罕见抗-Jk~3合并抗-cE的鉴定
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摘要
目的对罕见的抗-Jk~3合并抗-c E的标本进行鉴定。方法对被检标本分别做ABO血型、Rh血型和Kidd血型鉴定,并作Kidd血型的基因分型;尿素溶血实验以辅助鉴定Jk(a-b-)表型;血清学实验包括自身对照实验、直接抗人球蛋白实验和不规则抗体筛查;因实验室抗体鉴定用Jk(a-b-)表型红细胞为A型,故采用吸收放散实验鉴定标本是否有抗-Jk~。结果被检患者血型为B型,DCCee,自身对照阴性,抗体筛查阳性,直接抗人球蛋白实验阴性;血清学和基因分型鉴定标本kidd血型为Jk(a-b-);尿素溶血实验结果为尿素抵抗。标本检出有抗-Jk~3合并抗-cE抗体。结论对罕见的抗高频抗原抗体,应采用灵活的血清学技术手段并细致分析,以达到鉴定的目的。
Objective To identify a rare sample which has anti-JK~3 combined with anti-cE. Methods The ABO blood group,Rh blood group and Kidd blood group were identified for the tested samples,and the Kidd blood group was genotyped.We performed a urea hemolysis test to assist in identifying the Jk( a-b-) phenotype. Serological experiments including selfcontrol experiments,direct anti-human globulin tests,and irregular antibody screening were conducted. Since the Jk( a-b-)phenotype erythrocytes were type A for laboratory antibody identification,we used the absorption and dispersion test to identify whether the sample had anti-Jk~3. Results The patient' s blood type was type B,DCCee,and self-control was positive.Tthe results of antibody screening were positive and DAT was negative. The kidd blood type of sample was Jk( a-b-) by serological and genotyping tests and urea hemolysis test showed urea resistance. The sample was confirmed to be anti-Jk~3 with anti-cE antibody. Conclusion For rare anti-high-frequency-antigen antibodies,flexible serological techniques should be adopted and analyzed meticulously to achieve the purpose of identification.
Objective To identify a rare sample which has anti-JK~3 combined with anti-cE. Methods The ABO blood group,Rh blood group and Kidd blood group were identified for the tested samples,and the Kidd blood group was genotyped.We performed a urea hemolysis test to assist in identifying the Jk( a-b-) phenotype. Serological experiments including selfcontrol experiments,direct anti-human globulin tests,and irregular antibody screening were conducted. Since the Jk( a-b-)phenotype erythrocytes were type A for laboratory antibody identification,we used the absorption and dispersion test to identify whether the sample had anti-Jk~3. Results The patient' s blood type was type B,DCCee,and self-control was positive.Tthe results of antibody screening were positive and DAT was negative. The kidd blood type of sample was Jk( a-b-) by serological and genotyping tests and urea hemolysis test showed urea resistance. The sample was confirmed to be anti-Jk~3 with anti-cE antibody. Conclusion For rare anti-high-frequency-antigen antibodies,flexible serological techniques should be adopted and analyzed meticulously to achieve the purpose of identification.
引文
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[3] Plau G,Ikin EW,Mourant AE,et al. A new blood group antibody,anti-Jkb. Nature,1953,171(4349):431.
[4] Pinkerton FJ,Mermod LE,Liles BA,et al.The phenotypeJk(a-b-)in the Kidd blood group system.Vox Sang,1959,4(2):155-160.
[5]庄光艳,刘素芳,苗天红,等. 1例罕见的Jk(a-b-)产生抗-Jk3及输血对策.中国输血杂志,2013,26(8):760-762.
[6]肖瑞卿,杨民,隆晓秋. Kidd血型系统Jk(a-b-)血型的调查研究.西南国防医药,2011,21(4):457-458.
[7]朱自严,沈伟,陈和平,等.上海地区部分人群Jk(a-b-)、Dib-、Wrb-、K0、Ena-、Tja-、Ge-稀有血型筛选.中国输血杂志,2002,15(4):232-233.
[8]林国跃,单金晶,张雅楠,等.中国新疆回族人群9种稀有血型系统基因频率调查研究.公共卫生学与预防学,2016,39(8):1026-1030.
[9]洪缨,徐研,王欢,等.成都地区献血人群Jk(a-b-)表型筛查.中国输血杂志,2011,24(4):333-334.
[10]姚洁,梁海燕,陈金美,等.合并抗-E及抗-JKb引起溶血性输血反应1例.检验医学与临床,2016,13(19):2842-2843.
[11]罗广平,汪传喜,姚富柱,等.稀有抗体抗-Jk3的发现及Jk(ab-)血型家系遗传背景研究.中国输血杂志,2008,21(3):172-174.
[2] Allen FH,Diamond LK,Niedziela B. A new blood-group antigen.Nature,1951,167(4247):482.
[3] Plau G,Ikin EW,Mourant AE,et al. A new blood group antibody,anti-Jkb. Nature,1953,171(4349):431.
[4] Pinkerton FJ,Mermod LE,Liles BA,et al.The phenotypeJk(a-b-)in the Kidd blood group system.Vox Sang,1959,4(2):155-160.
[5]庄光艳,刘素芳,苗天红,等. 1例罕见的Jk(a-b-)产生抗-Jk3及输血对策.中国输血杂志,2013,26(8):760-762.
[6]肖瑞卿,杨民,隆晓秋. Kidd血型系统Jk(a-b-)血型的调查研究.西南国防医药,2011,21(4):457-458.
[7]朱自严,沈伟,陈和平,等.上海地区部分人群Jk(a-b-)、Dib-、Wrb-、K0、Ena-、Tja-、Ge-稀有血型筛选.中国输血杂志,2002,15(4):232-233.
[8]林国跃,单金晶,张雅楠,等.中国新疆回族人群9种稀有血型系统基因频率调查研究.公共卫生学与预防学,2016,39(8):1026-1030.
[9]洪缨,徐研,王欢,等.成都地区献血人群Jk(a-b-)表型筛查.中国输血杂志,2011,24(4):333-334.
[10]姚洁,梁海燕,陈金美,等.合并抗-E及抗-JKb引起溶血性输血反应1例.检验医学与临床,2016,13(19):2842-2843.
[11]罗广平,汪传喜,姚富柱,等.稀有抗体抗-Jk3的发现及Jk(ab-)血型家系遗传背景研究.中国输血杂志,2008,21(3):172-174.