新型化合物对六种植物种子萌发及生理指标的影响
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摘要
为验证新型化学合成物乙二酸二乙胺基乙醇酯柠檬酸盐(胺基乙醇酯)对不同种类植物所产生的生理学效应,采用浸种萌发法探究胺基乙醇酯对六种植物(高丹草,紫花苜蓿,紫云英,萝卜,毛豆,小麦)萌发过程中三项萌发相关指标(发芽率,发芽势,发芽指数)及幼苗期生长过程中的六项生理指标(总糖含量,根系活力,硝酸还原酶活性,超氧化物歧化酶活性,过氧化氢酶活性,氧自由基产生速率)所产生的影响。与市场上已大量存有及使用的具有一定同源性的生长调节剂己酸二乙氨基乙醇酯(DA-6)相比较,对相关植物产生的生理效应进行显著性比对分析。结果显示胺基乙醇酯在抑制氧自由基产生速率方面效果不是十分显著,对其余指标的效果方面胺基乙醇酯均优于现有的DA-6。
To verify the plant physiology of the novel chemical composition diethylaminoethanol adipate citrate(Aminoethanol ester) on different plants, germination soaking method was used to explore the effect of aminoethanol ester on the three germination indicators(germination rate, germination potential, germination index) of six kinds of plants(Sorghum Hybrid Sudangrass, Medicago sativa L., Astragalus sinicus L., Raphanus sativus L.,Glycine max(Linn.) Merr., Triticum aestivum L.) during the germination period, and the six physiological indicators during the seedling stage(total sugar content, root activity, nitrate reductase activity, superoxide dismutase activity,catalase activity, oxygen radical production rate). Comparing with the existing and widely-used homologous growth regulator diethylaminoethanol ester(DA-6) in the market, the physiological effects of related plants were compared significantly. The results showed that the effect of aminoethanol esters in inhibiting the production rate of oxygen free radicals was not very significant, and the effect of aminoethanol esters on the remaining indicators was better than that of the existing DA-6.
To verify the plant physiology of the novel chemical composition diethylaminoethanol adipate citrate(Aminoethanol ester) on different plants, germination soaking method was used to explore the effect of aminoethanol ester on the three germination indicators(germination rate, germination potential, germination index) of six kinds of plants(Sorghum Hybrid Sudangrass, Medicago sativa L., Astragalus sinicus L., Raphanus sativus L.,Glycine max(Linn.) Merr., Triticum aestivum L.) during the germination period, and the six physiological indicators during the seedling stage(total sugar content, root activity, nitrate reductase activity, superoxide dismutase activity,catalase activity, oxygen radical production rate). Comparing with the existing and widely-used homologous growth regulator diethylaminoethanol ester(DA-6) in the market, the physiological effects of related plants were compared significantly. The results showed that the effect of aminoethanol esters in inhibiting the production rate of oxygen free radicals was not very significant, and the effect of aminoethanol esters on the remaining indicators was better than that of the existing DA-6.
