丁酸梭菌CB1对肉鸡免疫器官指数、黏膜SIgA抗体和血清生化指标的影响
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摘要
试验选取1日龄Cobb500肉鸡15 000只,随机分为5组,每组3 000只,每组3个重复,试验周期为42d,前期1~21d和后期22~42d。试验分组为基础日粮(A组)、基础日粮+50g/t金霉素(B组)、基础日粮+100g/t丁酸梭菌CB1制剂(C1组)、基础日粮+200g/t丁酸梭菌CB1制剂(C2组)、基础日粮+200g/t丁酸梭菌CB1复合菌制剂(D组),分别在21d和42d时每个重复随机抽取10只鸡进行屠宰取样,测定相关指标。结果显示:丁酸梭菌CB1制剂添加组C1、C2和D组42d法氏囊指数比A组、B组分别提高了347.37%,520%和400%(P<0.01),脾脏指数比B组显著提高了61.11%,46.67%和31.11%。C1、C2、D组气管黏膜SIgA水平显著高于A组和B组,C2组、D组肠道黏膜SIgA水平显著高于A组和B组。21d和42d,血糖含量C1、C2、D组与A、B组相比均有所提高,D组显著高于A和B组(P<0.05);尿素氮含量C1、C2、D组均低于A组和B组,D组显著低于A和B组(P<0.05)。21d,D组总胆固醇、肌酐也显著低于A和B组(P<0.05),但42d差异不显著。结果表明:丁酸梭菌CB1及其复合菌制剂能有效促进法氏囊和脾脏的生长发育,对于后期法氏囊的生长维持作用优于抗生素和基础日粮组;能有效刺激肉鸡气管和肠道体黏膜SIgA的分泌;在一定程度上改善了肉鸡血液生化指标,复合菌制剂组在血糖含量、尿素氮指标显著优于抗生素组。
This study was designed to investigate the effect of Clostridium butyricum CB1 preparation and composite probiotics added to the diets on broiler immune function and serum biochemical parameters.In this experiment,15 000 one-day old Cobb500 broiler chickens were randomly assigned to 5 treatments of 3 000 each, and each group has 3 duplication for 42 days.It was divided into the early stage(1-21 d) and the later stage(22-42 d).The treatments were as follows: basal diet tion(group C2),basal diet + composite probiotics of 200 g/t Clostridium butyricum CB1 preparation and Bacillus Subtilis preparation(group D). Weekly weighed to determine the production performance in 21 d and 42 d respectively,each set of 10 randomly selected chickens were slaughtered sampling and measure related indicators.The test results showed that at 42 d bursal indexes of broiler chickens group C1,C2 and D fed with Clostridium butyricum CB1 preparation improved347.37 %, 520 % and 400 %( P < 0. 01) respectively compared with the group A and group B,and spleen indexes improved 61.11 %,46.67 % and 31.11% compared with group B. Also, tracheal mucosa SIgA levels of broilers in group C1,C2,D were significantly higher than those of group A,B and intestine mucosa SIgA levels of broilers in group C2,D were higher than those of group A,B.At 21 d and 42 d,blood glucose content of group C1,C2,D increased to some extent compared with group A,B and group D were higher than group A,B(P<0.05).At 21 d,blood urea nitrogen and creatinine content of group D was obviously lower than group A,B(P<0.05),but without significant effects at 42 d.So conclusions were drawn that Clostridium butyricum CB1 preparation and composite probiotics could effectively facilitate the growth and development of bursa of fabricius and spleen,and growth and maintenance effect on the late bursa of fabricius was better than that of basal diet and hlortetracycline group.They could also effectively stimulus the secretion of mucous membrane SIgA in broiler trachea and intestinal tract as well as improved blood biochemical indexes in some occasions.Blood glucose and blood urea nitrogen parameters in Clostridium butyricum CB1 preparation and composite probiotics group were obviously superior to antibiotics group.
This study was designed to investigate the effect of Clostridium butyricum CB1 preparation and composite probiotics added to the diets on broiler immune function and serum biochemical parameters.In this experiment,15 000 one-day old Cobb500 broiler chickens were randomly assigned to 5 treatments of 3 000 each, and each group has 3 duplication for 42 days.It was divided into the early stage(1-21 d) and the later stage(22-42 d).The treatments were as follows: basal diet tion(group C2),basal diet + composite probiotics of 200 g/t Clostridium butyricum CB1 preparation and Bacillus Subtilis preparation(group D). Weekly weighed to determine the production performance in 21 d and 42 d respectively,each set of 10 randomly selected chickens were slaughtered sampling and measure related indicators.The test results showed that at 42 d bursal indexes of broiler chickens group C1,C2 and D fed with Clostridium butyricum CB1 preparation improved347.37 %, 520 % and 400 %( P < 0. 01) respectively compared with the group A and group B,and spleen indexes improved 61.11 %,46.67 % and 31.11% compared with group B. Also, tracheal mucosa SIgA levels of broilers in group C1,C2,D were significantly higher than those of group A,B and intestine mucosa SIgA levels of broilers in group C2,D were higher than those of group A,B.At 21 d and 42 d,blood glucose content of group C1,C2,D increased to some extent compared with group A,B and group D were higher than group A,B(P<0.05).At 21 d,blood urea nitrogen and creatinine content of group D was obviously lower than group A,B(P<0.05),but without significant effects at 42 d.So conclusions were drawn that Clostridium butyricum CB1 preparation and composite probiotics could effectively facilitate the growth and development of bursa of fabricius and spleen,and growth and maintenance effect on the late bursa of fabricius was better than that of basal diet and hlortetracycline group.They could also effectively stimulus the secretion of mucous membrane SIgA in broiler trachea and intestinal tract as well as improved blood biochemical indexes in some occasions.Blood glucose and blood urea nitrogen parameters in Clostridium butyricum CB1 preparation and composite probiotics group were obviously superior to antibiotics group.
