青檀二倍体及人工诱导的同源四倍体遗传差异的AFLP分析
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摘要
为了从DNA分子水平探讨二倍体青檀(2n=2x=18)与经秋水仙素诱变获得的同源四倍体青檀(2n=4x=36)在遗传结构上的差异,本研究以2x及同源4x青檀为实验材料,利用AFLP(Amplified Fragment Length Polymorphism)分子标记技术,对供试材料的DNA多态性及遗传距离进行分析。结果表明:经秋水仙素诱变获得的同源4x青檀与2x青檀在DNA分子遗传结构上具有显著差异,2x青檀单株间DNA多态性大于同源4x青檀,4x青檀单株间遗传距离在总体水平上大于2x青檀。本研究结果为进一步开展青檀多倍体育种、发挥青檀多倍体的优势提供理论依据。
The paper aims to study the genetic diversity between diploid and homologous tetraploid of Pteroceltis tatarinowii Maxim chemically induced by colchicines from the DNA molecule level. We took thediploid and homologous tetraploid of Pteroceltis tatarinowii Maxim. as experimental materials and analyzed theDNA polymorphism and genetic distance by the AFLP molecular markers. The results showed that: there weresignificant differences in the DNA molecular genetic structure between homologous tetraploid induced bycolchicines and diploid of Pteroceltis tatarinowii Maxim., and the DNA polymorphism of the diploid was higherthan that of homologous tetraploid and the genetic distance between individual plants of tetraploid was higherthan that of diploid in the whole level. The result provides a basis for further development of polyploid breedingand taking advantages of polyploid of Pteroceltis tatarinowii Maxim.
The paper aims to study the genetic diversity between diploid and homologous tetraploid of Pteroceltis tatarinowii Maxim chemically induced by colchicines from the DNA molecule level. We took thediploid and homologous tetraploid of Pteroceltis tatarinowii Maxim. as experimental materials and analyzed theDNA polymorphism and genetic distance by the AFLP molecular markers. The results showed that: there weresignificant differences in the DNA molecular genetic structure between homologous tetraploid induced bycolchicines and diploid of Pteroceltis tatarinowii Maxim., and the DNA polymorphism of the diploid was higherthan that of homologous tetraploid and the genetic distance between individual plants of tetraploid was higherthan that of diploid in the whole level. The result provides a basis for further development of polyploid breedingand taking advantages of polyploid of Pteroceltis tatarinowii Maxim.
引文
[1]张天麟.园林树木1600种[M].北京:中国建筑工业出版社,2011:115.
[2]陈有民.园林树木学[M].北京:中国林业出版社,1990:328.
[3]刘福妹,穆怀志,刘子嘉,等.用秋水仙素处理不同家系白桦种子诱导四倍体的研究[J].北京林业大学学报,2013,35(3):84-89.
[4]康向阳.林木多倍体育种研究进展[J]北京林业大学学报,2003,25(4):70-73.
[5]高鹏,林威,康向阳.秋水仙素诱导杜仲花粉染色体加倍的研究[J]北京林业大学学报,2004,26(4):39-42.
[6] Einsphahr D W. Production and utilization of triploid hybridAspen[J].Iowa State Journal of Research,1984,58(4):401-409.
[7]张莉,张小平,路畅.安徽琅琊山青檀种群空间格局[J].林业科学,2012,48(2):9-11.
[8]侯嫦英,方升佐,薛建辉,等.干旱胁迫对青檀等树种苗木生长及生理特性的影响[J]南京林业大学学报报,2003,27(6):103-106.
[9]洑香香,方升佐,杜艳.青檀种子休眠机理及发芽条件的探讨[J].植物资源与环境学报,2002,11(1):9-13.
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[13]方升佐,李光友.经营措施对青檀人工林生物量及檀皮质量的影响[J].南京林业大学报,2001,25(1):21-24.
[14]刘慧君,张莉,王芳,等.11个种群青檀檀皮差异及其与环境因子的关系[J].南京林业大学学报:自然科学版,2015,39(3):78-81.
[15]李晓红,张慧,王德元,等.我国特有植物青檀遗传结构的ISSR分析[J].安徽农业科学,2013,33(16):4892-4895.
[16]杨继.植物多倍体基因组的形成与进化[J].植物分类学报,2001,39(4):357-371.
[17]张志胜,黎扬辉,姜蕾,等.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007,34(3):729-734.
[18]张海凤,郭宝林,张成合,等.杜仲四倍体的诱导与鉴定[J].园艺学报,2008,35(7):1047-1052.
[19]张丽丽,张蜀宁,张红梅,等.同源四倍体青花菜离体再生及其倍性鉴定[J].园艺学报,2008,35(10):1517-1520.
[20]张蜀宁,张丽丽,唐君,等.秋水仙素离体诱导同源四倍体青花菜[J].园艺学报,2009,36(11):1681-1684.
[21] Lin Zhang, Feng Wang, Zhongkui Sun, et al. Polyploidy Induction of Pteroceltis tatarinowii Maxim.[J].Agricultural Science&Technology,2015,16(6):1159-1161.
[22]焦锋,楼程富,张有做,等.桑树变异株系基因组DNA扩增多态性RAPD研究[J].蚕业科学,2001,27(3):165-169.
