芍药苷对脂多糖诱导的人牙周膜细胞损伤的保护作用及机制研究
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摘要
目的:探讨芍药苷对脂多糖(LPS)诱导的人牙周膜细胞(hPDLCs)损伤的保护作用及机制。方法:分离hPDLCs并鉴定和培养,将hPDLCs分为对照组、模型组和芍药苷低、中、高剂量组,芍药苷作用24h后测定各组细胞增殖率,检测细胞中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-ɑ(TNF-ɑ)、基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)表达水平。结果:与对照组比较,模型组和各芍药苷剂量组细胞增殖率降低,hPDLCs细胞中IL-6、IL-1β、TNF-ɑ、MMP-2和MMP-9水平增高,差异均具有统计学意义(P<0.05);与模型组比较,各芍药苷剂量组细胞增殖率增加,且随着芍药苷剂量的增加,增加幅度越大,hPDLCs细胞中IL-6、IL-1β、TNF-ɑ、MMP-2和MMP-9水平降低,且随着芍药苷剂量的增加,增加/降低幅度越大,差异均具有统计学意义(P<0.05)。结论:芍药苷能抑制炎症因子(IL-6、IL-1β、TNF-ɑ)和基质金属蛋白酶-2、9的表达,减轻LPS诱导的hPDLCs细胞损伤。
Objective: To investigate the protective effect and mechanism of paeoniflorin in lipopolysaccharide( LPS)-induced injury of human periodontal ligament cells( hPDLCs). Methods: After the identification and culture,hPDLCs were divided into five groups including the control group,the model group,and low-dose,middle-dose and high-dose paeoniflorin groups. The cell proliferation rate and the expression levels of interleukin( IL-6),interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α),matrix metalloproteinase-2( MMP-2) and matrix metalloproteinase-9( MMP-9) were detected and compared among the groups. Results: Compared with that in the control group,the cell proliferation rate of the model group and each paeoniflorin group decreased,while the levels of IL-6,IL-1 beta,TNF-α,MMP-2 and MMP-9 in hPDLCs cells increased with statistical significance( P < 0. 05). Compared with that in the model group,the cell proliferation rate of each paeoniflorin group increased,and was positively correlated with the dose of paeoniflorin. With the increase of paeoniflorin dosage,the levels of IL-6,IL-1 beta,TNF-α,MMP-2 and MMP-9 in hPDLCs decreased. The increase/decrease was greater when the dosage of paeoniflorin was increased,and the difference was statistically significant( P <0. 05). Conclusion: The protective effect and mechanism of paeoniflorin in LPS-induced injury of hPDLCs may be related to the decrease of IL-6,IL-1β,TNF-α,MMP-2 and MMP-9.
Objective: To investigate the protective effect and mechanism of paeoniflorin in lipopolysaccharide( LPS)-induced injury of human periodontal ligament cells( hPDLCs). Methods: After the identification and culture,hPDLCs were divided into five groups including the control group,the model group,and low-dose,middle-dose and high-dose paeoniflorin groups. The cell proliferation rate and the expression levels of interleukin( IL-6),interleukin-1β( IL-1β),tumor necrosis factor-α( TNF-α),matrix metalloproteinase-2( MMP-2) and matrix metalloproteinase-9( MMP-9) were detected and compared among the groups. Results: Compared with that in the control group,the cell proliferation rate of the model group and each paeoniflorin group decreased,while the levels of IL-6,IL-1 beta,TNF-α,MMP-2 and MMP-9 in hPDLCs cells increased with statistical significance( P < 0. 05). Compared with that in the model group,the cell proliferation rate of each paeoniflorin group increased,and was positively correlated with the dose of paeoniflorin. With the increase of paeoniflorin dosage,the levels of IL-6,IL-1 beta,TNF-α,MMP-2 and MMP-9 in hPDLCs decreased. The increase/decrease was greater when the dosage of paeoniflorin was increased,and the difference was statistically significant( P <0. 05). Conclusion: The protective effect and mechanism of paeoniflorin in LPS-induced injury of hPDLCs may be related to the decrease of IL-6,IL-1β,TNF-α,MMP-2 and MMP-9.
