2016年云南省哨点医院标本流感病毒流行病学调查及血凝素基因特征分析
|
摘要
了解2016年度云南省哨点医院标本流感病毒的流行情况及其血凝素基因分子特征。对哨点医院采集的22 514份标本使用real-time RT-PCR方法筛选阳性,MDCK细胞进行病毒分离,SPSS软件进行统计分析。选取其中各亚型流感病毒共43株进行基因测序,使用MEGA5.0软件分析其血凝素基因特征。2016年共检出阳性标本1 310份(阳性率为5.82%),全年有两个流行高峰。甲型H1N1亚型流感病毒属于6B.2和6B.1分支;H3亚型流感病毒主要为3C.2a分支;Bv型流感病毒为1A和1B支系,By型则均属于Yamagata 3支系。部分毒株发生糖基化位点数量改变。2016年云南省流感病毒主要以Bv亚型为主,By、H3与甲型H1N1共流行的流行特点。各亚型流感病毒HA基因未发生明显变异,疫苗株仍具有保护作用。应继续加强流感监测,控制流感流行风险。
To investigate the epidemiological characteristics of influenza viruses and their hemagglutinin(HA)genes in Yunnan Province,China,2016.A total of 22,514 specimens,were collected from the sentinel hospitals in Yunnan Province,in 2016.Positive samples were verified using real-time reverse transcription-polymerase chain reaction.Virus isolation was performed with MDCK cells.Forty-three strains of influenza viruses strains were selected randomly and sequenced.The characteristics of HA genes were analyzed by MEGA5.0.A total of 1,310 positive samples were detected in 2016,with two epidemic peaks throughout the year;the positive rate was 5.82%.A(H1 N1)pdm09 divided into 6 B.2 and 6 B.1 subclades.Most H3 influenza viruses belonged to the 3 C.2 asubclade.All By strains were Yamagata 3 clade,wheras Bv strains were classified as 1 Aand 1 Bsubclades.The number of glycosylation sites changed in some strains.In 2016,there were two peaks in Yunnan Province of China,mainly caused by Bv subtype,as well as By,H3 and A(H1 N1)pdm virus.There was no obvious variation in HA genes of each subtype of influenza virus.Vaccine strain still maintained protective effect.Influenza surveillance should be strengthened to control the risk of influenza pandemic.
To investigate the epidemiological characteristics of influenza viruses and their hemagglutinin(HA)genes in Yunnan Province,China,2016.A total of 22,514 specimens,were collected from the sentinel hospitals in Yunnan Province,in 2016.Positive samples were verified using real-time reverse transcription-polymerase chain reaction.Virus isolation was performed with MDCK cells.Forty-three strains of influenza viruses strains were selected randomly and sequenced.The characteristics of HA genes were analyzed by MEGA5.0.A total of 1,310 positive samples were detected in 2016,with two epidemic peaks throughout the year;the positive rate was 5.82%.A(H1 N1)pdm09 divided into 6 B.2 and 6 B.1 subclades.Most H3 influenza viruses belonged to the 3 C.2 asubclade.All By strains were Yamagata 3 clade,wheras Bv strains were classified as 1 Aand 1 Bsubclades.The number of glycosylation sites changed in some strains.In 2016,there were two peaks in Yunnan Province of China,mainly caused by Bv subtype,as well as By,H3 and A(H1 N1)pdm virus.There was no obvious variation in HA genes of each subtype of influenza virus.Vaccine strain still maintained protective effect.Influenza surveillance should be strengthened to control the risk of influenza pandemic.
引文
[1]Peteranderl C,Herold S,Schmoldt C.Human influenza virus infections[J].Semin Resp Crit Care,2016,37(4):487.
[2]郭元吉,程小蠊.流行性感冒病毒及其实验技术[M].中国三峡出版社,1997.
[3]World Health Organization.Recommended composition of influenza virus vaccines for use in the 2017-2018northern hemisphere influenza season[EB/OL].http://www.who.int/influenza/vaccines/virus/recommendations/201703_recommendation.pdf?ua=1.
[4]Molinari N A,Ortegasanchez I R,Messonnier M L,Thompson W W,Wortley P M,Weintraub E,Bridges C B.The annual impact of seasonal influenza in the US:measuring disease burden and costs[J].Vaccine,2007,25(27):5086-5096.
