中药木鳖子促进乳腺癌细胞ZR-75-30自噬研究
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摘要
本实验通过四甲基偶氮唑盐微量酶反应比色法(MTT法)、集落形成实验观察中药木鳖子对人乳腺癌细胞ZR-75-30的生长抑制作用,通过荧光染色(MDC)、流式细胞术和免疫印迹技术考察木鳖子能否促进细胞自噬的发生。结果表明,木鳖子水提取物(CMSWE)对乳腺癌细胞ZR-75-30的增殖具有明显的抑制作用;随着药物浓度的增大,发生自噬的细胞数目增加;Western杂交结果显示,随给药浓度的增加,自噬相关蛋白BECN1含量增加和LC3Ⅱ/LC3Ⅰ比值增大。以上结果证实木鳖子能促进乳腺癌细胞ZR-75-30发生自噬。
In this experiment, MTT assay and colony formation method were used to observe the growth inhibition effect of Chinese traditional medicineMomordica cochinchinensis(CMSWE) on human breast cancer cell line ZR-75-30. The results of fluorescent staining(MDC), flow cytometry and immunoblotting were used to study whether hibiscus can promote autophagy in cells. The results showed that CMSWE significantly inhibited the proliferation of breast cancer cell line ZR-75-30; the number of autophagic cells increased with increasing drug concentration; Western Blot showed that phage-associated protein increased with increasing drug concentration The content of BECN1 increased and the proportion of LC3 II/LC3 I increased.The above results confirmed that hibiscus can promote autophagy of the breast cancer cell line ZR-75-30.
In this experiment, MTT assay and colony formation method were used to observe the growth inhibition effect of Chinese traditional medicineMomordica cochinchinensis(CMSWE) on human breast cancer cell line ZR-75-30. The results of fluorescent staining(MDC), flow cytometry and immunoblotting were used to study whether hibiscus can promote autophagy in cells. The results showed that CMSWE significantly inhibited the proliferation of breast cancer cell line ZR-75-30; the number of autophagic cells increased with increasing drug concentration; Western Blot showed that phage-associated protein increased with increasing drug concentration The content of BECN1 increased and the proportion of LC3 II/LC3 I increased.The above results confirmed that hibiscus can promote autophagy of the breast cancer cell line ZR-75-30.
引文
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[2]领小,博·格日勒图,苏日娜.木鳖子研究进展[J].中药材,2007,9(2):56-57.
[3]刘涛,石军飞,吴晓忠.蒙药木鳖子的化学成分研究[J].内蒙古医学院学报,2010,31(4):390-393.
[4]王铭章,陈执中.木鳖子及其活性成分的研究进展[J].食品与药品,2011,13(9):364-365.
[5]范戎,许敏,马晓霞,等.苦瓜属植物的化学成分与药理活性研究进展[J].云南中医学院学报,2014,37(6):93-100.
[6]王铭章,陈执中.木鳖子及其活性成分的研究进展[J].食品与药品,2011,13(9):364-365.
[7]陈曦,梁文颖,熊静波.自噬的检测方法及评述[J].现代生物医学进展,2012,12(9):1797-1800.
[8]石峰,王明荣.细胞自噬及其与肿瘤关系的研究进展[J].中国细胞生物学学报,2011(12):1366-1373.
[9]王宠,张萍,朱卫国.细胞自噬与肿瘤发生的关系[J].中国生物化学与分子生物学报,2010,26(11):988-997.
[10]佳娜提·达吾列提,姜楠,寇俊萍,等.调节细胞自噬的中药有效成分研究进展[J].中草药,2014,45(16):2283-2292.