制备具有明显OVA吸附作用的Al(OH)_3佐剂及其在变应性鼻炎大鼠模型建立中的应用
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摘要
目的:制备可明显吸附卵清蛋白(OVA)的Al(OH)_3佐剂并用其建立具备变应性鼻炎(AR)临床客观特征的大鼠模型.方法:通过控制反应条件制备可明显吸附OVA的Al(OH)_3佐剂,对模型组用OVA辅以自制Al(OH)_3佐剂进行基础致敏,继以质量分数为2%OVA生理盐水行鼻内激发.对照组以生理盐水替代自制Al(OH)_3佐剂.采用PCA、行为学评分、Luna染色、HE染色评价模型.结果:1)吸附研究表明自制Al(OH)_3佐剂对OVA具明显吸附作用;2)模型组:PCA(+)证明变应原特异性Ig E产生;行为学评分>5分;Luna染色与HE染色示鼻黏膜嗜酸性粒细胞增多、浸润.对照组无上述变化.结论:所制Al(OH)_3佐剂对OVA具明显吸附作用并在此基础上成功建立了具备Ig E阳性和鼻黏膜嗜酸性粒细胞增多AR临床客观特征的大鼠模型.
Aim: Prepared a new kind of Al(OH)_3adjuvant which has obviously absorbability on ovalbumin,and applied in establishing a rat model with objective clinical characteristics of allergic rhinitis(AR). Methods: Firstly, a new kind of Al(OH)_3adjuvant with obvious adsorption on ovalbumin(OVA) had been prepared by controlling the reaction conditions,then,was used in building AR rat model. The model group was treated by ovalbumin and Al(OH)_3to complete basic sensitization;followed by nasal challenge with 2% ovalbumin saline. The control group used normal saline instead of Al(OH)_3adjuvant. By the passive cutaneous anaphylaxis, the behavioral score, Luna staining,Hematoxylin-eosin staining,the model was comprehensively evaluated. Results: 1) the results of OVA absorption experiments showed our Al(OH)_3could absorb OVA obviously. 2) the model group: passivecutaneous anaphylaxis(+) proved the production of allergen specific Ig E; behavioral score was greater than 5; Luna staining and Hematoxylin-eosin staining showed the nasal mucosa eosinophilia infiltration.The control group had no such changes. Conclusion: Based on the preparation of Al(OH)_3adjuvant with obvious adsorption on ovalbumin,we had successfully established the rat model with allergen specific Ig E(+) and nasal mucosa eosinophilia infiltration two objective clinical characteristics of allergic rhinitis.
Aim: Prepared a new kind of Al(OH)_3adjuvant which has obviously absorbability on ovalbumin,and applied in establishing a rat model with objective clinical characteristics of allergic rhinitis(AR). Methods: Firstly, a new kind of Al(OH)_3adjuvant with obvious adsorption on ovalbumin(OVA) had been prepared by controlling the reaction conditions,then,was used in building AR rat model. The model group was treated by ovalbumin and Al(OH)_3to complete basic sensitization;followed by nasal challenge with 2% ovalbumin saline. The control group used normal saline instead of Al(OH)_3adjuvant. By the passive cutaneous anaphylaxis, the behavioral score, Luna staining,Hematoxylin-eosin staining,the model was comprehensively evaluated. Results: 1) the results of OVA absorption experiments showed our Al(OH)_3could absorb OVA obviously. 2) the model group: passivecutaneous anaphylaxis(+) proved the production of allergen specific Ig E; behavioral score was greater than 5; Luna staining and Hematoxylin-eosin staining showed the nasal mucosa eosinophilia infiltration.The control group had no such changes. Conclusion: Based on the preparation of Al(OH)_3adjuvant with obvious adsorption on ovalbumin,we had successfully established the rat model with allergen specific Ig E(+) and nasal mucosa eosinophilia infiltration two objective clinical characteristics of allergic rhinitis.
引文
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[26]周柳青,刘勇强,张小兵.建立符合人类诊断标准的变应性鼻炎大鼠模型初探[J].甘肃科技,2016,36(22):127-128.ZHOU L Q,LIU Y Q,ZHANG X B.Establishment of a rat model of allergic rhinitis in accordance with the diagnostic criteria[J].Gansu Science and Technology,2016,36(22):127-128.
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[2]BOUSQUET J,SCHUNEMANN H J,SAMOLINSKI B,et al.Allergic rhinitis and its impact on asthma(ARIA):Achievements in 10 years and future needs[J].Journal of Allergy and Clinical Immunology,2012,130(5):1049-1062.
