间质性肺疾病患者呼出气冷凝液及支气管肺泡灌洗液中8-iso-PG、LTB4的对比研究
|
摘要
目的研究间质性肺疾病(ILD)患者呼出气冷凝液(EBC)与支气管肺泡灌洗液(BALF)中炎症因子的相关性,探讨EBC在ILD中的临床价值。方法选择ILD患者(ILD组)20例及同期健康体检人群(对照组)20例,收集ILD患者EBC和BALF,对照组收集EBC,采用ELISA法检测EBC和BALF中8-异前列腺素(8-iso-PG)及白三烯B4(LTB4)水平,并分别将ILD患者EBC与BALF中的8-iso-PG及LTB4水平进行相关性分析。结果 ILD组患者EBC中8-iso-PG及LTB4质量浓度均高于对照组(P<0.05,P<0.01);ILD患者EBC与BALF中8-iso-PG及LTB4水平呈正相关(r=0.72,P<0.01;r=0.69,P<0.001)。结论 ILD患者EBC与BALF中炎症因子水平正相关,EBC或可作为无创方法评估ILD患者气道炎症及氧化应激的程度。
Objective To investigate the relativity of inflammatory cytokines in exhaled breath condensate(EBC)and bronchoalveolar lavage fluid(BALF)in patients with interstitial lung diseases(ILD),and to investigate the clinical value of EBC in ILD.Methods The EBC and BALF were collected from 20 ILD patients and the EBC was collected from 20 healthy controls.The levels of8-iso-prostaglandin(8-iso-PG)and leukotriene B4(LTB4)were measured by ELISA,and the correlation between EBC and BALF was analyzed.Results EBC concentrations of 8-iso-PG and LTB4 in ILD patients were higher than those in healthy controls(P<0.05 and P<0.01,respectively).The 8-iso-PG and LTB4 levels in EBC were positively correlated with those in BALF in ILD patients(r=0.72 and r=0.69,respectively;P<0.01 and P<0.001,respectively).Conclusion The levels of inflammatory cytokines in EBC are positively correlated with those in BALF in ILD patients.The EBC can be used as a noninvasive way for evaluating airway inflammation and oxidative stress in ILD patients.
Objective To investigate the relativity of inflammatory cytokines in exhaled breath condensate(EBC)and bronchoalveolar lavage fluid(BALF)in patients with interstitial lung diseases(ILD),and to investigate the clinical value of EBC in ILD.Methods The EBC and BALF were collected from 20 ILD patients and the EBC was collected from 20 healthy controls.The levels of8-iso-prostaglandin(8-iso-PG)and leukotriene B4(LTB4)were measured by ELISA,and the correlation between EBC and BALF was analyzed.Results EBC concentrations of 8-iso-PG and LTB4 in ILD patients were higher than those in healthy controls(P<0.05 and P<0.01,respectively).The 8-iso-PG and LTB4 levels in EBC were positively correlated with those in BALF in ILD patients(r=0.72 and r=0.69,respectively;P<0.01 and P<0.001,respectively).Conclusion The levels of inflammatory cytokines in EBC are positively correlated with those in BALF in ILD patients.The EBC can be used as a noninvasive way for evaluating airway inflammation and oxidative stress in ILD patients.
引文
[1]杨伟煌,黄平.呼出气冷凝液在COPD、哮喘、肺癌及间质性肺疾病中应用的研究进展[J].中国医学创新,2015,12(10):153-156.
[2]晏斌林,顾为丽,杜娟,等.慢性阻塞性肺疾病患者呼出气冷凝液IL-17、IL-10、8-iso-PG的测定及临床意义[J].中国呼吸与危重监护杂志,2017,16(2):142-146.
[3]任珏辉,陈建荣,黄磊,等.ARDS患者呼出气冷凝液和血清中NO、ET-1和8-iso-PG检测的临床意义[J].海南医学,2016,27(24):4023-4026.
[4]巫翠华,黄立霞,李志莹,等.持续气道正压通气对阻塞性睡眠呼吸暂停低通气综合征呼出气冷凝液内皮素-1水平的影响[J].中国医药导报,2015,12(1):30-34.