引文
1中华口腔医学会牙周病学专业委员会.重度牙周炎诊断标准及特殊人群牙周病治疗原则的中国专家共识[J].中华口腔医学杂志,2017,52(2):67-71
2 Cutler CW,Shinedling EA,Nunn M,et al.Association between periodontitis and hyperlipidemia:cause or effect?[J].Journal of Periodontology,2017,70(12):1429-1434
3 Huang Z,Stabler T,Pei F,et al.Both systemic and local lipopolysaccharide(LPS)burden is associated with knee osteoarthritis(OA)[J].Osteoarthritis&Cartilage,2016,24(1):S329-S330
4 王莉,陈彦龙,徐召理.瑞舒伐他汀联合常规治疗急性脑梗死疗效及对患者血清VS-2、s ICAM-1和MMp2水平影响[J].中国药师,2016,19(6):1120-1123
5 Chen P,Xuan DY,Zhang JC.Periodontitis aggravates kidney damage in obese mice by MMP2 regulation[J].Bratisl Lek Listy,2017,48(5):740-745
6 Ajmera DH,Singh P,Ying Z,et al.A meta-analysis of MMP-9 promoter 1562 C/T polymorphism on susceptibility of chronic periodontitis[J].Springerplus,2016,5(1):1-10
7 储姗姗,查良平,段海燕,等.中国芍药组7种植物根的生长轮及其在赤芍类药材鉴别中的应用[J].中国中药杂志,2017,42(19):3723-3727
8 刘玲,赵建龙.芍药苷对脂多糖诱导的小鼠急性肝损伤的保护作用[J].中国临床药理学杂志,2016,32(5):433-436
9 Shi D,Wang Q,Zheng H,et al.Paeoniflorin suppresses IL-6/Stat3pathway via upregulation of Socs3 in dendritic cells in response to 1-chloro-2,4-dinitrobenze[J].International Immunopharmacology,2016,38(12):45-53
10 辛月娇,刘一颖,徐屹.中性粒细胞功能的研究进展及其平衡失调与牙周炎的关系[J].华西口腔医学杂志,2016,34(2):210-214
11 Yin D,Liu YY,Wang TX,et al.Paeoniflorin exerts analgesic and hypnotic effects via adenosine A 1,receptors in a mouse neuropathic pain model[J].Psychopharmacology,2016,233(2):281-293
12 Li Y,Shen R,Wen G,et al.Effects of ketamine on levels ofi Inflammatory cytokines IL-6,IL-1β,and TNF-αin the hippocampus of mice following acute or chronic administration[J].Frontiers in Pharmacology,2017,8(38771):139-153
13 Albrecht LJ,Tauber SC,Merres J,et al.Lack of proinflammatory cytokine interleukin-6 or tumor necrosis factor receptor-1 results in a failure of the innate immune response after bacterial meningitis[J].Mediators of Inflammation,2016,6(2):1-12
14 Arévalo-Romero H,Meza I,Vallejo-Flores G,et al.Helicobacter pylori Cag A and IL-1βpromote the epithelial-to-mesenchymal transition in a nontransformed epithelial cell model[J].Gastroenterology Research and Practice,2016,6(1):1-10
15 Hong S,Li R,Xu Q,et al.Two types of TNF-αexist in teleost fish:phylogeny,expression,and bioactivity analysis of type-II TNF-α3 in rainbow trout Oncorhynchus mykiss[J].Journal of Immunology,2016,191(12):5959-5972
16 Kapoor C,Vaidya S,Wadhwan V,et al.Seesaw of matrix metalloproteinases(MMPs)[J].Journal of Cancer Research&Therapeutics,2016,12(1):28-35
17 Jabnońskatrypu A,Matejczyk M,Rosochacki S.Matrix metalloproteinases(MMPs),the main extracellular matrix(ECM)enzymes in collagen degradation,as a target for anticancer drugs[J].J Enzyme Inhib Med Chem,2016,23(1):177-183
18 Hsiao YF,Yang LC,Chou YS,et al.Matrix metalloproteinase-2,-9 ,and tissue inhibitor of MMP-2 gene polymorphisms in Taiwanese periodontitis patients[J].Journal of Dental Sciences,2016,11(4):411-418
19 张冠军,张巍,王开成,等.厄贝沙坦对糖尿病大鼠心肌纤维化中基质金属蛋白酶通路及相关因子的影响[J].中国糖尿病杂志,2017,15(12):1124-1128
