核酸适配体在食源性致病菌检测中应用的研究进展
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摘要
核酸适配体(aptamer)是经体外筛选可特异性识别并结合靶分子的寡核苷酸片段(单链DNA/RNA序列),具有高特异性、高灵敏度、高亲和力及易于修饰等优点,已在食源性致病菌检测领域得到应用。本文主要对核酸适配体筛选技术分类以及纳米金适配体技术、荧光适配体技术、电化学适配体技术、流式细胞适配体技术和表面增强拉曼适配体技术在食源性致病菌检测中的应用作简要综述。
The aptamer is an oligonucleotide fragment (single-stranded DNA/RNA sequence) which can specifically recognize and bind to target molecules by screening in vitro. It has the obvious advantages of high specificity and sensitivity,high affinity and simplicity of modification,and has been applied in the field of foodborne pathogenic bacteria detection. The classification of aptamer screening technologies and nanogold aptamer technology,fluorescence aptamer technology,electrochemical aptamer technology,flow cytometry technology and surface enhanced Raman aptamer technology in the detection of foodborne pathogenic bacteria were mainly reviewed.
The aptamer is an oligonucleotide fragment (single-stranded DNA/RNA sequence) which can specifically recognize and bind to target molecules by screening in vitro. It has the obvious advantages of high specificity and sensitivity,high affinity and simplicity of modification,and has been applied in the field of foodborne pathogenic bacteria detection. The classification of aptamer screening technologies and nanogold aptamer technology,fluorescence aptamer technology,electrochemical aptamer technology,flow cytometry technology and surface enhanced Raman aptamer technology in the detection of foodborne pathogenic bacteria were mainly reviewed.
引文
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[34]Kim S E,Su W,Cho M,et al.Harnessing aptamers for electrochemical detection of endotoxin[J].Analytical Biochemistry,2012,424(1):12-20.
[35]Moon J,Kim G,Lee S,et al.Identification of Salmonella typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysis[J].Journal of Microbiological Methods,2013,95(2):162-166.
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[37]Yuan J,Tao Z,Yu Y,et al.A visual detection method for Salmonella typhimurium based on aptamer recognition and nanogold labeling[J].Food Control,2014,37:188-192.
[38]Li H,Chen Q,Ouyang Q,et al.Fabricating a novel Raman spectroscopy-based aptasensor for rapidly sensing Salmonella typhimurium[J].Food Analytical Methods,2017,10(9):3032-3041.
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[41]Duan N,Wu S,Yu Y,et al.A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels[J].Analytica Chimica Acta,2013,804:151-158.
[42]Liu G,Yu X,Xue F,et al.Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8[J].Microchimica Acta,2012,178(1-2):237-244.
[43]杨小娇.体外SELEX技术筛选沙门氏菌O8适体的方法学研究及初步应用[D].合肥:合肥工业大学,2012.
[44]Hyeon J Y,Chon J W,CHOI I S,et al.Development of RNA aptamers for detection of Salmonella enteritidis[J].Journal of Microbiological Methods,2012,89(1):79-82.
[45]Cao X,Li S,Chen L,et al.Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus[J].Nucleic Acids Research,2009,37(14):4621-4628.
[46]Ramlal S,Mondal B,Lavu P S,et al.Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface components[J].International Journal of Food Microbiology,2018,265:74-83.
[47]董晓琳,李志萍,高玮村,等.基于适配体的金黄色葡萄球菌流式细胞术检测方法[J].东北农业科学,2016,41(3):81-86.
[48]Hedayati Ch M,Amani J,Sedighian H,et al.Isolation of a new ssDNA aptamer against Staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatography[J].Journal of Molecular Recognition,2016,29(9):436-445.
[49]Xiong X,Shi X,Liu Y,et al.An aptamer-based electrochemical biosensor for simple and sensitive detection of Staphylococcal enterotoxin B in milk[J].Analytical Methods,2018,10(3):365-370.
