蝌蚪红细胞微核试验在水环境污染状况监测中的应用
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摘要
通过测定蝌蚪血红细胞微核率,对淮安市区及周边不同区域水质诱发蝌蚪红细胞微核及核异常进行比较研究.结果表明:染毒1w后,淮安市里运河(淮海路段与黄河路交叉点)、淮安市开发区古淮河水(大同路和水渡口大道交叉点)、淮安市淮安区文渠组(大八字桥至北水关段)水质诱发蝌蚪红细胞微核率均大于对照组(P<0.01).提示淮安市里运河、开发区古淮河水、淮安区文渠水水质对蝌蚪红细胞具有显著的遗传毒性,水样中有致变物的存在.
This paper mainly investigates and compares the effects of water bodies from Huai'an city and the peripheral regions induced by the existence of the micronucleus and the abnormal nucleus of tadpole red blood cells. The results show that the inducing rates of tadpole red blood corpuscles micronucleus cell are higher than the control( P < 0. 01) after the tadpoles have lived in water for one week. The results suggest that the water really contain some remarkable hereditary toxicity to tadpole's red blood corpuscles,thus it is concluded that there are mutational substances in liyun river,kaifaqu river and wenqu river.
This paper mainly investigates and compares the effects of water bodies from Huai'an city and the peripheral regions induced by the existence of the micronucleus and the abnormal nucleus of tadpole red blood cells. The results show that the inducing rates of tadpole red blood corpuscles micronucleus cell are higher than the control( P < 0. 01) after the tadpoles have lived in water for one week. The results suggest that the water really contain some remarkable hereditary toxicity to tadpole's red blood corpuscles,thus it is concluded that there are mutational substances in liyun river,kaifaqu river and wenqu river.
引文
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[10]耿德贵,等.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响[J].动物学杂志,2000,35(1):12-16.
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[12]苑宇哲,等.应用蝌蚪快速检测环境变异的两种方法——微核试验和单细胞凝胶电泳[J].四川动物,2004,23(1):74-80.
[13]贺维顺,王蕊芳.蝌蚪血红细胞微核和核异常监测水质污染的研究[J].动物学研究,1990,11(1):1-6.
[14]刘清华,等.肝癌高发区饮用水对蝌蚪红细胞微核率影响的研究[J].广西预防医学,1999,4(5):72-74.
[2]Jaylet A,et al.A new micronucleus test using peripheral blood erythrocytes of the new t pleurodeles walt to detect mutagens in fresh-water pollution[J].Mutation Res,1986,164(4):245-257.
[3]刘爱华,等.鱼类血细胞的微核测定[J].动物学研究,1985,6(1):8-10.
[4]楼允东,等.亚硝基胍对泥鳅红细胞微核及核异常的诱发[J].中国环境科学,1986,16(4):275-278.
[5]贺维顺,等.一种水质污染生物监测的新方法-蝌蚪肠细胞染色体畸变分析[J].环境科学学报,1987,7(4):467-470.
[6]汤新慧.串场河水诱发蝌蚪血红细胞微核的研究[J].江苏预防医学,1997,7(1):83-86.
[7]周雪瑞.里运河水对蝌蚪红细胞微核率及核异常率的影响[J].江苏预防医学,2005,16(4):102-105.
[8]周雪瑞.淮安市不同区域河流水质诱发蝌蚪红细胞微核及核异常的研究[J].淮阴师范学院学报,2005,4(4):62-65.
[9]耿德贵,等.除草剂乙草胺对蝌蚪红细胞微核及核异常的影响[J].环境与健康杂志,2000,17(1):36-38
[10]耿德贵,等.四种除草剂对中华大蟾蜍蝌蚪红细胞微核及核异常的影响[J].动物学杂志,2000,35(1):12-16.
[11]李春喜,王志和,等.生物统计学[M].北京:科学出版社,2000.
[12]苑宇哲,等.应用蝌蚪快速检测环境变异的两种方法——微核试验和单细胞凝胶电泳[J].四川动物,2004,23(1):74-80.
[13]贺维顺,王蕊芳.蝌蚪血红细胞微核和核异常监测水质污染的研究[J].动物学研究,1990,11(1):1-6.
[14]刘清华,等.肝癌高发区饮用水对蝌蚪红细胞微核率影响的研究[J].广西预防医学,1999,4(5):72-74.