秸秆源品质改良因子处理对黄桃果实抗病性及贮藏品质的影响
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摘要
以"八月黄"黄桃果实为材料,用3%秸秆源品质改良因子对黄桃叶片进行采前喷施处理,研究其对采后黄桃果实抗病性及贮藏品质的的影响。结果表明:3%秸秆源品质改良因子采前处理可以降低接种灰霉黄桃果实的病斑面积及乙烯释放量,显著提高病程相关基因NPR1、PR1的表达量,激活抗病酶β-1,3-葡聚糖酶(GLU)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)和几丁质酶(CHI)的活性,从而提高黄桃果实的抗病性。此外,3%秸秆源品质改良因子采前处理可显著提高果实中的可溶性糖、可溶性蛋白的含量,从而改善果实的贮藏品质。
‘August yellow' peach fruit was treated before harvest to study the effect of 3% stalk-derived quality modification factor on peach disease resistance and storage quality. The results showed that, 3% stalk-derived quality modification factor reduced the area of disease spot and ethylene production, enhanced the gene expression of NPR1(non-expressor of pathogenesis-related genes 1) and PR1(pathogenesis-related genes 1), increased enzyme activaty of GLU(β-1,3-glucanase), PAL(phenylalanine ammonialyase), PPO(polyphenol oxidase) and CHI(chitinase), thereby enhancing the disease resistance of peach fruit. In addition, 3% stalk-derived quality modification factor increased soluble protein and soluble sugar content, resulting in improved storage quality of peach fruit.
‘August yellow' peach fruit was treated before harvest to study the effect of 3% stalk-derived quality modification factor on peach disease resistance and storage quality. The results showed that, 3% stalk-derived quality modification factor reduced the area of disease spot and ethylene production, enhanced the gene expression of NPR1(non-expressor of pathogenesis-related genes 1) and PR1(pathogenesis-related genes 1), increased enzyme activaty of GLU(β-1,3-glucanase), PAL(phenylalanine ammonialyase), PPO(polyphenol oxidase) and CHI(chitinase), thereby enhancing the disease resistance of peach fruit. In addition, 3% stalk-derived quality modification factor increased soluble protein and soluble sugar content, resulting in improved storage quality of peach fruit.
引文
[1]金鹏.桃果实采后病害和冷害调控及其机理研究[D].南京:南京农业大学, 2009.
[2] WILSON C L, WISNIEWSKI M E. Biological control of postharvest diseases of fruits and vegetables:An emerging technology[J]. Annual Review of Phytopathology, 1989, 27(27):425-441.
[3]任文明,毋智深,高爱武.桃保鲜技术研究[J].内蒙古农牧学院学报, 1999, 2(1):78-82.
[4]杜建厂,马凯,王兴娜.桃贮藏保鲜技术研究进展[J].北方果树, 2000(6):1-3.
[5]刘彩莉,霍君生.贮藏条件对桃果实的生理影响[J].河北农业大学学报, 1992, 15(l):88-91.
[6]朱漩.天然中草药果蔬防腐保鲜剂的研究进展[J].新疆农业科学, 2004, 41(s1):110-111.
[7]王瑞祥.利用天然植物提取液对蔬果防腐保鲜的实验[J].食品与健康, 1996(3):42-43.
[8]甘霖,申琳,生吉萍.秸秆源品质改良因子采前处理对番茄果实品质的影响[J].食品科学, 2014, 34(4):221-223.
[9]葛佳,李博,李然然,等.叶面喷施秸秆源提取物对草莓果实产量和品质的影响[J].食品科学, 2014, 35(6):200-203.
[10] FAN B, SHEN L, LIU K L, et al. Interaction between nitric oxide and hydrogen peroxide in postharvest tomato resistance response to Rhizopus nigricans[J]. Journal of the Science of Food and Agriculture, 2008, 88(7):1238-1244.
[11] ZUCKER M. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue[J]. Plant Physiology, 1965, 40(5):779-784.
[12] BOLLER T, GEHRI A, MAUCH F, et al. Chitinase in bean leaves:induction by ethylene, purification, properties, and possible function[J]. Planta, 1983, 157(1):22-31.
[13] ABELES F B, FORRENCE L E. Temporal and hormonal control ofβ-1,3-glucanase in Phaseolus vulgaris L[J].Plant Physiology, 1970, 45(4):395-400.
