JNK1在2型糖尿病大鼠股动脉纤维病变中的表达及中药桃仁干预研究
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摘要
[目的]观察2型糖尿病大鼠下肢股动脉血管中c-Jun氨基末端激酶(JNK1)的表达及中药桃仁的干预作用。[方法]雄性SD大鼠50只,随机分组为:空白对照组、模型对照组、早期干预组、桃仁高剂量组、桃仁低剂量组各10只;造模成功后,实验组予药物连续干预3周,取材进行实验检测,观察各项指标表达情况。[结果] 1)模型组JNK1的表达较空白对照组明显升高;2)中药桃仁可有效降低JNK1表达,可明显改善糖尿病大鼠股动脉病变情况,减缓纤维化进程,以早期干预组效果最为明显,桃仁高剂量组效果良好,桃仁低剂量组效果欠佳。[结论] 1)糖尿病股动脉病变可能与JNK1的表达、JNK1磷酸化及JNK1基因的表达相关;2)中药桃仁对糖尿病股动脉病变有明显改善作用,可有效预防并抑制股动脉病变的发展进程。
[Objective] Through the experimental examination Ⅱ diabetes rats JNK1 in lower extremity artery blood vessels(c-Jun Nterninal kinase,c-Jun amino terminal kinase) expression and the intervention of traditional Chinese medicine peach kernel function.[Methods] The 50 male Sprague-Dawley rats were randomly divided into blank control group,model control group,early intervention group,high-dose group and low-dose group. After successful modeling,the experimental group was given continuous drug intervention for3 weeks,and samples were collected for experimental testing to observe the expression of various indicators. [Results] 1) The expression of JNK1 in the model group was significantly higher than that in the blank control group;2) peach kernel,a traditional Chinese medicine,can effectively reduce the expression of JNK1,significantly improve the pathological changes in the femoral artery of diabetic rats,and slow down the fibrosis process. The early intervention group has the most obvious effect,the high dose group has a good effect,and the low dose group has a poor effect. [Conclusion] 1) Diabetic femoral artery lesions may be related to JNK1 expression,JNK1 phosphorylation and JNK1 gene expression. 2) Peach kernel,a traditional Chinese medicine,can significantly improve diabetic femoral artery disease,effectively prevent and inhibit the development of femoral artery disease.
[Objective] Through the experimental examination Ⅱ diabetes rats JNK1 in lower extremity artery blood vessels(c-Jun Nterninal kinase,c-Jun amino terminal kinase) expression and the intervention of traditional Chinese medicine peach kernel function.[Methods] The 50 male Sprague-Dawley rats were randomly divided into blank control group,model control group,early intervention group,high-dose group and low-dose group. After successful modeling,the experimental group was given continuous drug intervention for3 weeks,and samples were collected for experimental testing to observe the expression of various indicators. [Results] 1) The expression of JNK1 in the model group was significantly higher than that in the blank control group;2) peach kernel,a traditional Chinese medicine,can effectively reduce the expression of JNK1,significantly improve the pathological changes in the femoral artery of diabetic rats,and slow down the fibrosis process. The early intervention group has the most obvious effect,the high dose group has a good effect,and the low dose group has a poor effect. [Conclusion] 1) Diabetic femoral artery lesions may be related to JNK1 expression,JNK1 phosphorylation and JNK1 gene expression. 2) Peach kernel,a traditional Chinese medicine,can significantly improve diabetic femoral artery disease,effectively prevent and inhibit the development of femoral artery disease.
引文
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[14]徐列明,刘平,刘成,等.桃仁提取物抗实验性肝纤维化的作用观察[J].中国中药杂志,1994,19(8):491-494.
[15]以敏,徐君毅,邓家刚,等.桃仁提取物对寒热不同血瘀证大鼠内皮细胞的损伤及NF-κB表达的影响[J].广西中医药,2017,40(2):65-69.
[2]唐英,吴晓牧,胡凡,等.单纯高血糖诱发大鼠动脉粥样硬化的实验研究[J].中西医结合心脑血管病杂志,2015,13(3):282-284.
[3]王军,徐阳,袁向科,等.实验性糖尿病鼠大血管病变TGF-β1和CTGF的表达及中药的干预作用[J].天津中医药,2012,29(3):266-269.
[4]丁志明,武海阔,王军.胶原蛋白Ⅰ、胶原蛋白Ⅲ在糖尿病大鼠血管病变中的表达及中药的干预[J].天津中医药,2012,29(6):573-575.
[5]徐阳,王军,武海阔.桃核承气汤对糖尿病大鼠大血管纤维病变Toll样受体通路作用研究[J].天津中医药,2016,33(5):299-302.
[6]Yu Y,Lyons TJ.Alethal tetrad in diabetes:hyperglycemia,dyslipidemia,oxidative stress,and endothelial dysfunction[J].Am JMed Sci,2005,330(5):227-232.
[7]Prudenie S,Searpelli D,Chandalia M,et al.The TRIB3 Q84Rpolymorphism and risk of early-onset type2 diabetes[J].J Clin Endoerinol Metab,2009,94(1):190-196.
[8]胡月媛,刘学军,杜毓锋,等.JNK信号通路对大鼠肺纤维化Ι型胶原的影响[J].中国药物与临床,2012,12(3):306-307.
[9]刘旭峰,吴伟,刘晓亮,等.JNK1信号分子参与调控糖尿病大鼠心肌细胞肥大和纤维化[J].现代生物医学进展,2017,3(31):6018-6023.
[10]以敏,邓家刚,郝二伟,等.桃仁对血液循环障碍大鼠内皮细胞凋亡及相关蛋白表达的影响[J].中国实验方剂学杂志,2013,19(14):178-182.
[11]郝二伟,邓家刚,杜正彩,等.平性药桃仁双向适用药性特征的研究[J].中药药理与临床,2011,27(1):56-58.
[12]Liu L,Shi G P.CD31:beyond a marker for endothelial cells[J].Cardiovascular Research,2012,94(1):3.
[13]以敏,邓家刚,郝二伟,等.桃仁提取物对不同病因所致大鼠血液循环障碍的影响[J].中草药,2013,44(7):858-862.
[14]徐列明,刘平,刘成,等.桃仁提取物抗实验性肝纤维化的作用观察[J].中国中药杂志,1994,19(8):491-494.
[15]以敏,徐君毅,邓家刚,等.桃仁提取物对寒热不同血瘀证大鼠内皮细胞的损伤及NF-κB表达的影响[J].广西中医药,2017,40(2):65-69.