印楝素和银杏酚酸对甜菜夜蛾幼虫的协同增效作用
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摘要
研究印楝素和银杏酚酸复配对甜菜夜蛾幼虫的协同增效作用,为开发植物源农药提供理论依据。用印楝素和银杏酚酸及其复配混剂对甜菜夜蛾2龄幼虫进行室内毒力和拒食活性测定,筛选对甜菜夜蛾高效低毒的药剂。结果表明,印楝素和银杏酚酸复配质量比为6∶2,对甜菜夜蛾2龄幼虫具有明显的协同增效作用,2d后的致死中质量浓度(LC_(50))为20.31mg/L,共毒系数为295.36;当印楝素和银杏酚酸复配质量比为3∶5时,拒食活性测定共毒系数为225.22,AFC_(50)为27.03mg/L,增效作用最强。
The research aimed to determine the common-toxicity of the mixture of azadirachtin and ginkgolic acids and provide theoretical basis for developing natural pesticides.The toxicity and antifeeding of azadirachtin and ginkgolic acids and their admixture against the 2nd-instar larvae of Spodoptera exigua(Hübner)were conducted in laboratory.The results showed that azadirachtin and ginkgolic acids enhanced their toxicity with each other.The results showed that a mass ratio 6∶2 mixture of azadirachtin and ginkgolic acids was the most synergetic stomach poison with a co-toxicity coefficient of 295.36 two days later.Under this condition,the admixture had the most synergistic effect.The LC_(50) was 20.31 mg/L for up to 2d.The admixture produced amaximum anti-feeding effect and the AFC_(50) was 27.03mg/L when the admixture was blended with ginkgolic acids and azadirachtin at the mass ratio of 3∶5,and the most synergetic effect was detected with a co-toxicity coefficient of 225.22.
The research aimed to determine the common-toxicity of the mixture of azadirachtin and ginkgolic acids and provide theoretical basis for developing natural pesticides.The toxicity and antifeeding of azadirachtin and ginkgolic acids and their admixture against the 2nd-instar larvae of Spodoptera exigua(Hübner)were conducted in laboratory.The results showed that azadirachtin and ginkgolic acids enhanced their toxicity with each other.The results showed that a mass ratio 6∶2 mixture of azadirachtin and ginkgolic acids was the most synergetic stomach poison with a co-toxicity coefficient of 295.36 two days later.Under this condition,the admixture had the most synergistic effect.The LC_(50) was 20.31 mg/L for up to 2d.The admixture produced amaximum anti-feeding effect and the AFC_(50) was 27.03mg/L when the admixture was blended with ginkgolic acids and azadirachtin at the mass ratio of 3∶5,and the most synergetic effect was detected with a co-toxicity coefficient of 225.22.
引文
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[5]钟万芳,李雪萍,郭慧芳.一株高效防治甜菜夜蛾的莱氏野村菌的筛选[J].环境昆虫学报,2015,37(4):910-914.ZHONG W F,LI X P,GUO H F.The screening of a ley’s wild mushroom as effective control of the Spodoptera exigua[J].Journal of Environmental Insects,2015,37(4):910-914(in Chinese).
[6]沈舟兵,周义昌,郭建平,等.性诱剂对蔬菜斜纹夜蛾、甜菜夜蛾的防效分析[J].浙江农业科学,2014(12):1818-1819.SHEN ZH B,ZHOU Y CH,GUO J P,et al.Control effect of sex attractants on Spodoptera lituraand Spodoptera exigua[J].Journal of Zhejiang Agricultural Sciences,2014(12):1818-1819(in Chinese).
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[8]FLAMERY M C,SINTIM H O,RACZKOWSKI C W,et al.In biorational insecticides used on collard greens,Brassica oleracea,infested with diamondback moth,Plutella xylostella[J].Phytopathology,2011,101(6):53-57.
