12个北美冬青品种的ISSR亲缘关系分析
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摘要
北美冬青Ilex verticillata作为切枝观赏和景观美化植物在中国花卉市场受到越来越多的关注。为给北美冬青新品种选育提供保障,采用简单重复序列间扩增多态性(ISSR)分子标记分析了北美冬青品种间的亲缘关系,从12个ISSR引物中筛选出4个多态性引物;对12个北美冬青品种进行了扩增,共获得63个条带,其中多态性条带占90.48%,所选引物的多态性较高,可用于鉴定北美冬青品种间的相对亲缘关系;12个北美冬青品种间的遗传相似系数为0.54~0.87,亲缘关系相对较近;品种间的遗传相似系数聚类结果表明,各品种间存在不同程度的亲缘关系,其中I.verticillata‘Jim Dandy’和I.verticillata‘Cacapon’间亲缘关系最近。研究结果为北美冬青品种的选育奠定了理论和实践基础。
As an important ornamental and landscape plant, Ilex verticillata has received increasing attention in flower markets of China. To foster breeding of new I. verticillata cultivars, genetic relationships between I. verticillata cultivars were analyzed by inter-simple sequence repeat(ISSR) markers. Four primers with polymorphism among I. verticillata cultivars were screened from 12 candidate ISSR primers. Results revealed a total of63 bands with polymorphic bands accounting for 90.48% implying they could be used to identify relative relationships among different I. verticillata cultivars. The genetic similarity coefficient of the 12 I. verticillata cultivars varied from 0.54 to 0.87 indicating that the genetic relationships among I. verticillata were relatively close.Clustering of these genetic similarity coefficients showed that genetic relationships among cultivars were different with the relationship between I. verticillata ‘Jim Dandy' and I. verticillata ‘Cacapon' being the closest. This study will help lay an important theoretical and practical basis for genetic breeding of I. verticillata.
As an important ornamental and landscape plant, Ilex verticillata has received increasing attention in flower markets of China. To foster breeding of new I. verticillata cultivars, genetic relationships between I. verticillata cultivars were analyzed by inter-simple sequence repeat(ISSR) markers. Four primers with polymorphism among I. verticillata cultivars were screened from 12 candidate ISSR primers. Results revealed a total of63 bands with polymorphic bands accounting for 90.48% implying they could be used to identify relative relationships among different I. verticillata cultivars. The genetic similarity coefficient of the 12 I. verticillata cultivars varied from 0.54 to 0.87 indicating that the genetic relationships among I. verticillata were relatively close.Clustering of these genetic similarity coefficients showed that genetic relationships among cultivars were different with the relationship between I. verticillata ‘Jim Dandy' and I. verticillata ‘Cacapon' being the closest. This study will help lay an important theoretical and practical basis for genetic breeding of I. verticillata.
引文
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[17]林立,王志龙,付涛,等.39个樱花品种亲缘关系的ISSR分析[J].植物研究,2016,36(2):297-304.LIN Li,WANG Zhilong,FU Tao,et al.Genetic relationships of 39 Cerasus cultivars by ISSR analysis[J].Bull Bot Res,2016,36(2):297-304.
[18]付铨盛.毛冬青ISSR指纹图谱的建立及成分动态规律初探[D].广州:广州中医药大学,2016.FU Quansheng.Establish Fingerprint for Ilex pubescens based on ISSR PCR and Preliminary of Ingredients Cumulative[D].Guangzhou:Guangzhou University of Chinese Medicine,2016.
[19]钱永生,王慧中,施农农,等.10种冬青属植物遗传多样性的RAPD和AFLPs分析[J].分子细胞生物学报,2008,41(1):35-43.QIAN Yongsheng,WANG Huizhong,SHI Nongnong,et al.Studies of genetic diversity among 10 species of Ilex based on RAPD and AFLPs[J].J Mol Cell Biol,2008,41(1):35-43.
[20]冷欣,王中生,安树青,等.岛屿特有种全缘冬青遗传多样性的ISSR分析[J].生物多样性,2005,13(6):546-554.LENG Xin,WANG Zhongsheng,AN Shuqing,et al.ISSR analysis of genetic diversity of Ilex integra,an insular endemic plant[J].Biodivers Sci,2005,13(6):546-554.
[2]钱萍,季春峰.美国州树研究[J].江西林业科技,2011(6):26-27.QIAN Ping,JI Chunfeng.Research on state trees of United States.[J].J Jiangxi For Sci Technol,2011(6):26-27.
[3]BAILES C.Hollies for Gardeners[M].Portland:Timber Press,2006:170-173.
[4]赵谦,杜虹,庄东红.ISSR分子标记及其在植物研究中的应用[J].分子植物育种,2007,5(6S):123-129.ZHAO Qian,DU Hong,ZHUANG Donghong.ISSR molecular marker and its application in plant research[J].Mol Plant Breed,2007,5(6S):123-129.
[5]周喜军.冬青属植物ISSR分子标记及枸骨组织培养体系的建立[D].郑州:河南农业大学,2009.ZHOU Xijun.Inter-Simple Sequence Repeat Molecular Markers in Ilex and the Tissue Cculture System of Ilex cornuta Lindl ex Paxt[D].Zhengzhou:Henan Agricultural University,2009.
