温度胁迫对点蜂缘蝽成虫呼吸代谢关键酶活性的影响
|
摘要
【目的】为明确温度对点蜂缘蝽Riptortus pedestris成虫呼吸代谢关键酶活性的影响。【方法】本研究利用生化方法测定了黑暗条件下经16,20,24,28,32和36℃处理4 h的点蜂缘蝽成虫呼吸代谢关键酶3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)、3-羟酰辅酶A脱氢酶(HOAD)、柠檬酸合成酶(CS)和乳酸脱氢酶(LDH)的活性。【结果】点蜂缘蝽成虫体内5种呼吸代谢关键酶的活性均随温度升高呈现先增后减的趋势。其中点蜂缘蝽成虫雌、雄个体之间的CS活性在16和28℃时存在显著差异,16℃时雌虫CS活性较高,28℃时雄虫CS活性较高;雄虫LDH活性在36℃时显著高于雌虫,提示雌、雄个体间在低温下体内三羧酸循环代谢存在显著差异,在高温下体内糖无氧酵解水平存在显著差异。不同温度处理下雌、雄成虫的GAPDH与HOAD活性比值均远大于1. 0,说明在试验温度下点蜂缘蝽呼吸代谢消耗以糖类为主。【结论】在16℃~36℃范围内,随着温度的升高点蜂缘蝽雌、雄成虫体内呼吸代谢关键酶活性呈现先增后减的趋势,点蜂缘蝽可通过调节呼吸代谢强度适应温度变化。
【Aim】This study aims to determine the effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of the bean bug,Riptortus pedestris.【Methods】The activities of five key enzymes related to respiratory metabolism,i.e.,glyceraldehyde-3-phosphate dehydrogenase( GAPDH),glycerol-3-phosphate( GDH),3-hydroxyacyl-CoA dehydrogenase( HOAD),citrate synthase( CS),and lactate dehydrogenase( LDH),in R.pedestris adults exposed to different temperatures( 16,20,24,28,32 and 36℃) for 4 h in scotophase were assayed by using biochemical methods.【Results】The results showed that the activities of the five key enzymes in R.pedestris adults increased first and then decreased as the temperature increased.The CS activities at 16 and 28℃ differedsignificantly between males and females,being higher in females than in males at 16℃,but lower in females than in males at 28℃.The LDH activities in males were higher than those in females at 36℃,suggesting that there are significant differences between males and females in the tricarboxylic acid cycle metabolism at low temperature and in the anaerobic glycolysis at high temperature.The ratios of GAPDH activity to HOAD activity in adult males and females exposed to different temperatures were much higher than 1.0,indicating that R.pedestri adults mainly consume carbohydrates in respiratory metabolism at the test temperatures.【Conclusion】In the temperature range from 16℃ to 36℃,with the increase of temperature,the activities of key enzymes involved in respiratory metabolism in female and male adults of R.pedestris increase at first,and then decrease.R.pedestris can adjust its respiratory metabolic intensity to some extent to adapt to temperature change.
【Aim】This study aims to determine the effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of the bean bug,Riptortus pedestris.【Methods】The activities of five key enzymes related to respiratory metabolism,i.e.,glyceraldehyde-3-phosphate dehydrogenase( GAPDH),glycerol-3-phosphate( GDH),3-hydroxyacyl-CoA dehydrogenase( HOAD),citrate synthase( CS),and lactate dehydrogenase( LDH),in R.pedestris adults exposed to different temperatures( 16,20,24,28,32 and 36℃) for 4 h in scotophase were assayed by using biochemical methods.【Results】The results showed that the activities of the five key enzymes in R.pedestris adults increased first and then decreased as the temperature increased.The CS activities at 16 and 28℃ differedsignificantly between males and females,being higher in females than in males at 16℃,but lower in females than in males at 28℃.The LDH activities in males were higher than those in females at 36℃,suggesting that there are significant differences between males and females in the tricarboxylic acid cycle metabolism at low temperature and in the anaerobic glycolysis at high temperature.The ratios of GAPDH activity to HOAD activity in adult males and females exposed to different temperatures were much higher than 1.0,indicating that R.pedestri adults mainly consume carbohydrates in respiratory metabolism at the test temperatures.【Conclusion】In the temperature range from 16℃ to 36℃,with the increase of temperature,the activities of key enzymes involved in respiratory metabolism in female and male adults of R.pedestris increase at first,and then decrease.R.pedestris can adjust its respiratory metabolic intensity to some extent to adapt to temperature change.
