不同工艺山茶油中生物活性物质含量的比较
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摘要
以不同工艺(冷榨法、热榨法、超临界CO2萃取法、水酶法、浸出法、鲜榨法)山茶油为研究对象,研究了其多酚、黄酮、原花青素、总三萜和木脂素含量。结果表明:冷榨毛油中多酚、黄酮、原花青素、木脂素和总三萜含量高于浸出毛油;超临界CO2萃取山茶油和水酶法山茶油中多酚、黄酮、总三萜和木脂素含量较高;鲜榨山茶油中多酚、黄酮、原花青素、木脂素和总三萜含量分别为271. 6、157. 2、1 400. 0、180. 0 mg/kg和12. 5 mg/g,均远高于其他工艺。鲜榨山茶油是一种高品质食用油,具有广阔的食用和药用价值。
With oil-tea camellia seed oils from different processes( cold pressing,hot pressing,solvent extraction,supercritical CO2 extraction,fresh pressing and aqueous enzymatic extraction) as research objects,the contents of polyphenols,flavonoids,procyaninides,total triterpenoids and lignins were studied. The results showed that the contents of polyphenols,flavonoids,procyaninides,total triterpenoids and lignins in crude cold pressed oil were higher than those in crude solvent extracted oil. The contents of polyphenols,flavonoids,total triterpenoids and lignins in oil-tea camellia seed oils extracted by supercritical CO2 and aqueous enzymatic method were higher. The contents of polyphenols,flavonoids,procyaninides,lignins and total triterpenoids in fresh pressed oil-tea camellia seed oil were 271. 6,157. 2,1 400. 0,180. 0 mg/kg and 12. 5 mg/g,which were far higher than those in the other five processes.The fresh pressed oil-tea camellia seed oil was a kind of high-quality edible oil,and had wide edible and medicinal value.
With oil-tea camellia seed oils from different processes( cold pressing,hot pressing,solvent extraction,supercritical CO2 extraction,fresh pressing and aqueous enzymatic extraction) as research objects,the contents of polyphenols,flavonoids,procyaninides,total triterpenoids and lignins were studied. The results showed that the contents of polyphenols,flavonoids,procyaninides,total triterpenoids and lignins in crude cold pressed oil were higher than those in crude solvent extracted oil. The contents of polyphenols,flavonoids,total triterpenoids and lignins in oil-tea camellia seed oils extracted by supercritical CO2 and aqueous enzymatic method were higher. The contents of polyphenols,flavonoids,procyaninides,lignins and total triterpenoids in fresh pressed oil-tea camellia seed oil were 271. 6,157. 2,1 400. 0,180. 0 mg/kg and 12. 5 mg/g,which were far higher than those in the other five processes.The fresh pressed oil-tea camellia seed oil was a kind of high-quality edible oil,and had wide edible and medicinal value.
引文
[1] SNYDER J M,FRANKEL E N,SELKE E. Capillary gas chromatographic analysis of headspace volatiles from vegetable oils[J]. J Am Oil Chem Soc,1985,62(12):1657-1679.
[2]王萍,张银波,江木兰.多不饱和脂肪酸的研究进展[J].中国油脂,2008,33(12):42-46.
[3]刘世鹏,周伯川.油茶籽的开发利用[J].中国油脂,1996,21(4):39-42.
[4]HELIEH S,JEFFREY O,EBERSOLE L. Green tea polyphenols mediated apoptosis in intestinal epithelial cells by a FADD-dependent pathway[J]. J Cancer Ther,2010,1(3):105-113.
[5]李淑红,李堃,王美.茶多酚对Lewis肺癌的生长抑制、抗氧化及免疫调节作用的研究[J].中国肿瘤生物治疗杂志,2003,10(3):206-209.
[6]PENG Z,XU Z W,WEN W S,et al. Tea polyphenols protect against irradiation-induced injury in submandibular glands’cells:a preliminary study[J]. Arch Oral Biol,2011,56:738-743.
[7]黄华艺,查锡良.黄酮类化合物抗肿瘤研究进展[J].中国新药与临床杂志,2002,21(7):428-433.
[8]BROWNSON D M,AZIOS N G,FUQUA B K,et al. Flavonoid effects relevant to cancer[J]. J Nutr,2002,132(11 Suppl):3482-3489.
