茉莉酸甲酯对铜藻生长、抗氧化系统及岩藻黄素含量的影响
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摘要
为了探讨茉莉酸甲酯(MeJA)对铜藻(Sargassum horneri)生长的影响作用,本文比较了不同浓度的茉莉酸甲酯对铜藻生长、生理生化指标、抗氧化系统以及次生代谢产物岩藻黄素积累的影响。结果发现,50和100μmol·L~(-1) MeJA处理后培养3 d显著促进了藻体的生长,继续培养至10 d时,与对照组无显著差异;而500μmol·L~(-1) MeJA处理后10 d内均显著抑制了藻体的生长。生理生化结果分析表明,培养3 d内,50～200μmol·L~(-1)MeJA处理可显著提高藻体叶绿素a和类胡萝卜素的含量,SOD、CAT和POD酶活性增强,而可溶性蛋白的含量变化不大;500μmol·L~(-1) MeJA处理组叶绿素a、类胡萝卜素和可溶性蛋白的含量均呈先升高后降低再升高的趋势,藻体SOD和POD酶活性增强,CAT酶活性降低;藻体MDA含量在50和100μmol·L~(-1) MeJA处理组无显著变化,200μmol·L~(-1) MeJA处理组显著低于对照组,而500μmol·L~(-1)处理组显著升高。MeJA处理后培养至10 d时,仅500μmol·L~(-1) MeJA处理组藻体的叶绿素a、可溶性蛋白含量、SOD和CAT活性高于对照,其余指标基本恢复至对照组水平。岩藻黄素含量在500μmol·L~(-1)MeJA处理后培养2和3 d时比对照组增加了12.55%和18.17%。这些结果为探讨MeJA在铜藻生长及岩藻黄素的规模化生产中的作用提供基础材料。
To explore the effects of methyl jasmonate(MeJA)on the growth of Sargassum horneri,the relative growth rate(SGR),physiological and biochemical indexes,antioxidant system and the accumulation of fucoxanthin were compared respectively while the thalli were treated with different concentrations of MeJA.The results showed that after treatments with 50 and 100μmol·L~(-1) MeJA,the algal growth was significantly promoted compared to control at 3 days,but there was no significant difference for further culture to 10 days.However,500μmol·L~(-1) MeJA significantly inhibited the growth of algae within 10 days.Physiological and biochemical analysis results revealed that within 3 days the chlorophyll a and carotenoid contents,SOD,CAT and POD activities were significantly increased when treated with 50-200μmol·L~(-1) MeJA,but the content of soluble protein had no variations.In 500μmol·L~(-1) treatment,the contents of chlorophyll a,carotenoid and soluble protein increased first,then decreased,after that it also increased,while the activities of SOD and POD improved,the CAT decreased.MDA contents in 50 and 100μmol·L~(-1) treatments were no significant variations,while in 200μmol·L~(-1) treatment was slightly lower than control,yet in 500 mol·L~(-1) treatment was markedly elevated than control.After 10 days culture,the contents of chlorophyll a,soluble protein,SOD and CAT activities were still higher than control in 500μmol·L~(-1) treatment,but the other indexes returned to the level of the control.The fucoxanthin content increased by 12.55% and 18.17%compared to control after treated with 500μmol·L~(-1) MeJA for 2 and 3 days.These findings provide essential materials for the impacts of MeJA on algal growth and the large-scale production of fucoxanthin of Sargassum horneri.
To explore the effects of methyl jasmonate(MeJA)on the growth of Sargassum horneri,the relative growth rate(SGR),physiological and biochemical indexes,antioxidant system and the accumulation of fucoxanthin were compared respectively while the thalli were treated with different concentrations of MeJA.The results showed that after treatments with 50 and 100μmol·L~(-1) MeJA,the algal growth was significantly promoted compared to control at 3 days,but there was no significant difference for further culture to 10 days.However,500μmol·L~(-1) MeJA significantly inhibited the growth of algae within 10 days.Physiological and biochemical analysis results revealed that within 3 days the chlorophyll a and carotenoid contents,SOD,CAT and POD activities were significantly increased when treated with 50-200μmol·L~(-1) MeJA,but the content of soluble protein had no variations.In 500μmol·L~(-1) treatment,the contents of chlorophyll a,carotenoid and soluble protein increased first,then decreased,after that it also increased,while the activities of SOD and POD improved,the CAT decreased.MDA contents in 50 and 100μmol·L~(-1) treatments were no significant variations,while in 200μmol·L~(-1) treatment was slightly lower than control,yet in 500 mol·L~(-1) treatment was markedly elevated than control.After 10 days culture,the contents of chlorophyll a,soluble protein,SOD and CAT activities were still higher than control in 500μmol·L~(-1) treatment,but the other indexes returned to the level of the control.The fucoxanthin content increased by 12.55% and 18.17%compared to control after treated with 500μmol·L~(-1) MeJA for 2 and 3 days.These findings provide essential materials for the impacts of MeJA on algal growth and the large-scale production of fucoxanthin of Sargassum horneri.
