近红外无损检测安胎丸中关键质控指标成分的含量
|
摘要
目的:开发一种简单快速的近红外光谱无损检测方法,对安胎丸中六种质控指标成分的含量进行检测。方法:以安胎丸中的阿魏酸、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、洋川芎内酯A的HPLC含量为实际值,匹配所测定的NIR光谱图,分别进行光谱预处理、最佳波段选择,结合偏最小二乘法分别建立六种质控指标成分的近红外定量分析模型,并对外部样本进行含量预测。结果:根据交叉验证的标准误差(SECV)选择最佳模型维数(LV),各校正模型的R2分别为0.9699、0.9518、0.9625、0.9450、0.9474、0.8599。外部验证结果中,相关系数R2均在0.90以上。结论:建立的定量模型预测效果良好,基于近红外光谱无损检测法是一种快速简便的测定安胎丸质控指标成分的方法。
Objective: A simple and fast near infrared spectroscopy was developed to have a nondestructive test of the content of 6 quality control index components in Antai pills. Methods: The HPLC values for the active ingredients content of ferulic acid, baicalin, wogonoside, baicalein, wogonin, senkyunolide A in Antai pills were set as actual values, that match the measured NIR spectral figures, Each of the spectral was pre-processed and best band selected, to establish the quantitative analysis model of six quality control index components by the partial least squares. The content of external samples was predicted. Results: In accordance with the standard error of cross-validation(SECV)the best model dimension(LV) was selected. The correlation coefficient R2 values of the 6 models of ferulic acid, baicalin, wogonoside,baicalein, wogonin, senkyunolide A were 0.9699, 0.9518, 0.9625, 0.9450, 0.9474, and 0.8599. In the external validation results, the correlation coefficient R2 was above 0.90. Conclusion: The establishment of quantitative models predict effects are good,, and the detection method based on near-infrared spectroscopy is a fast and simple measurements of Antai pills of quality control method.
Objective: A simple and fast near infrared spectroscopy was developed to have a nondestructive test of the content of 6 quality control index components in Antai pills. Methods: The HPLC values for the active ingredients content of ferulic acid, baicalin, wogonoside, baicalein, wogonin, senkyunolide A in Antai pills were set as actual values, that match the measured NIR spectral figures, Each of the spectral was pre-processed and best band selected, to establish the quantitative analysis model of six quality control index components by the partial least squares. The content of external samples was predicted. Results: In accordance with the standard error of cross-validation(SECV)the best model dimension(LV) was selected. The correlation coefficient R2 values of the 6 models of ferulic acid, baicalin, wogonoside,baicalein, wogonin, senkyunolide A were 0.9699, 0.9518, 0.9625, 0.9450, 0.9474, and 0.8599. In the external validation results, the correlation coefficient R2 was above 0.90. Conclusion: The establishment of quantitative models predict effects are good,, and the detection method based on near-infrared spectroscopy is a fast and simple measurements of Antai pills of quality control method.
引文
1陈粲文,宋粉云,李华,等.安胎丸UPLC指纹图谱及模式识别研究.药物分析杂志,2016,36(3):465-472.
2 卫生部药品标准.中药成方制剂.第一册.1989:76.
3 孙旌文,马洪涛,许世辉.HPLC法测定安胎丸中阿魏酸的含量.安徽医药,2010,14(3):296.
4 欧妮,陈少萍,王丽丽,等.HPLC法测定安胎丸中白术内酯I的含量.轻工科技,2014(12)∶110.
5 黎春彤,刘皈阳,马建丽,等.HPLC法测定安胎丸中黄芩苷、黄芩素、汉黄芩素的含量.解放军药学学报,2015,31(3):254.
6 胡寿荣.高效液相色谱法测定安胎丸中黄芩苷的含量.中国实验方剂学志,2010,16(4):66.
7 胡昌勤.近红外光谱法快速分析药品.北京:化学工业出版社出版,2014,29(10):1105-1108.
8 张迪文,白雁,谢彩侠,等.近红外光谱法快速测定女贞子药材中女贞昔的含量.中国药房,2016,36(27):5139-5133.
9 朱斌.近红外漫反射光谱法的应用研究.第二军医大学,2003,04.
10 曲佳,王秋珍,王杰.近红外光谱法快速鉴别不同厂家的天王补心丸(小蜜丸).药物评价研究,2014,37(4):346-348.