引文
Addo-Bediako A,Chown SL,Gaston KJ,2002.Metabolic cold adaptation in insects:a large-scale perspective.Funct.Ecol.,16(3):332-338.
Bale JS,Masters GJ,Hodkinson ID,Awmack C,Bezemer TM,Brown VK,Butterfield J,Buse A,Coulson JC,Farrar J,Good JEG,Harrington R,Hartley S,Jones TH,Lindroth RL,Prss MC,Symrnioudis I,Watt AD,Whittaker J,2002.Herbivory in global climate change research:direct effects of rising temperature on insect herbivores.Glob.Chan.Biol.,8(1):1-16.
Beenakkers AMT,1969.Carbohydrate and fat as a fuel for insect flight:a comparative study.J.Insect Physiol.,15:353-361.
Beenakkers AMT,Van der Horst DJ,Van Marrewijk WJA,1984.Insect flight muscle metabolism.Insect Biochem.Molec.Biol.,14(3):243-260.
Chen AD,Li KB,Yin J,Cao YZ,2011.Effects of environmental factors on the respiratory metabolism in larvae of Pleonomus canaliculatus(Coleoptera:Elateridae).Acta Entomol.Sin.,54(4):397-403.[陈爱端,李克斌,尹姣,曹雅忠,2011.环境因子对沟金针虫呼吸代谢的影响.昆虫学报,54(4):397-403]
Chen B,Kang L,2005.Insect adaptation to environmental temperature stress and population differentiation.Prog.Nat.Sci.,15(3):265-271.[陈兵,康乐,2005.昆虫对环境温度胁迫的适应与种群分化.自然科学进展,15(3):265-271]
Duan YF,Dong HB,Wang Y,Li ZJ,Zhang JS,2016.Respiratory metabolic enzyme activities and RNA/DNA ratio of Marsupenaeus japonicus after desiccation.Marine Fish.,38(1):42-50.[段亚飞,董宏标,王芸,李卓佳,张家松,2016.干露胁迫对日本囊对虾呼吸代谢酶活性和RNA/DNA比值的影响.海洋渔业,38(1):42-50]
Gao D,1998.A preliminary study on the origin of Riptortus pedestris Fabricius.Fujian Agric.Sci.Tech.,(2):16.[高岱,1998.点蜂缘蝽为害莲子的初步研究.福建农业科技,(2):16]
Gao F,Su JW,Ge F,Wu G,Liu XH,2007.Effect of temperature on the respiration and metabolism of ladybeetles,Propylaea japonica.Hubei Agric.Sci.,46(4):562-564.[高峰,苏建伟,戈峰,吴刚,刘向辉,2007.温度对龟纹瓢虫呼吸代谢的影响.湖北农业科学,46(4):562-564]
Gao XL,Zhang M,Tian HQ,Zheng JM,Li X,Song CB,Liu Y,2016.Effect of LED light quality on respiratory metabolism and activities of related enzymes of Haliotis discus hannai.Aquaculture,452:52-61.
Ge F,1991.Factors affecting insect respiratory metabolism.Entomol.Knowl.,28(5):319-321.[戈峰,1991.影响昆虫呼吸代谢的因素.昆虫知识,28(5):319-321]
Guo AG,2009.Basic Biochemistry.Higher Education Press,Beijing.165.[郭蔼光,2009.基础生物化学.北京:高等教育出版社.165]
Guo CH,Yang SR,Yang ZD,Wang RR,Wu G,Zhao PF,2017.Effects of fenvalerate and phoxim on respiratory metabolism,detoxification enzymes and protective enzymes activity of the 4th instar larvae of Diaphania angustalis.China Plant Prot.,37(10):19-22.[郭春晖,杨士芮,杨振德,王蕊蕊,吴广,赵鹏飞,2017.氰戊·辛硫磷对绿翅绢野螟4龄幼虫呼吸代谢、解毒酶及保护酶活性的影响.中国植保导刊,37(10):19-22]
Han LZ,Zhai BP,Zhang XX,Liu PL,2005.Activity of enzyme related to energy metabolism in flight muscles of beet armyworm.Acta Ecol.Sin.,25(5):1101-1106.[韩兰芝,翟保平,张孝羲,刘培磊,2005.甜菜夜蛾飞行肌中与能量代谢有关的酶活性.生态学报,25(5):1101-1106]
Hou Y,Wang XL,Saha TT,Roy S,Zhao B,Raikhe AS,Zou Z,2015.Temporal coordination of carbohydrate metabolism during mosquito reproduction.PLoS Genet.,11(7):e1005309.
