纳滤膜对鱼鳞小分子胶原肽提取液脱盐性能的研究
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摘要
采用纳滤技术进行鱼鳞小分子胶原肽提取液的脱盐小试实验,研究了不同分子量的纳滤膜对料液脱盐效果及胶原肽回收率的影响。实验结果表明,截留分子量为700 u的卷式纳滤膜能够有效地除去鱼鳞胶原肽提取液中的无机盐类。脱盐后,鱼鳞胶原肽提取液的电导率从26.9 ms/cm下降到80.3μs/cm,无机盐浓度从13.9 g/L下降到0.04 g/L,胶原肽的回收率为72.7%,最终产品的灰分为0.79%。
Nanofiltration technology was applied to the desalination of small-molecule collagen peptide extraction from fish scale. The impact of nanofiltration membranes with different molecular weight cut off( MWCO) on the desalination effect and collagen recovery was researched. The result showed that the optimal membrane was the nanofiltration membrane with MWCO 700 u. After desalination,the conductivity of collagen peptide extraction decreased from 26. 9 ms / cm to 80. 3 μs / cm and the inorganic salt concentration decreased from 13. 9 g / L to 0. 04 g / L. The recovery rate of collagen peptide was 72. 7%. The ash content in the final product was 0. 79%.
Nanofiltration technology was applied to the desalination of small-molecule collagen peptide extraction from fish scale. The impact of nanofiltration membranes with different molecular weight cut off( MWCO) on the desalination effect and collagen recovery was researched. The result showed that the optimal membrane was the nanofiltration membrane with MWCO 700 u. After desalination,the conductivity of collagen peptide extraction decreased from 26. 9 ms / cm to 80. 3 μs / cm and the inorganic salt concentration decreased from 13. 9 g / L to 0. 04 g / L. The recovery rate of collagen peptide was 72. 7%. The ash content in the final product was 0. 79%.
引文
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[20]胡爱军,闫伟,郑捷,等.酶法水解真鲷鱼皮和鱼鳞制取胶原蛋白肽[J].食品工业,2012,33:65-68.
[21]陈日春,熊文飞,蔡一楠,等.鲢鱼鱼鳞胶原蛋白肽抗氧化作用研究[J].食品科学技术学报,2013,31(6):28-35.
[22]宋芹,颜军,郭晓强,等.酶法制取罗非鱼鱼鳞胶原蛋白寡肽的工艺[J].食品研究与开发,2011,32:39-43.
[23]陈晖,易瑞灶,陈俊德,等.膜分离在鱼鳞胶原蛋白中试提取液脱盐过程中的应用[J].食品工业科技,2012,21:210-213.
[24]宋齐,潘凯,曹兵.纳滤膜在乳清脱盐中的应用研究[J].膜科学与技术,2011,31:100-103.
[2]杨立,邹波,李玓瓅,等.鱼鳞抗氧化肽的酶法制备工艺及特性[J].食品科学,2011,32:115-119.
[3]刘小玲,白洋,郑春妍,罗非鱼胶原肽制备中钙回收工艺的研究[J].食品工业科技,2009,8:250-252.
[4]王彦蓉,崔春,赵谋明,等.罗非鱼鱼皮鱼鳞蛋白的酶解及超滤分离[J].食品与发酵工业,2011,37:133-136.
[5]涂宗财,陈剑兵,刘伟,等.酶解鱼鳞胶制备小分子多肽的研究[J].食品科学,2005,26:210-213.
[6]石维焕,许永安,鱼鳞利用的研究进展[J].福建水产,2011,33:67-72.
[7]廉志清,王金,任涛,等.酶法耦合膜技术制备鱼鳞胶原蛋白低聚肽的研究[J].化学与生物工程,2009,26:64-66.
[8]段蕊,张俊杰,杜修桥,鱼鳞组成性质及其加工利用的研究进展[J].食品与机械,2006,22:128-131.
[9]马海乐,王中斌,骆琳,等.不补料连续酶解-膜分离耦合制备鱼鳞胶原蛋白抗氧化肽的研究[J].食品工业科技,2010,8:204-207.
[10]胡松青,张婷婷,郭莎莎,等.鱼鳞明胶ACE抑制肽的制备及其活性研究[J].现代食品科技,2012,28:1491-1494.
[11]周光朝,刘良忠,万菡,等.高水解度草鱼鱼鳞胶原蛋白肽的研究[J].粮食科技与经济,2011,36:52-56.
[12]Pati F,Adhikari B,Dhara S,Isolation and characterization of fish scale collagen of higher thermal stability[J].Bioresource Technology,2009,12(133):1-6.
[13]Ogawa M,Portier R J,Moody M W,et al.Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum(Pogonia cromis)and sheepshead seabream(Archosargus probatocephalus)[J].Food Chemistry,2004(88):495-501.
[14]Wang L,Yang B,Du X,et al.Optimization of conditions for extraction of acid-soluble collagen from grass carp(Ctenopharyngodon idella)by response surface methodology[J].Innovative Food Science and Emerging technologies,2008(9):604-607.
[15]Sankar S,Sekar S,Mohan R,et al.Preparation and partial characterization of collagen sheet from fish(Lates Calcarife)Scales[J].International Journal of Biological Macromolecules,2008(42):6-9.
[16]Kari M,Bhat R.Fish gelatin:properties,challenge,and prospects as an alternative to mammalian gelatins[J].Food Hydrocolloids,2009(23):563
[17]Huo J,Zhao Z.Study on enzymatic hydrolysis of Gadus morrhua skin collagen and molecular weight distribution of hydrolysates[J].Agri-cultural Sciences in China,2009,8(6):723-729.
[18]Bae I,Osatomi K,Yoshida A,et al.Biochemical properties of acid soluble collagens extracted from the skins of under utilised fishes[J].Food Chemistry,2008(108):49-54.
[19]杨莉莉,申锋,熊善柏,等.木瓜蛋白酶制备草鱼鳞胶原肽的工艺优化及产物特性分析[J].食品科技,2012,37:61-65.
[20]胡爱军,闫伟,郑捷,等.酶法水解真鲷鱼皮和鱼鳞制取胶原蛋白肽[J].食品工业,2012,33:65-68.
[21]陈日春,熊文飞,蔡一楠,等.鲢鱼鱼鳞胶原蛋白肽抗氧化作用研究[J].食品科学技术学报,2013,31(6):28-35.
[22]宋芹,颜军,郭晓强,等.酶法制取罗非鱼鱼鳞胶原蛋白寡肽的工艺[J].食品研究与开发,2011,32:39-43.
[23]陈晖,易瑞灶,陈俊德,等.膜分离在鱼鳞胶原蛋白中试提取液脱盐过程中的应用[J].食品工业科技,2012,21:210-213.
[24]宋齐,潘凯,曹兵.纳滤膜在乳清脱盐中的应用研究[J].膜科学与技术,2011,31:100-103.