基于灰色关联度法不同产地矿物药白矾 体外抗宫颈癌谱效关系研究
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摘要
目的研究不同产地矿物药白矾中无机元素与抗宫颈癌药效谱效关系,为明确白矾中抗宫颈癌的药效物质基础提供实验依据。方法采用ICP-MS法建立不同产地矿物药白矾中差异元素轮廓谱;利用MTT法测得不同产地矿物药白矾对宫颈癌细胞药效;用灰色关联度法将不同产地矿物药白矾元素轮廓谱与抑制率关联。结果矿物药白矾能够抑制宫颈癌细胞生长,灰色关联度分析显示白矾抑制宫颈癌细胞关联系数≥0.6的差异元素为Cr、Ca、Al、K。结论 Cr、Ca、Al、K元素是白矾能够抑制宫颈癌细胞生长的药效物质基础。
Objective To determine the effect of inorganic elements in alum of different origins on the spectrum-effect relationship of the inhibition Hela cells, and provide experimental basis for determining the active components in the alum of inhibition Hela cells. Methods ICP-MS was used to determine the different elements in the alum of different origins. The inhibition ratios of different elements of alum of different origins to the Hela cells were detected by MTT assay. Then the grey correlational grade between the inhibition ratio and elements was analyzed. Results The grey correlation method showed that Cr, Ca, Al and K were the elements with the correlation coefficient of inhibition rate greater than 0.6. Conclusion Alum inhibits the growth of Hela cells, among which Cr, Ca, Al and K elements are the active components.
Objective To determine the effect of inorganic elements in alum of different origins on the spectrum-effect relationship of the inhibition Hela cells, and provide experimental basis for determining the active components in the alum of inhibition Hela cells. Methods ICP-MS was used to determine the different elements in the alum of different origins. The inhibition ratios of different elements of alum of different origins to the Hela cells were detected by MTT assay. Then the grey correlational grade between the inhibition ratio and elements was analyzed. Results The grey correlation method showed that Cr, Ca, Al and K were the elements with the correlation coefficient of inhibition rate greater than 0.6. Conclusion Alum inhibits the growth of Hela cells, among which Cr, Ca, Al and K elements are the active components.
引文
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[5]李文华.大肠癌防治方药的中医文献研究[J].山东中医药大学学报,2003,27(6):443-447.
[6]陈祥晖,樊佳新,王帅,等.不同产地白矾差异元素的研究[J].中南药学,2016,14(3):310-313.
[7]闻芝梅,陈君石译.现代营养学[M]. 7版.北京:人民卫生出版社,1998:4.
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[9]Holt PR. Dairy foods and prevention of colon cancer:human studies[J]. J Am Coll Nutr,1999,18(5 Suppl):379S-391S.
[10]肖俊勋,张崇盛.微量元素与疾病的关系[J].哈尔滨医药,1991,11(1):59-62.
[11]徐风华,张志萍,高秀云,等.复方硫酸铝注射液体外及小鼠瘤体注射抗肿瘤作用的研究[J].解放军药学学报,2005,21(6):420-422.
[12]康元朝.经膀胱镜注射复方硫酸铝液治疗膀胱肿瘤[J].中国微创外科杂志,2005,5(6):453-455.
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[14]施丽飞,薛大方,郝爱国,等. 3种抗肿瘤中成药中10种人体必需微量元素含量分析[J].中国中医药科技,2007,14(3):81-82.