引文
Aravind P.,and Prasad M.N.V.,2005,Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate-glutathione cycle and glutathione metabolism,Plant Physiol.Biochem.,43(2):107-116
Di N.,2016,Effect of irrigation depth control on root morphology and root vigor of winter wheat,Thisis for M.S.,Taiyuan University of Technology,Supervisors:Ma J.J.,and Bai J.Z.,pp.1-53(狄楠,2016,灌水深度对冬小麦根系形态分布及根系活力的影响,硕士学位论文,太原理工大学,导师:马娟娟,白继中,pp.1-53)
Feng Y.N.,2010,Regulation effects of plant growth regulators on seedlings building,yield and quality of soybean,Thisis for M.S.,Heilongjiang August First Land Reclamation University,Supervisor:Feng N.J.,pp.1-76(冯亚楠,2010,植物生长调节剂对大豆苗建成及产量品质的调控效应,硕士学位论文,黑龙江八一农垦大学,导师:冯乃杰,pp.1-76)
Han D.L.,Han L.B.,and Zhou X.J.,2008,Research of salt tolerance in alfalfa,Zhongzi(Seed),27(6):1-4(韩德梁,韩烈保,周晓静,2008,中苜一号紫花苜蓿耐盐性研究,种子,27(6):1-4)
Lin Y.S.,Chen M.C.,Shen M.S.,and Hong W.L.,1998,Studies on effect of content and component of steviol glucosides with DA-6 in Stevia rebaudiana Bertoni,Zhongguo Tangliao(Sugar Crops of China),(3):12-15(林永顺,陈睦传,沈明山,洪维廉,1998,DA-6对甜菊叶片糖苷含量和组分影响研究,中国糖料,(3):12-15)
Liu J.T.,Zhu H.S.,Wen Q.F.,Wang B.,Zhang Q.R.,Chen M.D.,Lin H.,and Xue Z.Z.,2017,Isolation and expression catalase CAT2 gene from Luffa cylindrical,Zhongguo Xibao Shengwuxue Xuebao(Chinese Journal of Cell Biology),39(8):1076-1083(刘建汀,朱海生,温庆放,王彬,张前荣,陈敏氡,林珲,薛珠政,2017,丝瓜过氧化氢酶CAT2基因的分离及表达分析,中国细胞生物学学报,39(8):1076-1083)
Liu W.,Qu L.H.,Liu H.Q.,Yang D.C.,and Liu X.,2008,Effects of chilling stress on protective enzymes activities and oxygen free radical of grapes,Beifang Yuanyi(Northern Horticulture),(5):21-24(刘伟,曲凌慧,刘洪庆,杨德翠,刘新,2008,低温胁迫对葡萄保护酶和氧自由基的影响,北方园艺,(5):21-24)
Wei H.Z.,Zhou J.L.,Pan L.Q.,Zhang C.L.,Li Y.L.,and Ren H.,2017,Effects of copper sulfate solution concentration and soaking time on seed germination and growth,Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),15(10):4184-4189(韦海忠,周嘉玲,潘丽芹,张成磊,李源龙,任欢,2017,硫酸铜溶液浓度和浸泡时间对罗汉松种子发芽及生长量的影响,分子植物育种,15(10):4184-4189)
Wu W.P.,Yuan Y.L.,Ding F.B.,Xun L.L.,Wang Q.,Bu J.,and Li S.J.,2018,Study on seed germination of three kinds of peren nial flower,Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),16(2):578-582(吴永朋,原雅玲,丁芳兵,寻路路,王琪,卜洁,李淑娟,2018,三种宿根花卉种子萌发研究,分子植物育种,16(2):578-582)
Xu Y.Y.,Jin J.,2017,Effects of rare earth micro fertilizers on rice seedling I under acid rain stress,Jiyingzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(9):3893-3900(徐义英,金琎,2017,稀土微肥对酸雨胁迫下水稻幼苗期Ⅰ的影响,基因组学与应用生物学,36(9):3893-3900)
Zhang H.J.,Chen D.D.,and Song Y.X.,2017,Effects of Met-Se with different concentrations on seed germination and seedling growth of pumpkin,Jiyinzuxue Yu Yingyong Sheng wuxue(Genomics and Applied Biology),36(3):1072-1076(张慧君,陈丹丹,宋运贤,2017,不同浓度蛋氨酸硒对南瓜种子的萌发和幼苗生长的影响,基因组学与应用生物学,36(3):1072-1076)