Jia HC,2008.Occurrence and control of Riptortus pedestris Fabricius.Xiandai Horticult.,(7):30.[贾慧春,2008.菜豆点蜂缘蝽的发生与防治.现代园艺,(7):30]
Kim E,Park CG,Lim UT,2014.Evaluation of three plant seeds as potential pre-season diets for Riptortus pedestris.J.Asia-Pacific Entomol.,17(3):521-524.
Li J,Li S,Wang DM,Ji R,2014.Changes in the contents of stress resistant substances in Gomphocerus sibiricus(Orthoptera:Acrididae)under high temperature stress.Acta Entomol.Sin.,57(10):1155-1161.[李娟,李爽,王冬梅,季荣,2014.高温胁迫下西伯利亚蝗体内抗逆物质含量变化.昆虫学报,57(10):1155-1161]
Liu YQ,Li XH,Zhao JR,Wang ZJ,2017.Effect of intestinal airbreathing restriction on respiratory metabolism and antioxidant capability of loach(Paramisgurnus dabryanus).Chin.J.Zool.,52(5):857-864.[刘亚秋,李新辉,赵健蓉,王志坚,2017.肠呼吸抑制胁迫对大鳞副泥鳅呼吸代谢和抗氧化能力的影响.动物学杂志,52(5):857-864]
Liu Z,Zeng L,2017.Respiratory metabolism of Bactrocera dorsalis larvae.Anhui Agric.Sci.,45(22):104-106.[刘珍,曾玲,2017.桔小实蝇幼虫呼吸代谢研究.安徽农业科学,45(22):104-106]
Marron MT,Markow TA,Kain KJ,Gibbs AG,2003.Effects of starvation and desiccation on energy metabolism in desert and mesic Drosophila.J.Insect Physiol.,49(3):261-270.
Nie HT,Li WH,Li DD,Li DL,Yan XW,2018.Effects of temperature and salinity on activity of respiratory metabolism-related enzymes in cockle clam Clinocardium californiense.J.Econ.Anim.,22(2):85-89,95.[聂鸿涛,李文昊,李东东,李东霖,闫喜武,2018.温度和盐度对加州扁鸟蛤(Clinocardium californiense)呼吸代谢酶活性的影响.经济动物学报,22(2):85-89,95]
Pang XF,1963.Effect of temperature on oxygen uptake of several insects.Entomol.Knowl.,(2):56-63.[庞雄飞,1963.温度对几种昆虫吸氧量的影响.昆虫知识,(2):56-63]
Qi YY,Zhao CX,Shao WX,Cui SY,Zhang GZ,Hua JX,2017.Occurrence and control techniques of Riptortus pedestris Fabricius in soybean spot in Langfang area.Modern Rural Sci.Tech.,(9):34.[齐永悦,赵春霞,邵维仙,崔绍玉,张桂珍,华建鑫,2017.廊坊地区大豆点蜂缘蝽的发生与防治技术.现代农村科技,(9):34]
Qian X,Wang DM,Li S,Dou J,Ji R,2016.Seasonal variation in respiratory metabolism and its adaptive value in Pyrrhocoris apterus.Acta Ecol.Sin.,36(20):6602-6606.[钱雪,王冬梅,李爽,窦洁,季荣,2016.始红蝽呼吸代谢的季节变化及对温度的适应性.生态学报,36(20):6602-6606]
Qian X,Wang YY,Xie HH,Dou J,Li ZW,Jashhenko R,Ji R,2017.Effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of Gomphocerus sibiricus(Orthoptera:Acrididae).Acta Entomol.Sin.,60(5):499-504.[钱雪,王月莹,谢欢欢,窦洁,李占武,Jashenko R,季荣,2017.温度对西伯利亚蝗呼吸代谢关键酶活性的影响.昆虫学报,60(5):499-504]
Wang DM,Li J,Li S,Hu HX,Ji R,2014.Effects of temperature on the respiratory metabolism of Calliptamus italicus(Orthoptera:Acrididae).Acta Entomol.Sin.,57(3):373-378.[王冬梅,李娟,李爽,扈鸿霞,季荣,2014.温度对意大利蝗呼吸代谢的影响.昆虫学报,57(3):373-378]