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[11]张秀丽,王炎,刘斌,等.桑叶提取物1-DNJ对甜菜夜蛾幼虫生物活性的影响[J].安徽农业科学,2013,41(33):12891-12893.ZHANG X L,WANG Y,LIU B,et al.Effects of 1-DNJextract from mulberry leaves on the biological activity of Spodoptera exigua[J].Anhui Agricultural Sciences,2013,41(33):12891-12893(in Chinese).
[12]张文彬,李红侠,伊尚武.生物农药的发展及其在防治甜菜夜蛾上的应用[J].中国甜菜糖业,2005(3):41-45.ZHANG W B,LI H X,YI SH W.Development of biological pesticides and its application in control of Spodoptera exigua[J].Chinese Beet Sugar Industry,2005(3):41-45(in Chinese).
[13]朱家颖,肖春,柯贤江,等.印楝素与鱼藤酮及其复配混剂对斜纹夜蛾的毒效研究[J].云南农业大学学报,2006,21(3):315-319.ZHU J Y,XIAO CH,KE X J,et al.Toxicity of azadirachtin and rotenone and its compound mixture to Spodoptera litura[J].Journal of Yunnan Agricultural Unversity,2006,21(3):315-319(in Chinese).
[14]杨小明,陈钧,钱之玉.烷基酚酸的生物活性研究进展[J].中草药,2003,34(5):5-6.YANG X M,CHEN J,QIAN ZH Y.Research progress on the bioactivity of alkyl phenolic acids[J].Chinese Herbal Medicine,2003,34(5):5-6(in Chinese).
[15]方静,谭卫红.来自银杏提取物的抗肿瘤化合物的研究进展[J].生物质化学工程,2008,42(5):56-60.FANG J,TAN W H.Advances in antitumor compounds fromGinkgo biloba extracts[J].Biomass Chemical Engineering,2008,42(5):56-60(in Chinese).
[16]李中新,孙绪艮.银杏酚酸及其防治农业害虫研究进展[J].山东农业大学学报(自然科学版),2007,38(4):654-656.LI ZH X,SUN X Y.Research progress of ginkgolic acids and its control of agricultural pests[J].Journal of Shandong Agricultural University(Natural Science Edition),2007,38(4):654-656(in Chinese).
[17]张宗炳.杀虫药剂的毒力测定[M].北京:科学出版杜,1988.ZHANG Z B.Toxicity of Insecticides[M].Beijing:Science Press,1988(in Chinese).
[18]张志祥,徐汉虹,程东美.EXCEL在毒力回归计算中的应用[J].昆虫知识,2002,39(1):67-70.ZHANG ZH X,XU H H,CHENG D M.Application of EXCEL in virulence regression calculation[J].Insect Knowledge,2002,39(1):67-70(in Chinese).
[19]陈立,徐汉虹.农药复配最佳增效配方筛选方法的探讨[J].植物保护学报,2000,27(4):349-354.CHEN L,XU H H.Discussion on the method of screening the best effective formula of pesticide compound[J].Journal of Plant Protection,2000,27(4):349-354(in Chinese).
[20]陈立,徐汉虹,赵善欢.甘蓝叶碟厚度对昆虫拒食剂拒食活性的影响[J].华中农业大学学报,2000,19(1):12-14.CHEN L,XU H H,ZHAO SH H.Effects of cabbage leaf thickness on antifeedant activity of insect antifeedant[J].Journal of Huazhong Agricultural University,2000,19(1):12-14(in Chinese).
[21]陈立,徐汉虹.唐古特瑞香对菜粉蝶幼虫的拒食和胃毒活性[J].天然产物研究与开发,2001,12(6):22-26.CHEN L,XU H H.Antifeedant and stomach poisoning activity of Tangguteruihong to juvenile butterfly[J].Research and Development of Natural Products,2001,12(6):22-26(in Chinese).
[22]SUN Y P,JOHNSON E R.Analysis of joint action of insecticides against house flies[J].Ecological Entomology,1960,53:887-891.