[14] ZAUBERMAN G, RONEN R, AKERMAN M. Postharvest retention of the red color of litchi fruit pericarp[J]. Scientia Horticulturae, 1991, 47(1):89-97.
[15] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCTmethod[J]. Methods, 2001, 25(4):402-408.
[16]聂继云.食品质量安全分析技术[M].北京:化学工业出版社, 2009:10-17.
[17]张妙霞,孔祥生,郭秀璞,等.蒽酮法测定可溶性糖显色条件的研究[J].洛阳农专学, 1997, 17(4):24-28.
[18]曹雪慧,杨方威,李青,等.壳聚糖及其复配对大久保桃常温贮藏特性的影响[J].中国食品学报, 2014, 14(4):164-169.
[19]邓丽莉,潘晓倩,生吉萍,等.考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化[J].食品科学, 2012,33(24):185-189.
[20] JIANG W B, MAYAK S, HALEVY A H. The mechanism involved in ethylene-enhanced ethylene synthesis in carnations[J]. Plant Growth Regulation, 1994, 14(2):133-138.
[2] WILSON C L, WISNIEWSKI M E. Biological control of postharvest diseases of fruits and vegetables:An emerging technology[J]. Annual Review of Phytopathology, 1989, 27(27):425-441.
[3]任文明,毋智深,高爱武.桃保鲜技术研究[J].内蒙古农牧学院学报, 1999, 2(1):78-82.
[4]杜建厂,马凯,王兴娜.桃贮藏保鲜技术研究进展[J].北方果树, 2000(6):1-3.
[5]刘彩莉,霍君生.贮藏条件对桃果实的生理影响[J].河北农业大学学报, 1992, 15(l):88-91.
[6]朱漩.天然中草药果蔬防腐保鲜剂的研究进展[J].新疆农业科学, 2004, 41(s1):110-111.
[7]王瑞祥.利用天然植物提取液对蔬果防腐保鲜的实验[J].食品与健康, 1996(3):42-43.
[8]甘霖,申琳,生吉萍.秸秆源品质改良因子采前处理对番茄果实品质的影响[J].食品科学, 2014, 34(4):221-223.
[9]葛佳,李博,李然然,等.叶面喷施秸秆源提取物对草莓果实产量和品质的影响[J].食品科学, 2014, 35(6):200-203.
[10] FAN B, SHEN L, LIU K L, et al. Interaction between nitric oxide and hydrogen peroxide in postharvest tomato resistance response to Rhizopus nigricans[J]. Journal of the Science of Food and Agriculture, 2008, 88(7):1238-1244.
[11] ZUCKER M. Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue[J]. Plant Physiology, 1965, 40(5):779-784.
[12] BOLLER T, GEHRI A, MAUCH F, et al. Chitinase in bean leaves:induction by ethylene, purification, properties, and possible function[J]. Planta, 1983, 157(1):22-31.
[13] ABELES F B, FORRENCE L E. Temporal and hormonal control ofβ-1,3-glucanase in Phaseolus vulgaris L[J].Plant Physiology, 1970, 45(4):395-400.
[14] ZAUBERMAN G, RONEN R, AKERMAN M. Postharvest retention of the red color of litchi fruit pericarp[J]. Scientia Horticulturae, 1991, 47(1):89-97.
[15] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCTmethod[J]. Methods, 2001, 25(4):402-408.
[16]聂继云.食品质量安全分析技术[M].北京:化学工业出版社, 2009:10-17.
[17]张妙霞,孔祥生,郭秀璞,等.蒽酮法测定可溶性糖显色条件的研究[J].洛阳农专学, 1997, 17(4):24-28.
[18]曹雪慧,杨方威,李青,等.壳聚糖及其复配对大久保桃常温贮藏特性的影响[J].中国食品学报, 2014, 14(4):164-169.
[19]邓丽莉,潘晓倩,生吉萍,等.考马斯亮蓝法测定苹果组织微量可溶性蛋白含量的条件优化[J].食品科学, 2012,33(24):185-189.
[20] JIANG W B, MAYAK S, HALEVY A H. The mechanism involved in ethylene-enhanced ethylene synthesis in carnations[J]. Plant Growth Regulation, 1994, 14(2):133-138.