Wang J,Li BL,Wu JX,Xu XL,2016.Effects of fluctuating temperature on the reproduction and metabolism of primary energy substances in Mythimna separata(Lepidoptera:Noctuidae).Acta Entomol.Sin.,59(9):917-924.[王娟,李伯辽,仵均祥,许向利,2016.变温对粘虫生殖及主要能源物质代谢的影响.昆虫学报,59(9):917-924]
Wang MQ,Li ZZ,2001.Respiratory metabolism during the larval stage of the cypress sawfly,Chinolyda flagellicornis.Acta Entomol.Sin.,44(3):311-315.[王满囷,李周直,2001.鞭角华扁叶蜂幼虫期的呼吸代谢.昆虫学报,44(3):311-315]
Wang YC,2004.Insect Physiology.China Agriculture Press,Beijing.116-123.[王荫长,2004.昆虫生理学.北京:中国农业出版社.116-123]
Wang YM,Wu JX,Wan FH,2010.Response of insects to extremely high and low temperature stress.J.Environ.Entomol.,32(2):250-255.[王艳敏,仵均祥,万方浩,2010.昆虫对极端高低温胁迫的响应研究.环境昆虫学报,32(2):250-255]
Wang ZD,2013.Identification and control of major sucking pests in soybean.Agric.Tech.Serv.,30(7):711,713.[王正东,2013.大豆主要刺吸害虫的识别与防治.农技服务,30(7):711,713]
Wasser J,2017.Every breath you take:physiology and the ecology of knowing in meditative practice.Int.J.Dhar.Stud.,5:2.
Xiao D,Tan X,Wang W,Zhang F,Desneux N,Wang S,2017.Modification of flight and locomotion performances,respiratory metabolism,and transcriptome expression in the lady beetle Harmonia axyridis through sublethal pesticide exposure.Front.Physiol.,8:33.
Xie H,Chen LJ,Han J,Wang C,2016.Damage characteristics and control methods of Riptortus pedestris in soybean.Soybean Sci.Tech.,(6):11-13.[谢皓,陈立军,韩俊,王程,2016.大豆害虫点蜂缘蝽的危害特点与防治方法.大豆科技,(6):11-13]
Yuan RL,Wang XW,Yang S,Chen P,2015.Changes in the activities of enzymes related to energy metabolism in flight muscles of adult Bactrocera dorsalis(Diptera:Tephritidae)at different ages and during tethered flight.Acta Entomol.Sin.,58(5):471-478.[袁瑞玲,王晓渭,杨珊,陈鹏,2015.不同日龄和吊飞过程中桔小实蝇成虫飞行肌能量代谢相关酶活性的变化.昆虫学报,58(5):471-478]
Zhang J,Qin XW,Zheng X,Dong JH,Zhang ZK,2013.Effects of SRBSDV-infected rice on the accumulation of energy substances of the virus vector,Sogatella furcifera(Horváth).J.Environ.Entomol.,35(5):597-602.[张洁,秦小娃,郑雪,董家红,张仲凯,2013.饲食感染SRBSDV的水稻病株对传毒介体白背飞虱相关能源物质含量的影响.环境昆虫学报,35(5):597-602]
Zhang L,2014.Identification and control of the common soybean insect pests.J.Agric.Catastrophol.,4(12):21-23,28.[张雷,2014.大豆常见虫害的识别与防治.农业灾害研究,4(12):21-23,28]
Zhang WQ,Li YT,2017.The control method of Riptortus pedestris Fabricius damage in Beijing suburbs.Jilin Agric.,(9):80.[张文强,李元涛,2017.京郊大豆点蜂缘蝽虫害防治方法.吉林农业,(9):80]
Bale JS,Masters GJ,Hodkinson ID,Awmack C,Bezemer TM,Brown VK,Butterfield J,Buse A,Coulson JC,Farrar J,Good JEG,Harrington R,Hartley S,Jones TH,Lindroth RL,Prss MC,Symrnioudis I,Watt AD,Whittaker J,2002.Herbivory in global climate change research:direct effects of rising temperature on insect herbivores.Glob.Chan.Biol.,8(1):1-16.
