家用aPTT检测技术芯片修饰方法的研究
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摘要
采用凝血检测光学透射比浊方法检测活化部分凝血活酶时间(aPTT),尝试用聚乙烯(PE)、聚苯乙烯(PS)和Parylene-C 3种疏水材料,研究压电石英晶体微电平(QCM)传感芯片表面修饰。修饰后,芯片品质用网络分析仪衡量,PE、PS和Parylene-C3种疏水材料的疏水修饰接触角分别为92.3°、90.7°和93.3°。结果表明,Parylene-C修饰后QCM芯片的品质提高,有效信号峰-峰值提高了11%;用于光学aPTT检测对比体现出良好重复性,血浆7次检测变异系数(CV)为1.51%,重复性良好,全血7次检测CV为1.15%。本文的Parylene-C修饰QCM,可为家用aPTT检测仪器的开发提供参考。
The optical transmission turbidimetry is used to detect activated partial thromboplastin time(aPTT),and the surface modification of quartz crystal microbalance(QCM)is studied by using three kinds of materials,which include polyethylene(PE),polystyrene(PS)and Parylene-C.Three kinds of modified QCM(PE-QCM,PS-QCM and P-QCM)were evaluated by network analyzer.In addition,hydrophobic contact angles are 92.3°,90.7°and 93.3°,respectively.The results show that the quality of PQCM device is improved,the effective signal peak to peak value is increased by 11%,the comparison of optical aPTT detection shows good repeatability,plasma 7 times detection coefficient of variation(CV)is1.51%,and 7 whole blood tests CVis 1.15%.In a conclusion,Parylene-C modified QCM provides key technology of instrument on domestic aPTT testing.
The optical transmission turbidimetry is used to detect activated partial thromboplastin time(aPTT),and the surface modification of quartz crystal microbalance(QCM)is studied by using three kinds of materials,which include polyethylene(PE),polystyrene(PS)and Parylene-C.Three kinds of modified QCM(PE-QCM,PS-QCM and P-QCM)were evaluated by network analyzer.In addition,hydrophobic contact angles are 92.3°,90.7°and 93.3°,respectively.The results show that the quality of PQCM device is improved,the effective signal peak to peak value is increased by 11%,the comparison of optical aPTT detection shows good repeatability,plasma 7 times detection coefficient of variation(CV)is1.51%,and 7 whole blood tests CVis 1.15%.In a conclusion,Parylene-C modified QCM provides key technology of instrument on domestic aPTT testing.
引文
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[19]ZHANG Jing-yu.Diagnostic thinking of hemorrhagic diseases[J].Clinical review,2015,30(10):1092-1099.张敬宇.出血性疾病的诊断思维[J].临床荟萃,2015,30(10):1092-1099.
[20]SHAO Ming-qin.The effect of comprehensive nursing intervention on patients with dizziness caused by cerebral insufficiency[J].Chinese Journal of continuing medical education,2017,9(1):222.邵明芹.对脑供血不足型头晕患者实施全面护理干预的效果[J].中国继续医学教育,2017,9(1):222.
[21]DENG Jia-dong,YANG Chong-li,YANG Tian-ying.Clinical hematology[M].Shanghai:Shanghai science and Technology Press,2001,7:1380.邓家栋,杨崇礼,杨天楹.临床血液学[M].上海:上海科学技术出版社,2001,77:1380.
[22]LIAO Ri-wei,ZENG Ya-guang,HAN Ding-an,et al.The low frame rate of optical micro angiography[J].Journal of Optoelectronics·Laser,2016,27(4):455-460.廖日威,曾亚光,韩定安,等.全场低频率光学微血管造影成像[J].光电子·激光,2016,27(4):455-460.
[23]GUO Hui-xian,ZHU Qi,LI Yuan-peng,et al.Visible and near infrared spectrum modeling of serum bilirubin content in human serum based on iPLS and SiPLS algorithm[J].Journal of Optoelectronics·Laser,2016,27(10):1136-1144.郭慧娴,朱思祁,黎远鹏,等.基于iPLS和SiPLS算法的人体血清胆红素含量的可见近红外光谱建模[J].光电子·激光,2016,27(10):1136-114
[2]Hussain M.Prothrombin time(PT)for human plasma on QCM-D platform:A better alternative to′gold standard[J].UK J.Pharm.Biosci,2015,3(6):01-08.
[3]Luppa P B.Point-of-care testing(POCT):Current techniques and future perspectives[J].TrAC Trends in Analytical Chemistry,2011,30(6):887-898.
[4]Harris L F,Castro-López V,Killard A J.Coagulation monitoring devices:Past,present,and future at the point of care[J].TrAC Trends in Analytical Chemistry,2013,50:85-95.