[5]朱杰,吕学东,马航,等.支气管哮喘患者呼出气冷凝液中LTB-4和TNF-α检测的研究[J].内科急危重症杂志,2016,22(4):279-281.
[6]American Thoracic Society,European Respiratory Society.American Thoracic Society European Respiratory Society international multidisciplinary consensus classification of the idiopathic interstitial pneumonias[J].Am J RespirCrit Care Med,2002,165(2):277-304.
[7]Travis W D,Costabel U,Hansell D M,et al.An Official American Thoracic Society/European Respiratory Society Statement:Update of the international multidisciplinary classification of the idiopathic interstitial pneumonias[J].Am J Respir Crit Care Med,2013,188:733-748.
[8]中华医学会呼吸疾病学会.结节病诊断及治疗方案(第三次修订稿草案)[J].中华结核和呼吸杂志,1994,17(1):9-10.
[9]中华医学会呼吸病学分会.支气管肺泡灌洗液细胞学检测技术规范(草案)[J].中华结核和呼吸杂志[J],2002,25(7):390-391.
[10]Meyer K C,Raghu G,Baughman R P,et al.An official American Thoracic Society clinical practice guideline:theclinical utility of bronchoalveolar lavage cellular analysisin interstitial lung disease[J].Am J Respir Crit Care Med,2012,185(9):1004-1014.
[11]蔡后荣,李惠萍.实用间质性肺疾病[M].北京:人民卫生出版社,2010:4.
[12]Ugurlu E,Kilic Toprak E,Altinisik G,et al.Increased erythrocyte aggregation and oxidative stress in patients with idiopathic interstitial pneumonia[J].Sarcoidosis Vasc Diffuse Lung Dis,2016,33(4):308-316.
[13]蔡柏蔷,李龙芸.协和呼吸病学[M].北京:中国协和医科大学出版社,2005:1413-1414.
[14]Shimizu Y,Dobashi K,Sano T,et al.ROCK activation in lung of idiopathic pulmonary fibrosis with oxidative stress[J].Int J Immunopathol Pharmacol,2014,27(1):37-44.
[15]候显明,于润江.间质性肺病学[M].北京:人民卫生出版社,2003:3.
[16]Tufvesson E,Bozovic G,Hesselstrand R,et al.Increased cysteinyl-leukotrienes and 8-isoprostane in exhaled breath condensate from systemic sclerosis patients[J].Rheumatology(Oxford),2010,49(12):2322-2326.
[17]中国医师协会呼吸医师分会.无创气道炎症评估支气管哮喘的临床应用中国专家共识[J].中华结核和呼吸杂志,2015,38(5):329-341.
[18]Piotrowski W J,Antczak A,Marczak J,et,al.Eicosanoids in exhaled breath condensate and BAL fluid of patients with sarcoidosis[J].Chest,2007,132(2):589-596.
[19]PelclováD,FenclováZ,Kacer P,et al.8-isoprostane and leukotrienes in exhaled breath condensate in Czech subjects with silicosis[J].Ind Health,2007,45:766-774.
[20]Piotrowski W J,Kurmanowska Z,Antczak A,et al.Exhaled8-isoprostane as a prognostic marker in sarcoidosis.A short term follow-up[J].BMC Pulm Med,2010,10:23.
[21]Kowalska A,Pu s′cińska E,Czerniawska J,et al.Markers of fibrosis and inflammation in exhaled breath condensate(EBC)and bronchoalveolar lavage fluid(BALF)of patients with pulmonary sarcoidosis:apilot study[J].Pneumonol Alergol Pol,2010,78(5):356-362.
[22]Jackson A S,Sandrini A,Campbell C,et al.Comparison of biomarkers in exhaled breath condensate and bronchoalveolar lavage[J].Am J Respir Crit Care Med,2007,175(3):222-227.
[2]晏斌林,顾为丽,杜娟,等.慢性阻塞性肺疾病患者呼出气冷凝液IL-17、IL-10、8-iso-PG的测定及临床意义[J].中国呼吸与危重监护杂志,2017,16(2):142-146.