引文
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Raman V,Ravi S(2011).Effect of salicylic acid and methyl jasmonate on antioxidant systems of Haematococcus pluvialis.Acta Physiol Plant,33(3):1043-1049
Smetacek V,Zingone A(2013).Green and golden seaweed tides on the rise.Nature,504(7478):84-88
Wang XW,Wang LL,Gong YF,et al(2011).The effects of methyl jasmonate(MeJA)on the astaxanthin production and dxs gene expression of Haematococcus pluvialis.JFish China,35(12):1822-1828(in Chinese with English abstract)[王鑫威,王丽丽,龚一富等(2011).茉莉酸甲酯对雨生红球藻虾青素含量和dxs基因表达的影响.水产学报,35(12):1822-1828]
Wasternack C,Hause B(2013).Jasmonates:biosynthesis,perception,signal transduction and action in plant stress response,growth and development.An update to the 2007review in Annals of Botany.Ann Bot,111(6):1021-1058
Yan XY,Liu YX,Wu YP,et al(2014).Optimizing the processes of extracting and purifying fucoxanthin from Laminaria japonica.J Chin Inst Food Sci Tech,14(3):115-121(in Chinese with English abstract)[闫相勇,刘翼翔,吴永沛等(2014).海带岩藻黄素的提取及纯化工艺研究.中国食品学报,14(3):115-121]
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Yu LJ,Zhu M,Liu XF,et al(1999).Effect of methyl jasmonate on taxol biosynthesis of embryo cell line in Taxus chinensis.J Wuhan Bot Res,17(4):371-374(in Chinese with English abstract)[余龙江,朱敏,刘幸福等(1999).茉莉酸甲酯对中国红豆杉胚性细胞紫杉醇生物合成的影响.武汉植物学研究,17(4):371-374]
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Cai KZ,Dong TX,Xu T(2006).The physiological roles and resistance control in stress environment of jasmonates.Ecol Environ,15(2):397-404(in Chinese with English abstract)[蔡昆争,董桃杏,徐涛(2006).茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用.生态环境学报,15(2):397-404]
Chen HH,Qian KX(1997).The effect of nitrogen and phosphorus on the growth andβ-carotene accumulation of Dunaliella salina.J Zhejiang Univ(Nat Sci),31(6):731-736(in Chinese with English abstract)[陈晗华,钱凯先(1997).氮、磷对盐藻生长及其β-胡萝卜素积累的影响.浙江大学学报(自然科学版),31(6):731-736]
Chen JY,Gong YF,Ma YR,et al(2017).Bioinformatic analysis and induced expression of isopentenyl diphosphate isomerase gene(DvIPI)from Dunaliella viridis.Life Sci Res,21(3):201-207(in Chinese with English abstract)[陈俊粤,龚一富,马颖瑞等(2017).绿色杜氏藻异戊烯基焦磷酸异构酶基因(DvIPI)的生物信息学与诱导表达分析.生命科学研究,21(3):201-207]
Chen Y,Weng LQ,Zheng CL,et al(2018).Effects of plant growth regulators on galls formation of Zizania latifolia.Plant Physiol J,54(10):1605-1614(in Chinese with English abstract)[陈玥,翁丽青,郑春龙等(2018).植物生长调节剂对茭白孕茭的作用研究.植物生理学报,54(10):1605-1614]
Dummermuth AL,Karsten U,Fisch KM,et al(2003).Responses of marine macroalgae to hydrogen-peroxide stress.J Exp Mar Biol Ecol,289(1):103-121
Gundlach H,Müller MJ,Kutchan TM,et al(1992).Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures.Proc Natl Acad Sci USA,89(6):2389-2393
Hirata T,Tanaka J,Iwami T,et al(2003).Ecological studies on the community of drifting seaweeds in the south-eastern coastal waters of Izu Peninsula,central Japan.II:Seasonal changes in plants showing maximum stipe length in drifting seaweed communities.Phycol Res,51(3):186-191
Hou SN,Zou TL,Wang FJ,et al(2017).Effects of methyl jasmonate and salicylic acid on the growth and stress resistance of Pyropia haitanensis.Mar Sci,41(1):104-112(in Chinese with English abstract)[侯赛男,邹同雷,汪芳俊等(2017).茉莉酸甲酯和水杨酸对坛紫菜生长与抗逆性的影响.海洋科学,41(1):104-112]
