉、铅在沉水植物龙须眼子菜叶片中的分布[J].中国环境科学,2010,30(Suppl.):69-74.
[13]曾阿妍,颜昌宙,金相灿,等.金鱼藻对Cu2+的生物吸附特征[J].中国环境科学,2006,26(6):691-694.
[14]李国新,张丹丹,颜昌宙,等.轮叶黑藻对铅的吸附特征及生物吸附机理研究[J].中国环境科学,2011,31(8):1327-1333.
[15]魏海峰,朱学惠,刘长发,等.Pb(II)、Zn(II)对孔石莼吸收总氨氮的影响[J].大连海洋大学学报,2008,23(4):283-287.
[16]张寒野,吴望星,宋丽珍,等.条浒苔海区试栽培及外界因子对藻体生长的影响[J].中国水产科学,2006,13(5):781-786.
[17]吴婷.营养盐对浒苔生长的影响及浒苔对不同氮源吸收特性的初步研究[D].青岛:中国海洋大学,2013.
[18]Dugdale R C,Goering J J.Uptake of new and regenerated forms of nitrogen in primary productivity[J].Limnology and Oceanography,1967,12(2):196-206.
[19]Ewijk P H V,Hoekstra J A.Calculation of the EC50 and its confidence interval when subtoxic stimulus is present[J].Ecotoxicology and Environmental Safety,1993,25(1):25-32.
[20]Linder M C.Biochemistry of copper[J].Unknown,1991,10(3):687-703.
[21]Halliwell B,Gutteridge J M C.Free radicals in biology and medicine[J].Journal of Free radicals in biology and medicine,1999,1(4):331-332.
[22]Webster E A,Gadd G M.Perturbation of monovalent cation composition in Ulva lactuca by cadmium,copper and zinc[J].Biometals,1996,9(1):51-56.
[23]Lidon F C,Henriques F S.Role of rice shoot vacuoles in copper toxicity regulation[J].Environmental and Experimental Botany,1998,39(3):197-202.
[24]Collén J,Pinto E,Pedersén M,et al.Induction of oxidative stress in the red macroalga Gracilaria tenuistipitata by pollutant metals[J].Archives of Environmental Contam ination and Toxicology,2003,45(3):337-42.
[25]Han T,Kang S H,Park J S,et al.Physiological responses of Ulva pertusa and U.armoricana to copper exposure[J].Aquatic Toxicology,2008,86(2):176-184.
[26]姜彬慧,林碧琴.重金属对藻类的毒性作用研究进展[J].辽宁大学学报(自然科学版),2000,27(3):281-287.
[27]Barbara Pawlik-Skowrońska.Phytochelatin production in freshwater algae Stigeoclonium in response to heavy metals contained in mining water:Effects of some environmental factors[J].Aquatic Toxicology,2001,52(3/4):241-249.
[28]王东军,陈立军.重金属对藻类的毒害作用[J].有色矿冶,2005,21(3):40-41.
[29]邓鹜远,罗言云,吴传芳,等.Ag+、Pb2+对轮藻光合色素及GAP脱氢酶的影响[J].四川大学学报(自然科学版),2005,(4):822-826.
[30]朱明,郭赣林,刘兆普,等.浒苔对重金属pb2+的生物吸附及其生理反应[J].水产科学,2011,30(11):681-684.
[31]王修林,张莹莹,杨茹君,等.不同浓度的营养盐(NO3^-N,PO4^-P)条件下Pb(Ⅱ)对2种海洋赤潮藻生长影响的研究[J].中国海洋大学学报(自然科学版),2005,35(1):133-136.
[32]吴瑜端,郑志宏.海洋重金属生物地球化学与海洋环境保护[J].海洋环境科学,1987,6(3):38-48.
[33]战玉杰,杨茹君,王修林,等.Hg(Ⅱ)和Pb(Ⅱ)对海洋单细胞藻的急性毒性效应[J].生态毒理学报,2011,6(5):523-531.
[34]Liu D,Keesing J K,Dong Z,et al.Recurrence of the world’s largest green-tide in 2009in Yellow Sea,China:Porphyra yezoensis aquaculture rafts confirmed as nursery for macroalgal blooms[J].Marine Pollution Bulletin,2010,60(9):1423-1432.
[35]Liu D,Keesing J K,Xing Q,et al.World's largest macroalgal bloom caused by expansion of seaweed aquaculture in China.[J].,Marine Pollution Bulletin,2009,58(6):888-895.
[36]Keesing J K,Liu D,Fearns P,et al.Inter-and intra-annual patterns of Ulva prolifera green tides in the Yellow Sea during 2007-2009,their origin and relationship to the expansion of coastal seaweed aquaculture in China[J].Marine Pollution Bulletin,2011,62(6):1169-1182.
[37]田琳,陈洪涛,杜俊涛,等.北黄海表层海水溶解态重金属的分布)特征及其影响因素[J].中国海洋大学学报(自然科学版),2009,(4):617-621.
[38]史华明,王丽莎,石晓勇.北黄海溶解无机氮组成、分布及季节变.化特征[J].中国海洋大学学报(自然科学版),2009,(S1):207-211.
[39]Shi X,Qi M,Tang H,et al.Spatial and temporal nutrient variations in the Yellow Sea and their effects on Ulva prolifera blooms[J].s Estuarine Coastal and Shelf Science,2015,163:36-43.