猪源奥斯陆莫拉氏菌的分离鉴定、耐药性及大环内酯耐药基因检测
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摘要
为研究奥斯陆莫拉菌的致病机制及防控措施,对疑似患有奥斯陆莫拉菌的病猪肺组织进行病原分离,得到了1株优势菌命名为ZF2。对分离菌株进行形态学观察、生化试验、分子生物学鉴定,动物回归试验和药敏试验。结果显示,分离菌株为革兰阴性杆菌且对健康仔猪具有较强的致病性,经生化试验和分子生物学分析鉴定为奥斯陆莫拉菌。该分离菌株对阿奇霉素、红霉素呈现较强的耐药性。经大环内酯耐药基因检测结果显示erm B基因为阳性。本研究对奥斯陆莫拉菌病选择合理、正确的抗菌药物提供了一定的科学依据,并为进一步研究其耐药机制奠定了基础。
In order to study the pathogenic mechanism and prevention and control measures of M. serrata,this paper isolated pathogens from the lung tissue of suspected M. serrata,and obtained a dominant strain named ZF2.Morphological observation,16 S r DNA gene sequence analysis,animal regression test and drug sensitivity test were carried out. The results showed that the isolated strain was Gram-negative bacilli and had strong pathogenicity to healthy piglets. It was identified as Moraxella osloensis by biochemical test and molecular biological identification.The strain showed strong resistance to azithromycin and erythromycin. The results of macrolide resistance gene test showed that erm B gene was positive. This study provided a scientific basis for the selection of reasonable and correct antibacterial drugs for molamycin in oslo,and laid a foundation for further research on its resistance mechanism.
In order to study the pathogenic mechanism and prevention and control measures of M. serrata,this paper isolated pathogens from the lung tissue of suspected M. serrata,and obtained a dominant strain named ZF2.Morphological observation,16 S r DNA gene sequence analysis,animal regression test and drug sensitivity test were carried out. The results showed that the isolated strain was Gram-negative bacilli and had strong pathogenicity to healthy piglets. It was identified as Moraxella osloensis by biochemical test and molecular biological identification.The strain showed strong resistance to azithromycin and erythromycin. The results of macrolide resistance gene test showed that erm B gene was positive. This study provided a scientific basis for the selection of reasonable and correct antibacterial drugs for molamycin in oslo,and laid a foundation for further research on its resistance mechanism.
引文
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[14] Sugarman B,Clarridge J. Osteomyelitis caused by Moraxella osloensis[J]. Journal of Clinical Microbiology,1982,15(6):1148-1149.
[15]杜庆玮,孙庆丰,吴金国,等.奥斯陆莫拉菌败血症1例分析[J].现代医药卫生,2009,25(2):258-259.
[16]张京文,邓书杰,姜惠.从脓液、血培养中分离奥斯陆莫拉菌[J].中华医史杂志,1996,19(2):54.
[17]王江博.一株莫拉氏菌新种的分离鉴定与特性研究[D].雅安:四川农业大学,2016.
[18]胡文举,宋艳画,秦佳辰,等.奶牛子宫内膜炎主要致病菌的分离鉴定及药敏试验[J].动物医学进展,2014(5):117-123.
[19]牛晖,徐照学,王二耀,等.奶牛子宫内膜炎血常规分析及病原分离鉴定[J].河南农业科学,2015,44(8):141-144.
[20] Emerson F G,景鲁.奥斯陆莫拉氏菌引起似禽霍乱的病理变化[J].青海畜牧兽医杂志,1984(5):121.
[2] Berger U,Falsen E. Distribution of species of Moraxella and Moraxella-like organisms in the nasopharynx of healthy human adults(author's transl)[J]. Medical Microbiology&Immunology,1976,162(3-4):239.
[3] Alkhatib N J,Younis M H,Alobaidi A S,et al. An unusual osteomyelitis caused by Moraxella osloensis:A case report[J]. International Journal of Surgery Case Reports,2017,41:146-149.
[4]傅响玲,张玉侠,胡晓静,等.新生儿重症监护病房护理信息系统手持终端细菌污染情况的调查研究[J].中国实用护理杂志,2017,33(10):772-774.
[5] Wren M A,Caskey J R,Liu D X,et al. Septic arthritis due to Moraxella osloensis in a rhesus macaque(Macaca mulatta)[J]. Comparative Medicine,2013,63(6):521-527.
[6]张素辉,黄勇富,付利芝,等.山羊源奥斯陆莫拉菌的分子鉴定与分析[J].中国人兽共患病学报,2014,30(1):12-16.
[7]刘玉庆,李璐璐,骆延波,等. EUCAST欧盟药敏试验标准[M].北京:中国标准出版社,2016.
[8] Baron E J,Antonson S. Identification of unusual pathogenic Gram-negative aerobic and facultatively anaerobic bacteria[J]. Clinical Infectious Diseases,1986,24(3):537-537.
[9] Lee W S,Hsueh P R,Yu F L,et al. Moraxella osloensis bacteremia complicating with severe pneumonia in a patient with lung cancer[J]. Journal of Microbiology,Immunology,and Infection,2017,50(3):395-396.
[10] Buchman A L,Pickett M J,Mann L,et al. Central venous catheter infection caused by Moraxella osloensis in a patient receiving home parenteral nutrition[J].Diagnostic Microbiology and Infectious Disease,1993,17(2):163-166.
[11] Shah S S,Ruth A,Coffin S E. Infection due to Moraxella osloensis:case report and review of the literature[J].Clinical Infectious Diseases,2000,30(1):179-181.
[12] Fox-Lewis A,Coltart G,Rice S,et al. Extensive subclinical sinusitis leading to Moraxella osloensis meningitis[J]. IDCases,2016,6:39-42.
[13] Gagnard J C,Hidri N,Grillon A,et al. Moraxella osloensis,an emerging pathogen of endocarditis in immunocompromised patients?[J]. Swiss Medical Weekly,2015,145:w14185.
[14] Sugarman B,Clarridge J. Osteomyelitis caused by Moraxella osloensis[J]. Journal of Clinical Microbiology,1982,15(6):1148-1149.
[15]杜庆玮,孙庆丰,吴金国,等.奥斯陆莫拉菌败血症1例分析[J].现代医药卫生,2009,25(2):258-259.
[16]张京文,邓书杰,姜惠.从脓液、血培养中分离奥斯陆莫拉菌[J].中华医史杂志,1996,19(2):54.
[17]王江博.一株莫拉氏菌新种的分离鉴定与特性研究[D].雅安:四川农业大学,2016.
[18]胡文举,宋艳画,秦佳辰,等.奶牛子宫内膜炎主要致病菌的分离鉴定及药敏试验[J].动物医学进展,2014(5):117-123.
[19]牛晖,徐照学,王二耀,等.奶牛子宫内膜炎血常规分析及病原分离鉴定[J].河南农业科学,2015,44(8):141-144.
[20] Emerson F G,景鲁.奥斯陆莫拉氏菌引起似禽霍乱的病理变化[J].青海畜牧兽医杂志,1984(5):121.