PLCE1与MMP-9在口腔鳞癌中的表达及其临床意义
|
摘要
目的:探讨磷脂酶Cε1(PLCE1)与基质金属蛋白酶-9(MMP-9)在口腔鳞癌中的表达及临床意义。方法:采用免疫组化方法检测61例口腔鳞癌组织和35例正常口腔黏膜组织中PLCE1和MMP-9的蛋白表达,并分析二者与口腔鳞状细胞癌临床病理参数的关系及二者的相关性。结果:PLCE1在口腔鳞癌组织中表达阳性率为68.85%(42/61),MMP-9在口腔鳞癌组织中表达阳性率为75.40%(46/61),两者在正常口腔黏膜组织中表达阳性率分别为14.28%(5/35)、17.14%(6/35)。PLCE1和MMP-9在正常口腔黏膜组织的阳性表达率均明显低于口腔鳞癌组织(P<0.01)。PLCE1与MMP-9的高度阳性表达和患者的性别、年龄、吸烟及肿瘤大小无明显相关性,但与肿瘤TNM分期以及组织分化程度显著相关。口腔鳞癌组织中PLCE1和MMP-9的高表达呈明显正相关(r=0.438,P<0.01)。结论:PLCE1和MMP-9的过度表达均与口腔鳞癌的发生、发展及侵袭转移有密切相关性,并检测二者可能会对口腔鳞癌患者的早期临床诊断及预后判断具有一定的参考价值。
Objective: To study the expressions and clinical significances of phospholipase C epsilon-1(PLCE1) and matrix metalloproteinase-9(MMP-9) in the oral squamous cell carcinoma(OSCC). Methods: The expressions of PLCE1 and MMP-9 were detected by immunohistochemistry method in 61 cases of OSCC patients and 35 cases of controls with normal oral mucosa tissues. The relationship of PLCE1 and MMP-9 expressions with the clinicopathologic features were analyzed. Results: The postive expression rates of PLCE1 and MMP-9 in the OSCC(68.85 %, 75.40 %) were significantly higher than those of the normal oral mucosa tissue(14.28 %,17.14 %)(P <0.01). The expression of PLCE1 was positively correlated with the expression of MMP-9(r=0.438, P <0.01). The high expressions of PLCE1 and MMP-9 were associated with the differentiation degree and TNM stage of OSCC, but they had no correlation with the gender, age, smoker and tumor size of OSCC patients. Conclusions: The high expressions of PLCE1 and MMP-9 were closely related to the occurrence, development and metastasis of OSCC. Therefore, combined detection of PLCE1 and MMP-9 might be valuable for the early diagnosis and prognostic prediction of OSCC.
Objective: To study the expressions and clinical significances of phospholipase C epsilon-1(PLCE1) and matrix metalloproteinase-9(MMP-9) in the oral squamous cell carcinoma(OSCC). Methods: The expressions of PLCE1 and MMP-9 were detected by immunohistochemistry method in 61 cases of OSCC patients and 35 cases of controls with normal oral mucosa tissues. The relationship of PLCE1 and MMP-9 expressions with the clinicopathologic features were analyzed. Results: The postive expression rates of PLCE1 and MMP-9 in the OSCC(68.85 %, 75.40 %) were significantly higher than those of the normal oral mucosa tissue(14.28 %,17.14 %)(P <0.01). The expression of PLCE1 was positively correlated with the expression of MMP-9(r=0.438, P <0.01). The high expressions of PLCE1 and MMP-9 were associated with the differentiation degree and TNM stage of OSCC, but they had no correlation with the gender, age, smoker and tumor size of OSCC patients. Conclusions: The high expressions of PLCE1 and MMP-9 were closely related to the occurrence, development and metastasis of OSCC. Therefore, combined detection of PLCE1 and MMP-9 might be valuable for the early diagnosis and prognostic prediction of OSCC.