[50]Han S R,Lee S W.In vitro selection of RNA aptamer specific to Staphylococcus aureus[J].Annals of Microbiology,2014,64(2):883-885.
[51]Huang Y,Chen X,Duan N,et al.Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1[J].Food Chemistry,2015,166:623-629.
[52]Huang Y,Chen X,Xia Y,et al.Selection,identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A[J].Analytical Methods,2014,6(3):690-697.
[53]Duan N,Wu S,Chen X,et al.Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticus[J].Journal of Agricultural and Food Chemistry,2012,60(16):4034-4038.
[54]Duan N,Yan Y,Wu S,et al.Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scattering[J].Food Control,2016,63:122-127.
[55]Liu G,Lian Y,Gao C,et al.In vitro selection of DNA aptamers and fluorescence-based recognition for rapid detection Listeria monocytogenes[J].Journal of Integrative Agriculture,2014,13(5):1121-1129.
[56]Duan N,Ding X,He L,et al.Selection,identification and application of a DNA aptamer against Listeria monocytogenes[J].Food Control,2013,33(1):239-243.
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[2]The U.S.Department of Agriculture,2018.https://www.fsis.usda.gov/wps/portal/fsis/home.
[3]The U.S.Food and Drug Administration,2018.https://www.fda.gov.
[4]黄欣迪,涂晓波,亓双,等.食源性致病菌的检测方法及其发展趋势[J].食品安全质量检测学报,2016(7):4794-4800.
[5]Teramura H,Sekiguchi J,Inoue K.A novel chromogenic screening medium for isolation of enterohemorrhagic Escherichia coli[J].Biocontrol Science,2013,18:111-115.
[6]Mar?álková K,Purevdorj K,Janová P,et al.Quantitative real-time PCR detection of putrescine-producing gram-negative bacteria[J].Potravinarstvo Slovak Journal of Food Sciences,2017,11(1):355-362.
[7]胡金强,雷俊婷,詹丽娟,等.食源性微生物的分子生物学检测方法的研究进展[J].食品工业,2014,35(7):201-204.
[8]黄韬睿,李玉锋,王鑫.PCR和ELISA在食源性致病菌检测中的应用[J].现代农业科技,2008(9):182-184.
[9]Brandt S M,Paulin S M.Quantifying colonization potential of enterohemorrhagic Escherichia coli O157 using bovine in vitro organ culture and immunofluorescent staining[J].Foodborne Pathogens and Disease,2012,9(12):1064-1070.
[10]鄢雷娜,罗跃华,刘绪平,等.乳制品中常见食源性致病菌检测技术的研究进展[J].食品安全质量检测学报,2017(8):137-141.
[11]Banerjee J,Nilsen-Hamilton M.Aptamers:Multifunctional molecules for biomedical research[J].Journal of Molecular Medicine,2013,91(12):1333-1342.
[12]Ellington A D,Szostak J W.In vitro selection of RNA molecules that bind specific ligands[J].Nature,1990,346(6287):818-822.
[13]Tuerk C,Gold L.Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase[J].Science,1990,249(4968):505-510.
[14]段诺.食源性致病菌适配体的筛选及分析应用研究[D].无锡:江南大学,2013.
[15]Mercier M C,Dontenwill M,Choulier L.Selection of nucleic acid aptamers targeting tumor cell-surface protein biomarkers[J].Cancers,2017,9(6):69.
[16]Wang J,Cui X,Yang L,et al.A real-time control system of gene expression using ligand-bound nucleic acid aptamer for metabolic engineering[J].Metabolic Engineering,2017,42:85-97.
[17]Kawasaki S,Fujita Y,Nagaike T,et al.Synthetic mRNA devices that detect endogenous proteins and distinguish mammalian cells[J].Nucleic Acids Research,2017,45(12):e117.
[18]Macedo B,Cordeiro Y.Unraveling prion protein interactions with aptamers and other prp-binding nucleic acids[J].International Journal of Molecular Sciences,2017,18(5):1023.