[23]PELHATE M,SATTELLE D.Pharmacological properties of insect axions[J].Insect Physiology,1982,28(11):889-903.
[24]ISAO KUBO,MUJOKIMet al.Protaglandin synthesase inhibitors from the African medicinal plant Ozorao mucronata[J].Chemistry Letters,1987,16(6):1101-1104.
[2]金党琴.银杏酚酸对甜菜夜蛾的生物活性及控制作用[J].河南农业科学,2013,42(1):79-82.JIN D Q.Bioactivity and control of ginkgolic acids on Spodoptera exigua[J].Journal of Henan Agricultural Sciences,2013,42(1):79-82(in Chinese).
[3]马建英.斜纹夜蛾、甘蓝夜蛾和甜菜夜蛾形态区分、发生规律与防治[J].河北农业,2015(1):26-27.MA J Y.Morphological distinction,occurrence regularity,and control of Prodenia litura,Mamestra brassicae Linnaeus and Spodoptera exigua[J].Hebei Agriculture,2015(1):26-27(in Chinese).
[4]钟国军,胡美英.黄杜鹃花提取物对甜菜夜蛾的生物活性[J].西北农林科技大学学报(自然科学版),2000,28(2):98-102.ZHONG G J,HU M Y.Bioactivity of Rhododendron fortunei extraction to Spodoptera exigua[J].Journal of Northwest A&F University(Natural Science Edition),2000,28(2):98-102(in Chinese wtih English abstract).
[5]钟万芳,李雪萍,郭慧芳.一株高效防治甜菜夜蛾的莱氏野村菌的筛选[J].环境昆虫学报,2015,37(4):910-914.ZHONG W F,LI X P,GUO H F.The screening of a ley’s wild mushroom as effective control of the Spodoptera exigua[J].Journal of Environmental Insects,2015,37(4):910-914(in Chinese).
[6]沈舟兵,周义昌,郭建平,等.性诱剂对蔬菜斜纹夜蛾、甜菜夜蛾的防效分析[J].浙江农业科学,2014(12):1818-1819.SHEN ZH B,ZHOU Y CH,GUO J P,et al.Control effect of sex attractants on Spodoptera lituraand Spodoptera exigua[J].Journal of Zhejiang Agricultural Sciences,2014(12):1818-1819(in Chinese).
[7]张海珍,郑霞娟.济源市甜菜夜蛾的发生规律及综合防治[J].河南农业,2014(19):30-31.ZHANG H ZH,ZHENG X J.Occurrence and control of Spodoptera exiguain Jiyuan city[J].Henan Agriculture,2014(19):30-31(in Chinese).
[8]FLAMERY M C,SINTIM H O,RACZKOWSKI C W,et al.In biorational insecticides used on collard greens,Brassica oleracea,infested with diamondback moth,Plutella xylostella[J].Phytopathology,2011,101(6):53-57.
[9]AHMED E M,ABD EL-MAGEED,SHEHATA E M.Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis(Boisd.)[J].Plant Protect,2011,47(4):166-175.
[10]韩鹏杰,高越,樊建斌,等.植物源农药在无公害农产品生产中的应用[J].植物保护导刊,2011,31(2):13-16.HAN P J,GAO Y,FAN J B,et al.Application of plant derived pesticides in production of pollution free agricultural products[J].Plant Protection Guide,2011,31(2):13-16(in Chinese).
[11]张秀丽,王炎,刘斌,等.桑叶提取物1-DNJ对甜菜夜蛾幼虫生物活性的影响[J].安徽农业科学,2013,41(33):12891-12893.ZHANG X L,WANG Y,LIU B,et al.Effects of 1-DNJextract from mulberry leaves on the biological activity of Spodoptera exigua[J].Anhui Agricultural Sciences,2013,41(33):12891-12893(in Chinese).
