摘要
对机采棉产量和品质性状的数量性状基因座(QTL)进行定位,为培育高产优质新品种提供理论依据。本研究基于陆地棉‘中571’与‘中棉所49’构建的包括3 187个Bin标记的高密度遗传图谱,利用F2∶3群体田间表型数据,采用复合区间作图法(CIM),对产量和品质性状的QTL进行检测。结果表明:共检测到产量性状10个QTL,品质性状10个QTL。其中,产量性状中控制铃重QTL 5个,衣分QTL 1个,产量相关指标子指QTL4个,分布在2、8、15、17和20号染色体上,QTL区段中LOD值介于4.61~6.33,解释表型变异比例为2.93%~13.67%;品质性状中控制纤维上半部平均长度QTL 3个,断裂比强度QTL 2个,马克隆值QTL 5个,分布在1、2、15、23和25号染色体上,QTL区段中LOD值介于3.76~8.35,解释表型变异比例为2.97%~11.78%。此外,控制铃重的主效qBW-chr08-1,子指主效QTL qSI-chr15-1,纤维上半部平均长度主效QTLqFLq-chr15-1位点可用于精细定位和克隆。
The study was conducted to locate QTLs of fiber yield and quality traits in the crossing population of cotton,in order to provide a theoretical basis for cultivating high-yielding and quality varieties.Based on the high-density genetic map with 3 187 Bin markers constructed by‘Z571'and ‘CCRI 49'in upland cotton,the QTLs for yield and quality traits were detected by using composite interval mapping(CIM)with field phenotype data of F2∶3 population.The results indicated that 10 QTLs for yield traits and 10 QTLs for quality traits were detected.Among the yield traits,5 QTLs for controlling boll weight(BW),1 QTLs for lint percentage(LP),and 4 QTLs for seed index(SI)were located on chromosomes 2,8,15,17,and 20.The LOD scores of QTLs ranged from 4.61 to 6.33,explaining the phenotypic variation ranging from 2.93% to13.67%.3 QTLs for mean length of the upper half fiber(FL),2 QTLs for breaking tenacity of the fiber(FT),and 5 QTLs for the micronaire(Mc)were located on chromosomes 1,2,15,23 and 25.The LOD scores of QTLs ranged from 3.76 to 8.35,explaining the phenotypic variation ranging from 2.97% to 11.78%.In addition,qBW-chr8-3 amajor QTL for BW,qSI-chr15-1 amajor QTL for SI,and qFLq-chr15-1 amajor QTL for FL could be used for fine mapping and cloning.
引文
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