结合拉曼微测技术的TLC-SERS鉴别槲皮素与芦丁
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摘要
目的:研究槲皮素与芦丁的薄层色谱-表面增强拉曼光谱(TLC-SERS),用于中药中有效成分的鉴别。方法:通过实验考察,确定了激发光波长;采用拉曼微测技术分别扫描涂有表面增强剂的槲皮素与芦丁TLC斑点,获得二维平面分布像图,将激光束快速定位于样品与表面增强剂活位结合点上,得到相应的SERS;并与常规拉曼光谱对比。结果:以780 nm激光为激发波长,银溶胶为表面增强剂,通过比较分析槲皮素与芦丁的TLC-SERS光谱,建立了黄蜀葵花、槐花中有效成分的二维TLC-SERS鉴别法。结论:本方法专属性强,灵敏度高,重复性好,可用于中药的鉴别。
Objective:To study the TLC-SERS of quercetin and rutin for the identification of active ingredients in traditional Chinese medicine. Methods:Through the experimental investigation,the wavelength of the excitation light was selected.The Raman micro-measurement technique was used to scan the spots of quercetin and rutin coated with surface enhancer toobtaina two-dimensional distribution image plane. The corresponding conding point of SERS which was produced by rapid positioning of the laser beam on the sample surface with the enhancer-binding activities was obtained.And then,the results were compared with the ones acquired by regular Raman spectroscopy. Results:The TLC-SERS of quercetin and rutin were established with excitation wavelength at 780 nm,surface enhancer of silver colloids and micro-Raman measurement technology,further successfully applied to identify quercetin in Abelmoschi Corolla and rutin in Sophorae Flos.Conclusion:The method established in this study is found to have significant specificity,high sensitivity and good reproducibility,and can be applied to the identification of traditional Chinese medicine.
Objective:To study the TLC-SERS of quercetin and rutin for the identification of active ingredients in traditional Chinese medicine. Methods:Through the experimental investigation,the wavelength of the excitation light was selected.The Raman micro-measurement technique was used to scan the spots of quercetin and rutin coated with surface enhancer toobtaina two-dimensional distribution image plane. The corresponding conding point of SERS which was produced by rapid positioning of the laser beam on the sample surface with the enhancer-binding activities was obtained.And then,the results were compared with the ones acquired by regular Raman spectroscopy. Results:The TLC-SERS of quercetin and rutin were established with excitation wavelength at 780 nm,surface enhancer of silver colloids and micro-Raman measurement technology,further successfully applied to identify quercetin in Abelmoschi Corolla and rutin in Sophorae Flos.Conclusion:The method established in this study is found to have significant specificity,high sensitivity and good reproducibility,and can be applied to the identification of traditional Chinese medicine.
引文
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[2]林静.芦丁的临床药理特点[J].中国临床药理学杂志,2009,25(3):256LIN J.Clinical pharmaceutical special of rutin[J].Chin J Clin Pharmacol,2009,25(3):256
[3]延玺,刘会青,邹永青,等.黄酮类化合物生理活性及合成研究进展[J].有机化学,2008,28(9):1534YAN X,LIU HQ,ZOU YQ,et al.Physiological activities and research advance in synthesis of flavonoids[J].Chin J Org Chem,2008,28(9):1534
[4]中华人民共和国药典2015年版.一部[S].2015:354ChP 2015.VolⅠ[S].2015:354
[5]张雁,尹利辉,冯芳.拉曼光谱分析法的应用介绍[J].药物分析杂志,2009,29(7):1236ZHANG Y,YIN LH,FENG F.Introduct the application of Raman scattering method[J].Chin J Pharm Anal,2009,29(7):1236
[6]朱自莹,顾仁敖,陆天虹.拉曼光谱在化学中的应用[M].沈阳:东北大学出版社,1998:71ZHU ZY,GU RA,LU TH.Apply of Raman Spectroscopy in Chemistry[M].Shenyang:Press of Northeast University,1998:71
[7]JOHN RF,KAZUO N,CHRIS WB.Introductory Raman Spectroscopy[M].New York:Academic Press,2002:211
[8]苏松柏,张永萍,张丽丽,等.拉曼光谱在中药质量控制中的应用[J].中国实验方剂学杂志,2011,17(8):284SU SB,ZHANG YP,ZHANG LL,et al.Review on Raman spectroscopy application in quality control of traditional Chinese medicine[J].Chin J Exp Tradit Med Form,2011,17(8):284
[9]王玉,李忠红,张正行,等.拉曼光谱在药物分析中的应用[J].药学学报,2004,39(9):764WANG Y,LI ZH,ZHANG ZX,et al.Applications of Raman spectroscopy in pharmaceutical analysis[J].Acta Pharm Sin,2004,39(9):764
[10]贾宝申,哈斯乌力吉,林翔,等.表面增强拉曼光谱活性基底的制备及其应用研究[J].食品安全质量检测学报,2014,5(5):1490JIA BS,HASI WLJ,LIN X,et al.Preparation of Raman surfaceenhanced scattering substrate and its application[J].J Food Safe Qual,2014,5(5):1490
[11]SANCI R,VOLKAN M.Surface-enhanced Raman scattering(SERS)studies on silver nanorod substrates[J].Sens Actuators B,2009,139(1):150
[12]方芳.纳米银溶胶的制备优化及TLC-Dynamic SERS方法研究[D].上海:第二军医大学,2016FANG F.Preparation and Optimization of Silver Colloids and the Study of TLC-Dynamic Method[D].Shanghai:The Second Military Medical University,2016
[13]李秀明,李金阳,刘新兰.纳米银溶胶稳定性的考察及其对表面增强拉曼光谱检测的影响[J].药物分析杂志,2013,33(6):1048LI XM,LI JY,LIU XL.Stability of nano silver particles and the influence on surface-enhanced Raman spectroscopic detection[J].Chin J Pharm Anal,2013,33(6):1048
[14]ZHENG J,HE LL.Surface-enhanced Raman spectroscopy for the chemical analysis of food[J].Compr Rev Food Sci Food Saf,2014,13(3):317
[15]SCHLUCKER S.Surface-enhanced Raman spectroscopy:conceptsand chemical applications[J].Angew Chem Int Ed,2014,53(19):4756