龙岩鲜烟叶特征香气成分前体物代谢组学的通路分析
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摘要
为了探讨龙岩鲜烟叶特征香气成分代谢前体物生物合成过程中的代谢组学通路,采用主成分分析和逐步回归分析双重筛选的方法,筛选龙岩鲜烟叶特征代谢谱;根据KEGG分析信号通路、分子注释、相关酶和生化反应等相关性质,利用Metabo Analyst网络软件可视化代谢产物路径。结果表明,5个代谢产物参与了11条代谢路径,其中类胡萝卜素生物合成和氨酰基t RNA生物合成通路的影响值(P<0.05)。因此,龙岩特征香气成分前体物的生物合成过程与类胡萝卜素生物合成和氨酰基t RNA生物合成通路密切相关。
To clarity the metabonomics pathway of characteristic aroma component precursors in Longyan fresh tobacco leaves, principal component analysis and stepwise regression analysis were used to screen characteristic metabolic profiles and KEGG analysis was used to study the pathway, molecular metabolite annotation, enzyme, biochemical reaction and related properties. Metabo Analyst network software was used to visualize metabolite path.The results showed that 5 metabolites were involved in 11 metabolic pathways in which carotenoid biosynthesis and aminoacyl t RNA biosynthesis presented significant influence(P<0.05). Therefore, biosynthesis process of characteristic aroma component precursors is closely related with the two pathways.
To clarity the metabonomics pathway of characteristic aroma component precursors in Longyan fresh tobacco leaves, principal component analysis and stepwise regression analysis were used to screen characteristic metabolic profiles and KEGG analysis was used to study the pathway, molecular metabolite annotation, enzyme, biochemical reaction and related properties. Metabo Analyst network software was used to visualize metabolite path.The results showed that 5 metabolites were involved in 11 metabolic pathways in which carotenoid biosynthesis and aminoacyl t RNA biosynthesis presented significant influence(P<0.05). Therefore, biosynthesis process of characteristic aroma component precursors is closely related with the two pathways.
引文
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[3]周芳芳,张晓龙,詹军,等.典型清香型烤烟产地间致香物质含量及组成差异分析[J].中国烟草学报,2016,22(2):34-42.
[4]耿召良,李继新,冯永刚,等.生态条件和种质因素对初烤烟叶次生代谢产物含量的影响[J].西北农林科技大学学报(自然科学版),2013,41(12):80-85.
[5]张志高,李立新,饶文平,等.不同移栽期对烤烟K326化学成分及中性香气成分的影响[J].江西农业大学学报,2015,37(3):423-428.
[6]吕婧,刘贯山,晁江涛,等.影响烟草香气物质合成代谢途径关键酶基因的研究进展[J].中国农学通报,2014,30(4):49-57.
[7]赵会娜,蔡凯,雷波,等.烤烟中性致香物质在烘烤前后的差异分析[J].中国烟草科学,2015,36(2):8-13.
[8]谢贤斌,多变数超饱和模型回归分析的重复筛选新方法[D].扬州:扬州大学,2015.
[9]ZHAO Y N,ZHAO J Y,ZHAO C X,et al.A metabolomics study delineating geographical location-associated primary metabolic changes in the leaves of growing tobacco plants by GC-MS and CE-MS[J].Sci Rep-UK,2015(5):16346.
[10]邹权,陈启安,曾湘祥,等.系统生物学中的网络分析方法[M].西安:西安电子科技大学出版社,2015.
[11]XIA J,SINELNIKOV I V,HAN B,et al.Metabo Analyst3.0:making metabolomics more meaningful[J].Nucleic Acids Research,2015,43(W1):W251-W257.
[12]杨红旗.中国烤烟主要香气前体物的研究[D].长沙:湖南农业大学,2005.
[13]腊贵晓,郝西,理向阳,等.代谢组学在植物研究领域中的应用[J].植物学研究,2016,5(1):26-33.
[14]HAVRYLENKO S,MIRANDE M.Aminoacyl-t RNA synthetase complexes in evolution[J].Int J Mol Sci,2015,16(3):6571-6594.
[15]王镜岩,朱圣庚,徐长法.生物化学[M].3版.北京:高等教育出版社,2002.