黔南州茶园土壤优势菌株浅蓝灰曲霉的分离、纯化及鉴定
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摘要
为生物有机肥的研究开发及利用提供参考,以黔南州茶园土壤为研究材料,采用微生物的分离纯化、形态鉴定和ITS序列分析方法对土壤中的优势菌株进行分离、纯化和鉴定。结果表明:土样中分离、纯化得到优势菌株S4,根据该菌株的菌落和孢子形态等特征,初步推断其为灰曲霉;提取菌株S4的DNA,经ITS序列分析,进一步确定其为浅蓝灰曲霉(Aspergillus Caesiellus)。
The dominant strains in soil of tea plantation was separated,purified and identified by the microbial method and ITS sequence analysis to provide a reference for development and utilization of biological organic fertilizer.Result:The dominant strain S4 separated from soil samples is purified and preliminarily identified as Aspergillus fumigatus according to its characteristics of colony and spore morphology.The S4 strain is further identified as Aspergillus caesiellus after ITS sequence analysis.
The dominant strains in soil of tea plantation was separated,purified and identified by the microbial method and ITS sequence analysis to provide a reference for development and utilization of biological organic fertilizer.Result:The dominant strain S4 separated from soil samples is purified and preliminarily identified as Aspergillus fumigatus according to its characteristics of colony and spore morphology.The S4 strain is further identified as Aspergillus caesiellus after ITS sequence analysis.
引文
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[2]刘红,张清海,林昌虎.贵州主要茶区土壤重金属含量比较与评价[J].中国农学通报,2014(4):210-214.
[3]唐颢,唐劲驰,黎健龙.新型茶园专用有机肥改良土壤酸化的综合效果[J].广东农业科学,2013(12):57-59.
[4] WATANABE K,SAKAI J,HAYANO K.Bacterial extracellular protease activities in field soils under different fertilizer managements[J].Can J Microbiol,2003,49(5):305-312.
[5] CAI Y,LIAO Z,ZHANG J,et al.Effect of ecological organic fertilizer on tomato bacterial wilt and soil microbial diversities[J].Ying Yong Sheng Tai Xue Bao,2003,14(3):349-353.
[6]李磊.不同肥料处理对茶树生长和茶叶品质的影响[D].泰安:山东农业大学,2010.
[7]沈星荣.有机肥料对茶树生长、茶叶品质及经济效益的影响[D].北京:中国农业科学院,2014.
[8]王利民,李卫华,范平,等.长期培肥下红黄壤区茶园土壤酶活性的变化[J].茶叶科学,2012(4):347-352.
[9] OGILVIE L A,HIRSCH P R,JOHNSTON A W.Bacterial diversity of the broadbalk'classical'winter wheat experiment in relation to long-term fertilizer inputs[J].Microb Ecol,2008,56(3):525-537.
[10] WU C S.Controlled release evaluation of bacterial fertilizer using polymer composites as matrix[J].J Control Release,2008,132(1):42-48.
[11]常单娜.我国主要绿肥种植体系中土壤可溶性有机物特性研究[D].北京:中国农业科学院,2015.
[12]唐颢,唐劲驰,方华春.茶树型有机肥的土壤养分及促产提质效应[J].广东农业科学,2015(23):91-95.
[13]张惠.典型绿茶茶园土壤养分和重金属的空间变异特性分析及肥力质量评价[D].北京:中国农业科学院,2015.
[14]李继福.秸秆还田供钾效果与调控土壤供钾的机制研究[D].武汉:华中农业大学,2015.
[15]孔樟良,谢国雄.杭州市典型茶园土壤与茶叶中重金属的积累与来源分析[J].中国农学通报,2015(10):226-231.
[16]林芳,钟灼仔,陈绍康.茶园施用新型缓释复合肥效果比较试验[J].南方农业,2015(3):36-39.
[17]周才碧,陈文品,穆瑞禄,等.普洱茶优势菌株产黄青霉的功能及安全性研究[J].广东农业科学,2015,42(6):21-26.