[3]FUJIEDA S,KURONO Y,OKUBO K,et al.Examination,diagnosis and classification for Japanese allergic rhinitis:Japanese guideline[J].Auris Nasus Larynx,2012,39(6):553-556.
[4]BAARS E W,SAVELKOUL H F J.Citrus/Cydonia Comp.Can Restore the Immunological Balance in Seasonal Allergic Rhinitis-Related Immunological Parameters In Vitro[J].Mediators of Inflammation,2008,2008(1):496467.
[5]BOUSQUET J,KHALTAEV N,CRUZ A A,et al.Allergic rhinitis and its impact on asthma(ARIA)2008update(in collaboration with the World Health Organization,GA(2)LEN and Aller Gen)[J].Allergy,2008,63(Suppl 86):8-160.
[6]BARTH H,BERG P A,KLEIN R.Methods for the in vitro determination of an individual disposition towards TH1-or TH2-reactivity by the application of appropriate stimulatory antigens[J].Clinical and Experimental Immunology,2003,134(1):78-85.
[7]胡艳菲,唐方.过敏性鼻炎动物模型研究进展[J].医学研究生学报,2012,25(5):540-543.HU Y F,TANG F.Establishment of animal models of allergic rhinitis:An update[J].Journal of Medical Postgraduates,2012,25(5):540-543.
[8]SEHMI R,WOOD L J,WATSON R,et al.Allergeninduced increases in IL-5 receptor alpha-subunit expression on bone marrow-derived CD34+cells from asthmatic subjects.A novel marker of progenitor cell commitment towards eosinophilic differentiation[J].Journal of Clinical Investigation,1997,100(10):2466-2475.
[9]何金年,谭建成,姚东方,等.鼻内类固醇激素对变应性鼻炎动物模型鼻腔黏膜的影响[J].临床耳鼻咽喉头颈外科杂志,2012,26(2):74-77.HE J N,TAN J C,YAO D F,et al.The effect of intranasal steroid on nasal mucosa in rat model of allergic rhinitis[J].Journal of Clinical Otorhinolaryngology Head and Neck Surgery,2012,26(2):74-77.
[10]赵秀杰,董震,杨占泉.鼻超敏反应实验模型的建立[J].中华耳鼻咽喉科杂志,1993,28(1):17-18.ZHAO X J,DONG Z,YANG Z Q.Establishment of experimental model of nasal hypersensitivity[J].Chinese Journal of Otorhinolaryngology Head and Neck Surgery,1993,28(1):17-18.
[11]陈晴,刘洋,张勤修,等.变应性鼻炎大鼠模型的文献研究[J].世界科学技术-中医药现代化,2015,17(2):372-376.CHEN QING,LIU YANG,ZHANG Q X,et al.Literature Research on Allergic Rhinitis Rat Model[J].Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2015,17(2):372-376.
[12]锡琳,张罗,张伟.变应性鼻炎动物模型若干问题[J].国际耳鼻咽喉头颈外科杂志,2008,32(2):81-84.XI LIN,ZHANG L,ZHANG W.Some problems of animal models of allergic rhinitis[J].International Journal of Otolaryngology-Head and Neck Surgery,2008,32(2):81-84.
[13]李萌,魏肖云,戴启刚,等.变应性鼻炎动物模型的研究现状[J].现代中西医结合杂志,2013,22(12):1360-1363.LI M,WEI X Y,DAI Q G,et al.Current situation of animal models of allergic rhinitis[J].Modern Journal of Integrated Traditional Chinese and Western Med Evaluation of the safety of aluminium adjuvant in the preparation of allergic rhinitis animal model icine,2013,22(12):1360-1363.
[14]范尔钟,锡琳,韩德民,等.铝佐剂在建立变应性鼻炎动物模型中的安全性研究[J].中华耳鼻咽喉头颈外科杂志,2009,44(8):664-668.FAN E Z,XI L,HAN D M,et al.Evaluation of the safety of aluminium adjuvant in the preparation of allergic rhinitis animal model[J].Chinese Journal of Otorhinolaryngology Head and Neck Surgery,2009,44(8):664-668.
[15]GUPTA R K.Aluminum compounds as vaccine adjuvants[J].Advanced Drug Delivery reviews,1998,32(3):155-172.
[16]栗克喜.疫苗中的铝佐剂专题讨论会[J].国外医学(预防·诊断·治疗用生物制品分册),2003,26(1):27-30.LI K X.Symposium on aluminum adjuvants in vaccines[J].Foreign Medical Sciences(Section of Bilogics for Prophylaxis,Diagnosis and Therapy),2003,26(1):21-30.
