摘要
以福贡龙竹种子为外植体,开展组培快繁技术研究。结果表明,用75%酒精消毒60s,再用0.1%的Hg Cl2溶液消毒30min的效果最好;适宜诱导形成丛生芽的培养基为MS+6-BA 2mg/L+IBA 0.5mg/L+KT 0.2mg/L;最佳继代增殖培养基为MS+6-BA 3mg/L+NAA 0.2mg/L;以MS+IBA 1mg/L+NAA 0.5mg/L为最佳生根培养基;将生根组培苗炼苗移栽,90d后成活率达81%以上。
The tissue culture for Dendrocalams fugongensis was conducted using its seeds as explants. The results showed that disinfection for 60 s with 75% alcohol and disinfection for 30 min with 0. 1% Hg Cl2 were most effective approach. The optimal multiple shoots inducing medium was MS + 6-BA 2 mg/L + IBA 0. 5 mg/L + KT 0. 2 mg/L.The optimal shoots multiplication culture medium was MS+6-BA 3 mg/L +NAA 0. 2 mg/L. The optimal rooting culture medium was MS+IBA 1 mg/L +NAA 0. 5 mg/L. After 90 days transplanted into greenhouse,the survival rate of young plants were over 81%.
引文
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