瓜蒌配方颗粒指纹图谱研究
|
摘要
为了全面反映瓜蒌配方颗粒所含多种组分,明确瓜蒌配方颗粒HPLC指纹图谱的测定方法,对10批瓜蒌配方颗粒进行了HPLC指纹图谱测定及相似度评价。采用HPLC方法,色谱柱为GL Sciences公司InertSustain AQ-C_(18)柱(4.6 nm×250 nm,5μm),以甲醇-0.1%甲酸水溶液为流动相,梯度洗脱,检测波长为260 nm,流速为0.8 mL/min,柱温为25℃,对瓜蒌配方颗粒指纹图谱测定结果进行了相似度评价。结果表明,10批瓜蒌配方颗粒指纹图谱中呈现16个共有峰,相似度均大于0.960,表明瓜蒌配方颗粒具有良好的一致性;此外,在16个共有峰中指认出腺嘌呤、尿苷2个化学成分。瓜蒌配方颗粒指纹图谱反映了瓜蒌配方颗粒的多组分面貌,可为瓜蒌配方颗粒的多组分质量控制提供参考依据。
In order to fully reflect the features of various components of Trichosanthis fructus formula granules and clarify the HPLC fingerprint of Trichosanthis fructus formula granules,ten batches of Trichosanthis fructus formula granules are determined by HPLC fingerprint and evaluated for similarity.Using HPLC methed,the chromatographic column is GL Sciences InertSustain AQ-C_(18) column(4.6 nm×250 nm,5 μm),and taking methanol-0.1% formic acid aqueous solution as mobile phase, gradient elution, detection wavelength 260 nm,the flow rate is 0.8 mL/min,and the column temperature is 25 ℃, and the similarity evaluation of Trichosanthis fructus is carried out with reference to fingerprint.The results show that there are 16 common fingerprint peaks in all fingerprint spectra of 10 batches of Trichosanthis fructus formula granules,and the similarity is greater than 0.960,presenting a good similarity.Besides,in the 16 fingerprint peaks, two of them are Adenine and Uridine.Therefore, the fingerprint of Trichosanthis fructus standard decoction reflects the multi-componernt appearance,and the method has good feasibility, which can provide a reference for the multi-componernt quality control of Trichosanthis fructus formula granules.
In order to fully reflect the features of various components of Trichosanthis fructus formula granules and clarify the HPLC fingerprint of Trichosanthis fructus formula granules,ten batches of Trichosanthis fructus formula granules are determined by HPLC fingerprint and evaluated for similarity.Using HPLC methed,the chromatographic column is GL Sciences InertSustain AQ-C_(18) column(4.6 nm×250 nm,5 μm),and taking methanol-0.1% formic acid aqueous solution as mobile phase, gradient elution, detection wavelength 260 nm,the flow rate is 0.8 mL/min,and the column temperature is 25 ℃, and the similarity evaluation of Trichosanthis fructus is carried out with reference to fingerprint.The results show that there are 16 common fingerprint peaks in all fingerprint spectra of 10 batches of Trichosanthis fructus formula granules,and the similarity is greater than 0.960,presenting a good similarity.Besides,in the 16 fingerprint peaks, two of them are Adenine and Uridine.Therefore, the fingerprint of Trichosanthis fructus standard decoction reflects the multi-componernt appearance,and the method has good feasibility, which can provide a reference for the multi-componernt quality control of Trichosanthis fructus formula granules.
引文
[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015.
[2] 孙娟,孟冰雪,赵启韬.瓜蒌药理作用的物质基础研究概况[J].山东中医杂志,2012,31(6):461-463.
[3] 孙兴龙,段崇英.瓜蒌的药理作用及临床应用[J].中国药师杂志,2003,6(10):655-656.
[4] 屠婕红,余菁,陈伟光.瓜蒌的化学成分和药理作用研究概况[J].中国药师,2004,7(7):562-563.
[5] 张腾,张艳军,庄朋伟,等.瓜蒌对心梗后心衰大鼠心功能及心肌细胞凋亡的影响[J].中国药理学通报,2016,32(8):1183-1184.ZHANG Teng,ZHANG Yanjun,ZHUANG Pengwei,et al.Effects of Gualou on cardiac function and cardiomyocyte apoptosis in heart failure following myocardial infarction in rats[J].Chinese Pharmacological Bulletin,2016,32(8):1183-1184.
[6] 张弢,沈文芳,朱俊.瓜蒌对心血管系统的药理作用及临床应用[J].中国乡村医药杂志,2007(11):52.
[7] LI Hui,KOCHUN H,TSANG S Y,et al.The ethanol extract of Trichosanthis fructus promotes fetal hemoglobin production via p38 MAPK activation and ERK inactivation in K562 cells[J].Evidence-Based Complementary and Alternative Medicine,2011:10.1093/ecam/neq022.
[8] YU Xiankuo,TANG Liying,WU Hongwei,et al.Trichosanthis fructus:Botany,traditional uses,phytochemistry and pharmacology[J].Journal of Ethnopharmacology,2018,224:570-578.
[9] 范雪梅,陈刚,苏姗姗,等.瓜蒌化学成分的分离与鉴定[J].沈阳药科大学学报,2011,28(11):871-874.FAN Xuemei,CHEN Gang,SU Shanshan,et al.Isolation and identification of constituents from fruit of Trichosanthes kirilowii Maxim[J].Journal of Shenyang Pharmaceutical University,2011,28(11):871-874.
[10] 万丽娟,卢金清,许俊洁,等.瓜蒌子化学成分和药理作用的研究进展[J].中国药房,2015,26(31):4440-4443.