[19]Percze K,Szakács Z,Scholz é,et al.Aptamers for respiratory syncytial virus detection[J].Scientific Reports,2017,7:42794.
[20]谭贵良,赵旭,刘妍,等.核酸适配体在食品安全可视化检测中的应用研究进展[J].2016,35(8):2100-2108.
[21]董海燕,王杰,张婷,等.适配体及其在生物医药和诊断分析方面的应用[J].药物分析杂志,2016(3):369-376.
[22]范振符,陈智周,范飞舟,等.新型肿瘤标志物:Mu-GlcNAc[J].标记免疫分析与临床,2014,21(5):514-515.
[23]赵娟,顾月清.核酸适配体在肿瘤诊断与治疗中的研究进展[J].药物生物技术,2013(4):369-372.
[24]卢军利,葛玮东,孙建霞,等.核酸适配体技术在食品有害物检测中的研究进展[J].中国农业科技导报,2015,17(2):151-158.
[25]K?rkk?inen R M,Drasbek M R,McDowall I,et al.Aptamers for safety and quality assurance in the food industry:Detection of pathogens[J].International Journal of Food Science & Technology,2011,46(3):445-454.
[26]Berezovski M,Musheev M,Drabovich A,et al.Non-SELEX selection of aptamers[J].Journal of the American Chemical Society,2006,128(5):1410-1411.
[27]段诺,张田力,吴世嘉,等.肠致病性大肠杆菌适配体筛选研究[J].食品安全质量检测学报,2015(12):4803-4809.
[28]Lee Y J,Han S R,Maeng J S,et al.In vitro selection of Escherichia coli O157∶H7-specific RNA aptamer[J].Biochemical and Biophysical Research Communications,2012,417(1):414-420.
[29]Yu X,Chen F,Wang R,et al.Whole-bacterium SELEX of DNA aptamers for rapid detection of E.coli O157∶H7 using a QCM sensor[J].Journal of Biotechnology,2018,266:39-49.
[30]Amraee M,Oloomi M,Yavari A,et al.DNA aptamer identification and characterization for E.coli O157 detection using cell based SELEX method[J].Analytical Biochemistry,2017,536:36-44.
[31]Wu W,Zhang J,Zheng M,et al.An aptamer-based biosensor for colorimetric detection of Escherichia coli O157∶H7[J].PLoS One,2012,7(11):e48999.
[32]Bruno J G,Carrillo M P,Phillips T.In vitro antibacterial effects of antilipopolysaccharide DNA aptamer-C1qrs complexes[J].Folia Microbiologica,2008,53(4):295-302.
[33]Luo C,Lei Y,Yan L,et al.A rapid and sensitive aptamer-based electrochemical biosensor for direct detection of Escherichia coli O111[J].Electroanalysis,2012,24(5):1186-1191.
[34]Kim S E,Su W,Cho M,et al.Harnessing aptamers for electrochemical detection of endotoxin[J].Analytical Biochemistry,2012,424(1):12-20.
[35]Moon J,Kim G,Lee S,et al.Identification of Salmonella typhimurium-specific DNA aptamers developed using whole-cell SELEX and FACS analysis[J].Journal of Microbiological Methods,2013,95(2):162-166.
[36]Lavu P S,Mondal B,Ramlal S,et al.Selection and characterization of aptamers using a modified whole-Cell bacterium SELEX for the detection of Salmonella enterica serovar typhimurium[J].ACS Combinatorial Science,2016,18(6):292-301.
[37]Yuan J,Tao Z,Yu Y,et al.A visual detection method for Salmonella typhimurium based on aptamer recognition and nanogold labeling[J].Food Control,2014,37:188-192.
[38]Li H,Chen Q,Ouyang Q,et al.Fabricating a novel Raman spectroscopy-based aptasensor for rapidly sensing Salmonella typhimurium[J].Food Analytical Methods,2017,10(9):3032-3041.