[5]Yang P,Duan W,Lv M,Shi W,Peng X,Wang X,Lu Y,Liang H,Seale H,Pang X,Wang Q.Review of an influenza surveillance system,Beijing,People's Republic of China[J].Emerg Infect Dis,2009,15(10):1603.
[6]罗春蕊,李娟,宁德明,赵晓楠,徐闻.云南省2009-2013年流感哨点医院监测结果分析[J].实用预防医学,2016,23(3):358-360.
[7]中国疾病预防控制中心.全国流感监测技术指南(2010版)[Z].北京:中国疾病预防控制中心,2011.
[8]Puzelli S,Facchini M,Spagnolo D,Marco M A,Calzoletti L,Zanetti A,Fumagalli R,Tanzi M L,Cassone A,Rezza G,Donatelli I.Transmission of hemagglutinin D222G mutant strain of pandemic(H1N1)2009virus[J].Emerg Infect Dis,2010,16(5):863-865.
[9]黄维娟,董婕,舒跃龙.中国流感监测网络发展概况[J].疾病监测,2008,23(8):463-463.
[10]孙初阳,吴双志,刘磊,孙宇,吕昌武.3种流感病毒检测方法的比较[J].医学动物防制,2016(5):583-585.
[11]李娟,赵晓南,曹艺会,宁德明,伏晓庆,徐闻.云南省2009-2014年甲型H1N1流感病毒HA及NA基因特征分析[J].病毒学报,2015,31(6)674-678.
[12]王伟,姚巧玲.2012-2015年洛阳市流感监测结果分析[J].河南预防医学杂志,2017,28(8):617-620.
[13]刘玲,任斌知,那丹宇,王乃昌,赵嵘,王靖,陈利民.山西省2011-2016年流感病原学监测结果分析[J].国际病毒学杂志,2017,24(2):95-98.
[14]Friedman N,Drori Y,Pando R,Freedman A,Sefty H,Bassal R,Stein Y,Shohat T,Mendelson E,Hindiyeh M,Mandelboim M.A(H1N1)pdm09influenza infection:vaccine inefficiency[J].Oncotarget,2017,8(20):32856.
[15]梁丽君,倪汉忠,张欣,曾宪鍫,邹丽容,李来庆,宋颖超,黄平,武婕.2011-2015年广东省H3N2亚型流感病毒血凝素基因变异和进化分析[J].中华微生物学和免疫学杂志,2015,35(12):900-904.
[16]Tewawong N,Suntronwong N,Korkong S,Theamboonlers A,Vongpunsawad S,Poovorawan Y.Evidence for influenza B virus lineage shifts and reassortants circulating in Thailand in 2014-2016[J].Infect Genet Evol,2017,47:35-40.
[17]张晓剑,李彦芳,熊丽平,陈素娟,彭大新,刘秀梵.H5N1亚型禽流感病毒HA糖基化位点缺失突变株的构建及生物学特性[J].病毒学报,2013,29(5):495-499.
[18]Zhang X,Chen S,Jiang Y,Huang K,Huang J,Yang D,Zhu J,Zhu Y,Shi S,Peng D,Liu X.Hemagglutinin glycosylation modulates the pathogenicity and antigenicity of the H5N1avian influenza virus[J].Vet Microbiol,2015,175(2-4):244-256.
[19]Shen Y Y,Ke C W,Li Q,Yuan R Y,Xiang D,Jia W X,Yu Y D,Liu L,Huang C,Qi W B,Sikkema R,Wu J,Koopmans M,Liao M.Novel reassortant avian influenza A(H5N6)viruses in Humans,Guangdong,China,2015[J].Emerg Infect Dis,2016,22(8):1507-1509.
[20]Huang Y,Li X,Zhang H,Chen B,Jiang Y,Yang L,Zhu W,Hu S,Zhou S,Tang Y,Xiang X,Li F,Li W,Gao L.Human infection with an avian influenza A(H9N2)virus in the middle region of China[J].J Med Virol,2015,87(10):1641-1648.
[2]郭元吉,程小蠊.流行性感冒病毒及其实验技术[M].中国三峡出版社,1997.
[3]World Health Organization.Recommended composition of influenza virus vaccines for use in the 2017-2018northern hemisphere influenza season[EB/OL].http://www.who.int/influenza/vaccines/virus/recommendations/201703_recommendation.pdf?ua=1.