11 王雪利,马晋芳,彭银,等.安胎丸HPLC指纹图谱研究.中药材,2018,41(1):160-164.
12 Pan W J,Ma J F,Xiao X,et al.Near-infrared spectroscopy assay of key quality-indicative ingredients of tongkang tablets.Aerican Association of Pharmaccutical Scientists,2016,18(3)∶913-919.
13 周昭露,李杰,黄生权,等.近红外光谱技术在中药质量控制应用中的化学计量学建模:综述和展望.化工进展,2016,35(6):1627-1645.
14 刘黎锋,梁洁,马晋芳,等.近红外光谱法快速测定黄芪提取过程中毛蕊异黄酮葡萄糖苷含量.江西中医药,2016,397(47):68-70.
15 孙灵霞,陈锦屏,赵改名,等.化学计量学在食品分析中的应用研究进展.食品工业科技,2012,07:444-452.
16 吴习宇,祝诗平,黄华,等.近红外光谱技术鉴别花椒产地.光谱学与光谱分析,2018,38(1):68-72.
17 田旷达,邱凯贤,李祖红,等.近红外光谱法结合最小二乘支持向量机测定烟叶中钙、镁元素.光谱学与光谱分析,2014,34(12):3263-3266.
18 许崇瑶,叶正良,李德坤,等.近红外光谱法快速测定枳壳提取液中柚皮苷你和新橙皮苷含量.中国实验方剂学杂志,2014,11(6):86-90.
19 陆婉珍.现代近红外光谱分析技术.中国石化出版社,2010.
2 卫生部药品标准.中药成方制剂.第一册.1989:76.
3 孙旌文,马洪涛,许世辉.HPLC法测定安胎丸中阿魏酸的含量.安徽医药,2010,14(3):296.
4 欧妮,陈少萍,王丽丽,等.HPLC法测定安胎丸中白术内酯I的含量.轻工科技,2014(12)∶110.
5 黎春彤,刘皈阳,马建丽,等.HPLC法测定安胎丸中黄芩苷、黄芩素、汉黄芩素的含量.解放军药学学报,2015,31(3):254.
6 胡寿荣.高效液相色谱法测定安胎丸中黄芩苷的含量.中国实验方剂学志,2010,16(4):66.
7 胡昌勤.近红外光谱法快速分析药品.北京:化学工业出版社出版,2014,29(10):1105-1108.
8 张迪文,白雁,谢彩侠,等.近红外光谱法快速测定女贞子药材中女贞昔的含量.中国药房,2016,36(27):5139-5133.
9 朱斌.近红外漫反射光谱法的应用研究.第二军医大学,2003,04.
10 曲佳,王秋珍,王杰.近红外光谱法快速鉴别不同厂家的天王补心丸(小蜜丸).药物评价研究,2014,37(4):346-348.
11 王雪利,马晋芳,彭银,等.安胎丸HPLC指纹图谱研究.中药材,2018,41(1):160-164.
12 Pan W J,Ma J F,Xiao X,et al.Near-infrared spectroscopy assay of key quality-indicative ingredients of tongkang tablets.Aerican Association of Pharmaccutical Scientists,2016,18(3)∶913-919.
13 周昭露,李杰,黄生权,等.近红外光谱技术在中药质量控制应用中的化学计量学建模:综述和展望.化工进展,2016,35(6):1627-1645.
14 刘黎锋,梁洁,马晋芳,等.近红外光谱法快速测定黄芪提取过程中毛蕊异黄酮葡萄糖苷含量.江西中医药,2016,397(47):68-70.
15 孙灵霞,陈锦屏,赵改名,等.化学计量学在食品分析中的应用研究进展.食品工业科技,2012,07:444-452.
16 吴习宇,祝诗平,黄华,等.近红外光谱技术鉴别花椒产地.光谱学与光谱分析,2018,38(1):68-72.
17 田旷达,邱凯贤,李祖红,等.近红外光谱法结合最小二乘支持向量机测定烟叶中钙、镁元素.光谱学与光谱分析,2014,34(12):3263-3266.
18 许崇瑶,叶正良,李德坤,等.近红外光谱法快速测定枳壳提取液中柚皮苷你和新橙皮苷含量.中国实验方剂学杂志,2014,11(6):86-90.
19 陆婉珍.现代近红外光谱分析技术.中国石化出版社,2010.