[12]张文彬,李红侠,伊尚武.生物农药的发展及其在防治甜菜夜蛾上的应用[J].中国甜菜糖业,2005(3):41-45.ZHANG W B,LI H X,YI SH W.Development of biological pesticides and its application in control of Spodoptera exigua[J].Chinese Beet Sugar Industry,2005(3):41-45(in Chinese).
[13]朱家颖,肖春,柯贤江,等.印楝素与鱼藤酮及其复配混剂对斜纹夜蛾的毒效研究[J].云南农业大学学报,2006,21(3):315-319.ZHU J Y,XIAO CH,KE X J,et al.Toxicity of azadirachtin and rotenone and its compound mixture to Spodoptera litura[J].Journal of Yunnan Agricultural Unversity,2006,21(3):315-319(in Chinese).
[14]杨小明,陈钧,钱之玉.烷基酚酸的生物活性研究进展[J].中草药,2003,34(5):5-6.YANG X M,CHEN J,QIAN ZH Y.Research progress on the bioactivity of alkyl phenolic acids[J].Chinese Herbal Medicine,2003,34(5):5-6(in Chinese).
[15]方静,谭卫红.来自银杏提取物的抗肿瘤化合物的研究进展[J].生物质化学工程,2008,42(5):56-60.FANG J,TAN W H.Advances in antitumor compounds fromGinkgo biloba extracts[J].Biomass Chemical Engineering,2008,42(5):56-60(in Chinese).
[16]李中新,孙绪艮.银杏酚酸及其防治农业害虫研究进展[J].山东农业大学学报(自然科学版),2007,38(4):654-656.LI ZH X,SUN X Y.Research progress of ginkgolic acids and its control of agricultural pests[J].Journal of Shandong Agricultural University(Natural Science Edition),2007,38(4):654-656(in Chinese).
[17]张宗炳.杀虫药剂的毒力测定[M].北京:科学出版杜,1988.ZHANG Z B.Toxicity of Insecticides[M].Beijing:Science Press,1988(in Chinese).
[18]张志祥,徐汉虹,程东美.EXCEL在毒力回归计算中的应用[J].昆虫知识,2002,39(1):67-70.ZHANG ZH X,XU H H,CHENG D M.Application of EXCEL in virulence regression calculation[J].Insect Knowledge,2002,39(1):67-70(in Chinese).
[19]陈立,徐汉虹.农药复配最佳增效配方筛选方法的探讨[J].植物保护学报,2000,27(4):349-354.CHEN L,XU H H.Discussion on the method of screening the best effective formula of pesticide compound[J].Journal of Plant Protection,2000,27(4):349-354(in Chinese).
[20]陈立,徐汉虹,赵善欢.甘蓝叶碟厚度对昆虫拒食剂拒食活性的影响[J].华中农业大学学报,2000,19(1):12-14.CHEN L,XU H H,ZHAO SH H.Effects of cabbage leaf thickness on antifeedant activity of insect antifeedant[J].Journal of Huazhong Agricultural University,2000,19(1):12-14(in Chinese).
[21]陈立,徐汉虹.唐古特瑞香对菜粉蝶幼虫的拒食和胃毒活性[J].天然产物研究与开发,2001,12(6):22-26.CHEN L,XU H H.Antifeedant and stomach poisoning activity of Tangguteruihong to juvenile butterfly[J].Research and Development of Natural Products,2001,12(6):22-26(in Chinese).
[22]SUN Y P,JOHNSON E R.Analysis of joint action of insecticides against house flies[J].Ecological Entomology,1960,53:887-891.
[23]PELHATE M,SATTELLE D.Pharmacological properties of insect axions[J].Insect Physiology,1982,28(11):889-903.
[24]ISAO KUBO,MUJOKIMet al.Protaglandin synthesase inhibitors from the African medicinal plant Ozorao mucronata[J].Chemistry Letters,1987,16(6):1101-1104.