葛甘咀嚼片有效成分提取工艺的研究
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摘要
目的建立葛甘咀嚼片有效成分含量测定方法,优化葛甘咀嚼片有效成分提取工艺。方法采用高效液相色谱法进行有效成分的含量测定,色谱条件:Agilent TC-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.05%磷酸水溶液(B)梯度洗脱(0→10→30→40→45 min;15%→15%→30%→70%→15%乙腈),流速1.0 mL/min,柱温30℃,检测波长为254 nm。采用正交设计,以提取物中有效成分含量、出膏率为指标,用综合评分法进行数据处理,优化提取工艺条件。结果五味子醇甲、甘草苷、甘草酸铵、葛根素的线性范围良好,方法学考察符合规定;葛甘咀嚼片的最佳提取工艺为8倍量70%乙醇提取1次,1 h/次。结论本研究建立的含量测定方法符合方法学验证要求,可用于葛甘咀嚼片有效成分的测定,有效成分提取工艺条件合理、稳定。
Objective To optimize the extraction process of Gegan Chewable Tablet. Methods The analysis was performed on a Agilent TC-C_(18) column(4.6 mm×250 mm 5 μm) with gradient elution(0→10→30→40→45 min;15%→15%→30%→70%→15%A)by using the mobile phase of acetonitrile(A)-0.01% phosphoric acid solution(B).The flow rate was 1.0 mL/min and the column temperature was 30 ℃,the detection wavelengths was 254 nm.The effective components and the extraction ratio were employed as target in the extracted solution.An orthogonal design was adopted in the study.Data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition. Results The linear ranges of 4 index components were well,and methodological investigation meets the requirements.The ethanol extraction process was composed of 70% ethanol,solid-liquid ratio 1∶8,extraction time 60 min,one hours of each. Conclusion This method meets the method validation requirements,and can simultaneously determine the index components of Gegan Chewable Tablet,while the optimized process is stable and feasible.
Objective To optimize the extraction process of Gegan Chewable Tablet. Methods The analysis was performed on a Agilent TC-C_(18) column(4.6 mm×250 mm 5 μm) with gradient elution(0→10→30→40→45 min;15%→15%→30%→70%→15%A)by using the mobile phase of acetonitrile(A)-0.01% phosphoric acid solution(B).The flow rate was 1.0 mL/min and the column temperature was 30 ℃,the detection wavelengths was 254 nm.The effective components and the extraction ratio were employed as target in the extracted solution.An orthogonal design was adopted in the study.Data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition. Results The linear ranges of 4 index components were well,and methodological investigation meets the requirements.The ethanol extraction process was composed of 70% ethanol,solid-liquid ratio 1∶8,extraction time 60 min,one hours of each. Conclusion This method meets the method validation requirements,and can simultaneously determine the index components of Gegan Chewable Tablet,while the optimized process is stable and feasible.
引文
[1] 王亚红,李战英.甘草酸的提取和精制法研究现状[J].天津化工,2003,17(6):34-37.
[2] 苑可武,白芳,杨波,等.甘草酸的提取和精制法概述[J].中国医药工业杂志,2002,33(7):362-364.
[3] 金文姗,谈钰元,陈有根,等.高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量[J].中国中药杂志,2003,28(1):49-51.
[4] 丰理象,洪小军,庄林芳.葛根中葛根素提取工艺的优化[J].中草药,2003,34(9):812-813.
[5] 李琰,范璐,孙秀华.葛根提取工艺的考察[J].北京中医药大学学报,2001,24(4):26-27.
[6] 彭菊艳,王俊儒,张义英,等.葛根有效成分的含量测定与提取工艺优化[J].陕西农业科学,2005(3):52-54.
[7] 朱华,刘芯蕊,王孝勋,等.葛花的研究进展[J].中医药学刊,2005,23(12):2273-2274.
[8] 李翠,徐必学,张永萍,等.甘草中甘草苷的含量测定方法研究[J].时珍国医国药,2009,20(3):564-565.
[9] 范彩玲,张海燕,周映霞,等.甘草中甘草酸提取方法的比较[J].河南科学,2010,28(12):1543-1545.
[10] 崔淑芬,张信青,许柏球,等.高效液相色谱法同时测定甘草中甘草酸和甘草苷的含量[J].时珍国医国药,2006,17(3):307-308.
[11] 周文斌.葛根总黄酮提取工艺条件的研究[J].渝州大学学报(自然科学版),2002,19(12):9-11.
[2] 苑可武,白芳,杨波,等.甘草酸的提取和精制法概述[J].中国医药工业杂志,2002,33(7):362-364.
[3] 金文姗,谈钰元,陈有根,等.高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量[J].中国中药杂志,2003,28(1):49-51.
[4] 丰理象,洪小军,庄林芳.葛根中葛根素提取工艺的优化[J].中草药,2003,34(9):812-813.
[5] 李琰,范璐,孙秀华.葛根提取工艺的考察[J].北京中医药大学学报,2001,24(4):26-27.
[6] 彭菊艳,王俊儒,张义英,等.葛根有效成分的含量测定与提取工艺优化[J].陕西农业科学,2005(3):52-54.
[7] 朱华,刘芯蕊,王孝勋,等.葛花的研究进展[J].中医药学刊,2005,23(12):2273-2274.
[8] 李翠,徐必学,张永萍,等.甘草中甘草苷的含量测定方法研究[J].时珍国医国药,2009,20(3):564-565.
[9] 范彩玲,张海燕,周映霞,等.甘草中甘草酸提取方法的比较[J].河南科学,2010,28(12):1543-1545.
[10] 崔淑芬,张信青,许柏球,等.高效液相色谱法同时测定甘草中甘草酸和甘草苷的含量[J].时珍国医国药,2006,17(3):307-308.
[11] 周文斌.葛根总黄酮提取工艺条件的研究[J].渝州大学学报(自然科学版),2002,19(12):9-11.