山东省东方蜜蜂微孢子虫病流行状况及防控建议
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摘要
东方蜜蜂微孢子虫病作为一种慢性传染病,严重影响蜂群的生存,阻碍养蜂产业的发展,进而制约了农业经济的发展。为了解东方蜜蜂微孢子虫病在山东省的流行状况,调研了山东省17个地(市) 73个蜂场,通过检测、实地考察等方式掌握了山东省东方蜜蜂微孢子虫病的流行规律,在此基础上提出了东方蜜蜂微孢子虫病的防治措施。此次调研为山东省东方蜜蜂微孢子虫病防治体系的建立提供了实际依据,为山东省养蜂产业的健康发展奠定了基础。
Microsporidiosis,as a chronic infectious disease,seriously impacts the survival of bee colonies,hinders the development of beekeeping industry,and further restricts the development of agricultural economy.In order to understand the prevalence of microsporidiosis in shandong province,we investigated 73 bee farms in 17 cities of shandong province,obtained the prevalence trend of microsporidiosis in shandong province by detection and field visits.On this basis,the prevention and control measures of microsporidiosis were put forward.This study provides a practical basis to establish the microsporidium pest control system and lay a foundation for the healthy development of beekeeping industry in shandong province.
Microsporidiosis,as a chronic infectious disease,seriously impacts the survival of bee colonies,hinders the development of beekeeping industry,and further restricts the development of agricultural economy.In order to understand the prevalence of microsporidiosis in shandong province,we investigated 73 bee farms in 17 cities of shandong province,obtained the prevalence trend of microsporidiosis in shandong province by detection and field visits.On this basis,the prevention and control measures of microsporidiosis were put forward.This study provides a practical basis to establish the microsporidium pest control system and lay a foundation for the healthy development of beekeeping industry in shandong province.
引文
[1]郑火青,胡福良.蜜蜂——新兴的模式生物[J].昆虫学报,2009,52(2):210-215.
[2]MUNOZ I,CEPERO A,PINTO M A,et al.Presence of Nosema ceranae associated with honeybee queen introduc-tions[J].Infection Genetics&Evolution,2014,23(2):161-168.
[3]李旭涛,周天旭,李文琛.蜜蜂病害防治的基本体系及用药安全[J].甘肃畜牧兽医,2017,47(7):82-86.
[4]刘学录,童金凤,马振刚.蜜蜂主要病害及其病原PCR检测研究进展[J].南方农业学报,2016,47(1):147-152.
[5]MART N-HERN NDEZ et al.2011 Mariano Higes,Raquel Martín-Hernández,Encarna Garrido-Bailón,et al.Honeybee colony collapse due to Nosema ceranae in professional apiaries[J].Environmental Microbiology Reports,2010,1(2):110-113.
[6]汪方炜,鲁兴萌.昆虫的微孢子虫病[J].应用昆虫学报,2003,40(1):5-8.
[7]刘锋,王强,代平礼,等.蜜蜂微孢子虫在中国的自然种系构成初探[J].应用昆虫学报,2008,45(6):963-966.
[8]AUFAUVRE J,BIRON D G,VIDAU C,et al.Parasite-insecticide interactions:a case study of Nosema ceranae and fipronil synergy on honeybee[J].Scientific Reports,2012,2(6):326.
[9]王营.家蚕微孢子虫(Nosema bombycis)功能基因组研究——家蚕微孢子虫孢壁蛋白Nb SWP16和Nb OTU1的鉴定及功能分析[D].西南大学,2015.
[10]黄坚.蜜蜂孢子虫病的防治方法[J].蜜蜂杂志,1997,(9):22-23
[11]王安.蜜蜂病害研究进展[J].中国蜂业,2010,61(1):51-52.
[12]张素贞,何超,王艳丽,等.重庆地区东方蜜蜂微孢子虫的鉴定及分子遗传多样性分析[J].西南农业学报,2015,28(5):2323-2330.
[13]黄文诚.防治蜜蜂微孢子虫病的药剂[J].蜜蜂杂志,2009,29(12):22-25.
[14]颜珣,韩日畴.我国蜜蜂主要病原检测技术[J].应用昆虫学报,2008,45(3):483-488.
[15]HAMIDUZZAMAN M M,GUZMANNOVOA E,GOODWIN P H.Amultiplex PCR assay to diagnose and quantify Nosema infections in honey bees(Apis mellifera)[J].Journal of Invertebrate Pathology,2010,105(2):151-155.
[16]刘楠楠,王志.蜜蜂微孢子虫病感染情况调查与分析[J].蜜蜂杂志,2014,34(4):12-14.
[17]EIRI D M,SUWANNAPONG G,ENDLER M,et al.Nosema ceranae Can Infect Honey Bee Larvae and Reduces Subsequent Adult Longevity[J].2015,10(5):101-108.
[18]吴杰,陈文锋,李继莲.感染蜜蜂及熊蜂的微孢子虫检测与鉴定方法[J].中国农学通报,2010,26(19):8-12.
[19]刘锋.蜜蜂微孢子虫种质资源调查及免疫学诊断技术研究[D].中国农业科学院,2009.