2 Cutler CW,Shinedling EA,Nunn M,et al.Association between periodontitis and hyperlipidemia:cause or effect?[J].Journal of Periodontology,2017,70(12):1429-1434
3 Huang Z,Stabler T,Pei F,et al.Both systemic and local lipopolysaccharide(LPS)burden is associated with knee osteoarthritis(OA)[J].Osteoarthritis&Cartilage,2016,24(1):S329-S330
4 王莉,陈彦龙,徐召理.瑞舒伐他汀联合常规治疗急性脑梗死疗效及对患者血清VS-2、s ICAM-1和MMp2水平影响[J].中国药师,2016,19(6):1120-1123
5 Chen P,Xuan DY,Zhang JC.Periodontitis aggravates kidney damage in obese mice by MMP2 regulation[J].Bratisl Lek Listy,2017,48(5):740-745
6 Ajmera DH,Singh P,Ying Z,et al.A meta-analysis of MMP-9 promoter 1562 C/T polymorphism on susceptibility of chronic periodontitis[J].Springerplus,2016,5(1):1-10
7 储姗姗,查良平,段海燕,等.中国芍药组7种植物根的生长轮及其在赤芍类药材鉴别中的应用[J].中国中药杂志,2017,42(19):3723-3727
8 刘玲,赵建龙.芍药苷对脂多糖诱导的小鼠急性肝损伤的保护作用[J].中国临床药理学杂志,2016,32(5):433-436
9 Shi D,Wang Q,Zheng H,et al.Paeoniflorin suppresses IL-6/Stat3pathway via upregulation of Socs3 in dendritic cells in response to 1-chloro-2,4-dinitrobenze[J].International Immunopharmacology,2016,38(12):45-53
10 辛月娇,刘一颖,徐屹.中性粒细胞功能的研究进展及其平衡失调与牙周炎的关系[J].华西口腔医学杂志,2016,34(2):210-214
11 Yin D,Liu YY,Wang TX,et al.Paeoniflorin exerts analgesic and hypnotic effects via adenosine A 1,receptors in a mouse neuropathic pain model[J].Psychopharmacology,2016,233(2):281-293
12 Li Y,Shen R,Wen G,et al.Effects of ketamine on levels ofi Inflammatory cytokines IL-6,IL-1β,and TNF-αin the hippocampus of mice following acute or chronic administration[J].Frontiers in Pharmacology,2017,8(38771):139-153
13 Albrecht LJ,Tauber SC,Merres J,et al.Lack of proinflammatory cytokine interleukin-6 or tumor necrosis factor receptor-1 results in a failure of the innate immune response after bacterial meningitis[J].Mediators of Inflammation,2016,6(2):1-12
14 Arévalo-Romero H,Meza I,Vallejo-Flores G,et al.Helicobacter pylori Cag A and IL-1βpromote the epithelial-to-mesenchymal transition in a nontransformed epithelial cell model[J].Gastroenterology Research and Practice,2016,6(1):1-10
15 Hong S,Li R,Xu Q,et al.Two types of TNF-αexist in teleost fish:phylogeny,expression,and bioactivity analysis of type-II TNF-α3 in rainbow trout Oncorhynchus mykiss[J].Journal of Immunology,2016,191(12):5959-5972
16 Kapoor C,Vaidya S,Wadhwan V,et al.Seesaw of matrix metalloproteinases(MMPs)[J].Journal of Cancer Research&Therapeutics,2016,12(1):28-35
17 Jabnońskatrypu A,Matejczyk M,Rosochacki S.Matrix metalloproteinases(MMPs),the main extracellular matrix(ECM)enzymes in collagen degradation,as a target for anticancer drugs[J].J Enzyme Inhib Med Chem,2016,23(1):177-183
18 Hsiao YF,Yang LC,Chou YS,et al.Matrix metalloproteinase-2,-9 ,and tissue inhibitor of MMP-2 gene polymorphisms in Taiwanese periodontitis patients[J].Journal of Dental Sciences,2016,11(4):411-418
19 张冠军,张巍,王开成,等.厄贝沙坦对糖尿病大鼠心肌纤维化中基质金属蛋白酶通路及相关因子的影响[J].中国糖尿病杂志,2017,15(12):1124-1128