[6]REDDY M P,SARLA N,SIDDIQ E A.Inter simple sequence repeat(ISSR)polymorphism and its application in plant breeding[J].Euphytica,2002,128(1):9-17.
[7]陈良华,胡庭兴,张帆,等.用AFLP技术分析四川核桃资源的遗传多样性[J].植物生态学报,2008,32(6):1362-1372.CHEN Lianghua,HU Tingxing,ZHANG Fan,et al.Genetic diversity of four Juglans populations revealed by AFLP in Sichuan Province,China[J].J Plant Ecol,2008,32(6):1362-1372.
[8]POTTER D,GAO Fangyou,AIELLO G.Inter-simple sequence repeat markers for fingerprinting and determining genetic relationships of walnut(Juglans regia)cultivars[J].J Amer Soc Hort Sci,2002,127(1):75-81.
[9]王滑,郝俊民,王宝庆,等.中国核桃8个天然居群遗传多样性分析[J].林业科学,2007,43(7):120-124.WANG Hua,HAO Junmin,WANG Baoqing,et al.SSR analysis of genetic diversity of eight natural walnut populations in China[J].Sci Silv Sin,2007,43(7):120-124.
[10]陈良华,胡庭兴,张帆.核桃与铁核桃种间关系的RAPD鉴定[J].四川农业大学学报,2007,25(4):513-516.CHEN Lianghua,HU Tingxing,ZHANG Fan.Identification of relationship between Juglans regia L.and J.sigillata Dode by RAPD markers[J].J Sichuan Agric Univ,2007,25(4):513-516.
[11]陈少瑜,杨恩,张雨,等.云南核桃品种遗传多样性的RAPD和ISSR标记研究[J].河北林果研究,2007,22(1):56-61.CHEN Shaoyu,YANG En,ZHANG Yu,et al.Genetic diversity of Juglans silillata Dode varieties by RAPD and ISSR markers[J].Hebei J For Orchard Res,2007,22(1):56-61.
[12]POLLEGIONI P,BARTOLI S,CANNATA F,et al.Genetic differentiation of four Italian walnut(Juglans regia L.)varieties by inter simple sequence repeat(ISSR)[J].J Genet Breed,2003,57:231-240.
[13]JIANG Zhimin,CHEN Yuxia,BAO Ying.Population genetic structure of Tamarix chinensis in the Yellow River Delta,China[J].Plant Syst Evol,2012,298(1):147-153.
[14]柴晓娟,郭玮龙,陈慧,等.蔺草种质资源遗传多样性的ISSR分析[J].浙江农林大学学报,2017,34(3):552-558.CHAI Xiaojuan,GUO Weilong,CHEN Hui,et al.Genetic diversity of Juncus effuses germplasm by ISSR markers[J].J Zhejiang A&F Univ,2017,34(3):552-558.
[15]董海燕,季孔庶,侯伯鑫,等.基于ISSR标记的红花檵木品种亲缘关系分析[J].园艺学报,2014,41(2):365-374.DONG Haiyan,JI Kongshu,HOU Boxin,et al.Genetic relatives analysis of 41 Loropetalum chinense var.rubrum cultivars by ISSR markers[J].Acta Hortic Sin,2014,41(2):365-374.
[16]田大翠,朱宏波,郭建夫,等.马铃薯遗传多样性的ISSR分析[J].中国马铃薯,2010,24(1):1-5.TIAN Dacui,ZHU Hongbo,GUO Jianfu,et al.Genetic diversity analysis based on potato ISSR[J].Chin Potato J,2010,24(1):1-5.
[17]林立,王志龙,付涛,等.39个樱花品种亲缘关系的ISSR分析[J].植物研究,2016,36(2):297-304.LIN Li,WANG Zhilong,FU Tao,et al.Genetic relationships of 39 Cerasus cultivars by ISSR analysis[J].Bull Bot Res,2016,36(2):297-304.
[18]付铨盛.毛冬青ISSR指纹图谱的建立及成分动态规律初探[D].广州:广州中医药大学,2016.FU Quansheng.Establish Fingerprint for Ilex pubescens based on ISSR PCR and Preliminary of Ingredients Cumulative[D].Guangzhou:Guangzhou University of Chinese Medicine,2016.
[19]钱永生,王慧中,施农农,等.10种冬青属植物遗传多样性的RAPD和AFLPs分析[J].分子细胞生物学报,2008,41(1):35-43.QIAN Yongsheng,WANG Huizhong,SHI Nongnong,et al.Studies of genetic diversity among 10 species of Ilex based on RAPD and AFLPs[J].J Mol Cell Biol,2008,41(1):35-43.
[20]冷欣,王中生,安树青,等.岛屿特有种全缘冬青遗传多样性的ISSR分析[J].生物多样性,2005,13(6):546-554.LENG Xin,WANG Zhongsheng,AN Shuqing,et al.ISSR analysis of genetic diversity of Ilex integra,an insular endemic plant[J].Biodivers Sci,2005,13(6):546-554.