姜枣祛寒颗粒质量标准研究
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摘要
目的建立姜枣祛寒颗粒的质量标准。方法采用薄层色谱(TLC)法对姜枣祛寒颗粒中的干姜、大枣进行定性鉴别;采用高效液相色谱(HPLC)法测定干姜中6-姜辣素的含量,色谱柱采用Accurasil C18柱(250 mm×4. 6 mm,5μm),流动相为乙腈-甲醇-0. 1%磷酸溶液(40∶5∶55),流速为1. 0 m L/min,检测波长为280 nm,进样量为10μL。结果薄层色谱斑点清晰,阴性对照无干扰。干姜中6-姜辣素进样量在46～578 ng范围内与峰面积积分值线性关系良好(r=1);平均回收率为100. 58%,RSD为1. 78%(n=6)。结论该方法操作简便,专属性高,重复性好,可作为姜枣祛寒颗粒的质量控制方法。
Objective To establish the quality standard for Jiangzao Quhan Granules. Methods TLC method was adopted to qualitatively identify Zingberis Rhizoma and Jujubae Fructus in Jiangzao Quhan Granules. The content of 6-gingerol in Zingberis Rhizoma was determined by HPLC,the chromatographic column was Accurasil C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-methanol-0. 1% phosphoric acid solution( 40 ∶ 5 ∶ 55),the flow rate was 1. 0 m L/min,the detection wavelength was 280 nm,and the sample size was 10 μL. Results The TLC spots were clear and the negative control had no interference. The 6-gingerol in Zingberis Rhizoma showed good relationship in the range of 46-578 ng( r = 1). The average recovery rate was 100. 58%,RSD was 1. 78%( n = 6).Conclusion The method is simple,specific and reproducible,which can be used for the quality control of Jiangzao Quhan Granules.
Objective To establish the quality standard for Jiangzao Quhan Granules. Methods TLC method was adopted to qualitatively identify Zingberis Rhizoma and Jujubae Fructus in Jiangzao Quhan Granules. The content of 6-gingerol in Zingberis Rhizoma was determined by HPLC,the chromatographic column was Accurasil C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-methanol-0. 1% phosphoric acid solution( 40 ∶ 5 ∶ 55),the flow rate was 1. 0 m L/min,the detection wavelength was 280 nm,and the sample size was 10 μL. Results The TLC spots were clear and the negative control had no interference. The 6-gingerol in Zingberis Rhizoma showed good relationship in the range of 46-578 ng( r = 1). The average recovery rate was 100. 58%,RSD was 1. 78%( n = 6).Conclusion The method is simple,specific and reproducible,which can be used for the quality control of Jiangzao Quhan Granules.
引文
[1] WS3-B-0361-90,中华人民共和国卫生部药品标准·中药成方制剂(第二册)[S].
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[10]陈艳,周冬翠,张梅.三种方法提取生姜有效部位群并测定6-姜辣素含量[J].中国药师,2014,17(7):1099-1102.
[11]孟喜成,杨青,王青兰.高效液相色谱法测定十滴水中6-姜辣素的含量[J].中国药物与临床,2012,12(2):188-189.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015.
[3]张采,李佳,张永清.大枣化学成分研究概况[J].中国现代中药,2016,13(11):49.
[4]王蓉珍,赵子青,林勤保,等.大枣功效成分检测的研究进展[J].食品工业科技,2012,33(4):423-426.
[5]赵伟,王凤礼.藿香正气丸的薄层色谱检测[J].黑龙江医药,2003,16(1):18-19.
[6]安,周思丽,尤丽莎,等.神怡颗粒的质量标准研究[J].中成药,2008,30(12):1781-1785.
[7]章春宇,庄程,商量.养血饮口服液的质量标准[J].中国药师,2015,18(3):506-507.
[8]吕武清,龙新华.中成药中的药材薄层色谱鉴别[M].北京:人民卫生出版社,1997:44.
[9]钮翠然,陆娟,宋丽丽,等. RP-HPLC法测定干姜中3种姜酚的含量[J].药物分析杂志,2008,28(12):2008-2010.
[10]陈艳,周冬翠,张梅.三种方法提取生姜有效部位群并测定6-姜辣素含量[J].中国药师,2014,17(7):1099-1102.
[11]孟喜成,杨青,王青兰.高效液相色谱法测定十滴水中6-姜辣素的含量[J].中国药物与临床,2012,12(2):188-189.