药材唐古特瑞香中4种主要成分的含量测定与聚类分析
|
摘要
目的建立唐古特瑞香药材中4种主要成分的HPLC测定方法,并进行聚类分析。方法:采用高效液相色谱法。色谱柱为Teckmeck C_(18)(5μm,4.6 mm×250 mm);流动相A:乙腈,流动相B:0.4%磷酸,梯度洗脱(0～40 min,A:15%→30%;40～45 min,A:30%→15%;45～50 min,A:15%);检测波长为266 nm,流速1.0 mL·min~(-1),柱温为35℃,进样量为20μL。结果祖师麻甲素、芫花素、紫丁香苷、7-羟基香豆素检测质量浓度线性范围分别为0.4112～205.6μg·m L~(-1)(r=0.9999)、0.4348～217.40μg·m L~(-1)(r=0.9999)、0.4104～205.2μg·m L~(-1)(r=0.9999)、0.4276～213.80μg·m L~(-1)(r=0.9999);精密度、稳定性、重复性试验的RSD均<2.0%;加样回收率为97.4%～101.3%(RSD=0.80%～1.3%,n=6)。10批次样品可聚为2类,产地为青海海北州门源县与青海湟中县样品所含成分含量相近。结论建立的含量测定方法操作简单,精密度、稳定性、重复性好,可用于同时测定唐古特瑞香药材中祖师麻甲素、芫花素、紫丁香苷、7-羟基香豆素的含量;不同产地的唐古特瑞香药材成分含量差异较大。
Objective To establish a method for the simultaneous determination of 4 components in Daphne tangutica Maxim and to conduct cluster analysis. Methods HPLC method was adopted. The determination was performed on Teckmeck C_(18)(5 μm, 4.6 mm×250 mm) column with mobile phase consisting of acetonitrile-0.4% phosphate acid solution(gradient elution) at the flow rate of 1.0 mL·min~(-1). The detection wavelength was 266 nm and the column temperature was 35℃. The injection volumn was 20 μL. Results The linear ranges of daphnetin, genkwanin, syringin, and 7-hydroxycumarin were 0.4112-205.6 μg·m L~(-1)(r =0.9999), 0.4348-217.40 μg·mL~(-1)(r = 0.9999), 0.4104-205.2 μg·mL~(-1)(r = 0.9999), and 0.4276-213.80 μg·mL~(-1)(r = 0.9999), respectively. The RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.4%-101.3%(RSD = 0.80%-1.3%, n = 6). The 10 batches of medicinal materials could be divided into 2 categories. Conclusion The method is simple, precise, stable and reproducible, and it can be used for the simultaneous determination of 4 components in Daphne tangutica Maxim. The quality of Daphne tangutica Maxim from different regions is quite different.
Objective To establish a method for the simultaneous determination of 4 components in Daphne tangutica Maxim and to conduct cluster analysis. Methods HPLC method was adopted. The determination was performed on Teckmeck C_(18)(5 μm, 4.6 mm×250 mm) column with mobile phase consisting of acetonitrile-0.4% phosphate acid solution(gradient elution) at the flow rate of 1.0 mL·min~(-1). The detection wavelength was 266 nm and the column temperature was 35℃. The injection volumn was 20 μL. Results The linear ranges of daphnetin, genkwanin, syringin, and 7-hydroxycumarin were 0.4112-205.6 μg·m L~(-1)(r =0.9999), 0.4348-217.40 μg·mL~(-1)(r = 0.9999), 0.4104-205.2 μg·mL~(-1)(r = 0.9999), and 0.4276-213.80 μg·mL~(-1)(r = 0.9999), respectively. The RSDs of precision, stability and reproducibility tests were all lower than 2.0%. The recoveries were 97.4%-101.3%(RSD = 0.80%-1.3%, n = 6). The 10 batches of medicinal materials could be divided into 2 categories. Conclusion The method is simple, precise, stable and reproducible, and it can be used for the simultaneous determination of 4 components in Daphne tangutica Maxim. The quality of Daphne tangutica Maxim from different regions is quite different.
