便携式人工心肺辅助装置的体外氧合性能测试
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摘要
目的通过体外实验考察便携式人工心肺辅助装置的氧合性能。方法应用肝素涂层中空纤维氧合技术与磁悬浮离心泵装置整合构建小型化、便携式心肺辅助装置,采用红细胞比容0.30的牛血进行体外氧合效率检测试验,分别在0.5~5.0 L/min流量运转检测装置流入道及流出道血氧饱和度,氧分压,二氧化碳分压等,计算装置氧合效率。并进行各流量12 h运转游离血红蛋白检测评价装置溶血性能。结果装置流出道氧饱和度在各流量点均保持98%以上。装置氧合效率与转速及流量呈正相关,P<0.01。在流量0.5~5.0 L/min,装置运行12 h,游离血红蛋白含量均在20 g/L以下。结论应用肝素涂层中空纤维氧合器与磁悬浮离心血泵构建便携式整合动力氧合装置氧合性能可靠。
Objective To evaluate the invitrooxygenation capacity of a wearable artificial cardiopulmonary support device.Methods Heparin-coating hollow fiber and axial pump were used to setup a wearable artificial cardiopulmonary support device. Invitrooxygenation was investigated by experimental circulation with fresh bovine blood in 0.30 HCT. The saturation, partial oxygen pres-sure and partial carbon dioxide pressure of inlet and outlet were measured, and oxygen transfer rate was calculated. The plasma free he-moglobin was evaluated as well.ResultsThe saturation of outlet were stable at over 98% at different flow rates of 0.5-5.0 L/ min. Theoxygen transfer rate was correlated with the flow rate. The plasma free hemoglobin was below 20 mg/ dL during a running period of 12 hours.Conclusion The wearable artificial cardiopulmonary support device with heparin-coating hollow fiber and axial pump has a re-liable oxygenative effect invitro.
Objective To evaluate the invitrooxygenation capacity of a wearable artificial cardiopulmonary support device.Methods Heparin-coating hollow fiber and axial pump were used to setup a wearable artificial cardiopulmonary support device. Invitrooxygenation was investigated by experimental circulation with fresh bovine blood in 0.30 HCT. The saturation, partial oxygen pres-sure and partial carbon dioxide pressure of inlet and outlet were measured, and oxygen transfer rate was calculated. The plasma free he-moglobin was evaluated as well.ResultsThe saturation of outlet were stable at over 98% at different flow rates of 0.5-5.0 L/ min. Theoxygen transfer rate was correlated with the flow rate. The plasma free hemoglobin was below 20 mg/ dL during a running period of 12 hours.Conclusion The wearable artificial cardiopulmonary support device with heparin-coating hollow fiber and axial pump has a re-liable oxygenative effect invitro.
引文
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[2]Hutchens M,Song H,Ibsen L.Extracorporeal membrane oxygenation for ARDS in adults.N Engl J Med,2012,366(6):575.
[3]Dipchand AI,Kirk R,Edwards LB,et al.The Registry of the International Society for Heart and Lung Transplantation:Sixteenth Official Pediatric Heart Transplantation Report--2013;focus theme:age.J Heart Lung Transplant,2013,32(10):979-988.
[4]Benden C,Edwards LB,Kucheryavaya AY,et al.The Registry of the International Society for Heart and Lung Transplantation:Sixteenth Official Pediatric Lung and Heart-Lung Transplantation Report--2013;focus theme:age.J Heart Lung Transplant,2013,32(10):989-997
[5]Schmidt F,Sasse M,Boehne M,et al.Concept of"awake venovenous extracorporeal membrane oxygenation"in pediatric patients awaiting lung transplantation.Pediatr Transplant,2013,17(3):224-230.
[6]Zhou K,Niu S,Bianchi G,et al.Biocompatibility assessment of a long-term wearable artificial pump-lung in sheep.Artif Organs,2013,37(8):678-688.
[7]Zhang T,Wei X,Bianchi G,et al.A novel wearable pump-lung device:in vitro and acute in vivo study.J Heart Lung Transplant,2012,31(1):101-105
[8]Wu ZJ,Zhang T,Bianchi G,et al.Thirty-day in-vivo performance of a wearable artificial pump-lung for ambulatory respiratory support.Ann Thorac Surg,2012,93(1):274-281.
[9]Hou X,Guo L,Zhan Q,et al.Extracorporeal membrane oxygenation for critically ill patients with 2009 influenza A(H1N1)-related acute respiratory distress syndrome:preliminary experience from a single center.Artif Organs,2012,36(9):780-786.
[10]ARDS Definition Task Force,Ranieri VM,Rubenfeld GD,et al.Acute respiratory distress syndrome:the Berlin Definition.JAMA,2012,307(23):2526-2533.
[11]Organization ELS.ECLS Registry Report International Summary July,2012.2012.
[12]Javidfar J,Brodie D,Iribarne A,et al.Extracorporeal membrane oxygenation as a bridge to lung transplantation and recovery.J Thorac Cardiovasc Surg,2012,144(3):716-721.
[13]Rollins MD,Hubbard A,Zabrocki L,et al.Extracorporeal membrane oxygenation cannulation trends for pediatric respiratory failure and central nervous system injury.J Pediatr Surg,2012,47(1):68-75.