Di N.,2016,Effect of irrigation depth control on root morphology and root vigor of winter wheat,Thisis for M.S.,Taiyuan University of Technology,Supervisors:Ma J.J.,and Bai J.Z.,pp.1-53(狄楠,2016,灌水深度对冬小麦根系形态分布及根系活力的影响,硕士学位论文,太原理工大学,导师:马娟娟,白继中,pp.1-53)
Feng Y.N.,2010,Regulation effects of plant growth regulators on seedlings building,yield and quality of soybean,Thisis for M.S.,Heilongjiang August First Land Reclamation University,Supervisor:Feng N.J.,pp.1-76(冯亚楠,2010,植物生长调节剂对大豆苗建成及产量品质的调控效应,硕士学位论文,黑龙江八一农垦大学,导师:冯乃杰,pp.1-76)
Han D.L.,Han L.B.,and Zhou X.J.,2008,Research of salt tolerance in alfalfa,Zhongzi(Seed),27(6):1-4(韩德梁,韩烈保,周晓静,2008,中苜一号紫花苜蓿耐盐性研究,种子,27(6):1-4)
Lin Y.S.,Chen M.C.,Shen M.S.,and Hong W.L.,1998,Studies on effect of content and component of steviol glucosides with DA-6 in Stevia rebaudiana Bertoni,Zhongguo Tangliao(Sugar Crops of China),(3):12-15(林永顺,陈睦传,沈明山,洪维廉,1998,DA-6对甜菊叶片糖苷含量和组分影响研究,中国糖料,(3):12-15)
Liu J.T.,Zhu H.S.,Wen Q.F.,Wang B.,Zhang Q.R.,Chen M.D.,Lin H.,and Xue Z.Z.,2017,Isolation and expression catalase CAT2 gene from Luffa cylindrical,Zhongguo Xibao Shengwuxue Xuebao(Chinese Journal of Cell Biology),39(8):1076-1083(刘建汀,朱海生,温庆放,王彬,张前荣,陈敏氡,林珲,薛珠政,2017,丝瓜过氧化氢酶CAT2基因的分离及表达分析,中国细胞生物学学报,39(8):1076-1083)
Liu W.,Qu L.H.,Liu H.Q.,Yang D.C.,and Liu X.,2008,Effects of chilling stress on protective enzymes activities and oxygen free radical of grapes,Beifang Yuanyi(Northern Horticulture),(5):21-24(刘伟,曲凌慧,刘洪庆,杨德翠,刘新,2008,低温胁迫对葡萄保护酶和氧自由基的影响,北方园艺,(5):21-24)
Wei H.Z.,Zhou J.L.,Pan L.Q.,Zhang C.L.,Li Y.L.,and Ren H.,2017,Effects of copper sulfate solution concentration and soaking time on seed germination and growth,Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),15(10):4184-4189(韦海忠,周嘉玲,潘丽芹,张成磊,李源龙,任欢,2017,硫酸铜溶液浓度和浸泡时间对罗汉松种子发芽及生长量的影响,分子植物育种,15(10):4184-4189)
Wu W.P.,Yuan Y.L.,Ding F.B.,Xun L.L.,Wang Q.,Bu J.,and Li S.J.,2018,Study on seed germination of three kinds of peren nial flower,Fengzi Zhiwu Yuzhong(Molecular Plant Breeding),16(2):578-582(吴永朋,原雅玲,丁芳兵,寻路路,王琪,卜洁,李淑娟,2018,三种宿根花卉种子萌发研究,分子植物育种,16(2):578-582)
Xu Y.Y.,Jin J.,2017,Effects of rare earth micro fertilizers on rice seedling I under acid rain stress,Jiyingzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(9):3893-3900(徐义英,金琎,2017,稀土微肥对酸雨胁迫下水稻幼苗期Ⅰ的影响,基因组学与应用生物学,36(9):3893-3900)
Zhang H.J.,Chen D.D.,and Song Y.X.,2017,Effects of Met-Se with different concentrations on seed germination and seedling growth of pumpkin,Jiyinzuxue Yu Yingyong Sheng wuxue(Genomics and Applied Biology),36(3):1072-1076(张慧君,陈丹丹,宋运贤,2017,不同浓度蛋氨酸硒对南瓜种子的萌发和幼苗生长的影响,基因组学与应用生物学,36(3):1072-1076)