[5]CHEN Rong.Tissue optical parameters of Chinese blood[J].Journal of Optielectronics·Laser,2002,13(1):92-93.陈荣.中国人血液的组织光学参数[J].光电子·激光,2002,13(1):92-93.
[6]CHEN Ling.STA COMPACT automatic coagulation analyzer common fault analysis and routine maintenance[J].Thrombosis and hemostasis,2009,15(6):274-275.陈玲.STA COMPACT全自动凝血分析仪常见故障分析及日常保养[J].血栓与止血学,2009,15(6):274-275.
[7]CHEN Ling-Xin.development of piezoelectric crystal sensor[J].Chemical progress,2002,14(1):68-76.陈令新.压电晶体传感器的研究进展[J].化学进展,2002,14(1):68-76.
[8]Hussain M.QCM-D for haemostasis assays:current status and future:a review[J].UK Journal of Pharmaceutical and Biosciences,2016,4(1):121-132.
[9]SHI Xu-Bo,HU Da-Yi.Thrombosis and coagulation mechanism and regulation[J].Clinical review,2007,22(14):989-991.史旭波,胡大一.血栓形成与凝血机制及调节[J].临床荟萃,2007,22(14):989-991.
[10]Van Oss C.Surface properties of fibrinogen and fibrin[J].Journal of protein chemistry,1990,9(4):487-491.
[11]SUN Na-na.The development of prothrombin time detection system based on QCM sensor[A].孙娜娜.基于QCM传感器的凝血酶原时间检测系统的研制[A].
[12]NIU Lei.synthesis and structure analysis of Parylene C monomer[J].Materials review,2010,24(6B):40-42.牛磊.Parylene C单体的合成及其结构解析[J].材料导报,2010,24(6B):40-42.
[13]WANG Yong-gang.Parylene-a new type of protective coating material for airborne equipment[J].Aviation precision manufacturing technology,2002,(1):16-18.王永刚.Parylene-一种新型机载设备覆型防护涂层材料[J].航空精密制造技术,2002,(1):16-18.
[14]CHEN Xi,WU Nian-zeng.C type parylene dog artery implantation:preliminary study on blood compatibility[J].Chinese Journal of Biomedical Engineering,2000,19(2):206-212.陈曦,吴念曾.C型聚对二甲苯狗动脉植入:血液相容性初步研究[J].中国生物医学工程学报,2000,19(2):206-212.
[15]YANG Bei.Synthesis,characterization and properties of biomedical polystyrene adsorption materials[A].杨蓓.生物医用聚苯乙烯吸附材料的合成,表征及性能研究[A].
[16]Andersson M.Quartz crystal microbalance-with dissipation monitoring(QCM-D)for real time measurements of blood coagulation density and immune complement activation on artificial surfaces[J].Biosensors and Bioelectronics,2005,21(1):79-86.
[17]CHEN Rong.The transmission characteristics of light in blood by laser irradiation blood therapy[J].Journal of Optielectronics·Laser,2001,12(12):1310-1312.陈荣.激光照射血液疗法中光在血液中的传输特性[J].光电子·激光,2001,12(12):1310-1312.
[18]Atashbar M Z.QCM biosensor with ultra thin polymer film[J].Sensors and Actuators B:Chemical,2005,107(2):945-951.
[19]ZHANG Jing-yu.Diagnostic thinking of hemorrhagic diseases[J].Clinical review,2015,30(10):1092-1099.张敬宇.出血性疾病的诊断思维[J].临床荟萃,2015,30(10):1092-1099.
[20]SHAO Ming-qin.The effect of comprehensive nursing intervention on patients with dizziness caused by cerebral insufficiency[J].Chinese Journal of continuing medical education,2017,9(1):222.邵明芹.对脑供血不足型头晕患者实施全面护理干预的效果[J].中国继续医学教育,2017,9(1):222.
[21]DENG Jia-dong,YANG Chong-li,YANG Tian-ying.Clinical hematology[M].Shanghai:Shanghai science and Technology Press,2001,7:1380.邓家栋,杨崇礼,杨天楹.临床血液学[M].上海:上海科学技术出版社,2001,77:1380.
[22]LIAO Ri-wei,ZENG Ya-guang,HAN Ding-an,et al.The low frame rate of optical micro angiography[J].Journal of Optoelectronics·Laser,2016,27(4):455-460.廖日威,曾亚光,韩定安,等.全场低频率光学微血管造影成像[J].光电子·激光,2016,27(4):455-460.
[23]GUO Hui-xian,ZHU Qi,LI Yuan-peng,et al.Visible and near infrared spectrum modeling of serum bilirubin content in human serum based on iPLS and SiPLS algorithm[J].Journal of Optoelectronics·Laser,2016,27(10):1136-1144.郭慧娴,朱思祁,黎远鹏,等.基于iPLS和SiPLS算法的人体血清胆红素含量的可见近红外光谱建模[J].光电子·激光,2016,27(10):1136-114