[11] 孙稚颖,李恩波,周凤琴.山东农家栽培瓜蒌品种的同工酶研究[J].中药材,2005,28(10):4-6.SUN Zhiying,LI Enbo,ZHOU Fengqin.Study on isozymes of farm cultivars of Trichosanthis fructus from Shandong[J].Journal of Chinese Medicinal Materials,2005,28(10):4-6.
[12] 厉娜,黄志刚,梁春,等.瓜蒌提取物治疗链脲佐菌素诱导糖尿病小鼠心肌纤维化的实验研究[J].中西医结合心脑血管病杂志,2016,14(4):355-357.LI Na,HUANG Zhigang,LIANG Chun,et al.Therapeutic effect of Trichosanthes fruit extract on myocardial fibrosis in streptozotocin induced diabetic mice[J].Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease,2016,14(4):355-357.
[13] 刘明,李慧,李陈跟,等.瓜蒌化学成分及药用价值的研究综述[J].阜阳师范学院学报(自然科学版),2018,35(1):23-26.LIU Ming,LI Hui,LI Chengen,et al.Chemical composition and pharmacological effects of Trichosanthes kirilowii[J].Journal of Fuyang Normal University(Natural Science),2018,35 (1):23-26.
[14] 邹纯才,吕文雅,鄢海燕,等.瓜蒌提取物的化学指纹特征及5种成分的定量分析[J].通化师范学院学报,2019,40(2):39-42.
[15] 刘金娜,温春秀,刘铭,等.瓜蒌的化学成分和药理活性研究进展[J].中药材,2013,36(5):843-848.
[16] 滕勇荣,张永清.瓜蒌化学成分研究进展[J].山东中医药大学学报,2011,35(1):85-86.
[2] 孙娟,孟冰雪,赵启韬.瓜蒌药理作用的物质基础研究概况[J].山东中医杂志,2012,31(6):461-463.
[3] 孙兴龙,段崇英.瓜蒌的药理作用及临床应用[J].中国药师杂志,2003,6(10):655-656.
[4] 屠婕红,余菁,陈伟光.瓜蒌的化学成分和药理作用研究概况[J].中国药师,2004,7(7):562-563.
[5] 张腾,张艳军,庄朋伟,等.瓜蒌对心梗后心衰大鼠心功能及心肌细胞凋亡的影响[J].中国药理学通报,2016,32(8):1183-1184.ZHANG Teng,ZHANG Yanjun,ZHUANG Pengwei,et al.Effects of Gualou on cardiac function and cardiomyocyte apoptosis in heart failure following myocardial infarction in rats[J].Chinese Pharmacological Bulletin,2016,32(8):1183-1184.
[6] 张弢,沈文芳,朱俊.瓜蒌对心血管系统的药理作用及临床应用[J].中国乡村医药杂志,2007(11):52.
[7] LI Hui,KOCHUN H,TSANG S Y,et al.The ethanol extract of Trichosanthis fructus promotes fetal hemoglobin production via p38 MAPK activation and ERK inactivation in K562 cells[J].Evidence-Based Complementary and Alternative Medicine,2011:10.1093/ecam/neq022.
[8] YU Xiankuo,TANG Liying,WU Hongwei,et al.Trichosanthis fructus:Botany,traditional uses,phytochemistry and pharmacology[J].Journal of Ethnopharmacology,2018,224:570-578.
[9] 范雪梅,陈刚,苏姗姗,等.瓜蒌化学成分的分离与鉴定[J].沈阳药科大学学报,2011,28(11):871-874.FAN Xuemei,CHEN Gang,SU Shanshan,et al.Isolation and identification of constituents from fruit of Trichosanthes kirilowii Maxim[J].Journal of Shenyang Pharmaceutical University,2011,28(11):871-874.
[10] 万丽娟,卢金清,许俊洁,等.瓜蒌子化学成分和药理作用的研究进展[J].中国药房,2015,26(31):4440-4443.
[11] 孙稚颖,李恩波,周凤琴.山东农家栽培瓜蒌品种的同工酶研究[J].中药材,2005,28(10):4-6.SUN Zhiying,LI Enbo,ZHOU Fengqin.Study on isozymes of farm cultivars of Trichosanthis fructus from Shandong[J].Journal of Chinese Medicinal Materials,2005,28(10):4-6.
[12] 厉娜,黄志刚,梁春,等.瓜蒌提取物治疗链脲佐菌素诱导糖尿病小鼠心肌纤维化的实验研究[J].中西医结合心脑血管病杂志,2016,14(4):355-357.LI Na,HUANG Zhigang,LIANG Chun,et al.Therapeutic effect of Trichosanthes fruit extract on myocardial fibrosis in streptozotocin induced diabetic mice[J].Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease,2016,14(4):355-357.
[13] 刘明,李慧,李陈跟,等.瓜蒌化学成分及药用价值的研究综述[J].阜阳师范学院学报(自然科学版),2018,35(1):23-26.LIU Ming,LI Hui,LI Chengen,et al.Chemical composition and pharmacological effects of Trichosanthes kirilowii[J].Journal of Fuyang Normal University(Natural Science),2018,35 (1):23-26.
[14] 邹纯才,吕文雅,鄢海燕,等.瓜蒌提取物的化学指纹特征及5种成分的定量分析[J].通化师范学院学报,2019,40(2):39-42.
[15] 刘金娜,温春秀,刘铭,等.瓜蒌的化学成分和药理活性研究进展[J].中药材,2013,36(5):843-848.
[16] 滕勇荣,张永清.瓜蒌化学成分研究进展[J].山东中医药大学学报,2011,35(1):85-86.