引文
[1] Wang Y, Feng SH, Zhu J, et al. Impect of Iymph node ratio on thesurvial of patients with hypopharyyngeal squamous cell carcinoma:Apouplation based analysis[J]. PLo S ONE, 20138(2):e56613
[2] Lee CC, Ho HC, Su YC, et al. The prognastic ability of log odds ofpositive lymph nodes in oralcavity squamous cell carcinoma[J].Medicine, 2015, 94(27):e1069
[3] Gang-cai Zhu, Chang-yun Yu, Li She, et al. Metadherin Regulation ofVascular Endothelial Growth Factor Expression Is Dependent Uponthe PI3K/Akt Pathy IN Squamous Cell Carcinoma of the Head andeck[J]. Medicin, 2015, 94(6):1-10
[4]李晓岑,王秀梅.磷脂酶Cε1与肿瘤相关性的研究进展[J].现代肿瘤医学, 2015, 23(21):3207-3210Li Xiao-cen, Wang Xiu-mei. Research of ohospholipase C epsilon-1in tumor[J]. Journal of Modern Oncology, 2015, 23(21):3207-3210
[5] Ma H, Wang LE, Liu z, et al. Association between novel PLCE1variants identified in published esophageal cancer genome-wideassociation studies and risk of squamous cell carcioma of the headneck[J]. BMC Cancer, 2011, 11:258
[6] SUNRongzeg, L Hanlin, Wang Xiao-chen. Clinical Significance ofExpression of Phosho-lipase C Epsilon-1 in Heptocelular Carcinoma,Breast Cancer and Lung Cancer[J]. Medi-cal Journal of WuhanUniversity, 2015, 12(4):1617-8852
[7] Lad Y, Mchugh B, Hodknson PS, et al. Phospholipase C epsilonsuppresses integrinacti activation[J]. J Biol Chem, 2006, 281(40):29501-29512
[8] Nascimento DD, Durigan RDM, Tibana RA, et al. The response ofmatrix metalloproteins-e-9 and-2 to exercies[J]. Sports Med, 2015,45(2):278-309
[9] Xiao-bin Cui, Xuelian Pang, Su Li, et al. Elevated expression pat ternsand tight correlation of the PLCE1 and NF-κB signaling in Kazakhpatients with esophageal carcinoma[J]. Med Oncol, 2014, 31:791-795
[10]张伟,方子乔,吉化春,等.结肠腺癌组织MTDH和MMP-9表达临床意义研究[J].中华肿瘤防治杂志,2015, 22(10):786-795Zhang Wei, Fang Zi-qiao, Ji Hua-chun, et al. Expression on andsignificance of MTDH and MMP-9 proteins in colon adenocarcinoma[J]. Chin J Cancer Prev Treat, 2015, 22(10):786-795
[11] Gresset A, Sondek J, Haeden T K. The phospholipase C isozymes andtheir regulation[J]. Sub-cellular biochemistry, 2012, 58:61-94
[12] Dusaban S S, Purcell N H, Edward R, et al. Phospholipase C epsilonG protien coupled recerptor activation to inf-lammatory astrocyticresponses[J]. Proceedings of the National Academy of Sciences, 2013,110(9):3609-3614
[13] Ferrandiz N, Caraballo JM, Garcia-Gutierrez L, et al. P21 as atranscriptional corepessor of S phase and mitotic control genes[J].PLo S One, 2012, 7:37759
[14]赵学科,周福有,张连群等,食管癌变过程中PLCE1的表达[J].河南大学学报(医学版), 2012, 31(3):203-205Zhao Xue-ke, Zhou Fu-you, Zhang Lian-qun, et al. Expression ofPLCE1 in esophageal carcinogenesis[J]. Journal of Henan University(Social Science), 2012, 31(3):203-205
[15] Liping Qu, Yongcan Guo, Chunli Luo, et al. Interference suppressingPLCE1 gene expression decrease invasive power of human bladdercancer T24 cell line[J]. Cancer Genetics and Cytogenetics, 2010, 200(2):110-119
