针刺对类风湿性关节炎小鼠血清IL-4和TNF-α的影响
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摘要
目的:观察针刺治疗对类风湿性关节炎小鼠血清白介素-4(IL-4)和肿瘤坏死因子-α(TNF-α)的影响,进一步探讨针刺治疗该病的作用机制。方法:取小鼠24只,随机分为空白组、风湿性关节炎模型组、针刺组,每组8只。模型组、针刺组小鼠建立类风湿性关节炎模型,方法分别为于小鼠双后足跖的底部皮内注射50μL不完全弗氏佐剂。针刺组取小鼠双侧肾俞、足三里穴进行针刺治疗,每天1次,每周6次,针刺3周,疗程之中间休息1天。采用ELISA法检测小鼠血清中IL-4和TNF-α含量。结果:与空白组相比,模型组小鼠血清中IL-4含量降低,TNF-α含量升高,差异极显著(P<0.01);与模型组小鼠相比,针刺组小鼠血清中IL-4含量升高,TNF-α含量降低,差异显著(P<0.05)。结论:针刺可降低类风湿性关节炎大鼠血清TNF-α含量,提高IL-4含量,调节Th1/Th2细胞因子失衡状态,对于临床控制类风湿性关节炎的病情发展有积极作用。
Objective:To observe the effect of acupuncture on serum interleukin-4(IL-4)and tumor necrosis factor-α(TNF-α)in mice with rheumatoid arthritis and to explore the mechanism of the effect of acupuncture on rheumatoid arthritis(RA).Methods:Twenty-four male mice were randomly divided into three groups,including blank group,RA model group and acupuncture group.In the model group and the acupuncture group,the model of rheumatoid arthritis was established by intradermal injection of 50μL of incomplete Freund's adjuvant to the left and right hindpaw of both groups.In the acupuncture group,acupuncture was performed on bilateral "Shenshu"and "Zusanli"points,once a day,6 times a week,last 3 weeks,and rest in the middle of treatment for 1 day.The serum levels of IL-4 and TNF-αwere detected by ELISA.Results:Compared with the blank group,the concentration of IL-4 in the serum of the model group mice decreased,and the concentration of TNF-αincreased significantly(P<0.01);Compared with the model group,mice in the acupuncture group had the higher concentration of serum IL-4 and the lower concentration of TNF-α,with significant difference(P<0.05).Conclusion:Acupuncture can reduce the concentration of serum IL-4 in rats with rheumatoid arthritis,increase the content of IL-4,regulate the imbalance of Th1/Th2 cytokines in RA process,showing apositive guiding role in the clinical control of the development of rheumatoid arthritis.
Objective:To observe the effect of acupuncture on serum interleukin-4(IL-4)and tumor necrosis factor-α(TNF-α)in mice with rheumatoid arthritis and to explore the mechanism of the effect of acupuncture on rheumatoid arthritis(RA).Methods:Twenty-four male mice were randomly divided into three groups,including blank group,RA model group and acupuncture group.In the model group and the acupuncture group,the model of rheumatoid arthritis was established by intradermal injection of 50μL of incomplete Freund's adjuvant to the left and right hindpaw of both groups.In the acupuncture group,acupuncture was performed on bilateral "Shenshu"and "Zusanli"points,once a day,6 times a week,last 3 weeks,and rest in the middle of treatment for 1 day.The serum levels of IL-4 and TNF-αwere detected by ELISA.Results:Compared with the blank group,the concentration of IL-4 in the serum of the model group mice decreased,and the concentration of TNF-αincreased significantly(P<0.01);Compared with the model group,mice in the acupuncture group had the higher concentration of serum IL-4 and the lower concentration of TNF-α,with significant difference(P<0.05).Conclusion:Acupuncture can reduce the concentration of serum IL-4 in rats with rheumatoid arthritis,increase the content of IL-4,regulate the imbalance of Th1/Th2 cytokines in RA process,showing apositive guiding role in the clinical control of the development of rheumatoid arthritis.
引文
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[11]任舒婷,李玉生.Th1/Th2失衡对于类风湿性关节炎炎症损伤的临床意义[J].标记免疫分析与临床,2015,22(9):864-866.
[12]SKAPENKO A,LEIPE J,LIPSKY PE,et al.The role of the T cell inautoimmune inflammation[J].Arthritis Res Ther,2005(7):4-14.
[13]吴利,袁斓,兰颖.针灸对类风湿性关节炎免疫功能影响的动物实验研究进展[J].现代临床医学,2015(5):326-328.
[14]黄凯裕,梁爽,许岳亭,等.基于数据挖掘的针灸治疗类风湿性关节炎的主穴运用规律分析[J].时珍国医国药,2015(11):2792-2795.
[15]岳静,张亚楠,季旭明,等.类风湿性关节炎病证结合动物模型的研究进展[J].辽宁中医杂志,2016(5):1095-1097.
[16]陆声,梁崇礼,林月秋.类风湿性关节炎动物模型应用研究进展[J].中国比较医学杂志,2001,11(2):112-114.
[2]任舒婷,李玉生.Th1/Th2失衡对于类风湿性关节炎炎症损伤的临床意义[J].标记免疫分析与临床,2015,22(9):864.
[3]郭明,安高,封桂英,等.CD4+T细胞亚群在类风湿性关节炎中的研究进展[J].细胞与分子免疫学杂志,2014,30(9):1004-1007.
[4]郭丽红,刘长信,田贵花,等.电针对RA模型大鼠VEFG影响的实验研究[J].中医药学报,2015,43(5):16-19.
[5]郭丽红,刘长信,田贵花,等.电针对类风湿性关节炎大鼠血清及膝关节白介素-17和白介素-23的影响[J].针刺研究,2015,40(2):141-145.
[6]张瑞.电针对CIA模型大鼠TNF-α、IL-1和ICAM-1影响的实验研究[D].北京:北京中医药大学,2016.
[7]黄敬群,刘久红,李伟中,等.槲皮素对急性痛风性关节炎大鼠的治疗作用及其对肝功能的影响[J].山东医药,2015(28):27-29.
[8]余曙光,徐斌主编.实验针灸学[M].北京:人民卫生出版社,2012:269-272.
[9]高俊,丁真奇.细胞因子在类风湿性关节炎中作用的研究现状[J].医学综述,2006,12(5):289-291.
[10]窦肇华,张远强,郭顺根.免疫细胞学与疾病[M].北京:中国医药科技出版社,2004:458.
[11]任舒婷,李玉生.Th1/Th2失衡对于类风湿性关节炎炎症损伤的临床意义[J].标记免疫分析与临床,2015,22(9):864-866.
[12]SKAPENKO A,LEIPE J,LIPSKY PE,et al.The role of the T cell inautoimmune inflammation[J].Arthritis Res Ther,2005(7):4-14.
[13]吴利,袁斓,兰颖.针灸对类风湿性关节炎免疫功能影响的动物实验研究进展[J].现代临床医学,2015(5):326-328.
[14]黄凯裕,梁爽,许岳亭,等.基于数据挖掘的针灸治疗类风湿性关节炎的主穴运用规律分析[J].时珍国医国药,2015(11):2792-2795.
[15]岳静,张亚楠,季旭明,等.类风湿性关节炎病证结合动物模型的研究进展[J].辽宁中医杂志,2016(5):1095-1097.
[16]陆声,梁崇礼,林月秋.类风湿性关节炎动物模型应用研究进展[J].中国比较医学杂志,2001,11(2):112-114.