羊口疮自然病例的分子学诊断及病理形态学观察
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摘要
重庆市某羊场发生了急性热性传染病,通过对发病羊只进行采样并对其进行分子生物学诊断,病理组织H.E.染色观察;通过PCR扩增检测出羊痘特异基因P32为阴性,羊口疮特异性基因B2L为阳性,经测序基因比对确诊为羊口疮;病变组织观察发现在肝、肺、舌组织中炎性细胞浸润,并在组织间隙中发现大量嗜酸性病毒颗粒,肝脏、舌组织部分细胞破裂,肺脏出现增生现象。
A serious orf transmitted in a goat factory in Chongqing,and the epidemic was controlled by emergency immunization and treatment.The orf infection was determined by PCR and sequencing test between the capripox virus specific gene P32 and the orf virus gene B2 L after isolation.Inflammatory cell infiltration and large of eosinophilic bodies were found in the liver,lung and tongue tissues by HE staining of the epidemic samples.
A serious orf transmitted in a goat factory in Chongqing,and the epidemic was controlled by emergency immunization and treatment.The orf infection was determined by PCR and sequencing test between the capripox virus specific gene P32 and the orf virus gene B2 L after isolation.Inflammatory cell infiltration and large of eosinophilic bodies were found in the liver,lung and tongue tissues by HE staining of the epidemic samples.
引文
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[11]苏伟.人工感染绵羊痘病毒甘肃流行株在绵羊体内的分布及病理学研究[D].成都:四川农业大学,2013.
[12]阴正兴,许乐仁,周碧君,等.山羊痘的病理形态学观察[J].中国预防兽医学报,2005,29(5):402-407.
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[2]颜新敏,储岳峰,吴国华,等.山羊痘、羊口疮及山羊传染性胸膜肺炎混合感染的检测与分析[J].中国兽医学报,2011,38(6):809-813.
[3]朱伟,熊毅,刘棋,等.快速鉴别诊断山羊痘病毒和羊口疮病毒二联PCR方法的建立[J].中国预防兽医学报,2007,31(5):394-396.
[4]Chu Y,Yan X,Gao P,et al.Molecular detection of a mixed infection of Goatpox virus,Orf virus,and Mycoplasma capricolum subsp.capripneumoniae in goats[J].J Vet Diagn Invest,2011,23(4):786-789.
[5]Hosamani M,Bhanuprakash V,Scagliarini A,et al.Comparative sequence analysis of major envelope protein gene(B2L)of Indian orf viruses isolated from sheep and goats[J].Vet Microbiol,2006,116(4):317-324.
[6]Fleming S B,Wise L M,Mercer A A.Molecular genetic analysis of orf virus:a poxvirus that has adapted to skin[J].Viruses,2015,7:1505-1539.
[7]Hosamani M,Scagliarini A,Bhanuprakash V,et al.Orf:an update on current research and future perspectives[J].Expert Rev Anti Infect Ther,2009,7(7):879-893.
[8]Haig D M,Mercer A A.Ovine diseases Orf[J].Vet Res,1998,29(3-4):311-326.
[9]Barraviera S.Diseases caused by poxvirus-Orf and milker's nodules-A review[J].Journal of Venomous Animals and Toxins Including Tropical Diseases,2005,11(2):102-108.
[10]尚辰.陕西关中某羊场羊口疮病毒的分离鉴定与基因序列分析[D].杨凌:西北农林科技大学,2014.
[11]苏伟.人工感染绵羊痘病毒甘肃流行株在绵羊体内的分布及病理学研究[D].成都:四川农业大学,2013.
[12]阴正兴,许乐仁,周碧君,等.山羊痘的病理形态学观察[J].中国预防兽医学报,2005,29(5):402-407.
[13]程国富,李红文,周诗其,等.山羊痘自然病例的病理形态学观察及诊断[J].华中农业大学学报,2004,05:543-546.