4株凡纳滨对虾肠道益生菌分离、鉴定及应用研究
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摘要
为将凡纳滨对虾肠道分离的益生菌用于对虾的养殖,利用乳酸菌培养基分离对虾肠道中的益生菌并对菌株进行初步分类鉴定,利用体外抑菌方法筛选有抗菌活性的菌株,用于考察对虾实验室养殖过程中抗有关致病菌的效果。试验结果显示,自虾肠道分离得到W7、W25、W27、W31菌株,分别属于啤酒酵母、短小芽孢杆菌、枯草芽孢杆菌沙漠亚种和暹罗芽孢杆菌,它们对致病菌副溶血弧菌、金黄色葡萄球菌和沙门氏菌有一定的抗菌活性,其中暹罗芽孢杆菌W31抗菌效果最好。将啤酒酵母W7和暹罗芽孢杆菌W31菌体与饲料混合用于对虾的养殖,能够明显增强凡纳滨对虾抵抗弧菌侵染的能力,提高存活率。
The probiotics were isolated from intestine of Pacific white shrimp Litopenaeus vannamei by lactobacillus culture medium, and identified, and antibacterial activity of the strains were studied in vitro in antipathogenic bacteria in the shrimp culture. The strain W7 was identified as Saccharomyces cerevisiae, W25 as Bacillus pumilus, W27 as B. subtilis ssp. inaquosorum, and W31 as B. siamensis, and they showed some antibacterial activity against pathogenic bacteria Vibrio parahaemolyticus, Staphylococcus aureus and Samonella in Pacific white shrimp, with the best effect in B. siamensis W31. The Pacific white shrimp fed the diet containing cells of S. cerevisiae W7 and B. siamensis W31 had high ability to resist the infection of Vibrio and high survival rate.
The probiotics were isolated from intestine of Pacific white shrimp Litopenaeus vannamei by lactobacillus culture medium, and identified, and antibacterial activity of the strains were studied in vitro in antipathogenic bacteria in the shrimp culture. The strain W7 was identified as Saccharomyces cerevisiae, W25 as Bacillus pumilus, W27 as B. subtilis ssp. inaquosorum, and W31 as B. siamensis, and they showed some antibacterial activity against pathogenic bacteria Vibrio parahaemolyticus, Staphylococcus aureus and Samonella in Pacific white shrimp, with the best effect in B. siamensis W31. The Pacific white shrimp fed the diet containing cells of S. cerevisiae W7 and B. siamensis W31 had high ability to resist the infection of Vibrio and high survival rate.
引文
[1] 孙冬岩,孙鸣,潘宝海.枯草芽孢杆菌对水质的净化作用研究[J].饲料研究,2009,9(3):58-59.
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[3] Selvina J,Huxley A J,Lipton A.Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp[J].Aquaculture,2004,230(2):241-248.
[4] Street D A,Sprague V.A new species of Pleistophora (Microsporidia:Pristiophoridae) parasites in the shrimp Palaemonetes pugio[J].Journal of Invertebrate Pathology,1974,23(2):153-156.
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[7] 沈南南,李纯厚,贾晓平,等.小球藻与芽孢杆菌对对虾养殖水质调控作用的研究[J].海洋水产研究,2008,29(2):48-52.
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[10] 邓吉朋,黄建华,江世贵,等.两种微生态制剂在斑节对虾高密度养殖中的应用效果[J].广东农业科学,2014(14):121-125.
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[12] 王亭芳,金风杰,马士禹,等.DGGE技术对南美白对虾养殖水体中的微生物多样性的研究[J].生物技术通报,2012(10):131-136.
[13] 李玉宏,柴鹏程,胡修贵,等.应用RFLP和DGGE技术分析工厂化养殖凡纳滨对虾肠道微生物群落特征[J].渔业科学进展,2014,35(2):83-89.
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[15] 贾丹丹,黄春敏,刘英.六神曲炮制过程中微生物的分离与鉴定[J].中国现代中药,2016,18(3):33-38.
[16] 孙振丽,宣引明,张皓,等.南美白对虾养殖环境及其肠道细菌的多样性分析[J].中国水产科学,2016,23(3):594-605.
[17] 韩宁惦,李卓佳,曹煜成,等.TCBS培养基筛选的虾池细菌分析[J].广东农业科学,2012(7):141-144.
[18] 赵彩春,陈国明,张家学.短小芽胞杆菌HLK8-1 的分离鉴定及抑菌特性分析[J].微生物学杂志,2016,36(2):33-38.
[19] 龚斌,潘育胜,张艳秋,等.凡纳滨对虾细菌性病原的分离鉴定和耐药性研究[J].微生物学杂志,2014,34(5):70-75.
[20] 赵坤.对虾养殖池塘有益芽孢杆菌的分离筛选及特性初步研究[D].青岛:中国海洋大学,2014.
[21] 张盛静.养殖对虾病原性弧菌拮抗菌的筛选和效果评价[D].上海:上海海洋大学,2015.