引文
[1]李伟忠.合生素对肉仔鸡作用的研究[D].黑龙江哈尔滨:东北农业大学,2003.
[2]郝永任.新型动物微生态制剂—复合酪酸菌制剂的研制[D].山东济南:山东师范大学,2002.
[3]苗晓微.合生素对肉鸡生产性能的研究[D].吉林延吉:延边大学,2006.
[4]唐志刚,王俊峰,温超,等.益生菌对肉鸡生产性能、免疫器官指数和血清指标的影响[J].江苏农业科学,2010(4):208-210.
[5]刘亭婷,滑静,王晓霞,等.丁酸梭菌对蛋用仔公鸡生长性能、免疫指标及空肠组织相关细胞因子基因mRNA表达的影响[J].动物营养学报,2012,24(7):1302-1310.
[6]王俊峰,陈雁南,温超,等.合生素对肉鸡生长性能、免疫器官指数、血清免疫指标及肠道菌群的影响[J].动物营养学报,2010,22(1):163-168.
[7]ZHANG L,CAO G T,ZENG X F,et al.Effects of Clostridium butyricum cn growth performance,immune function,and cecal microflora in broiler chickens challenged with Escherichia coli K88[J].Poult Sci,2014,93:46-53.
[8]张和平,张七斤,孟和毕力格,等.L.casei Zhang对小鼠T淋巴细胞亚群及血清IgG和肠黏膜SIgA的影响[J].中国乳品工业,2006,34(10):4-8.
[9]王丽凤.益生菌L.plantarum P-8对肉鸡肠道菌群、肠道免疫和生长性能影响的研究[D].内蒙古呼和浩特:内蒙古农业大学,2014.
[10]何欣,滑静,王晓霞,等.酵母盐对肉仔鸡生产性能、胫骨质量及血液生化指标的影响[J].北京农学院学报,2004,19(4):34-36.
[11]李万军.酸化剂对肉鸡生长及免疫的影响[J].饲料研究,2012(4):75-77.
[12]杜晋平,王云方,杨待建.益生素对肉仔鸡免疫机能的影响[J].畜牧与饲料科学,2004,25(5):5-6.
[13]滑静,郭玉琴,张淑萍,等.肉仔鸡日粮中添加枯草芽孢杆菌对平均日增质量和血液生化指标的影响[J].黑龙江畜牧兽医,2003(2):14-15.
[2]郝永任.新型动物微生态制剂—复合酪酸菌制剂的研制[D].山东济南:山东师范大学,2002.
[3]苗晓微.合生素对肉鸡生产性能的研究[D].吉林延吉:延边大学,2006.
[4]唐志刚,王俊峰,温超,等.益生菌对肉鸡生产性能、免疫器官指数和血清指标的影响[J].江苏农业科学,2010(4):208-210.
[5]刘亭婷,滑静,王晓霞,等.丁酸梭菌对蛋用仔公鸡生长性能、免疫指标及空肠组织相关细胞因子基因mRNA表达的影响[J].动物营养学报,2012,24(7):1302-1310.
[6]王俊峰,陈雁南,温超,等.合生素对肉鸡生长性能、免疫器官指数、血清免疫指标及肠道菌群的影响[J].动物营养学报,2010,22(1):163-168.
[7]ZHANG L,CAO G T,ZENG X F,et al.Effects of Clostridium butyricum cn growth performance,immune function,and cecal microflora in broiler chickens challenged with Escherichia coli K88[J].Poult Sci,2014,93:46-53.
[8]张和平,张七斤,孟和毕力格,等.L.casei Zhang对小鼠T淋巴细胞亚群及血清IgG和肠黏膜SIgA的影响[J].中国乳品工业,2006,34(10):4-8.
[9]王丽凤.益生菌L.plantarum P-8对肉鸡肠道菌群、肠道免疫和生长性能影响的研究[D].内蒙古呼和浩特:内蒙古农业大学,2014.
[10]何欣,滑静,王晓霞,等.酵母盐对肉仔鸡生产性能、胫骨质量及血液生化指标的影响[J].北京农学院学报,2004,19(4):34-36.
[11]李万军.酸化剂对肉鸡生长及免疫的影响[J].饲料研究,2012(4):75-77.
[12]杜晋平,王云方,杨待建.益生素对肉仔鸡免疫机能的影响[J].畜牧与饲料科学,2004,25(5):5-6.
[13]滑静,郭玉琴,张淑萍,等.肉仔鸡日粮中添加枯草芽孢杆菌对平均日增质量和血液生化指标的影响[J].黑龙江畜牧兽医,2003(2):14-15.