[23]张有做,楼程富,周金妹,等.不同倍性桑品种基因组DNA多态性比较[J].浙江农业大学学报,19988,24(1):79-81.
[24]王卓伟,余茂德,鲁成.桑树二倍体及人工诱导的同源四倍体遗传差异的AFLP分析[J].植物学通报,2002,19(2):194-200.
[25]陈锦华,周力,高和平,等.秋水仙素诱导辣椒染色体结构变异初探[J].湖北农业科学,1995(6):53-55.
[26]马国斌.西瓜甜瓜体细胞无性系的发生、变异与遗传转化研究[D].杨凌:西北农业大学,1997:5-7.
[27] Song K M, Osbom T C. A method for examining expression of homologous genes in plant polyploid[J].Plant Molecular,1994,26:1065-1071.
[28] Guo M, Davis D, Birchler J A. Dosage effects on gene expression in a maize ploidy series[J].Genetics,1996,142(4):1349-1355.
[29] Lee H S, Chen Z J. Protein-coding genes are epigenetically regulated in Arabidopsis polyploids[J].Pro Natl Acad Sci USA.2001,98:6753-6758.
[2]陈有民.园林树木学[M].北京:中国林业出版社,1990:328.
[3]刘福妹,穆怀志,刘子嘉,等.用秋水仙素处理不同家系白桦种子诱导四倍体的研究[J].北京林业大学学报,2013,35(3):84-89.
[4]康向阳.林木多倍体育种研究进展[J]北京林业大学学报,2003,25(4):70-73.
[5]高鹏,林威,康向阳.秋水仙素诱导杜仲花粉染色体加倍的研究[J]北京林业大学学报,2004,26(4):39-42.
[6] Einsphahr D W. Production and utilization of triploid hybridAspen[J].Iowa State Journal of Research,1984,58(4):401-409.
[7]张莉,张小平,路畅.安徽琅琊山青檀种群空间格局[J].林业科学,2012,48(2):9-11.
[8]侯嫦英,方升佐,薛建辉,等.干旱胁迫对青檀等树种苗木生长及生理特性的影响[J]南京林业大学学报报,2003,27(6):103-106.
[9]洑香香,方升佐,杜艳.青檀种子休眠机理及发芽条件的探讨[J].植物资源与环境学报,2002,11(1):9-13.
[10]杨磊,马春萍,姚红宇.青檀在豫北地区的引种推广[J].现代园艺,2013(5):122-123.
[11]王芬.枣庄地区青檀生物学观察及种子育苗试验[J].中国园艺文摘,2012(6):15-17.
[12]段凤芝.优质青檀苗培育技术及密度效应探讨[J].安徽农业科学,2013,41(22):9363-9364.
[13]方升佐,李光友.经营措施对青檀人工林生物量及檀皮质量的影响[J].南京林业大学报,2001,25(1):21-24.
[14]刘慧君,张莉,王芳,等.11个种群青檀檀皮差异及其与环境因子的关系[J].南京林业大学学报:自然科学版,2015,39(3):78-81.
[15]李晓红,张慧,王德元,等.我国特有植物青檀遗传结构的ISSR分析[J].安徽农业科学,2013,33(16):4892-4895.
[16]杨继.植物多倍体基因组的形成与进化[J].植物分类学报,2001,39(4):357-371.
[17]张志胜,黎扬辉,姜蕾,等.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007,34(3):729-734.
[18]张海凤,郭宝林,张成合,等.杜仲四倍体的诱导与鉴定[J].园艺学报,2008,35(7):1047-1052.
[19]张丽丽,张蜀宁,张红梅,等.同源四倍体青花菜离体再生及其倍性鉴定[J].园艺学报,2008,35(10):1517-1520.
[20]张蜀宁,张丽丽,唐君,等.秋水仙素离体诱导同源四倍体青花菜[J].园艺学报,2009,36(11):1681-1684.
[21] Lin Zhang, Feng Wang, Zhongkui Sun, et al. Polyploidy Induction of Pteroceltis tatarinowii Maxim.[J].Agricultural Science&Technology,2015,16(6):1159-1161.
[22]焦锋,楼程富,张有做,等.桑树变异株系基因组DNA扩增多态性RAPD研究[J].蚕业科学,2001,27(3):165-169.
[23]张有做,楼程富,周金妹,等.不同倍性桑品种基因组DNA多态性比较[J].浙江农业大学学报,19988,24(1):79-81.
[24]王卓伟,余茂德,鲁成.桑树二倍体及人工诱导的同源四倍体遗传差异的AFLP分析[J].植物学通报,2002,19(2):194-200.
[25]陈锦华,周力,高和平,等.秋水仙素诱导辣椒染色体结构变异初探[J].湖北农业科学,1995(6):53-55.
[26]马国斌.西瓜甜瓜体细胞无性系的发生、变异与遗传转化研究[D].杨凌:西北农业大学,1997:5-7.
[27] Song K M, Osbom T C. A method for examining expression of homologous genes in plant polyploid[J].Plant Molecular,1994,26:1065-1071.
[28] Guo M, Davis D, Birchler J A. Dosage effects on gene expression in a maize ploidy series[J].Genetics,1996,142(4):1349-1355.
[29] Lee H S, Chen Z J. Protein-coding genes are epigenetically regulated in Arabidopsis polyploids[J].Pro Natl Acad Sci USA.2001,98:6753-6758.