[17]PETERSON T S,SPITSBERGEN J M,FEIST S W,et al.Luna stain,an improved selective stain for detection of microsporidian spores in histologic sections[J].Diseases of Aquatic Organisms,2011,95(2):175-180.
[18]MOTA I.Passive cutaneous anaphylaxis induced with mast cell sensitizing antibody.The role of histamine and5-hydroxytryptamine[J].Life Sciences,1963,2(12):917-927.
[19]江英,席克虎,陈小婉,等.18β-甘草次酸对变应性鼻炎大鼠鼻黏膜纤毛超微结构的影响[J].第二军医大学学报,2005,36(1):26-33.JIANG Y,XI K H,CHEN X W,et al.Effect of 18β-glycyrrhetinic acid on ultrastructure of nasal mucosa cilia in rat models of allergic rhinitis[J].Academic Journal of Second Military Medical University,2005,36(1):26-33.
[20]Joo Hyun Jung,Il Gyu Kang,Dae Young Kim,et al.The effect of Korean red ginseng on allergic inflammation in a murine model of allergic[J].Journal of Ginseng Research,2013,37(2):167-175.
[21]Xiaoli Zhao,Shuangliu Liu,Zhi Tang,et al.Synthesis of magnetic metal-organic framework(MOF)for efficient removal of organic dyes from water[J].Scientific Reports,2015,5:10.
[22]BROIDE D H.Allergic rhinitis:Pathophysiology[J].Allergy and Asthma Proceedings,2010,31(5):370-374.
[23]MAJGAARD JENSEN O,KOCH C.On the effect of Al(OH)3as an immunological adjuvant[J].APMIS,1988,96(3):257-264.
[24]湛琳丽,温昊,祝明,等.铝佐剂的特性及影响因素[J].畜牧市场,2009,36(1):26-33.CHEN L L,WEN H,ZHU M,et al.Characteristics and influencing factors of aluminum adjuvant[J].Stockbreeding market,2009,36(1):26-33.
[25]李连奋.免疫佐剂的发展概况[J].微生物学免疫学进展,1997,25(2):57-61.LI LIAN F.An overview of the development of immune adjuvant[J].Progress In Microbiology and Immunology,1997,25(2):57-61.
[26]周柳青,刘勇强,张小兵.建立符合人类诊断标准的变应性鼻炎大鼠模型初探[J].甘肃科技,2016,36(22):127-128.ZHOU L Q,LIU Y Q,ZHANG X B.Establishment of a rat model of allergic rhinitis in accordance with the diagnostic criteria[J].Gansu Science and Technology,2016,36(22):127-128.
[27]BROIDE D H.Allergic rhinitis:Pathophysiology[J].Allergy and Asthma Proceedings,2010,31(5):370-374.
[28]中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组,中华医学会耳鼻咽喉头颈外科分会鼻科学组.变应性鼻炎诊断和治疗指南(2015年,天津)[J].中华耳鼻咽喉头颈外科杂志,2016,51(1):6-24.DEPARTMENT OF OTOLARYNGOLOGY EDITORIAL BOARD OF CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY,DEPARTMENT OF OTORHINOLARYNGOLOGY CHINESE MEDICAL ASSOCIATION DEPARTMENT OF OTOLARYNGOLOGY HEAD AND NECK SURGERY.Guidelines for diagnosis and treatment of allergic rhinitis(2015,Tianjin)[J].Chinese Journal of Otorhinolaryngology Head and Neck Surgery,2016,51(1):6-24.
[29]KIMIHIRO OKUBO,YUICHI KURONO,SHIGEHARU FUJIEDA,et al.Japanese Guideline for Allergic Rhinitis[J].Allergology International,2011,60(2):171-189.
[30]BAUCHAU V,DURHAM S R.Prevalence and rate of diagnosis of allergic rhinitis in Europe[J].European Respiratory Journal,2004,24(5):758-764.
[31]MEHMET OZGUR AVINCSAL,SEDA OZBAL,AHMET OMER IKIZ,et al.Effects of Topical Intranasal Doxycycline Treatment in the Rat Allergic Rhinitis Model[J].Clinical and Experimental Otorhinolaryngology,2014,7(2):106-111.
[32]D'AMATO G,CECCHI L,BONINI S,et al.Allergenic pollen and pollen allergy in Europe[J].Allergy,2007,62(9):976-990.
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