摘要
为构建四川核桃良种指纹图谱,分析遗传多样性,增强品种间的区分能力,该研究利用SSR标记技术,对四川29个核桃良种进行遗传多样性和聚类分析。结果表明:(1)11对SSR引物共检测到121个基因型和80个等位基因,平均每个位点7.273个等位基因和11个基因型。(2)11个位点的平均有效等位基因数为3.644,平均观察杂合度为0.645,平均期望杂合度为0.718,平均香农信息指数为1.518,平均多态信息含量为0.680。(3)采用引物组合法利用引物wga001、wga032和zmz02构建了29个核桃品种的指纹图谱。(4)聚类分析结果表明,29个核桃品种按照品种类型优先聚类,四川本地核桃品种聚类关系与地理来源没有明显的相关性。研究认为,选用的11个SSR标记能够较好地运用于四川核桃品种的遗传多样性研究(PIC>0.5);29个四川核桃品种间亲缘关系较近,遗传基础相对较窄。
In this study,we investigated the fingerprint and genetic diversity of Sichuan elite Juglans regia cultivars to improve the identification ability for cultivars.The SSR markers were applied for genetic diversity and cluster analysis of 29 elite J.regiacultivars.The results showed that:(1)121 genotypes and 80 alleles were identified by the 11 SSR markers,and per primer pair has 7.273 alleles and 11 genotypes.(2)Mean effective allele number(Ne),heterozygosity(Ho),expected heterozygosity(He),shannon's index(I)and polymorphic information contents(PIC)of the 11 SSR markers were 3.644,0.645,0.718,1.518 and 0.680,respectively.(3)The fingerprint of 29 J.regiacultivars were constructed by primer combination method using primers wga001,wga032 and zmz02.(4)Cluster analysis showed that the 29 J.regia cultivars were classified according to the priority of variety type.There is no obvious correlation among cluster relationship and geographical origin of the local J.regiacultivars in Sichuan.The results suggested that the 11 SSR markers can be applied to analyze the genetic diversity of walnut varieties in Sichuan(PIC>0.5);the genetic relationship of 29 Sichuan J.regiacultivars was relatively close,and theirs genetic basis are relatively narrow.
引文
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