[4]Molinari N A,Ortegasanchez I R,Messonnier M L,Thompson W W,Wortley P M,Weintraub E,Bridges C B.The annual impact of seasonal influenza in the US:measuring disease burden and costs[J].Vaccine,2007,25(27):5086-5096.
[5]Yang P,Duan W,Lv M,Shi W,Peng X,Wang X,Lu Y,Liang H,Seale H,Pang X,Wang Q.Review of an influenza surveillance system,Beijing,People's Republic of China[J].Emerg Infect Dis,2009,15(10):1603.
[6]罗春蕊,李娟,宁德明,赵晓楠,徐闻.云南省2009-2013年流感哨点医院监测结果分析[J].实用预防医学,2016,23(3):358-360.
[7]中国疾病预防控制中心.全国流感监测技术指南(2010版)[Z].北京:中国疾病预防控制中心,2011.
[8]Puzelli S,Facchini M,Spagnolo D,Marco M A,Calzoletti L,Zanetti A,Fumagalli R,Tanzi M L,Cassone A,Rezza G,Donatelli I.Transmission of hemagglutinin D222G mutant strain of pandemic(H1N1)2009virus[J].Emerg Infect Dis,2010,16(5):863-865.
[9]黄维娟,董婕,舒跃龙.中国流感监测网络发展概况[J].疾病监测,2008,23(8):463-463.
[10]孙初阳,吴双志,刘磊,孙宇,吕昌武.3种流感病毒检测方法的比较[J].医学动物防制,2016(5):583-585.
[11]李娟,赵晓南,曹艺会,宁德明,伏晓庆,徐闻.云南省2009-2014年甲型H1N1流感病毒HA及NA基因特征分析[J].病毒学报,2015,31(6)674-678.
[12]王伟,姚巧玲.2012-2015年洛阳市流感监测结果分析[J].河南预防医学杂志,2017,28(8):617-620.
[13]刘玲,任斌知,那丹宇,王乃昌,赵嵘,王靖,陈利民.山西省2011-2016年流感病原学监测结果分析[J].国际病毒学杂志,2017,24(2):95-98.
[14]Friedman N,Drori Y,Pando R,Freedman A,Sefty H,Bassal R,Stein Y,Shohat T,Mendelson E,Hindiyeh M,Mandelboim M.A(H1N1)pdm09influenza infection:vaccine inefficiency[J].Oncotarget,2017,8(20):32856.
[15]梁丽君,倪汉忠,张欣,曾宪鍫,邹丽容,李来庆,宋颖超,黄平,武婕.2011-2015年广东省H3N2亚型流感病毒血凝素基因变异和进化分析[J].中华微生物学和免疫学杂志,2015,35(12):900-904.
[16]Tewawong N,Suntronwong N,Korkong S,Theamboonlers A,Vongpunsawad S,Poovorawan Y.Evidence for influenza B virus lineage shifts and reassortants circulating in Thailand in 2014-2016[J].Infect Genet Evol,2017,47:35-40.
[17]张晓剑,李彦芳,熊丽平,陈素娟,彭大新,刘秀梵.H5N1亚型禽流感病毒HA糖基化位点缺失突变株的构建及生物学特性[J].病毒学报,2013,29(5):495-499.
[18]Zhang X,Chen S,Jiang Y,Huang K,Huang J,Yang D,Zhu J,Zhu Y,Shi S,Peng D,Liu X.Hemagglutinin glycosylation modulates the pathogenicity and antigenicity of the H5N1avian influenza virus[J].Vet Microbiol,2015,175(2-4):244-256.
[19]Shen Y Y,Ke C W,Li Q,Yuan R Y,Xiang D,Jia W X,Yu Y D,Liu L,Huang C,Qi W B,Sikkema R,Wu J,Koopmans M,Liao M.Novel reassortant avian influenza A(H5N6)viruses in Humans,Guangdong,China,2015[J].Emerg Infect Dis,2016,22(8):1507-1509.
[20]Huang Y,Li X,Zhang H,Chen B,Jiang Y,Yang L,Zhu W,Hu S,Zhou S,Tang Y,Xiang X,Li F,Li W,Gao L.Human infection with an avian influenza A(H9N2)virus in the middle region of China[J].J Med Virol,2015,87(10):1641-1648.