[9]GASTRLLO J L,CARRASCO L. Action of 3-methylquercetin on poliovirus RNA replication[J]. J Virol,1987,61(1):3319-3321.
[10]王威.原花青素抑制人结肠癌细胞增殖及分子机制研究[D].广州:暨南大学,2011.
[11]MUTHENNA P,RAGHU G,AKILESHWARI C,et al. Inhibition of protein glycation by procyanidin-B2 enriched fraction of cinnamon:delay of diabetic cataract in rats[J].Iubmb Life,2013,65(11):941-950.
[12]SONG C G,YANG X,MIN L Q,et al. The effect of procyanidin on expression of STAT1 in type 2 diabetes mellitus SD rats with focal cerebral ischemia[J]. Neuro Endocrinol Lett,2014,35(1):68-72.
[13]罗俊,林志彬.灵芝三萜类化合物药理作用研究进展[J].药学学报,2002,37(7):574-578.
[14]El-MEKKAWY S,MESELHY M R,NAKAMURA N,et a1. Anti-HIV-1andanti-HIV-1-protease substances from Ganoder maluciduma[J]. Phytochemistry,1998,49(6):1651-1657.
[15]SERRAINO M,THOMPSON L U. The effect of flaxseed supplementation on early risk markers for mammary carcinogenesis[J]. Cancer Lett,1991,60(2):135-142.
[16]MEAGHER L P,BEECHER G R. Assessment of data on the lignan content of foods[J]. J Food Com Anal,2000,13(6):935-947.
[2]王萍,张银波,江木兰.多不饱和脂肪酸的研究进展[J].中国油脂,2008,33(12):42-46.
[3]刘世鹏,周伯川.油茶籽的开发利用[J].中国油脂,1996,21(4):39-42.
[4]HELIEH S,JEFFREY O,EBERSOLE L. Green tea polyphenols mediated apoptosis in intestinal epithelial cells by a FADD-dependent pathway[J]. J Cancer Ther,2010,1(3):105-113.
[5]李淑红,李堃,王美.茶多酚对Lewis肺癌的生长抑制、抗氧化及免疫调节作用的研究[J].中国肿瘤生物治疗杂志,2003,10(3):206-209.
[6]PENG Z,XU Z W,WEN W S,et al. Tea polyphenols protect against irradiation-induced injury in submandibular glands’cells:a preliminary study[J]. Arch Oral Biol,2011,56:738-743.
[7]黄华艺,查锡良.黄酮类化合物抗肿瘤研究进展[J].中国新药与临床杂志,2002,21(7):428-433.
[8]BROWNSON D M,AZIOS N G,FUQUA B K,et al. Flavonoid effects relevant to cancer[J]. J Nutr,2002,132(11 Suppl):3482-3489.
[9]GASTRLLO J L,CARRASCO L. Action of 3-methylquercetin on poliovirus RNA replication[J]. J Virol,1987,61(1):3319-3321.
[10]王威.原花青素抑制人结肠癌细胞增殖及分子机制研究[D].广州:暨南大学,2011.
[11]MUTHENNA P,RAGHU G,AKILESHWARI C,et al. Inhibition of protein glycation by procyanidin-B2 enriched fraction of cinnamon:delay of diabetic cataract in rats[J].Iubmb Life,2013,65(11):941-950.
[12]SONG C G,YANG X,MIN L Q,et al. The effect of procyanidin on expression of STAT1 in type 2 diabetes mellitus SD rats with focal cerebral ischemia[J]. Neuro Endocrinol Lett,2014,35(1):68-72.
[13]罗俊,林志彬.灵芝三萜类化合物药理作用研究进展[J].药学学报,2002,37(7):574-578.
[14]El-MEKKAWY S,MESELHY M R,NAKAMURA N,et a1. Anti-HIV-1andanti-HIV-1-protease substances from Ganoder maluciduma[J]. Phytochemistry,1998,49(6):1651-1657.
[15]SERRAINO M,THOMPSON L U. The effect of flaxseed supplementation on early risk markers for mammary carcinogenesis[J]. Cancer Lett,1991,60(2):135-142.
[16]MEAGHER L P,BEECHER G R. Assessment of data on the lignan content of foods[J]. J Food Com Anal,2000,13(6):935-947.