[16] Garg R, Caino MC, Kazanietz MG. Regulation of TrascriptionalNetworks by PKC Isozymes:Identification of c-Rel as a KeyTranscription Factor for PKC-Regulated Genes[J]. PLoS One, 2013,8(6):e67319
[17]张伟,方子乔,吉化春,等.结肠腺癌组织MTDH和MMP-9表达临床意义研究[J].中华肿瘤防杂志, 2015, 22(10):786-795Zhang Wei, Fang Zi-qiao, Ji Hua-chun, et al. Expression andsignificance of MTDH and MMP-9 proteins in colon ad-enocarcinoma[J]. Chin J Cancer Prev Treat, 2015, 22(10):786-795
[18]黄炎,柯儒斌,徐新伟,等.MTDH在胰腺癌中的表达及其与E-cadherin、MVD的关系[J].肿瘤学杂志,2015, 21(6):475-481Huang Yan, Ke Ru-bin, Xv Xin-wei, et al. Expression of MTDH inPancreatic Carcinoma and Its Correlations with E-cadherin andMicrovascular Density[J]. Journal of Chinese Oncology, 2015, 21(6):475-481
[19]汤夏冰,沈晓辉,钱晓云,等.高迁移率族蛋白B1和基质金属蛋白酶-2、9在喉癌组织中的表达及其与预后的关系[J].临床耳鼻咽喉头颈外科杂志, 2013, 27(4):181-187Tang Xia-bing, Shen Xiao-hui, Qian Xiao-yun, et al. Expressions ofHMGB1, MMP-9 and prog nostic alue in human laryngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surg, 2013, 27(4):181-187
[20]陈丽,向丽,周铁军,等.口腔鳞癌中RECK和MMP-9蛋白的表达及临床意义[J].泸州医学院学报, 2014, 37(1):1000-2669Chen Li, Xia Li, Zhou Tie-jun, et al. The expression of RECK andMMP-9 protein in oral squamous cell carcinoma and its clinicalsignificance[J]. Journal of Lu Zhou Medical College, 2014, 37(1):1000-2669
[21] Cui XB, Li S, Li TT, et al. Targeting oncogenic PLCE1 by miR-145impairs tumor proliferation and metastssis of esophageal squamouscell carcinoma[J]. Oncatarget, 2015, 7(2):177-1795
[2] Lee CC, Ho HC, Su YC, et al. The prognastic ability of log odds ofpositive lymph nodes in oralcavity squamous cell carcinoma[J].Medicine, 2015, 94(27):e1069
[3] Gang-cai Zhu, Chang-yun Yu, Li She, et al. Metadherin Regulation ofVascular Endothelial Growth Factor Expression Is Dependent Uponthe PI3K/Akt Pathy IN Squamous Cell Carcinoma of the Head andeck[J]. Medicin, 2015, 94(6):1-10
[4]李晓岑,王秀梅.磷脂酶Cε1与肿瘤相关性的研究进展[J].现代肿瘤医学, 2015, 23(21):3207-3210Li Xiao-cen, Wang Xiu-mei. Research of ohospholipase C epsilon-1in tumor[J]. Journal of Modern Oncology, 2015, 23(21):3207-3210
[5] Ma H, Wang LE, Liu z, et al. Association between novel PLCE1variants identified in published esophageal cancer genome-wideassociation studies and risk of squamous cell carcioma of the headneck[J]. BMC Cancer, 2011, 11:258
[6] SUNRongzeg, L Hanlin, Wang Xiao-chen. Clinical Significance ofExpression of Phosho-lipase C Epsilon-1 in Heptocelular Carcinoma,Breast Cancer and Lung Cancer[J]. Medi-cal Journal of WuhanUniversity, 2015, 12(4):1617-8852
[7] Lad Y, Mchugh B, Hodknson PS, et al. Phospholipase C epsilonsuppresses integrinacti activation[J]. J Biol Chem, 2006, 281(40):29501-29512
[8] Nascimento DD, Durigan RDM, Tibana RA, et al. The response ofmatrix metalloproteins-e-9 and-2 to exercies[J]. Sports Med, 2015,45(2):278-309
