茶园土壤中优势菌株毛霉菌的分离、纯化及鉴定
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摘要
通过对黔南州茶园中优质土壤进行稀释、培养,分离、纯化得到一株优势菌株,利用形态学和分子生物学方法鉴定该优势菌为毛霉菌。毛霉菌对农业秸秆的腐化和土壤有机物的分解有很大的作用,可以促进茶树的生长发育,有利于茶园生物有机肥的研发。
A dominant strain was isolated and purified by diluting and culturing high-quality soil in tea garden of Qiannan prefecture. The dominant strain was identified as mucor by morphological and molecular biological identification. Mucor plays an important role in the decomposition of agricultural straw and the decomposition of soil organic matter. It can promote the growth and development of tea plants and is conducive to the research and development of bio-organic fertilizers in tea plantations.
A dominant strain was isolated and purified by diluting and culturing high-quality soil in tea garden of Qiannan prefecture. The dominant strain was identified as mucor by morphological and molecular biological identification. Mucor plays an important role in the decomposition of agricultural straw and the decomposition of soil organic matter. It can promote the growth and development of tea plants and is conducive to the research and development of bio-organic fertilizers in tea plantations.
引文
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[17]周才碧,周才富,周小露,等.黔南州茶园土壤优势菌株浅蓝灰曲霉的分离、纯化及鉴定[J].贵州农业科学,2018,46(9):15-18.
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[19]杨光辉,杨善岩,裘娟萍,等.响应面法优化毛霉菌发酵培养基[J].工业微生物,2012,42(1):52-58.
[20]张卫国.一株毛霉菌产脂肪酶特性及发酵条件研究[J].中国酿造,2011(7):134-137.
[21]卓梅芳.卷枝毛霉菌T19不对称还原芳香酮制备手性化合物的研究及其分析方法的建立[D].南宁:广西大学,2012.
[2]刘红,张清海,林昌虎.贵州主要茶区土壤重金属含量比较与评价[J].中国农学通报,2014(4):210-214.
[3]唐颢,唐劲驰,黎健龙.新型茶园专用有机肥改良土壤酸化的综合效果[J].广东农业科学,2013(12):57-59.
[4]WATANABE K,SAKAI J,HAYANO K. Bacterial extracellular protease activities in field soils under different fertilizer managements[J].Can J Microbiol,2003,49(5):305-312.
[5]蔡燕飞,廖宗文,章家恩,等.生态有机肥对番茄青枯病及土壤微生物多样性的影响[J].应用生态学报,2003,14(3):349-353.
[6]李磊.不同肥料处理对茶树生长和茶叶品质的影响[D].泰安:山东农业大学,2010.
[7]沈星荣.有机肥料对茶树生长、茶叶品质及经济效益的影响[D].北京:中国农业科学院,2014.
[8]王利民,李卫华,范平,等.长期培肥下红黄壤区茶园土壤酶活性的变化[J].茶叶科学,2012(4):347-352.
[9] OGILVIE L A,HIRSCH P R,JOHNSTON A W. Bacterial diversity of the broadbalk'classical'winter wheat experiment in relation to long-term fertilizer inputs[J]. Microb Ecol,2008,56(3):525-537.
[10] WU C S. Controlled release evaluation of bacterial fertilizer using polymer composites as matrix[J]. J Control Release,2008,132(1):42-48.
[11]常单娜.我国主要绿肥种植体系中土壤可溶性有机物特性研究[D].北京:中国农业科学院,2015.
[12]唐颢,唐劲驰,方华春.茶树型有机肥的土壤养分及促产提质效应[J].广东农业科学,2015(23):91-95.
[13]张惠.典型绿茶茶园土壤养分和重金属的空间变异特性分析及肥力质量评价[D].北京:中国农业科学院,2015.
[14]李继福.秸秆还田供钾效果与调控土壤供钾的机制研究[D].武汉:华中农业大学,2015.
[15]孔樟良,谢国雄.杭州市典型茶园土壤与茶叶中重金属的积累与来源分析[J].中国农学通报,2015(10):226-231.
[16]林芳,钟灼仔,陈绍康.茶园施用新型缓释复合肥效果比较试验[J].南方农业,2015(3):36-39.
[17]周才碧,周才富,周小露,等.黔南州茶园土壤优势菌株浅蓝灰曲霉的分离、纯化及鉴定[J].贵州农业科学,2018,46(9):15-18.
[18]周小露,周才富,刘丽明,等.黔南州茶园土壤中优势菌株变幻青霉分离及鉴定[J].福建茶叶,2018,40(11):10-11.
[19]杨光辉,杨善岩,裘娟萍,等.响应面法优化毛霉菌发酵培养基[J].工业微生物,2012,42(1):52-58.
[20]张卫国.一株毛霉菌产脂肪酶特性及发酵条件研究[J].中国酿造,2011(7):134-137.
[21]卓梅芳.卷枝毛霉菌T19不对称还原芳香酮制备手性化合物的研究及其分析方法的建立[D].南宁:广西大学,2012.