Beenakkers AMT,1969.Carbohydrate and fat as a fuel for insect flight:a comparative study.J.Insect Physiol.,15:353-361.
Beenakkers AMT,Van der Horst DJ,Van Marrewijk WJA,1984.Insect flight muscle metabolism.Insect Biochem.Molec.Biol.,14(3):243-260.
Chen AD,Li KB,Yin J,Cao YZ,2011.Effects of environmental factors on the respiratory metabolism in larvae of Pleonomus canaliculatus(Coleoptera:Elateridae).Acta Entomol.Sin.,54(4):397-403.[陈爱端,李克斌,尹姣,曹雅忠,2011.环境因子对沟金针虫呼吸代谢的影响.昆虫学报,54(4):397-403]
Chen B,Kang L,2005.Insect adaptation to environmental temperature stress and population differentiation.Prog.Nat.Sci.,15(3):265-271.[陈兵,康乐,2005.昆虫对环境温度胁迫的适应与种群分化.自然科学进展,15(3):265-271]
Duan YF,Dong HB,Wang Y,Li ZJ,Zhang JS,2016.Respiratory metabolic enzyme activities and RNA/DNA ratio of Marsupenaeus japonicus after desiccation.Marine Fish.,38(1):42-50.[段亚飞,董宏标,王芸,李卓佳,张家松,2016.干露胁迫对日本囊对虾呼吸代谢酶活性和RNA/DNA比值的影响.海洋渔业,38(1):42-50]
Gao D,1998.A preliminary study on the origin of Riptortus pedestris Fabricius.Fujian Agric.Sci.Tech.,(2):16.[高岱,1998.点蜂缘蝽为害莲子的初步研究.福建农业科技,(2):16]
Gao F,Su JW,Ge F,Wu G,Liu XH,2007.Effect of temperature on the respiration and metabolism of ladybeetles,Propylaea japonica.Hubei Agric.Sci.,46(4):562-564.[高峰,苏建伟,戈峰,吴刚,刘向辉,2007.温度对龟纹瓢虫呼吸代谢的影响.湖北农业科学,46(4):562-564]
Gao XL,Zhang M,Tian HQ,Zheng JM,Li X,Song CB,Liu Y,2016.Effect of LED light quality on respiratory metabolism and activities of related enzymes of Haliotis discus hannai.Aquaculture,452:52-61.
Ge F,1991.Factors affecting insect respiratory metabolism.Entomol.Knowl.,28(5):319-321.[戈峰,1991.影响昆虫呼吸代谢的因素.昆虫知识,28(5):319-321]
Guo AG,2009.Basic Biochemistry.Higher Education Press,Beijing.165.[郭蔼光,2009.基础生物化学.北京:高等教育出版社.165]
Guo CH,Yang SR,Yang ZD,Wang RR,Wu G,Zhao PF,2017.Effects of fenvalerate and phoxim on respiratory metabolism,detoxification enzymes and protective enzymes activity of the 4th instar larvae of Diaphania angustalis.China Plant Prot.,37(10):19-22.[郭春晖,杨士芮,杨振德,王蕊蕊,吴广,赵鹏飞,2017.氰戊·辛硫磷对绿翅绢野螟4龄幼虫呼吸代谢、解毒酶及保护酶活性的影响.中国植保导刊,37(10):19-22]
Han LZ,Zhai BP,Zhang XX,Liu PL,2005.Activity of enzyme related to energy metabolism in flight muscles of beet armyworm.Acta Ecol.Sin.,25(5):1101-1106.[韩兰芝,翟保平,张孝羲,刘培磊,2005.甜菜夜蛾飞行肌中与能量代谢有关的酶活性.生态学报,25(5):1101-1106]
Hou Y,Wang XL,Saha TT,Roy S,Zhao B,Raikhe AS,Zou Z,2015.Temporal coordination of carbohydrate metabolism during mosquito reproduction.PLoS Genet.,11(7):e1005309.
