中期因子和微血管密度在涎腺腺样囊性癌组织中的表达
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摘要
目的探讨中期因子(MK)、微血管密度(MVD)在涎腺腺样囊性癌(SACC)组织中的表达和意义,以及两者之间表达的相关性。方法采用免疫组织化学方法(SP法)检测60例SACC组织及26例正常涎腺组织中MK、MVD的表达状况及两者之间的相关性。结果 MK在SACC组织中高表达,阳性表达率为70.0%(42/60),正常组织中未见MK表达,二者间差异有统计学意义(P<0.05)。SACC中MVD计数为38.73±8.96,正常涎腺组织中MVD计数为11.15±3.33,二者间差异有统计学意义(P<0.05)。MK、MVD的表达与年龄、性别、分型无关(P>0.05);与肿瘤的大小、淋巴结转移、TNM分期有关(P<0.05)。MK和MVD在SACC中的表达具有正相关性(r=0.560,P<0.05)。结论 MK蛋白的表达、MVD计数的增加与SACC的发生有关,可能是SACC早期诊断指标之一。
Objective This study aimed to investigate the expression of midkine(MK) and microvessel density(MVD) in patients with salivary adenoid cystic carcinoma(SACC) and its clinical significance, as well as detect the correlation between the expression of MK and MVD in SACC. Methods Immunohistochemistry analysis(SP method) for MK and MVD were performed on 60 cases of SACC and 26 cases of normal salivary gland tissue. The expression of MK and MVD, as well as the correlation between the expression of MK and MVD in SACC were detected. Results In SACC, the MK expression rate was 70.0%(42/60), and MK was not expressed in normal tissue. Statistical significance was found between SACC and normal tissue(P<0.05). The MVD values in SACC and normal salivary gland tissues were 38.73±8.96 and 11.15±3.33, respectively. These values were statistically significant(P<0.05). The expression levels of MK and MVD were unrelated to age, gender, and type in SACC(P>0.05), but correlated with tumor size, lymph node metastasis, and tumor–node–metastasis in SACC(P<0.05). The expression of MK and MVD was positively correlated with SACC(r=0.560, P<0.05). Conclusion SACC is correlated with the expression of MK protein and the increase in MVD, which may be some of the early diagnostic markers in SACC.
Objective This study aimed to investigate the expression of midkine(MK) and microvessel density(MVD) in patients with salivary adenoid cystic carcinoma(SACC) and its clinical significance, as well as detect the correlation between the expression of MK and MVD in SACC. Methods Immunohistochemistry analysis(SP method) for MK and MVD were performed on 60 cases of SACC and 26 cases of normal salivary gland tissue. The expression of MK and MVD, as well as the correlation between the expression of MK and MVD in SACC were detected. Results In SACC, the MK expression rate was 70.0%(42/60), and MK was not expressed in normal tissue. Statistical significance was found between SACC and normal tissue(P<0.05). The MVD values in SACC and normal salivary gland tissues were 38.73±8.96 and 11.15±3.33, respectively. These values were statistically significant(P<0.05). The expression levels of MK and MVD were unrelated to age, gender, and type in SACC(P>0.05), but correlated with tumor size, lymph node metastasis, and tumor–node–metastasis in SACC(P<0.05). The expression of MK and MVD was positively correlated with SACC(r=0.560, P<0.05). Conclusion SACC is correlated with the expression of MK protein and the increase in MVD, which may be some of the early diagnostic markers in SACC.
引文
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[3]Weidner N,Folkman J,Pozza F,et al.Tumor angiogenesis:a new significant and independent prognostic indicator in early-stage breast carcinoma[J].J Natl Cancer Inst,1992,84(24):1875-1887.
[4]Iwashita N,Muramatsu H,Toriyama K,et al.Expression of midkine in normal and burn sites of rat skin[J].Burns,1999,25(2):119-124.
[5]Kadomatsu K,Muramatsu T.Midkine and pleiotrophin in neural development and cancer[J].Cancer Lett,2004,204(2):127-143.
[6]陆巍,童汉兴,周恒花,等.乳腺癌组织中中期因子的表达及其临床意义[J].中国临床医学,2008,15(3):433-434.Lu W,Tong HX,Zhou HH,et al.Clinical significance of midkine expression in breast cancer[J].Clin Med J Chin,2008,15(3):433-434.
[7]栾兆吉,诸兰艳.中期因子在肺癌中的研究进展[J].国际呼吸杂志,2010,30(4):229-233.Luan ZJ,Zhu LY.Research progress of midkine in lung cancer[J].Int J Respir,2010,30(4):229-233.
[8]石清涛,宁晓明,冯占军.中期因子在胃癌中的表达及其意义[J].肿瘤防治研究,2008,35(1):30-32.Shi QT,Ning XM,Feng ZJ.Expression of midkine in gas-tric cancer and its clinical significance[J].Cancer Res Prevent Treat,2008,35(1):30-32.
[9]王耀先,于辉,陈秀玮,等.宫颈癌组织中中期因子表达与临床病理特征的关系[J].哈尔滨医科大学学报,2008,42(6):622-624.Wang YX,Yu H,Chen XW,et al.Relationship between the expression of midkine factor and the character of clinical pathology in cervical cancer[J].J Harbin Med Uni,2008,42(6):622-624.
[10]高顺姬,李广伦.中期因子及血管内皮生长因子在多发性骨髓瘤患者骨髓中的表达及意义[J].中国实验血液学杂志,2009,17(6):1464-1467.Gao SJ,Li GL.Expression of midkine and vascular endothelial growth factor in bone marrow of patients with multiple myeloma and its significance[J].J Exp Hematol,2009,17(6):1464-1467.
[11]李光宇,毛雄辉,孙冀.Cyclin D1和中期因子在口腔鳞状细胞癌中表达的实验研究[J].口腔颌面外科杂志,2008,18(6):390-393.Li GY,Mao XH,Sun J.Expression of Cyclin D1 and midkine in oral squamous cell carcinoma[J].J Oral Maxillofac Surg,2008,18(6):390-393.
[12]Ikematsu S,Okamoto K,Yoshida Y,et al.High levels of urinary midkine in various cancer patients[J].Biochem Biophys Res Commun,2003,306(2):329-332.
[13]Brewer CA,Setterdahl JJ,Li MJ,et al.Endoglin expression as a measure of microvessel density in cervical cancer[J].Obstet Gynecol,2000,96(2):224-228.
[14]李丙华,徐娅苹,陈鸣,等.CD105与CD34在喉癌微血管中的表达及临床意义[J].浙江预防医学,2004,16(6):18-19.Li BH,Xu YP,Chen M,et al.A study on the expression of CD105 and CD34 in microvessel and its clinical significance in laryngocarcinoma patients[J].Zhejiang J Prevent Med,2004,16(6):18-19.
[15]Mashour GA,Ratner N,Khan GA,et al.The angiogenic factor midkine is aberrantly expressed in NF1-deficient Schwann cells and is a mitogen for neurofibroma-derived cells[J].Oncogene,2001,20(1):97-105.