复方桉芩脂质体鼻喷雾剂对变应性鼻炎豚鼠疗效观察
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摘要
目的:观察复方桉芩脂质体鼻喷雾剂对变应性鼻炎豚鼠的症状及其血清中白介素4(Interleukin-4,IL-4)含量的影响,为新药研发及其临床应用提供实验依据。方法:选用成年Hartley豚鼠40只,雌雄各半,随机分为治疗组(A组)和对照组(B组)两组,每组各20只。两组均采用卵清蛋白雾化吸入+冷水浸足激发方法造模。造模成功后,A组采用复方桉芩脂质体鼻喷雾剂10 mL超声雾化吸入治疗5 min,每日2次,连续2周;B组采用等量生理盐水雾化吸入,操作方法与A组一致。通过比较治疗前后豚鼠症状评分及血清IL-4含量的变化共同评估该鼻喷雾剂的疗效。结果:(1)治疗1周后,两组豚鼠症状评分分别为A组(5.44±1.15)分、B组(6.35±1.22)分;治疗2周后,两组豚鼠症状评分分别为A组(3.67±1.24)分、B组(5.12±1.17)分。治疗1周及2周后两组豚鼠症状评分组间比较,A组均低于B组,差异均具有统计学意义(P<0.05)。(2)治疗前,两组豚鼠血清中IL-4含量分别为A组(35.98±2.73)pg/mL、B组(36.85±2.71)pg/mL,两组比较差异无统计学意义(P>0.05);治疗2周后,两组豚鼠血清中IL-4含量分别为A组(30.24±2.83)pg/mL、B组(34.04±2.75)pg/mL,两组豚鼠血清中IL-4含量比较,A组亦低于B组,差异具有统计学意义(P<0.05)。结论:复方桉芩脂质体鼻喷雾剂对AR豚鼠模型的治疗有效。
Objective:To observe the effects of compound Anqin liposome nasal spray on allergic rhinitis in guinea pigs and their serum levels of interleukin 4(IL-4)to provide experimental evidence for the development and clinical application of new drugs. Methods:40 adult Hartley guinea pigs,each male and female,were selected and randomly divided into treatment group(group A)and control group(group B). Both groups were moulded by ovalbumin through atomization inhalation and foot stimulation by cold water immersion. After the models were established successfully,group A received 10 mL of compound Anqin liposome nasal spray for 5 min,2 times per day for 2 weeks. Normal saline atomization inhalation was used in group B,and the operation method was consistent with group A. The curative effect of the nasal spray was evaluated by both the change of the symptom scores of guinea pigs and the contents of IL-4 before and after treatment. Results:(1)After 1 week of treatment,the symptom scores of the two groups of guinea pigs were respectively:group A(5.44±1.15)points and group B(6.35±1.22)points. After 2 weeks of treatment,the symptom scores of the two groups were respectively:group A(3.67±1.24)points and group B(5.12±1.17)points. After 1 week and 2 weeks of treatment,the symptoms of guinea pigs were compared between the two groups,and the difference was statistically significant(P<0.05).(2)Before treatment,the serum IL-4 contents in the two groups of guinea pigs was(35.98±2.73) pg/mL in group A and(36.85±2.71)pg/mL in group B(P>0.05). After 2 weeks of treatment,the serum IL-4 contents in the two groups of guinea pigs was respectively(30.24±2.83)pg/mL and(34.04±2.75)pg/mL,and the serum IL-4 contents in the group A were also lower than those in the group B(P<0.05). Conclusion:Compound Anqinliposome nasal spray is effective in treating AR guinea pig models.
Objective:To observe the effects of compound Anqin liposome nasal spray on allergic rhinitis in guinea pigs and their serum levels of interleukin 4(IL-4)to provide experimental evidence for the development and clinical application of new drugs. Methods:40 adult Hartley guinea pigs,each male and female,were selected and randomly divided into treatment group(group A)and control group(group B). Both groups were moulded by ovalbumin through atomization inhalation and foot stimulation by cold water immersion. After the models were established successfully,group A received 10 mL of compound Anqin liposome nasal spray for 5 min,2 times per day for 2 weeks. Normal saline atomization inhalation was used in group B,and the operation method was consistent with group A. The curative effect of the nasal spray was evaluated by both the change of the symptom scores of guinea pigs and the contents of IL-4 before and after treatment. Results:(1)After 1 week of treatment,the symptom scores of the two groups of guinea pigs were respectively:group A(5.44±1.15)points and group B(6.35±1.22)points. After 2 weeks of treatment,the symptom scores of the two groups were respectively:group A(3.67±1.24)points and group B(5.12±1.17)points. After 1 week and 2 weeks of treatment,the symptoms of guinea pigs were compared between the two groups,and the difference was statistically significant(P<0.05).(2)Before treatment,the serum IL-4 contents in the two groups of guinea pigs was(35.98±2.73) pg/mL in group A and(36.85±2.71)pg/mL in group B(P>0.05). After 2 weeks of treatment,the serum IL-4 contents in the two groups of guinea pigs was respectively(30.24±2.83)pg/mL and(34.04±2.75)pg/mL,and the serum IL-4 contents in the group A were also lower than those in the group B(P<0.05). Conclusion:Compound Anqinliposome nasal spray is effective in treating AR guinea pig models.
引文
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[13]唐锋,梁少瑜,田元新,等.细辛挥发油抗过敏性鼻炎有效成分及靶点预测的研究[J].中国实验方剂学杂志,2015,21(24):126-131.
[2]安家驹,包文滁,王伯英,等.实用精细化工辞典[M].北京:中国轻工业出版社,2000:833.
[3]王长青,赵长青.冷束缚应激对大鼠变应性鼻炎血清中白细胞介素-4的影响[J].山西医药杂志,2009,38(4):330-331.
[4]赵浩如.一种复方草药胶囊的含量测定以及与标准汤剂的制备比较(英文)[J].中成药,2002(12):19-22.
[5]潘九英,宋世珍,韦亚芳,等.鼻窦3号糖浆的主要成分黄芩苷的含量测定[J].南京中医药大学学报,1999(4):50-51.
[6]王毅.黄芩中黄芩黄素对人皮肤成纤维细胞分泌的嗜酸性粒细胞趋化因子的抑制作用[J].国外医学:中医中药分册,2002(1):26.
[7]吴梅,闫慧,张春梅.黄芩苷作为一种抗生物制剂的过敏反应的研究[J].微生物学免疫学进展,2007(4):48-50.
[8]明彩荣,王晓男.现代医学技术观察中药抗过敏反应机理的实验研究[J].中医药学刊,2006(5):940-941.
[9]曾光,梁清华,游万辉,等.黄芩苷对T淋巴细胞增殖与活化的影响[J].中药药理与临床,2007(6):20-22.
[10]吕小琴,唐法娣,王砚,等.蓝桉油对脂多糖引起的大鼠慢性支气管炎及黏蛋白高分泌的影响[J].中国中药杂志,2004(2):77-80.
[11]詹瑾,耿维凤,鄢学芬.桉柠蒎的药理作用与临床评价[J].中国现代药物应用,2008(24):26-28.
[12]陶翠.油樟叶提取物的抗菌、镇痛和抗炎活性及其作用机理研究[D].成都:四川农业大学,2011.
[13]唐锋,梁少瑜,田元新,等.细辛挥发油抗过敏性鼻炎有效成分及靶点预测的研究[J].中国实验方剂学杂志,2015,21(24):126-131.