[22] 夏磊,赵明军,张洪玉,等.不同比例复合益生菌对凡纳滨对虾生长、免疫及抗氨氮能力的影响[J].中国水产科学,2015,22(6):1299-1307.
[23] 裘钱玲琳.微生物菌剂对对虾养殖水体和肠道细菌群落的影响[D].宁波:宁波大学,2015.
[24] 王春迪,宋晓玲,张晓静,等.养殖水体中添加蜡样芽孢杆菌PC465对凡纳滨对虾抗病力的影响[J].中国水产科学,2016,23(1):146-155.
[25] 刘强强,陈旭,谢家俊,等.饲料或养殖水体中添加地衣芽孢杆菌对凡纳滨对虾生长性能和免疫力的影响[J].动物营养学报,2017,29(8):2808-2816.
[2] Lightner D V.Virus disease of farmed shrimp in the Western Hemisphere(the Americas):a review[J].Journal of Invertebrate Pathology,2011,106(1):110-130.
[3] Selvina J,Huxley A J,Lipton A.Immunomodulatory potential of marine secondary metabolites against bacterial diseases of shrimp[J].Aquaculture,2004,230(2):241-248.
[4] Street D A,Sprague V.A new species of Pleistophora (Microsporidia:Pristiophoridae) parasites in the shrimp Palaemonetes pugio[J].Journal of Invertebrate Pathology,1974,23(2):153-156.
[5] Gatesoupe F G,Lesel R.An environmental approach to intestinal microflora in fish[J].Cah Agric,1998,7(1):29-35.
[6] 宛力,王吉桥,高峰,等.南美白对虾肠道细菌菌群分析[J].水产科学,2006,25(1):13-15.
[7] 沈南南,李纯厚,贾晓平,等.小球藻与芽孢杆菌对对虾养殖水质调控作用的研究[J].海洋水产研究,2008,29(2):48-52.
[8] 张云宁,丽军谢,晓晖张,等.芽孢杆菌在沼虾养殖水体中益水作用的研究[J].饲料工业,2010,31(10):27-30.
[9] 孟霄鹏,孟阳,王悦,等.益生菌对凡纳滨对虾免疫功能及肠道菌群的影响[J].水产科学,2017,36(1):60-65.
[10] 邓吉朋,黄建华,江世贵,等.两种微生态制剂在斑节对虾高密度养殖中的应用效果[J].广东农业科学,2014(14):121-125.
[11] 马良骁,冯宪斌,韦新兰,等.水产养殖中微生物制剂的应用及研究进展[J].渔业致富指南,2013(14):12-16.
[12] 王亭芳,金风杰,马士禹,等.DGGE技术对南美白对虾养殖水体中的微生物多样性的研究[J].生物技术通报,2012(10):131-136.
[13] 李玉宏,柴鹏程,胡修贵,等.应用RFLP和DGGE技术分析工厂化养殖凡纳滨对虾肠道微生物群落特征[J].渔业科学进展,2014,35(2):83-89.
[14] 李步先.虾蟹养殖池塘益生菌的分离筛选及降解特性初步研究[D].青岛:中国海洋大学,2015.
[15] 贾丹丹,黄春敏,刘英.六神曲炮制过程中微生物的分离与鉴定[J].中国现代中药,2016,18(3):33-38.
[16] 孙振丽,宣引明,张皓,等.南美白对虾养殖环境及其肠道细菌的多样性分析[J].中国水产科学,2016,23(3):594-605.
[17] 韩宁惦,李卓佳,曹煜成,等.TCBS培养基筛选的虾池细菌分析[J].广东农业科学,2012(7):141-144.
[18] 赵彩春,陈国明,张家学.短小芽胞杆菌HLK8-1 的分离鉴定及抑菌特性分析[J].微生物学杂志,2016,36(2):33-38.
[19] 龚斌,潘育胜,张艳秋,等.凡纳滨对虾细菌性病原的分离鉴定和耐药性研究[J].微生物学杂志,2014,34(5):70-75.
[20] 赵坤.对虾养殖池塘有益芽孢杆菌的分离筛选及特性初步研究[D].青岛:中国海洋大学,2014.
[21] 张盛静.养殖对虾病原性弧菌拮抗菌的筛选和效果评价[D].上海:上海海洋大学,2015.
[22] 夏磊,赵明军,张洪玉,等.不同比例复合益生菌对凡纳滨对虾生长、免疫及抗氨氮能力的影响[J].中国水产科学,2015,22(6):1299-1307.
[23] 裘钱玲琳.微生物菌剂对对虾养殖水体和肠道细菌群落的影响[D].宁波:宁波大学,2015.
[24] 王春迪,宋晓玲,张晓静,等.养殖水体中添加蜡样芽孢杆菌PC465对凡纳滨对虾抗病力的影响[J].中国水产科学,2016,23(1):146-155.
[25] 刘强强,陈旭,谢家俊,等.饲料或养殖水体中添加地衣芽孢杆菌对凡纳滨对虾生长性能和免疫力的影响[J].动物营养学报,2017,29(8):2808-2816.