合欢磁牡口服液提取工艺研究
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摘要
为探究不同的提取条件对合欢磁牡口服液质量的影响,以确定最佳的提取工艺。通过不同的提取工艺、浓缩工艺及提取工艺验证试验处理后,检测合欢磁牡口服液中(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷(C_(33)H_(44)O_(17))的含量。结果显示,煎煮次数C对提取工艺有显著影响(P<0.01);煎煮时间B和加水量A对提取工艺无显著影响(P>0.05);不同的浓缩温度对合欢磁牡口服液含量无显著影响(P>0.05),采用本试验确定的最佳工艺提取的合欢磁牡口服液中C_(33)H_(44)O_(17)的转移率平均值为72.37%,RSD为0.83%。因此,确定的最佳提取生产工艺为8倍量水,提取3次,每次煮沸1 h。
The purpose of this experiment was to investigate the effect of different extraction conditions on the quality of Hehuancimu oral liquid to determine the best extraction technology.After different extraction,concentration and the verification tests of extraction technology,the content of(-)-eugenol-4-O-beta-D-furanin glycosyl-(1→2)-beta-D-pyran glucose(C_(33)H_(44)O_(17))in Hehuancimu oral liquid was determined.The results showed that the times of decoction(C)had a significant effect on the extraction technology(P<0.01);the time of decoction(B)and the amount of water added(A)had no significant effect on the extraction technology(P>0.05);and there was no significant difference in the effect of different concentration temperature on the content of Hehuancimu oral liquid(P>0.05);the average transfer rate of C_(33)H_(44)O_(17)extracted by the best extraction technology was 72.37%and RSD was 0.83%.Therefore,the optimal extraction production technology was determined as 8 times water,3 times extraction,1 hour boiling each time.
The purpose of this experiment was to investigate the effect of different extraction conditions on the quality of Hehuancimu oral liquid to determine the best extraction technology.After different extraction,concentration and the verification tests of extraction technology,the content of(-)-eugenol-4-O-beta-D-furanin glycosyl-(1→2)-beta-D-pyran glucose(C_(33)H_(44)O_(17))in Hehuancimu oral liquid was determined.The results showed that the times of decoction(C)had a significant effect on the extraction technology(P<0.01);the time of decoction(B)and the amount of water added(A)had no significant effect on the extraction technology(P>0.05);and there was no significant difference in the effect of different concentration temperature on the content of Hehuancimu oral liquid(P>0.05);the average transfer rate of C_(33)H_(44)O_(17)extracted by the best extraction technology was 72.37%and RSD was 0.83%.Therefore,the optimal extraction production technology was determined as 8 times water,3 times extraction,1 hour boiling each time.
引文
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[3]吴培培.张仲景应用牡蜗的规律研究[D].北京中医药大学,2010.Wu P P. Zhang Zhongjing's study of the law of applying worm[D]. Beijing University of Traditional Chinese Medicine,2010.
[4]王伟,陈阿丽.正交试验法优选中药祛痘面霜中药材的提取工艺研究[J].海峡医药,2017,29(4):21-24.Wang W,Wang C L. Study on the Extraction Technology of Chinese Herbal Medicine Extraction from Chinese Herbal Medicine Exfoliation Cream by Orthogonal Test[J]. Channel Medicine,2017,29(4):21-24.
[5]陈坚平,王思鸿.正交试验法优选复方当归苦参片中药材的提取工艺研究[J].中南药学,2008,6(5):543-546.Chen J P,Wang S H. Orthogonal test method to optimize the extraction process of Chinese herbal medicines from Compound Angelica ginseng tablets[J]. South China Pharmacy,2008,6(5):543-546.
[6]马碧峰,李林,许加娟,等.高效液相色谱法测定合欢磁牡口服液含量[J].中兽医医药杂志,2018,37(5):68-70.Ma B F,Li L,Xu J J,et al. High performance liquid chromatography determination of Hehuan Magnet Oral Liquid[J]. Journal of Veterinary Medicine,2018,37(5):68-70.
[7]钟开位,张潮林.正交试验法优选复方参术健脾口服液中药材的提取工艺研究[J].现代医药卫生,2010,26(15):2273-2275.Zhong K Q,Zhang C L. Orthogonal test method to optimize the extraction process of Chinese herbal medicine from compound Shenshuijian spleen oral liquid[J]. Modern Medicine and Health,2010,26(15):2273-2275.
[8]李卿,周兴,欧燕,等.正交试验优选麻芎平喘颗粒中药材的提取工艺[J].中药工艺与制剂,2014,25(15):1377-1379.Li Q,Zhou X,Ou Y,et al. Orthogonal test to optimize the extraction process of Chinese herbal medicines of Maxiongpingchuan granules[J]. Chinese Medicine Process and Preparation,2014,25(15):1377-1379.
[9]钱凯,干国平,方志兴,等.合欢皮水溶性木脂素类化合物的纯化工艺研究[J].时珍国医国药,2015,26(04):790-792.Qian K,Gan G P,Fang Z X,et al. Purification of water-soluble lignin compounds in Huehuan skin[J]. Shi Zhenguo Medicine,2015,26(04):790-792.
[10] Kinjo J,Higuchi H,Fukui K,et al. Studies on leguminous plants. Part XXVI. Lignoids from Albizziae Cortex. II. A Biodegradation Pathway of Syringaresinol[J]. Chemical&Pharmaceutical Bulletin,1991,39(11):2952-2955.
[11]夏洁,舒畅,王庆,等.合欢皮多糖提取工艺的优化及抗氧化研究[J].武汉轻工大学学报,2018,37(5):89-94.Xia J,Shu C,Wang Q,et al. Optimization and Antioxidant Study on Extraction Technology of Acacia Leather Polysaccharide[J]. Journal of the Wuhan Polytechnic University,2018,37(5):89-94.
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