止泻木子中锥丝碱的提取工艺
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摘要
目的:优化止泻木子中锥丝碱的提取工艺,为止泻木子质量控制标准的建立奠定基础。方法:在单因素试验基础上,以提取时间、甲醇浓度、料液比为因素,以止泻木子中锥丝碱提取得率为响应值,采用三因素三水平响应曲面试验设计法,确定最佳提取工艺。结果:最佳提取工艺为提取时间70 min、甲醇浓度75%、料液比1∶9。结论:该提取工艺稳定、合理、预测性良好。
Objective: The process of extracting conessine from Holarrhena antidysenteriaca optimized for establishing the quality control standards of Holarrhena antidysenteriaca. Methods: Based on the results of single factor tests,we selected extraction time,methanol concentration and solid/liquid ratio as the experimental factors and used the extraction yield of conessine from as the response value. Results: The optimal extraction parameters were as follows: extraction time 70 min,methanol concentration 75%,solid/liquid ratio 1: 9. Conclusion: The extraction method is reliable,reasonable and highly predictable.
Objective: The process of extracting conessine from Holarrhena antidysenteriaca optimized for establishing the quality control standards of Holarrhena antidysenteriaca. Methods: Based on the results of single factor tests,we selected extraction time,methanol concentration and solid/liquid ratio as the experimental factors and used the extraction yield of conessine from as the response value. Results: The optimal extraction parameters were as follows: extraction time 70 min,methanol concentration 75%,solid/liquid ratio 1: 9. Conclusion: The extraction method is reliable,reasonable and highly predictable.
引文
[1]葛晓磊,范冬立,孙博航.藏药止泻木子的化学成分[J].沈阳药科大学学报,2014,31(12):950.
[2]孙绪丁,权富家,任松鹏,等.藏药材止泻木子质量标准研究[J].亚太传统医药,2015,11(8):20.
[3]吴香杰,金昌.蒙药止泻木的生药鉴定[J].中国民族民间医药杂志,2001,2(49):111.
[4]耿金峰.止泻木子的活性物质研究[D].大连:大连轻工业学院,2008.
[5]耿金峰,钱斯日古楞,王红英,等.止泻木子活性物质的提取工艺及抑菌作用[J].食品科技,2007(7):145.
[6]段东柱.三种药用植物中乙酰胆碱酯酶抑制活性成分的研究[D].兰州:兰州理工大学,2011.
[7] Dua V K,Verma G,Singh B,et al. Anti-malarial property of steroidal alkaloid conessine isolated from the bark of Holarrhena antidysenterica[J]. Malaria Journal,2013,12:194.
[8] Patrice B K,Veronique P B,David L,et al. Antibacterial activities of the extracts and conessine from Holarrhena floribunda G. Don.(Apocynaceae)[J]. African Journal of Traditional Complementary and Alternative Medicines,2007,4(3):352.
[9] Santra V J,Covel J A,Hayashi R,et al. A new family of H-3 receptor antagonists based on the natural product Conessine[J]. Bioorganic&Medicinal Chemistry Letters,2008,18(4):1490.
[10]冯玉康,赵伯友,姜国志.响应曲面法优化黄芩苷精制工艺研究[J].中国现代中药,2013,15(06):490-495.
[11]冯玉康,王文鹏,郝鹏彬,等.响应曲面法优化银杏叶提取物柱层析精制工艺[J].中国现代中药,2015,17(12):1321-1326.
[2]孙绪丁,权富家,任松鹏,等.藏药材止泻木子质量标准研究[J].亚太传统医药,2015,11(8):20.
[3]吴香杰,金昌.蒙药止泻木的生药鉴定[J].中国民族民间医药杂志,2001,2(49):111.
[4]耿金峰.止泻木子的活性物质研究[D].大连:大连轻工业学院,2008.
[5]耿金峰,钱斯日古楞,王红英,等.止泻木子活性物质的提取工艺及抑菌作用[J].食品科技,2007(7):145.
[6]段东柱.三种药用植物中乙酰胆碱酯酶抑制活性成分的研究[D].兰州:兰州理工大学,2011.
[7] Dua V K,Verma G,Singh B,et al. Anti-malarial property of steroidal alkaloid conessine isolated from the bark of Holarrhena antidysenterica[J]. Malaria Journal,2013,12:194.
[8] Patrice B K,Veronique P B,David L,et al. Antibacterial activities of the extracts and conessine from Holarrhena floribunda G. Don.(Apocynaceae)[J]. African Journal of Traditional Complementary and Alternative Medicines,2007,4(3):352.
[9] Santra V J,Covel J A,Hayashi R,et al. A new family of H-3 receptor antagonists based on the natural product Conessine[J]. Bioorganic&Medicinal Chemistry Letters,2008,18(4):1490.
[10]冯玉康,赵伯友,姜国志.响应曲面法优化黄芩苷精制工艺研究[J].中国现代中药,2013,15(06):490-495.
[11]冯玉康,王文鹏,郝鹏彬,等.响应曲面法优化银杏叶提取物柱层析精制工艺[J].中国现代中药,2015,17(12):1321-1326.