中期因子在涎腺腺样囊性癌组织中的表达及意义
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摘要
目的探讨中期因子(midkine,MK)在涎腺腺样囊性癌组织中的表达及其与发生嗜神经侵袭的关系。方法采用免疫组织化学SP法检测35例涎腺腺样囊性癌组织和20例正常涎腺组织中MK蛋白的表达情况,并分析其与嗜神经侵袭的关系。结果 MK在涎腺腺样囊性癌组织中高表达,阳性表达率为82.9%(29/35),在正常涎腺组织中低表达,阳性表达率为10.0%(2/20),二者间差异有统计学意义(P<0.01);MK的表达与患者的年龄、性别以及病理分型无关(P>0.05);与肿瘤是否发生嗜神经侵袭有关(P<0.05)。结论 MK蛋白可能参与涎腺腺样囊性癌的发生,其表达水平的升高可能与涎腺腺样囊性癌发生嗜神经侵袭有关。
Objective To study the expression of midkine(MK) in salivary adenoid cystic carcinoma(SACC) and its relationship with perineural invasion(PNI). Methods Immunohistochemistry staining(SP method) for MK was performed on 35 SACC and 20 normal salivary gland tissues(NSG). Then,the correlation between the expression of MK and PNI was analyzed. Results In SACC,MK was highly expressed and the positive rate was 82.9%( 29/35) while it was lowly expressed and the positive rate was 10.0%(2/20) in NSG,which showed significant difference(P<0.01). There was no relationship between the expression of MK and age,sex,pathologic types(P>0.05). However,there was significant difference between PNI cases and non-PNI cases(P<0.05). Conclusions MK may be associated with generation of SACC,and its overexpression may be contributed to the perineural invasion in SACC patients.
Objective To study the expression of midkine(MK) in salivary adenoid cystic carcinoma(SACC) and its relationship with perineural invasion(PNI). Methods Immunohistochemistry staining(SP method) for MK was performed on 35 SACC and 20 normal salivary gland tissues(NSG). Then,the correlation between the expression of MK and PNI was analyzed. Results In SACC,MK was highly expressed and the positive rate was 82.9%( 29/35) while it was lowly expressed and the positive rate was 10.0%(2/20) in NSG,which showed significant difference(P<0.01). There was no relationship between the expression of MK and age,sex,pathologic types(P>0.05). However,there was significant difference between PNI cases and non-PNI cases(P<0.05). Conclusions MK may be associated with generation of SACC,and its overexpression may be contributed to the perineural invasion in SACC patients.
引文
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[14]Kadomatsu K,Tomomura M,Muramatsu T.c DNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis[J].Biochem Biophys Res Commun,1988,151(3):1312-1318.
[15]Murasugi A,Tohma-Aiba Y.Production of native recombinant human midkine in the yeast,Pichia pastoris[J].Protein Expr Purif,2003,27(2):244-252.
[16]Uzun S,Kaya B,Celik K,et al.The role of midkine in the inflammatory process and its correlation with other inflammatory markers in renal transplant recipients[J].Int J Artif Organs,2016,39(6):277-281.
[17]Sakamoto K,Kadomatsu K.Midkine in the pathology of cancer,neural disease,and inflammation[J].Pathol Int,2012,62(7):445-455.
[18]Yao J,Li WY,Gao SG.The advances of Midkine with peripheral invasion in pancreatic cancer[J].Am J Cancer Res,2015,5(9):2912-2917.
[19]陈俊,陈林林.Midkine在口腔颌面部肿瘤中的研究[J].实用医学杂志,2012,28(2):327-328.
[20]Ju J,Li Y,Chai J,et al.The role of perineural invasion on head and neck adenoid cystic carcinoma prognosis:a systematic review and meta-analysis[J].Oral Surg Oral Med Oral Pathol Oral Radiol,2016,122(6):691-701.
[2]Li L,Li Y,Wen Y,et al.Clinical analysis of salivary gland tumor cases in West China in past 50 years[J].Oral Oncol,2008,44(2):187-192.
[3]Gondivkar SM,Gadbail AR,Chole R,et al.Perineural invasion in cancer[J].Cancer,2009,115(15):3379-3391.
[4]Gondivkar SM,Gadbail AR,Chole R,et al.Adenoid cystic carcinoma:A rare clinical entity and literature review[J].Oral Oncol,2011,47(4):231-236.
[5]Amit M,Binenbaum Y,Trejo-Leider L,et al.International collaborative validation of intraneural invasion as a prognostic marker in adenoid cystic carcinoma of the head and neck[J].Head Neck,2015,37(7):1038-1045.
[6]Yao J,Li W,Li S,et al.Midkine promotes perineural invasion in human pancreatic cancer[J].World J Gastroenterol,2014,20(11):3018-3024.
[7]陆巍,童汉兴,周恒花,等.乳腺癌组织中中期因子的表达及其临床意义[J].中国临床医学,2008,15(3):433-434.
[8]Yamashita T,Shimada H,Tanaka S,et al.Serum midkine as a biomarker for malignancy,prognosis,and chemosensitivity in head and neck squamous cell carcinoma[J].Cancer Medicine,2016,5(3):415-425.
[9]Huang H,Lu Y,Min L,et al.Phospholipid scramblase 1 interacts with Midkine and regulates hepatic cancer cell proliferation and migration[J].Clin Lab,2015,61(10):1501-1508.
[10]Li F,Tian P,Zhang J,et al.The clinical and prognostic significance of midkine in breast cancer patients[J].Tumour Biol,2015,38(12):9789-9794.
[11]Masui M,Okui T,Shimo T,et al.Novel midkine inhibitor i MDK inhibits tumor growth and angiogenesis in oral squamous cell carcinoma[J].Anticancer Res,2016,36(6):2775-2781.
[12]Ota T,Ota K,Jono H,et al.Midkine expression in malignant salivary gland tumors and its role in tumor angiogenesis[J].Oral Oncol,2010,49(9):657-661.
[13]陈俊,李姬梅,李伟,等.中期因子和微血管密度在涎腺腺样囊性癌组织中的表达[J].华西口腔医学杂志,2016,34(2):189-193.
[14]Kadomatsu K,Tomomura M,Muramatsu T.c DNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesis[J].Biochem Biophys Res Commun,1988,151(3):1312-1318.
[15]Murasugi A,Tohma-Aiba Y.Production of native recombinant human midkine in the yeast,Pichia pastoris[J].Protein Expr Purif,2003,27(2):244-252.
[16]Uzun S,Kaya B,Celik K,et al.The role of midkine in the inflammatory process and its correlation with other inflammatory markers in renal transplant recipients[J].Int J Artif Organs,2016,39(6):277-281.
[17]Sakamoto K,Kadomatsu K.Midkine in the pathology of cancer,neural disease,and inflammation[J].Pathol Int,2012,62(7):445-455.
[18]Yao J,Li WY,Gao SG.The advances of Midkine with peripheral invasion in pancreatic cancer[J].Am J Cancer Res,2015,5(9):2912-2917.
[19]陈俊,陈林林.Midkine在口腔颌面部肿瘤中的研究[J].实用医学杂志,2012,28(2):327-328.
[20]Ju J,Li Y,Chai J,et al.The role of perineural invasion on head and neck adenoid cystic carcinoma prognosis:a systematic review and meta-analysis[J].Oral Surg Oral Med Oral Pathol Oral Radiol,2016,122(6):691-701.