Jia HC,2008.Occurrence and control of Riptortus pedestris Fabricius.Xiandai Horticult.,(7):30.[贾慧春,2008.菜豆点蜂缘蝽的发生与防治.现代园艺,(7):30]
Kim E,Park CG,Lim UT,2014.Evaluation of three plant seeds as potential pre-season diets for Riptortus pedestris.J.Asia-Pacific Entomol.,17(3):521-524.
Li J,Li S,Wang DM,Ji R,2014.Changes in the contents of stress resistant substances in Gomphocerus sibiricus(Orthoptera:Acrididae)under high temperature stress.Acta Entomol.Sin.,57(10):1155-1161.[李娟,李爽,王冬梅,季荣,2014.高温胁迫下西伯利亚蝗体内抗逆物质含量变化.昆虫学报,57(10):1155-1161]
Liu YQ,Li XH,Zhao JR,Wang ZJ,2017.Effect of intestinal airbreathing restriction on respiratory metabolism and antioxidant capability of loach(Paramisgurnus dabryanus).Chin.J.Zool.,52(5):857-864.[刘亚秋,李新辉,赵健蓉,王志坚,2017.肠呼吸抑制胁迫对大鳞副泥鳅呼吸代谢和抗氧化能力的影响.动物学杂志,52(5):857-864]
Liu Z,Zeng L,2017.Respiratory metabolism of Bactrocera dorsalis larvae.Anhui Agric.Sci.,45(22):104-106.[刘珍,曾玲,2017.桔小实蝇幼虫呼吸代谢研究.安徽农业科学,45(22):104-106]
Marron MT,Markow TA,Kain KJ,Gibbs AG,2003.Effects of starvation and desiccation on energy metabolism in desert and mesic Drosophila.J.Insect Physiol.,49(3):261-270.
Nie HT,Li WH,Li DD,Li DL,Yan XW,2018.Effects of temperature and salinity on activity of respiratory metabolism-related enzymes in cockle clam Clinocardium californiense.J.Econ.Anim.,22(2):85-89,95.[聂鸿涛,李文昊,李东东,李东霖,闫喜武,2018.温度和盐度对加州扁鸟蛤(Clinocardium californiense)呼吸代谢酶活性的影响.经济动物学报,22(2):85-89,95]
Pang XF,1963.Effect of temperature on oxygen uptake of several insects.Entomol.Knowl.,(2):56-63.[庞雄飞,1963.温度对几种昆虫吸氧量的影响.昆虫知识,(2):56-63]
Qi YY,Zhao CX,Shao WX,Cui SY,Zhang GZ,Hua JX,2017.Occurrence and control techniques of Riptortus pedestris Fabricius in soybean spot in Langfang area.Modern Rural Sci.Tech.,(9):34.[齐永悦,赵春霞,邵维仙,崔绍玉,张桂珍,华建鑫,2017.廊坊地区大豆点蜂缘蝽的发生与防治技术.现代农村科技,(9):34]
Qian X,Wang DM,Li S,Dou J,Ji R,2016.Seasonal variation in respiratory metabolism and its adaptive value in Pyrrhocoris apterus.Acta Ecol.Sin.,36(20):6602-6606.[钱雪,王冬梅,李爽,窦洁,季荣,2016.始红蝽呼吸代谢的季节变化及对温度的适应性.生态学报,36(20):6602-6606]
Qian X,Wang YY,Xie HH,Dou J,Li ZW,Jashhenko R,Ji R,2017.Effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of Gomphocerus sibiricus(Orthoptera:Acrididae).Acta Entomol.Sin.,60(5):499-504.[钱雪,王月莹,谢欢欢,窦洁,李占武,Jashenko R,季荣,2017.温度对西伯利亚蝗呼吸代谢关键酶活性的影响.昆虫学报,60(5):499-504]
Wang DM,Li J,Li S,Hu HX,Ji R,2014.Effects of temperature on the respiratory metabolism of Calliptamus italicus(Orthoptera:Acrididae).Acta Entomol.Sin.,57(3):373-378.[王冬梅,李娟,李爽,扈鸿霞,季荣,2014.温度对意大利蝗呼吸代谢的影响.昆虫学报,57(3):373-378]
