特克赛尔羊JIVET技术研究
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摘要
探讨特克赛尔羊JIVET(juvenile in vitro embryo technology)技术,为优种绵羊JIVET技术体系完善提供技术支撑。试验对4~8周龄特克赛尔羊超数排卵,采用活体采卵的方法及对卵母细胞的体外成熟—受精—受精卵培养—受精卵移植的方法进行特克赛尔羊JIVET技术研究。结果显示:4只4~8周龄特克赛尔羊获得可用卵母细胞296枚,平均74枚/只;获得2~4细胞期受精卵205枚,卵裂率为69.26%,平均获2~4细胞期受精卵51.25枚/只;将66枚2~4细胞期受精卵移植给11只受体羊,6只羊受孕,生产11只羔羊,受胎率为54.55%。研究表明,本试验方法可作为特克赛尔羊JIVET技术研究的方法。
The present study was to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on raspberry sheep. The methods of superovulation,ovum pick-up and oocytes-in-vitro-maturation-fertilization-fertilized-embryo culture-transplantation were used on 4-8 week old Texel sheep. The results were that 296 pieces of available oocytes were obtained at an average of 74 pieces per sheep; 205 fertilized eggs were obtained at the 2 ~ 4 cell stage at an average of 51. 25 pieces per sheep and a cleavage rate of 69. 26%; 66 of the fertilized eggs were transplanted to 11 receptor sheep. Six sheep were conceived at a conception rate of 54. 55%,and 11 lambs were produced. These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.
The present study was to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on raspberry sheep. The methods of superovulation,ovum pick-up and oocytes-in-vitro-maturation-fertilization-fertilized-embryo culture-transplantation were used on 4-8 week old Texel sheep. The results were that 296 pieces of available oocytes were obtained at an average of 74 pieces per sheep; 205 fertilized eggs were obtained at the 2 ~ 4 cell stage at an average of 51. 25 pieces per sheep and a cleavage rate of 69. 26%; 66 of the fertilized eggs were transplanted to 11 receptor sheep. Six sheep were conceived at a conception rate of 54. 55%,and 11 lambs were produced. These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep.
引文
[1]Kelly J M,Kleemann D O,Walker S K.Enhanced efficiency in the production of offspring from 4-to 8-week-old lambs[J].Theriogenology,2005,63(7):1876-1890.
[2]Rangel-santos R,Mcdonald M F,Wickham G A.Evaluation of the feasibility of a juvenile MOET scheme in sheep[J].Proceedings of The New Zealand Society of Animal Production,1991,51(1):139-142.
[3]Kelly J M,Kleemann D O,Walker S K.The effect of nutrition during pregnancy on the in vitro production of embryos from resulting lambs[J].Theriogenol,2005,63(7):2020-2031.
[4]Bai J H,Hou J,Guan H,et al.Effect of 2-mercaptoethanol and cysteine supplementation during in vitro maturation on the developmental competence of oocytes from hormone-stimulated lambs[J].Theriogenol,2008,70(5):758-764.
[5]Gou K M,Guan H,Bai J H,et al.Field evaluation of juvenile in vitro embryo transfer(JIVET)in sheep[J].Anim Reprod Sci,2009,112(3/4):316-324.
[6]白佳桦.性成熟前绵羊、牛JIVET技术研究[D].北京:中国农业大学,2008.
[7]汪立芹,何宗霖,林嘉鹏,等.萨福克羔羊卵泡诱导发育效果的研究[J].中国农业大学学报,2015,20(4):141-146.
[8]郭洪,万鹏程,石文艳,等.不同品种绵羊超数排卵及胚胎体外生产技术研究[J].畜牧与兽医,2012,44(10):20-24.
[9]刘宗正,李和刚,程明,等.利用JIVET技术快速繁育萨福克羊的研究[J].畜牧与饲料科学,2016,37(3):1-4.
[10]马世科,乔海生,马利青,等.幼畜超数排卵及体外胚胎生产技术在青海高原地区的应用研究[J].青海畜牧兽医杂志,2015,45(2):13-14.
[11]陈晓勇,田树军,桑润滋,等.诱导幼羔卵泡发育及体外胚胎生产[J].农业生物技术学报,2008,16(3):456-460.
[12]张兴会,张世伟,宋先忱,等.幼龄母羊超数排卵及卵母细胞成熟效果分析[J].中国畜牧兽医,2011,38(11):136-139.
[13]吴海风,李发弟,常卫华,等.羔羊超数排卵及体外受精技术研究[J].甘肃农业大学学报,2011,46(6):1-5.
[14]李运生,曹鸿国,刘亚,等.羔羊超数排卵与胚胎体外生产技术研究进展[J].动物医学进展,2011,32(11):99-104.