[20]秦浩然,李继莲,和绍禹,等.Calcofluor White M2R与Sytox Green双重染色法鉴别蜜蜂微孢子虫[J].应用昆虫学报,2012,49(5):1392-1396.
[21]CHEN Y,EVANS J D,SMITH I B,et al.Nosema ceranae is′A′Ia long-present and wide-spread microsporidian infection of the European honey bee(Apis mellifera)in the United States[J].Journal of Invertebrate Pathology,2008,97(2):186.
[22]欧阳红燕,刘玉梅,刘彩珍.蜜蜂微孢子虫病研究进展[J].养蜂科技,2002(6):17-19.
[23]许瑛瑛,胡福良,陈大幅,郑火青.蜜蜂孢子虫病的检测与防治研究进展[J].中国蜂业,2018,69(01):64-68.
[24]陈秀贤,蔺哲广,胡福良,等.烟曲霉素在防治蜜蜂孢子虫病中的应用研究进展[J].环境昆虫学报,2016,38(3):648-654.
[2]MUNOZ I,CEPERO A,PINTO M A,et al.Presence of Nosema ceranae associated with honeybee queen introduc-tions[J].Infection Genetics&Evolution,2014,23(2):161-168.
[3]李旭涛,周天旭,李文琛.蜜蜂病害防治的基本体系及用药安全[J].甘肃畜牧兽医,2017,47(7):82-86.
[4]刘学录,童金凤,马振刚.蜜蜂主要病害及其病原PCR检测研究进展[J].南方农业学报,2016,47(1):147-152.
[5]MART N-HERN NDEZ et al.2011 Mariano Higes,Raquel Martín-Hernández,Encarna Garrido-Bailón,et al.Honeybee colony collapse due to Nosema ceranae in professional apiaries[J].Environmental Microbiology Reports,2010,1(2):110-113.
[6]汪方炜,鲁兴萌.昆虫的微孢子虫病[J].应用昆虫学报,2003,40(1):5-8.
[7]刘锋,王强,代平礼,等.蜜蜂微孢子虫在中国的自然种系构成初探[J].应用昆虫学报,2008,45(6):963-966.
[8]AUFAUVRE J,BIRON D G,VIDAU C,et al.Parasite-insecticide interactions:a case study of Nosema ceranae and fipronil synergy on honeybee[J].Scientific Reports,2012,2(6):326.
[9]王营.家蚕微孢子虫(Nosema bombycis)功能基因组研究——家蚕微孢子虫孢壁蛋白Nb SWP16和Nb OTU1的鉴定及功能分析[D].西南大学,2015.
[10]黄坚.蜜蜂孢子虫病的防治方法[J].蜜蜂杂志,1997,(9):22-23
[11]王安.蜜蜂病害研究进展[J].中国蜂业,2010,61(1):51-52.
[12]张素贞,何超,王艳丽,等.重庆地区东方蜜蜂微孢子虫的鉴定及分子遗传多样性分析[J].西南农业学报,2015,28(5):2323-2330.
[13]黄文诚.防治蜜蜂微孢子虫病的药剂[J].蜜蜂杂志,2009,29(12):22-25.
[14]颜珣,韩日畴.我国蜜蜂主要病原检测技术[J].应用昆虫学报,2008,45(3):483-488.
[15]HAMIDUZZAMAN M M,GUZMANNOVOA E,GOODWIN P H.Amultiplex PCR assay to diagnose and quantify Nosema infections in honey bees(Apis mellifera)[J].Journal of Invertebrate Pathology,2010,105(2):151-155.
[16]刘楠楠,王志.蜜蜂微孢子虫病感染情况调查与分析[J].蜜蜂杂志,2014,34(4):12-14.
[17]EIRI D M,SUWANNAPONG G,ENDLER M,et al.Nosema ceranae Can Infect Honey Bee Larvae and Reduces Subsequent Adult Longevity[J].2015,10(5):101-108.
[18]吴杰,陈文锋,李继莲.感染蜜蜂及熊蜂的微孢子虫检测与鉴定方法[J].中国农学通报,2010,26(19):8-12.
[19]刘锋.蜜蜂微孢子虫种质资源调查及免疫学诊断技术研究[D].中国农业科学院,2009.
[20]秦浩然,李继莲,和绍禹,等.Calcofluor White M2R与Sytox Green双重染色法鉴别蜜蜂微孢子虫[J].应用昆虫学报,2012,49(5):1392-1396.
[21]CHEN Y,EVANS J D,SMITH I B,et al.Nosema ceranae is′A′Ia long-present and wide-spread microsporidian infection of the European honey bee(Apis mellifera)in the United States[J].Journal of Invertebrate Pathology,2008,97(2):186.
[22]欧阳红燕,刘玉梅,刘彩珍.蜜蜂微孢子虫病研究进展[J].养蜂科技,2002(6):17-19.
[23]许瑛瑛,胡福良,陈大幅,郑火青.蜜蜂孢子虫病的检测与防治研究进展[J].中国蜂业,2018,69(01):64-68.
[24]陈秀贤,蔺哲广,胡福良,等.烟曲霉素在防治蜜蜂孢子虫病中的应用研究进展[J].环境昆虫学报,2016,38(3):648-654.