[3]任珏辉,陈建荣,黄磊,等.ARDS患者呼出气冷凝液和血清中NO、ET-1和8-iso-PG检测的临床意义[J].海南医学,2016,27(24):4023-4026.
[4]巫翠华,黄立霞,李志莹,等.持续气道正压通气对阻塞性睡眠呼吸暂停低通气综合征呼出气冷凝液内皮素-1水平的影响[J].中国医药导报,2015,12(1):30-34.
[5]朱杰,吕学东,马航,等.支气管哮喘患者呼出气冷凝液中LTB-4和TNF-α检测的研究[J].内科急危重症杂志,2016,22(4):279-281.
[6]American Thoracic Society,European Respiratory Society.American Thoracic Society European Respiratory Society international multidisciplinary consensus classification of the idiopathic interstitial pneumonias[J].Am J RespirCrit Care Med,2002,165(2):277-304.
[7]Travis W D,Costabel U,Hansell D M,et al.An Official American Thoracic Society/European Respiratory Society Statement:Update of the international multidisciplinary classification of the idiopathic interstitial pneumonias[J].Am J Respir Crit Care Med,2013,188:733-748.
[8]中华医学会呼吸疾病学会.结节病诊断及治疗方案(第三次修订稿草案)[J].中华结核和呼吸杂志,1994,17(1):9-10.
[9]中华医学会呼吸病学分会.支气管肺泡灌洗液细胞学检测技术规范(草案)[J].中华结核和呼吸杂志[J],2002,25(7):390-391.
[10]Meyer K C,Raghu G,Baughman R P,et al.An official American Thoracic Society clinical practice guideline:theclinical utility of bronchoalveolar lavage cellular analysisin interstitial lung disease[J].Am J Respir Crit Care Med,2012,185(9):1004-1014.
[11]蔡后荣,李惠萍.实用间质性肺疾病[M].北京:人民卫生出版社,2010:4.
[12]Ugurlu E,Kilic Toprak E,Altinisik G,et al.Increased erythrocyte aggregation and oxidative stress in patients with idiopathic interstitial pneumonia[J].Sarcoidosis Vasc Diffuse Lung Dis,2016,33(4):308-316.
[13]蔡柏蔷,李龙芸.协和呼吸病学[M].北京:中国协和医科大学出版社,2005:1413-1414.
[14]Shimizu Y,Dobashi K,Sano T,et al.ROCK activation in lung of idiopathic pulmonary fibrosis with oxidative stress[J].Int J Immunopathol Pharmacol,2014,27(1):37-44.
[15]候显明,于润江.间质性肺病学[M].北京:人民卫生出版社,2003:3.
[16]Tufvesson E,Bozovic G,Hesselstrand R,et al.Increased cysteinyl-leukotrienes and 8-isoprostane in exhaled breath condensate from systemic sclerosis patients[J].Rheumatology(Oxford),2010,49(12):2322-2326.
[17]中国医师协会呼吸医师分会.无创气道炎症评估支气管哮喘的临床应用中国专家共识[J].中华结核和呼吸杂志,2015,38(5):329-341.
[18]Piotrowski W J,Antczak A,Marczak J,et,al.Eicosanoids in exhaled breath condensate and BAL fluid of patients with sarcoidosis[J].Chest,2007,132(2):589-596.
[19]PelclováD,FenclováZ,Kacer P,et al.8-isoprostane and leukotrienes in exhaled breath condensate in Czech subjects with silicosis[J].Ind Health,2007,45:766-774.
[20]Piotrowski W J,Kurmanowska Z,Antczak A,et al.Exhaled8-isoprostane as a prognostic marker in sarcoidosis.A short term follow-up[J].BMC Pulm Med,2010,10:23.
[21]Kowalska A,Pu s′cińska E,Czerniawska J,et al.Markers of fibrosis and inflammation in exhaled breath condensate(EBC)and bronchoalveolar lavage fluid(BALF)of patients with pulmonary sarcoidosis:apilot study[J].Pneumonol Alergol Pol,2010,78(5):356-362.
[22]Jackson A S,Sandrini A,Campbell C,et al.Comparison of biomarkers in exhaled breath condensate and bronchoalveolar lavage[J].Am J Respir Crit Care Med,2007,175(3):222-227.