[9] Xiao-bin Cui, Xuelian Pang, Su Li, et al. Elevated expression pat ternsand tight correlation of the PLCE1 and NF-κB signaling in Kazakhpatients with esophageal carcinoma[J]. Med Oncol, 2014, 31:791-795
[10]张伟,方子乔,吉化春,等.结肠腺癌组织MTDH和MMP-9表达临床意义研究[J].中华肿瘤防治杂志,2015, 22(10):786-795Zhang Wei, Fang Zi-qiao, Ji Hua-chun, et al. Expression on andsignificance of MTDH and MMP-9 proteins in colon adenocarcinoma[J]. Chin J Cancer Prev Treat, 2015, 22(10):786-795
[11] Gresset A, Sondek J, Haeden T K. The phospholipase C isozymes andtheir regulation[J]. Sub-cellular biochemistry, 2012, 58:61-94
[12] Dusaban S S, Purcell N H, Edward R, et al. Phospholipase C epsilonG protien coupled recerptor activation to inf-lammatory astrocyticresponses[J]. Proceedings of the National Academy of Sciences, 2013,110(9):3609-3614
[13] Ferrandiz N, Caraballo JM, Garcia-Gutierrez L, et al. P21 as atranscriptional corepessor of S phase and mitotic control genes[J].PLo S One, 2012, 7:37759
[14]赵学科,周福有,张连群等,食管癌变过程中PLCE1的表达[J].河南大学学报(医学版), 2012, 31(3):203-205Zhao Xue-ke, Zhou Fu-you, Zhang Lian-qun, et al. Expression ofPLCE1 in esophageal carcinogenesis[J]. Journal of Henan University(Social Science), 2012, 31(3):203-205
[15] Liping Qu, Yongcan Guo, Chunli Luo, et al. Interference suppressingPLCE1 gene expression decrease invasive power of human bladdercancer T24 cell line[J]. Cancer Genetics and Cytogenetics, 2010, 200(2):110-119
[16] Garg R, Caino MC, Kazanietz MG. Regulation of TrascriptionalNetworks by PKC Isozymes:Identification of c-Rel as a KeyTranscription Factor for PKC-Regulated Genes[J]. PLoS One, 2013,8(6):e67319
[17]张伟,方子乔,吉化春,等.结肠腺癌组织MTDH和MMP-9表达临床意义研究[J].中华肿瘤防杂志, 2015, 22(10):786-795Zhang Wei, Fang Zi-qiao, Ji Hua-chun, et al. Expression andsignificance of MTDH and MMP-9 proteins in colon ad-enocarcinoma[J]. Chin J Cancer Prev Treat, 2015, 22(10):786-795
[18]黄炎,柯儒斌,徐新伟,等.MTDH在胰腺癌中的表达及其与E-cadherin、MVD的关系[J].肿瘤学杂志,2015, 21(6):475-481Huang Yan, Ke Ru-bin, Xv Xin-wei, et al. Expression of MTDH inPancreatic Carcinoma and Its Correlations with E-cadherin andMicrovascular Density[J]. Journal of Chinese Oncology, 2015, 21(6):475-481
[19]汤夏冰,沈晓辉,钱晓云,等.高迁移率族蛋白B1和基质金属蛋白酶-2、9在喉癌组织中的表达及其与预后的关系[J].临床耳鼻咽喉头颈外科杂志, 2013, 27(4):181-187Tang Xia-bing, Shen Xiao-hui, Qian Xiao-yun, et al. Expressions ofHMGB1, MMP-9 and prog nostic alue in human laryngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surg, 2013, 27(4):181-187
[20]陈丽,向丽,周铁军,等.口腔鳞癌中RECK和MMP-9蛋白的表达及临床意义[J].泸州医学院学报, 2014, 37(1):1000-2669Chen Li, Xia Li, Zhou Tie-jun, et al. The expression of RECK andMMP-9 protein in oral squamous cell carcinoma and its clinicalsignificance[J]. Journal of Lu Zhou Medical College, 2014, 37(1):1000-2669
[21] Cui XB, Li S, Li TT, et al. Targeting oncogenic PLCE1 by miR-145impairs tumor proliferation and metastssis of esophageal squamouscell carcinoma[J]. Oncatarget, 2015, 7(2):177-1795