大白菜对根肿病抗性的鉴定方法
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摘要
以采自阿城区阿什河办事处南城村的大白菜活体病样为接种病原,以大白菜感病品种"东农909"为试材,采用L9(3)4正交设计,分析了病菌活化基质、病菌活化时间、移植基质和移植后调查时间等4个因素对根肿病发病的影响,以期建立大白菜抗根肿病鉴定技术体系。结果表明:最佳病菌活化基质为全草炭,最佳病菌活化时间为10d,移植后34d调查发病最重。该试验为大白菜抗根肿病育种提供了一套相对可靠的鉴定方法,可为十字花科蔬菜抗病鉴定提供参考。
In order to establish a set of methods for identification of Chinese cabbage resistance to clubroot,the pathogen of clubroot was obtained from Chinese cabbage field in Nancheng village of Ashi River Subdistrict Office,Acheng district,and a Chinese cabbage variety ‘Dongnong 909'was used as experimental material.The effect of four factors on the incidence of clubrot was analysed in L9(3)4 orthogonal test,which included activate matrix of the pathogen,the pathogen activation time,transplant matrix of seedling and investigation time after seedling transplanted.The results showed that the best substrate of pathogen activation was turfy soil,the incidence was the most severe in 10 days after pathogen activation,the best investigation time was 34 days after post-transplant.The research would provide a set of relatively effective methods for identification of Chinese cabbage resistance to clubroot,and provide some suggestions for identification of other brassicaeous vegetable resistance to clubroot.
In order to establish a set of methods for identification of Chinese cabbage resistance to clubroot,the pathogen of clubroot was obtained from Chinese cabbage field in Nancheng village of Ashi River Subdistrict Office,Acheng district,and a Chinese cabbage variety ‘Dongnong 909'was used as experimental material.The effect of four factors on the incidence of clubrot was analysed in L9(3)4 orthogonal test,which included activate matrix of the pathogen,the pathogen activation time,transplant matrix of seedling and investigation time after seedling transplanted.The results showed that the best substrate of pathogen activation was turfy soil,the incidence was the most severe in 10 days after pathogen activation,the best investigation time was 34 days after post-transplant.The research would provide a set of relatively effective methods for identification of Chinese cabbage resistance to clubroot,and provide some suggestions for identification of other brassicaeous vegetable resistance to clubroot.
引文
[1] SONG T,CHU M,LAHLALI R,et al.Clubroot in the crops:The interaction between Plasmodioporo brassicae and Brassica oleracea[J].Frontiers in Plant Science,2016(7):1013.
[2] DONALD C,PORTER I.Integrated control of clubroot[J].J Plant Growth Regul,2009(28):289-303.
[3]孙良菲,任佐华,彭沙莎,等.白菜根肿病菌拮抗微生物的筛选及防治效果评价[J].湖南农业科学,2013(1):84-87.
[4] ARIE T,KOBAYASHI Y,OKADA K,et al.Control of soilborne club-root disease of cruciferous plants by epoxydon from Phoma glomerata[J].Plant Pathology,1998,47:743-748.
[5] CHEAH L H,VEERAKONE S,KENT G.Biological control of clubroot on cauliflower with Trichodermaand Streptomyces spp.[J].New Zealand Plant Protection,2000,53:18-21.
[6]胡靖锋,吴丽艳,林良斌,等.用菌土接种法鉴定云南省主要十字花科作物对根肿病的抗性[J].中国蔬菜,2010(14):71-74.
[7]黄蓉,黄瑞荣,华菊玲,等.小白菜根肿病的接种方法及条件研究[J].中国农学通报,2012,28(31):252-255.
[8]李晓鸥,吴飞燕,钟惠宏,等.大白菜根肿病苗期人工接种抗病性鉴定技术初报[J].中国蔬菜,1990(6):9-10.
[9]李茜,沈向群,耿新翠,等.芸薹根肿菌(Plasmodiophora brassicae)单孢分离接种及生理小种的鉴定[J].植物保护,2012,38(3):95-101.
[10]司军,李成琼,肖崇刚,等.甘蓝根肿病接种方法的研究[J].西南农业大学学报(自然科学版),2003(3):216-219.
[11]王树源,李晏,张毅科.混凝土正交试验结果极差分析与方差分析方法对比[J].建材发展导向,2016,44(12):44-48.
[12]李妍,谢学文,向文胜,等.白菜根肿病的接种方法[J].植物保护学报,2011,38(1):95-96.
[13]唐文华,李晖,孙俊.北京白菜根肿病发生的生态条件及其防治研究[J].北京农业科学,1990(6):6-9.
[14]张淑霞,杨晓云,司朝光,等.大白菜根肿病人工接种鉴定方法比较[J].山东农业科学,2010(1):78-79.
[2] DONALD C,PORTER I.Integrated control of clubroot[J].J Plant Growth Regul,2009(28):289-303.
[3]孙良菲,任佐华,彭沙莎,等.白菜根肿病菌拮抗微生物的筛选及防治效果评价[J].湖南农业科学,2013(1):84-87.
[4] ARIE T,KOBAYASHI Y,OKADA K,et al.Control of soilborne club-root disease of cruciferous plants by epoxydon from Phoma glomerata[J].Plant Pathology,1998,47:743-748.
[5] CHEAH L H,VEERAKONE S,KENT G.Biological control of clubroot on cauliflower with Trichodermaand Streptomyces spp.[J].New Zealand Plant Protection,2000,53:18-21.
[6]胡靖锋,吴丽艳,林良斌,等.用菌土接种法鉴定云南省主要十字花科作物对根肿病的抗性[J].中国蔬菜,2010(14):71-74.
[7]黄蓉,黄瑞荣,华菊玲,等.小白菜根肿病的接种方法及条件研究[J].中国农学通报,2012,28(31):252-255.
[8]李晓鸥,吴飞燕,钟惠宏,等.大白菜根肿病苗期人工接种抗病性鉴定技术初报[J].中国蔬菜,1990(6):9-10.
[9]李茜,沈向群,耿新翠,等.芸薹根肿菌(Plasmodiophora brassicae)单孢分离接种及生理小种的鉴定[J].植物保护,2012,38(3):95-101.
[10]司军,李成琼,肖崇刚,等.甘蓝根肿病接种方法的研究[J].西南农业大学学报(自然科学版),2003(3):216-219.
[11]王树源,李晏,张毅科.混凝土正交试验结果极差分析与方差分析方法对比[J].建材发展导向,2016,44(12):44-48.
[12]李妍,谢学文,向文胜,等.白菜根肿病的接种方法[J].植物保护学报,2011,38(1):95-96.
[13]唐文华,李晖,孙俊.北京白菜根肿病发生的生态条件及其防治研究[J].北京农业科学,1990(6):6-9.
[14]张淑霞,杨晓云,司朝光,等.大白菜根肿病人工接种鉴定方法比较[J].山东农业科学,2010(1):78-79.