[39]Joshi R,Janagama H,Dwivedi H P,et al.Selection,characterization,and application of DNA aptamers for the capture and detection of Salmonella enterica serovars[J].Molecular and Cellular Probes,2009,23(1):20-28.
[40]Ma X,Jiang Y,Jia F,et al.An aptamer-based electrochemical biosensor for the detection of Salmonella[J].Journal of Microbiological Methods,2014,98:94-98.
[41]Duan N,Wu S,Yu Y,et al.A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels[J].Analytica Chimica Acta,2013,804:151-158.
[42]Liu G,Yu X,Xue F,et al.Screening and preliminary application of a DNA aptamer for rapid detection of Salmonella O8[J].Microchimica Acta,2012,178(1-2):237-244.
[43]杨小娇.体外SELEX技术筛选沙门氏菌O8适体的方法学研究及初步应用[D].合肥:合肥工业大学,2012.
[44]Hyeon J Y,Chon J W,CHOI I S,et al.Development of RNA aptamers for detection of Salmonella enteritidis[J].Journal of Microbiological Methods,2012,89(1):79-82.
[45]Cao X,Li S,Chen L,et al.Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus[J].Nucleic Acids Research,2009,37(14):4621-4628.
[46]Ramlal S,Mondal B,Lavu P S,et al.Capture and detection of Staphylococcus aureus with dual labeled aptamers to cell surface components[J].International Journal of Food Microbiology,2018,265:74-83.
[47]董晓琳,李志萍,高玮村,等.基于适配体的金黄色葡萄球菌流式细胞术检测方法[J].东北农业科学,2016,41(3):81-86.
[48]Hedayati Ch M,Amani J,Sedighian H,et al.Isolation of a new ssDNA aptamer against Staphylococcal enterotoxin B based on CNBr-activated sepharose-4B affinity chromatography[J].Journal of Molecular Recognition,2016,29(9):436-445.
[49]Xiong X,Shi X,Liu Y,et al.An aptamer-based electrochemical biosensor for simple and sensitive detection of Staphylococcal enterotoxin B in milk[J].Analytical Methods,2018,10(3):365-370.
[50]Han S R,Lee S W.In vitro selection of RNA aptamer specific to Staphylococcus aureus[J].Annals of Microbiology,2014,64(2):883-885.
[51]Huang Y,Chen X,Duan N,et al.Selection and characterization of DNA aptamers against Staphylococcus aureus enterotoxin C1[J].Food Chemistry,2015,166:623-629.
[52]Huang Y,Chen X,Xia Y,et al.Selection,identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A[J].Analytical Methods,2014,6(3):690-697.
[53]Duan N,Wu S,Chen X,et al.Selection and identification of a DNA aptamer targeted to Vibrio parahemolyticus[J].Journal of Agricultural and Food Chemistry,2012,60(16):4034-4038.
[54]Duan N,Yan Y,Wu S,et al.Vibrio parahaemolyticus detection aptasensor using surface-enhanced Raman scattering[J].Food Control,2016,63:122-127.
[55]Liu G,Lian Y,Gao C,et al.In vitro selection of DNA aptamers and fluorescence-based recognition for rapid detection Listeria monocytogenes[J].Journal of Integrative Agriculture,2014,13(5):1121-1129.
[56]Duan N,Ding X,He L,et al.Selection,identification and application of a DNA aptamer against Listeria monocytogenes[J].Food Control,2013,33(1):239-243.
[57]Fischer C,Hünniger T,Jarck J H,et al.Food sensing:Aptamer-based trapping of Bacillus cereus spores with specific detection via real time PCR in milk[J].Journal of Agricultural and Food Chemistry,2015,63(36):8050-8057.
[58]Duan N,Ding X,Wu S,et al.In vitro selection of a DNA aptamer targeted against Shigella dysenteriae[J].Journal of Microbiological Methods,2013,94(3):170-174.
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