引文
[1]青海省药品检验所,青海省藏医药研究所.中国藏药[M].上海:上海科技出版社,1996:545-546.
[2]张薇,苏娟,扈晓佳,等.中药祖师麻的三种基源植物的化学及药理活性[J].中国医药工业杂志,2007,38(3):233-238.
[3]文慧,卢永昌. HPLC法对唐古特瑞香中瑞香素和芫花素含量的测定[J].湖北农业科学,2014,53(7):1654-1656.
[4]刘荣华,梅崇烨,邵峰,等.唐古特瑞香化学成分研究[J].中药材,2009,32(12):83-87.
[5]高宁.青海省海北州唐古特瑞香的药用价值及栽培技术[J].畜牧与饲料科学,2012,33(10):1846-1845.
[6]刘亚蓉.唐古特瑞香中祖师麻甲素的HPLC法含量测定[J].陕西中医,2009,30(1):85-87.
[7]荚丽丽,魏岚,赵健.了哥王药材中7种成分的含量测定与聚类分析[J].中国药房,2017,28(33):4706-4710.
[8]郭全兴,刘亚蓉,杨凤梅,等.山莨菪麝香膏质量标准研究[J].中成药,2010,32(4):692-694.
[9]文怀宇,郑爽,郎轶咏,等.蓝萼香茶菜药材的质量标准研究[J].中南药学,2018,16(2):233-237.
[10]王永香,米慧娟,李淼,等.不同产地栀子药材中8种主要药效成分的含量测定及聚类分析[J].中国实验方剂学杂志,2015,21(20):44-48.
[11]陈奕君,赵薇,丁玲,等.芳香新塔花和小新塔花全草中4个成分含量测定及聚类分析[J].药物分析杂志,2015,35(11):1908-1913.
[12]张颖,龙全江,徐雪琴.不同产地延胡索中生物碱类成分的含量测定及质量分析[J].中药材,2017,40(1):73-76.
[13]郭元满,刘爱华,张妮瑜,等.不同产地枳壳药材中柠檬苦素、诺米林含量测定及聚类分析[J].中南药学,2018,16(1):104-106.
[2]张薇,苏娟,扈晓佳,等.中药祖师麻的三种基源植物的化学及药理活性[J].中国医药工业杂志,2007,38(3):233-238.
[3]文慧,卢永昌. HPLC法对唐古特瑞香中瑞香素和芫花素含量的测定[J].湖北农业科学,2014,53(7):1654-1656.
[4]刘荣华,梅崇烨,邵峰,等.唐古特瑞香化学成分研究[J].中药材,2009,32(12):83-87.
[5]高宁.青海省海北州唐古特瑞香的药用价值及栽培技术[J].畜牧与饲料科学,2012,33(10):1846-1845.
[6]刘亚蓉.唐古特瑞香中祖师麻甲素的HPLC法含量测定[J].陕西中医,2009,30(1):85-87.
[7]荚丽丽,魏岚,赵健.了哥王药材中7种成分的含量测定与聚类分析[J].中国药房,2017,28(33):4706-4710.
[8]郭全兴,刘亚蓉,杨凤梅,等.山莨菪麝香膏质量标准研究[J].中成药,2010,32(4):692-694.
[9]文怀宇,郑爽,郎轶咏,等.蓝萼香茶菜药材的质量标准研究[J].中南药学,2018,16(2):233-237.
[10]王永香,米慧娟,李淼,等.不同产地栀子药材中8种主要药效成分的含量测定及聚类分析[J].中国实验方剂学杂志,2015,21(20):44-48.
[11]陈奕君,赵薇,丁玲,等.芳香新塔花和小新塔花全草中4个成分含量测定及聚类分析[J].药物分析杂志,2015,35(11):1908-1913.
[12]张颖,龙全江,徐雪琴.不同产地延胡索中生物碱类成分的含量测定及质量分析[J].中药材,2017,40(1):73-76.
[13]郭元满,刘爱华,张妮瑜,等.不同产地枳壳药材中柠檬苦素、诺米林含量测定及聚类分析[J].中南药学,2018,16(1):104-106.