食品中果聚糖的测定方法研究进展
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摘要
果聚糖天然存在于菊苣、菊芋、香蕉、大蒜、洋葱、大麦等多种植物中,作为一种水溶性膳食纤维广泛应用于婴幼儿食品、乳制品、饮料、焙烤食品等,且果聚糖具有与普通食糖迥然不同的消化过程和代谢途径,并具有多种生理功能,一直是国内外生产企业和科研院所关注的热点。由于果聚糖的聚合度不同,且存在不同的化学结构形式,一些明确化学结构式的成分缺少对照品,因此定性定量分析有很大的难度。近年来,国内外科研人员在分析检测方面做出了不懈的努力,建立了多种检测方法。本文对果聚糖的分类、结构和应用进行了概述,并总结了各种检测方法的优缺点,为生产企业、检验检测机构人员选择检测方法提供技术支持,为精准定量食品中的果聚糖含量提供参考。
Fructans are naturally found in some kinds of plants such as witloof, jerusalem artichoke, banana,garlic, onion and barley naturally. As a kind of soluble dietary fiber, fructans are applied to infant food, dairy products, beverages, bakery products intensively, and compared with ordinary sugar, fructans have utterly different digestive process and metabolic pathways, and have kinds of physiological function, which are always hot spots for domestic and foreign manufacturers and research institutes. Because of different polymerization degree of fructans,and the existence of different chemical structure forms, some precise chemical structures lack of reference substance,the qualitative and quantitative analysis of fructans is very difficult. In recent years, researchers at home and abroad have made unremitting efforts in analysis and testing, and established a variety of testing methods. The paper summarized the classification, chemical structure, application of fructans and their merits and demerits of various detection methods, which could provide technical support when choose detection method for manufacturing enterprise and personnel of inspection and testing institutions, which could also serve accurate determination for fructans.
Fructans are naturally found in some kinds of plants such as witloof, jerusalem artichoke, banana,garlic, onion and barley naturally. As a kind of soluble dietary fiber, fructans are applied to infant food, dairy products, beverages, bakery products intensively, and compared with ordinary sugar, fructans have utterly different digestive process and metabolic pathways, and have kinds of physiological function, which are always hot spots for domestic and foreign manufacturers and research institutes. Because of different polymerization degree of fructans,and the existence of different chemical structure forms, some precise chemical structures lack of reference substance,the qualitative and quantitative analysis of fructans is very difficult. In recent years, researchers at home and abroad have made unremitting efforts in analysis and testing, and established a variety of testing methods. The paper summarized the classification, chemical structure, application of fructans and their merits and demerits of various detection methods, which could provide technical support when choose detection method for manufacturing enterprise and personnel of inspection and testing institutions, which could also serve accurate determination for fructans.
引文
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[12] Rikita T, Egawa Y, Seki T. Fluorometric determination of inulin using5-quinolineboronic acid and inulinase[J]. Anal Biochem, 2012,(426):24-26.
[13] Saengkanuk A, Nuchadomrong S, Jogloy S, et al. A simplified spectrophotometric method for the determinationof inulin in Jerusalem artichoke(Helianthus tuberosus L.)tubers[J]. Eur Food Res Technol,2011,233(4):609-616.
[14]吴洪新,单昌辉,李薇,等.紫外分光光度计法测定菊粉多糖[J].安徽农业科学,2008, 36(13):5251-5253.Wu HX, Shan CH, Li W, et al. Study on the detection of inulin by the method of UV-VIS spectrophotometry[J]. J Anhui Agric Sci, 2008,36(13):5251-5253.
[15] AOAC 999.03 Measurement of total fructan in foods enzymatic/spectrophotometric method[S].
[16]耿丽娟.四类食品中低聚果糖的检测技术研究[D].西安:陕西科技大学,2015.Geng LJ. Study on the detection technique of fructooligosaccharides in four kinds of foods[D]. Xi'an:Shaanxi University of Science&Technology, 2015.
[17]邓伟恒,藜韵,吴国辉.等.配方乳粉中低聚果糖的含量测定方法比较[J].分析检测,2018,(10):135-137.Deng WH, Li Y, Wu GH, et al. Comparison of determination methods of fructo-oligosaccharides in formula milk powder[J]. Anal Test, 2018,(10):135-137.
[18] Vendrell-Pascuas S, Castellote-Bargallo'AI, Lo'pez-Sabater MC.Determination of inulin in meat products by high-performance liquid chromatography with refractive index detection[J].J Chromatogr A, 2000,(881):591-597.
[19]张媛媛,张彬,韩爱云,等.三种低聚果糖检测方法的比较[J].食品研究与开发,2015, 36(14):98-101.Zhang YY, Zhang B, Han AY, et al. Comparison of three methods for determination of fructooligosaccharides[J]. Food Res Dev, 2015, 36(14):98-101.
[20] Kristo E, Foo A, Hill AR, et al. Short communication:Determination of inulin in milk using high-performance liquid chromatography with evaporative light scattering detection[J]. Dairy Sci, 2011,(94):3316-3321.
[21]黄晓林,宋戈,张丽宏,等.乳制品中单糖、双糖和低聚果糖的同时测定[J].中国乳品工业,2017,45(11):37-39.Huang XL, Song G, Zhang LH, et al. Simultaneous determination of monosaccharides, disaccharides and oligosaccharides in dairy products[J].China Dairy Ind,2017,45(11):37-39.
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