流式细胞术在急性白血病免疫分型中的应用探讨
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摘要
目的应用多参数流式细胞术检测急性白血病细胞表面CD抗原分子的表达水平,探讨AML和ALL中CD抗原的表达规律,为临床个体化治疗累积分子机制研究材料。方法应用流式细胞术检测44例急性髓细胞白血病患者和18例急性淋巴细胞白血病患者,以CD45/SSC设门,应用13种单克隆抗体对CD45弱表达的细胞群进行免疫分型,测定多种CD分子的表达率,并通过χ~2检验比较不同的高表达CD分子的相关性。结果 44例AML患者中表达且高表达CD33(79.6%),CD38(70.5%),CD117(52.3%),cyt-MPO(50.0%);16例B-ALL患者中CD10(75.0%),CD19(81.25%)以及CD22(81.25%)阳性表达率较高;比较AML患者高表达CD抗原,结果显示CD33/CD38、CD33/cyt-MPO的抗原表达差异均有统计学意义(P<0.05)。结论不同的CD分子在急性白血病细胞上表达有差异性,或将为靶向治疗和临床个体化治疗提供有用的线索。
Objective To detect the expression level of CD antigen on the surface of acute leukemia cell by multiparameter flow cytometry,and to explore the expression principle of CD antigens in AML and ALL,so as to accumulate molecular mechanism research materials for clinical individualized treatment.Methods 44 patients with acute myeloid leukemia and 18 patients with acute lymphoblastic leukemia were detected by flow cytometry.Based on CD45/SSC,13 types of monoclonal antibodies were used to carry out immunophenotype of the cell population with weak expression of CD45,and expression rate of various CD molecules was determined.The χ~2 test was used to compare the correlation of different highly expressed CD molecules.Results CD33(79.6%),CD38(70.5%),CD117(52.3%) and cyt-MPO(50.0%)were highly expressed in 44 patients with AML;the positive rate of CD10(75.0%),CD19(81.25%)and CD22(81.25%)was higher in 16 patients with B-ALL;the high expression of CD antigens in the patients with AML were compared.The results showed that there was a statistically significant difference in the antigen expression of CD33/CD38 and CD33/cyt-MPO(P<0.05).Conclusion Different CD molecules are differentially expressed on acute leukemia cells,or they will provide useful clues for targeted therapy and clinical individualized treatment.
Objective To detect the expression level of CD antigen on the surface of acute leukemia cell by multiparameter flow cytometry,and to explore the expression principle of CD antigens in AML and ALL,so as to accumulate molecular mechanism research materials for clinical individualized treatment.Methods 44 patients with acute myeloid leukemia and 18 patients with acute lymphoblastic leukemia were detected by flow cytometry.Based on CD45/SSC,13 types of monoclonal antibodies were used to carry out immunophenotype of the cell population with weak expression of CD45,and expression rate of various CD molecules was determined.The χ~2 test was used to compare the correlation of different highly expressed CD molecules.Results CD33(79.6%),CD38(70.5%),CD117(52.3%) and cyt-MPO(50.0%)were highly expressed in 44 patients with AML;the positive rate of CD10(75.0%),CD19(81.25%)and CD22(81.25%)was higher in 16 patients with B-ALL;the high expression of CD antigens in the patients with AML were compared.The results showed that there was a statistically significant difference in the antigen expression of CD33/CD38 and CD33/cyt-MPO(P<0.05).Conclusion Different CD molecules are differentially expressed on acute leukemia cells,or they will provide useful clues for targeted therapy and clinical individualized treatment.
引文
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[12]Alan Pomerantz,Sergio Rodríguez-Rodríguez,Roberta Demichelis-Gómez,et al.Importance of CD117 in the assignation of a myeloid lineage in acute leukemias[J].Archives of Medical Research,2017,48(2):364-370.
[13]Degtereva OV,Hancharou A,Ramanava IU,et al.Immunophenotype and subsets of synoviocytes in patients with osteoarthritis[J].Journal of Allergy&Clinical Immunology,2017,139(2):210-216.
[14]Dargart JL,Kahwash SB,Abu-Arja R,et al.Twins diagnosed simultaneously with RAM immunophenotype acute myeloid leukemia[J].Biology of Blood&Marrow Transplantation,2017,23(3):S252-S253.
[15]Farooq T,Islam H,Saeed F,et al.166 A case of atypical lymphoproliferative disorder of NK cells:An aggressive immunophenotype but indolent behavior with normal cytogenetics[J].American Journal of Clinical Pathology,2018,149(suppl_1):S70-S71.
[2]陈英虎,汤永民,沈红强,等.急性白血病细胞中CD19的表达[J].实用肿瘤杂志,2004,1(19):32-35.
[3]金明威,陈哲,陈颖,等.CD7阳性在急性髓细胞白血病中表达的临床意义[J].中国优生与遗传杂志,2010,18(3):36-41.
[4]中华医学会血液学分会.急性髓系白血病(复发难治性)中国诊疗指南(2011年版)[J].中华血液学杂志,2011,9(32):887-888.
[5]范颖.急性髓系白血病复发后生物学标志的变化[J].河北医科大学,2012,11(3):11-16.
[6]郭宏伟,刘涛,王永香.急性淋巴细胞性白血病免疫表型分析[J].宁夏医学杂志,2005,3(7):435-438.
[7]鹿群先,张光彩,李德鹏,等.急性髓细胞白血病免疫表型分析[J].临床内科杂志,2001,15(2):127-129.
[8]张书芹,王光平,朱平,等.急性髓系白血病CD33~+/CD34~+细胞表面核酸适配体的筛选及结构分析[J].中国实验血液学杂志,2011,19(3):561-565.
[9]贾明峰,席亚明,石秀娥,等.MPO、NQO1基因多态性与急性白血病易感性的相关研究[J].中国实验血液学杂志,2012,20(6):1336-1340.
[10]许晓东,胡翠华,常乃柏,等.成人急性髓细胞白血病免疫分型特点及临床预后分析[J].中国实验诊断学,2010,14(3):423-426.
[11]易丽萍,哈力达·亚森.免疫分型及临床应用[J].中国组织工程研究与临床康复,2009,13(5):903-906.
[12]Alan Pomerantz,Sergio Rodríguez-Rodríguez,Roberta Demichelis-Gómez,et al.Importance of CD117 in the assignation of a myeloid lineage in acute leukemias[J].Archives of Medical Research,2017,48(2):364-370.
[13]Degtereva OV,Hancharou A,Ramanava IU,et al.Immunophenotype and subsets of synoviocytes in patients with osteoarthritis[J].Journal of Allergy&Clinical Immunology,2017,139(2):210-216.
[14]Dargart JL,Kahwash SB,Abu-Arja R,et al.Twins diagnosed simultaneously with RAM immunophenotype acute myeloid leukemia[J].Biology of Blood&Marrow Transplantation,2017,23(3):S252-S253.
[15]Farooq T,Islam H,Saeed F,et al.166 A case of atypical lymphoproliferative disorder of NK cells:An aggressive immunophenotype but indolent behavior with normal cytogenetics[J].American Journal of Clinical Pathology,2018,149(suppl_1):S70-S71.