Wang J,Li BL,Wu JX,Xu XL,2016.Effects of fluctuating temperature on the reproduction and metabolism of primary energy substances in Mythimna separata(Lepidoptera:Noctuidae).Acta Entomol.Sin.,59(9):917-924.[王娟,李伯辽,仵均祥,许向利,2016.变温对粘虫生殖及主要能源物质代谢的影响.昆虫学报,59(9):917-924]
Wang MQ,Li ZZ,2001.Respiratory metabolism during the larval stage of the cypress sawfly,Chinolyda flagellicornis.Acta Entomol.Sin.,44(3):311-315.[王满囷,李周直,2001.鞭角华扁叶蜂幼虫期的呼吸代谢.昆虫学报,44(3):311-315]
Wang YC,2004.Insect Physiology.China Agriculture Press,Beijing.116-123.[王荫长,2004.昆虫生理学.北京:中国农业出版社.116-123]
Wang YM,Wu JX,Wan FH,2010.Response of insects to extremely high and low temperature stress.J.Environ.Entomol.,32(2):250-255.[王艳敏,仵均祥,万方浩,2010.昆虫对极端高低温胁迫的响应研究.环境昆虫学报,32(2):250-255]
Wang ZD,2013.Identification and control of major sucking pests in soybean.Agric.Tech.Serv.,30(7):711,713.[王正东,2013.大豆主要刺吸害虫的识别与防治.农技服务,30(7):711,713]
Wasser J,2017.Every breath you take:physiology and the ecology of knowing in meditative practice.Int.J.Dhar.Stud.,5:2.
Xiao D,Tan X,Wang W,Zhang F,Desneux N,Wang S,2017.Modification of flight and locomotion performances,respiratory metabolism,and transcriptome expression in the lady beetle Harmonia axyridis through sublethal pesticide exposure.Front.Physiol.,8:33.
Xie H,Chen LJ,Han J,Wang C,2016.Damage characteristics and control methods of Riptortus pedestris in soybean.Soybean Sci.Tech.,(6):11-13.[谢皓,陈立军,韩俊,王程,2016.大豆害虫点蜂缘蝽的危害特点与防治方法.大豆科技,(6):11-13]
Yuan RL,Wang XW,Yang S,Chen P,2015.Changes in the activities of enzymes related to energy metabolism in flight muscles of adult Bactrocera dorsalis(Diptera:Tephritidae)at different ages and during tethered flight.Acta Entomol.Sin.,58(5):471-478.[袁瑞玲,王晓渭,杨珊,陈鹏,2015.不同日龄和吊飞过程中桔小实蝇成虫飞行肌能量代谢相关酶活性的变化.昆虫学报,58(5):471-478]
Zhang J,Qin XW,Zheng X,Dong JH,Zhang ZK,2013.Effects of SRBSDV-infected rice on the accumulation of energy substances of the virus vector,Sogatella furcifera(Horváth).J.Environ.Entomol.,35(5):597-602.[张洁,秦小娃,郑雪,董家红,张仲凯,2013.饲食感染SRBSDV的水稻病株对传毒介体白背飞虱相关能源物质含量的影响.环境昆虫学报,35(5):597-602]
Zhang L,2014.Identification and control of the common soybean insect pests.J.Agric.Catastrophol.,4(12):21-23,28.[张雷,2014.大豆常见虫害的识别与防治.农业灾害研究,4(12):21-23,28]
Zhang WQ,Li YT,2017.The control method of Riptortus pedestris Fabricius damage in Beijing suburbs.Jilin Agric.,(9):80.[张文强,李元涛,2017.京郊大豆点蜂缘蝽虫害防治方法.吉林农业,(9):80]