[15]郭宏,万鹏程,代蓉,等.羔羊卵母细胞体外胚胎生产影响因素分析[J].湖北农业科学,2013,52(19):4726-4729.
[16]闵江涛,杜卫华,赵学明,等.绵羊卵母细胞采集、体外成熟和胚胎体外培养对体外受精技术效率的影响[J].中国畜牧兽医,2014,41(5):202-207.
[17]毛杨毅,罗惠娣,贺东昌等.不同季节绒山羊同期发情效果研究[J].家畜生态学报,2012,33(5):42-45.
[18]于勇,钟旭,王芬露,等.不同月龄绵羔羊卵泡发育的比较研究[J].中国畜牧杂志,2012,48(9):17-21.
[19]吴伟伟,徐新明,张廷虎,等.影响新疆细毛羊羔羊超排及体外受精效果的研究[J].中国畜牧兽医,2011,38(4):144-147.
[20]郭洪,万鹏程,石文艳,等.EGF和β-巯基乙醇对羔羊卵母细胞体外受精和体外发育的影响[J].安徽农业科学,2012,40(11):6537-6540.
[2]Rangel-santos R,Mcdonald M F,Wickham G A.Evaluation of the feasibility of a juvenile MOET scheme in sheep[J].Proceedings of The New Zealand Society of Animal Production,1991,51(1):139-142.
[3]Kelly J M,Kleemann D O,Walker S K.The effect of nutrition during pregnancy on the in vitro production of embryos from resulting lambs[J].Theriogenol,2005,63(7):2020-2031.
[4]Bai J H,Hou J,Guan H,et al.Effect of 2-mercaptoethanol and cysteine supplementation during in vitro maturation on the developmental competence of oocytes from hormone-stimulated lambs[J].Theriogenol,2008,70(5):758-764.
[5]Gou K M,Guan H,Bai J H,et al.Field evaluation of juvenile in vitro embryo transfer(JIVET)in sheep[J].Anim Reprod Sci,2009,112(3/4):316-324.
[6]白佳桦.性成熟前绵羊、牛JIVET技术研究[D].北京:中国农业大学,2008.
[7]汪立芹,何宗霖,林嘉鹏,等.萨福克羔羊卵泡诱导发育效果的研究[J].中国农业大学学报,2015,20(4):141-146.
[8]郭洪,万鹏程,石文艳,等.不同品种绵羊超数排卵及胚胎体外生产技术研究[J].畜牧与兽医,2012,44(10):20-24.
[9]刘宗正,李和刚,程明,等.利用JIVET技术快速繁育萨福克羊的研究[J].畜牧与饲料科学,2016,37(3):1-4.
[10]马世科,乔海生,马利青,等.幼畜超数排卵及体外胚胎生产技术在青海高原地区的应用研究[J].青海畜牧兽医杂志,2015,45(2):13-14.
[11]陈晓勇,田树军,桑润滋,等.诱导幼羔卵泡发育及体外胚胎生产[J].农业生物技术学报,2008,16(3):456-460.
[12]张兴会,张世伟,宋先忱,等.幼龄母羊超数排卵及卵母细胞成熟效果分析[J].中国畜牧兽医,2011,38(11):136-139.
[13]吴海风,李发弟,常卫华,等.羔羊超数排卵及体外受精技术研究[J].甘肃农业大学学报,2011,46(6):1-5.
[14]李运生,曹鸿国,刘亚,等.羔羊超数排卵与胚胎体外生产技术研究进展[J].动物医学进展,2011,32(11):99-104.
[15]郭宏,万鹏程,代蓉,等.羔羊卵母细胞体外胚胎生产影响因素分析[J].湖北农业科学,2013,52(19):4726-4729.
[16]闵江涛,杜卫华,赵学明,等.绵羊卵母细胞采集、体外成熟和胚胎体外培养对体外受精技术效率的影响[J].中国畜牧兽医,2014,41(5):202-207.
[17]毛杨毅,罗惠娣,贺东昌等.不同季节绒山羊同期发情效果研究[J].家畜生态学报,2012,33(5):42-45.
[18]于勇,钟旭,王芬露,等.不同月龄绵羔羊卵泡发育的比较研究[J].中国畜牧杂志,2012,48(9):17-21.
[19]吴伟伟,徐新明,张廷虎,等.影响新疆细毛羊羔羊超排及体外受精效果的研究[J].中国畜牧兽医,2011,38(4):144-147.
[20]郭洪,万鹏程,石文艳,等.EGF和β-巯基乙醇对羔羊卵母细胞体外受精和体外发育的影响[J].安徽农业科学,2012,40(11):6537-6540.