Huang BX,Ding LP,Qin S,et al(2018).The taxonomical status and biogeographical distribution of Sargassum horneri with the origin analysis of its drifting population in the end of 2016 at the western Yellow Sea.Oceanol Limnol Sin,49(1):214-223(in Chinese with English abstract)[黄冰心,丁兰平,秦松等(2018).铜藻的分类地位、生物地理分布以及2016年底黄海漂浮铜藻源头的初步分析.海洋与湖沼,49(1):214-223]
Kang SM,Min JY,Kim YD,et al(2006).Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba.In Vitro Cell Dev Biol:Plant,42(1):44-49
Li HY,Liu TH,Jiang XD,et al(2018).Optimization of ultrasonic enhanced extraction of fucoxanthin from Sargassum horneri.J Agr Sci Tech,20(9):146-153(in Chinese with English abstract)[李红艳,刘天红,姜晓东等(2018).超声辅助乙醇提取铜藻中岩藻黄素的工艺研究.中国农业科技导报,20(9):146-153]
Lu N,Zang XN,Zhang XC,et al(2012).Effects of stress on antioxidant enzyme system in algae.J Wuhan Univ(Nat Sci Ed),58(2):119-124(in Chinese with English abstract)[鹿宁,臧晓南,张学成等(2012).逆境胁迫对藻类抗氧化酶系统的影响.武汉大学学报(理学版),58(2):119-124]
Luan Q(2018).Effects of different environment and processing conditions on the growth and nutrient components of Phaeophyta(dissertation).Yantai,Shandong:Yantai University(in Chinese with English abstract)[栾青(2018).不同环境条件和加工条件对经济褐藻生长及营养组成的影响(学位论文).山东烟台:烟台大学]
LüF,Ding G,Wu HY,et al(2018b).Effects of uniconazole on growth of antioxidation system in Sargassum horneri.Oceanol Limnol Sin,49(2):361-367(in Chinese with English abstract)[吕芳,丁刚,吴海一等(2018b).烯效唑对铜藻(Sargassum horneri)生长及抗氧化系统的影响.海洋与湖沼,49(2):361-367]
LüF,Guo W,Ding G,et al(2018a).Analysis of the similarity of ITS and cox I sequences of Sargassum horneri along the offshore of China.Prog Fish Sci,39(6):141-146(in Chinese with English abstract)[吕芳,郭文,丁刚等(2018a).中国近海铜藻ITS与cox I序列相似性分析.渔业科学进展,39(6):141-146]
LüF,Wu HY,Ding G,et al(2019).Effects of triacontanol on the growth and fucoxanthin content of Sargassum horneri.J Fish China,43(4):1048-1057(in Chinese with English abstract)[吕芳,吴海一,丁刚等(2019).三十烷醇对铜藻生长及岩藻黄素含量的影响.水产学报,43(4):1048-1057
Pan YF,Gong YF,Yu K,et al(2015).Bioinformatics analysis and expression study of DHDDS of Dunaliella viridis.Biotechnol Bull,31(7):101-108(in Chinese with English abstract)[潘益芳,龚一富,俞凯等(2015).绿色杜氏藻DHDDS基因生物信息学分析及表达研究.生物技术通报,31(7):101-108]
Raman V,Ravi S(2011).Effect of salicylic acid and methyl jasmonate on antioxidant systems of Haematococcus pluvialis.Acta Physiol Plant,33(3):1043-1049
Smetacek V,Zingone A(2013).Green and golden seaweed tides on the rise.Nature,504(7478):84-88
Wang XW,Wang LL,Gong YF,et al(2011).The effects of methyl jasmonate(MeJA)on the astaxanthin production and dxs gene expression of Haematococcus pluvialis.JFish China,35(12):1822-1828(in Chinese with English abstract)[王鑫威,王丽丽,龚一富等(2011).茉莉酸甲酯对雨生红球藻虾青素含量和dxs基因表达的影响.水产学报,35(12):1822-1828]
Wasternack C,Hause B(2013).Jasmonates:biosynthesis,perception,signal transduction and action in plant stress response,growth and development.An update to the 2007review in Annals of Botany.Ann Bot,111(6):1021-1058
Yan XY,Liu YX,Wu YP,et al(2014).Optimizing the processes of extracting and purifying fucoxanthin from Laminaria japonica.J Chin Inst Food Sci Tech,14(3):115-121(in Chinese with English abstract)[闫相勇,刘翼翔,吴永沛等(2014).海带岩藻黄素的提取及纯化工艺研究.中国食品学报,14(3):115-121]
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